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Citations for Invivogen :
1 - 50 of 141 citations for Goat Anti Mouse IgG Fc Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Secreted Embryonic Alkaline Phosphatase (SEAP) (Invivogen) were purchased for various cell processing and analysis experiments ...
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bioRxiv - Synthetic Biology 2023Quote: ... or HRP-conjugated anti-human IgG Fc (Invivogen; 31413). Proteins from transient transfections were either purified via His-tag or Protein A purification ...
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bioRxiv - Synthetic Biology 2023Quote: ... HRP-conjugated anti-human IgG Fc antibodies (Invivogen; 31413) were used for detection ...
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bioRxiv - Synthetic Biology 2023Quote: ... 50uL of HRP-conjugated anti-human IgG Fc (Invivogen; 31413) diluted 1:5000 in blocking buffer was added to the plates and incubated at room temperature for 1hr ...
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bioRxiv - Synthetic Biology 2023Quote: ... Western blots using HRP-conjugated anti-human IgG Fc (Invivogen; 31413) diluted 1:10000 in blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... Anti-human IgG Fc Capture (AHC) Biosensors tips were initially loaded with Dectin-1A:FC fusion protein (Invivogen, #fc-hdec1a) at 13 ug/ml ...
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bioRxiv - Microbiology 2020Quote: ... reporter cell supernatants were analyzed for secreted alkaline phosphatase activity using HEK-Blue Detection reagent (Invivogen). Cytokines were quantified by enzyme-linked immunosorbent assay following the manufacturer’s protocol (R&D Systems) ...
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bioRxiv - Microbiology 2023Quote: ... and TLR9 activation was quantified by measuring secreted embryonic alkaline phosphatase (SEAP) by QuantiBlueTM reagent (Invivogen) using a microplate reader (BioTek Synergy HI ...
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bioRxiv - Microbiology 2023Quote: HEK-BlueTM hTLR2 secreted alkaline phosphatase (SEAP) reporter assays were performed according to manufacturer instructions (Invivogen, hkb-htlr2). Briefly ...
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bioRxiv - Immunology 2020Quote: ... The supernatants were discarded before staining with 50 μL of 2 μg/mL secondary AF488 goat anti rabbit (goat IgG H+L, Invivogen) in cytometry buffer for 30 min on ice ...
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bioRxiv - Biochemistry 2019Quote: ... Supernatant from HEK-Blue IFN-α/β cells was then assayed for alkaline phosphatase activity using QUANTI-Blue (InvivoGen), following manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... the human monocyte cell line THP1-Blue expressing an NF-κB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene (InvivoGen) were cultured in RPMI-1640 (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The secreted embryonic alkaline phosphatase (SEAP) was measured in a microplate reader (OD Ex/Em = 620/655 nm) using Quanti-Blue Solution (InvivoGen), following the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: HEK-Blue cells expressing human or murine TLR9 and carrying the NF-κB SEAP (secreted embryonic alkaline phosphatase) reporter gene (InvivoGen) were used according to the manufacturer’s instructions and adapted to assess TLR-specific NF-κB activity induced via live C ...
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bioRxiv - Microbiology 2024Quote: ... NF-κB activity was monitored by the production of secreted embryonic alkaline phosphatase (SEAP) through the hydrolysis of HEK-Blue (InvivoGen). Optical density was read at 650 nm using a SpectraMax340 PC plate reader (Molecular Devices).
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bioRxiv - Microbiology 2022Quote: ... and TLR9 and TLR7 activation was determined by measuring secreted embryonic alkaline phosphatase (SEAP) reporter activity in cell culture supernatants by QuantiBlue™ reagent (Invivogen) using a microplate reader (BioTek Synergy HI ...
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bioRxiv - Synthetic Biology 2021Quote: SEAP was detected in the media and in the lysate with an alkaline phosphatase detection medium – powder QUANTI-Blue™ (InvivoGen) as per the products instructions ...
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bioRxiv - Immunology 2022Quote: ... Cells were co-cultured for 24h and cell-cell fusion was assessed measuring secreted embryonic alkaline phosphatase (SEAP) activity into cells supernatant using QUANTI-Blue™ Solution (Invivogen), according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2020Quote: ... 20 µL of cell culture supernatant was then withdrawn and analyzed for alkaline phosphatase content via change in Quanti-Blue (Invivogen, rep-qbs) absorbance at 620 nm (Spectramax id3 plate reader).
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bioRxiv - Immunology 2023Quote: ... anti-hIL-6-IgG (Invivogen) or mouse IgG1 kappa Isotype Control (eBioscience ...
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bioRxiv - Immunology 2020Quote: ... NF-kB reporter monocytic cells (THP-1-XBlue) stably expressing a secreted embryonic alkaline phosphatase (SEAP) reporter inducible by NF-κB were purchased from InvivoGen (InvivoGen, San Diego, CA, USA). - ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: THP1-Blue™ NF-κB cells (derived from the human THP-1 monocyte cell line by stable integration of an NF-kB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene (InvivoGen, San Diego, CA, USA; 2 × 105 per well) were cultured in 96 well plate using RPMI containing 10 % heat inactivated FBS ...
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bioRxiv - Bioengineering 2022Quote: ... For FCM assay using hACE2-Fc (Invivogen, Catalogue code: fc-hace2), 5μL of the protein was incubated for 1 hour at 4°C with the cells using a similar assay condition described above ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 S1-614D and S1-614G tagged with human IgG Fc fragment were constructed by insertion of the S1 region (residues 1-681) to pFUSE-hIgG1-Fc1 vector (InvivoGen, USA) and expressed using the Expi293 Expression system (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... fused with the cDNA sequence of the mouse hinge and IgG2a heavy chain Fc region (pFUSE-mIgG2a-Fc1, InvivoGen), downstream of a mouse Ig kappa chain secretion signal peptide and was subcloned into a pMSGV retroviral vector followed by enhanced (e)GFP reporter gene cassette using PCR and standard molecular cloning techniques to generate pMSGV-A4-Fc-T2A-eGFP ...
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bioRxiv - Microbiology 2023Quote: ... Soluble hACE2-Fc (fchace2, InvivoGen), a recombinant protein consisting of the extracellular domain of human ACE2 fused to a human IgG1 Fc region ...
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bioRxiv - Microbiology 2020Quote: ... Soluble Fc-mDectin-1a containing the C-terminal extracellular domain of mouse Dectin-1a fused with the human IgG1 Fc domain was purchased from Invivogen. Overnight C ...
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bioRxiv - Immunology 2023Quote: ... and another 1 μg/ml of anti-hIL-6-IgG (Invivogen) or mouse IgG1 kappa Isotype Control were added to the wells after 2 days of co-culture.
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bioRxiv - Cell Biology 2021Quote: ... Targets for inhibition were TLR4 (anti-TLR4 IgG, 1 μg/ml; Invivogen); interleukin-1 receptor-associated kinase 1 and 4 (IRAK 1/4 ...
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bioRxiv - Biochemistry 2020Quote: ... ACE2-8xHis-AviTag plasmids were generated by subcloning the Spike-RBD or ACE2 DNA fragment into a pFUSE-hIgG1-Fc-AviTag vector (adapted from the pFUSE-hIgG1-Fc vector from InvivoGen). The ACE2-Fc-LgBiT fusion plasmids were generated by subcloning the gene fragments of LgBiT to the N- or C-terminus of the ACE2-TEV-Fc-AviTag vector with a 10-amino acid (N-terminal fusion ...
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bioRxiv - Bioengineering 2020Quote: ... VH-Fc were cloned into a pFUSE (InvivoGen) vector with a human IgG1 Fc domain ...
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bioRxiv - Immunology 2023Quote: ... 10% FCS and 250µg/ml Hygromycin B gold (InvivoGen). Virus stock production was performed under BSL-3 conditions using DMEM medium supplemented with glutamax and 2% FCS ...
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bioRxiv - Neuroscience 2023Quote: ... Rat neutralizing monoclonal anti-mDECTIN-1-IgG (clone R1-4E4) (Invivogen mabg-mdect, 1:30); Rat monoclonal anti-CD206 (clone MR5D3 ...
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bioRxiv - Microbiology 2019Quote: ... Mice were also subcutaneously immunized with 20 μg of Gn-Fc or Gc-Fc protein absorbed in aluminum hydroxychloride (Alhydrogel® adjuvant 2%, InvivoGen, Hong Kong). Mice sera were collected from immunized mice at one week after the third immunization to determine the levels of specific antibody titers ...
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bioRxiv - Microbiology 2019Quote: ... Primary antibodies used: Mouse anti-HA antibody (InvivoGen, 1:1000), rabbit anti-ACP (1:2000) ...
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bioRxiv - Immunology 2020Quote: ... + 1% FCS and treated with 500 ng/mL Pam3CSK4 (InvivoGen) for 4 hours and 10 μM nigericin (InvivoGen ...
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bioRxiv - Immunology 2021Quote: ... IgA+ cells were detected by addition of goat anti-human IgA F(ab’)2 (InVivoGen) followed by anti-goat IgG AlexaFluor 594 (Jackson Immunoresearch ...
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bioRxiv - Immunology 2021Quote: ... The plates were then incubated with sequentially 1:5 serially diluted hACE2-Fc (invivogen) and two anti-SARS-CoV-2 RBD mAbs (clones H4 [InvivoGen] and CR3022 [Abcam]) starting at 10 μg/ml in blocking buffer ...
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bioRxiv - Immunology 2021Quote: ... IgG4 using IgG plasmids (Invivogen) by conventional cloning techniques as previously described (35 ...
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bioRxiv - Bioengineering 2020Quote: ... The Fc control protein was derived from the pFuse-IgG1e3-Fc2 (InvivoGen). For the generation of full length PSG1-Fc and PSG9-Fc ...
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bioRxiv - Physiology 2022Quote: ... aeruginosa flagellin (FLA-PA) and soluble TLR5 ectodomain (hTLR5-Fc) were from InvivoGen. Dulbecco’s phosphate buffered saline (DPBS ...
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bioRxiv - Microbiology 2022Quote: ... the cells are resuspended in FACS buffer containing 5ng/μL Fc-hDectin1 (Invivogen) and incubated in the dark on ice for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... Wako Chemicals) and rat anti-mouse Clec7a (activated microglia; 1:50; InvivoGen, CA, USA) in 2% NDS and PBS-T for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... They were incubated overnight in blocking buffer solution with the primary Anti-mDectin-1-IgG antibody (1:50; cat# mabg-mdect, Invivogen) at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed twice with PBS and stained with Fc-hDectin-1a (Invivogen) and Calcofluor white (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... M25 scFv was subcloned into either the Fc fusion expression vector pFUSE-hIgG1 Fc2 (InvivoGen) or a modified Abvec expression vector that was originally provided by Dr ...
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bioRxiv - Immunology 2019Quote: ... into expression plasmids adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK2 vectors (Invivogen). Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: Human TLR4 reporter cell line HEK-TLR4 cells containing a nuclear factor κ-light-chain-enhancer of activated B cells (NFκB)-inducible secreted embryonic alkaline phosphate (SEAP) reporter (InvivoGen; San Diego, CA) were used to assess the effect of resistin on TLR4 activation as previously reported15 ...
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bioRxiv - Immunology 2022Quote: ... normal rat PAb IgG control blocking antibodies (InvivoGen), Dexamethasone (10µM ...
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bioRxiv - Immunology 2022Quote: ... mouse TLR2 neutralizing antibody monoclonal mouse IgG2a (C9A12) and control isotypes mouse IgG2a were from InvivoGen. Mouse TLR4/MD2 complex neutralizing antibody monoclonal rat IgGk clone MTS510 and control isotypes rat IgGk were from eBioscience™ Invitrogen ...