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Citations for Biotium Inc. :
1 - 50 of 86 citations for rno mir 542 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... Positive bands of appropriate size were confirmed using individual primer sets and resultant PCR products were visualized on a 1.5% agarose gel with gel red (Biotium).
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed using EvaGreen (Biotium, Catalog no: 89138-982) and the Biorad CFX Connect Real-time PCR system ...
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bioRxiv - Genetics 2019Quote: ... quantitative PCR (RT-qPCR) was performed on cDNA (described above) using Eva Green (Biotium) on a Step-One Plus Real time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... and the RT-PCR products were analysed by standard agarose gel electrophoresis visualized by GelRed Nucleic Acid Stain (Biotium) and subjected to Sanger sequencing (core facility of the Haartman Institute ...
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bioRxiv - Synthetic Biology 2019Quote: ... The primers (Microsynth, Balgach) were used in the Real-Time PCR reaction with Fast EvaGreen® qPCR Master Mix (Biotium, USA) on ABI StepOnePlus (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting cDNA was diluted five-fold and RT-qPCR was performed with Eva™ Green PCR Master Mix (Biotium, Fremont, CA) in a StepOne Plus Real-Time PCR Platform (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: To differentiate between viable and non-viable cells and viruses in qPCR, half of the aliquots mentioned above (i.e., the second set of triplicates) received a PMAxx dye treatment (Biotium, Fremont, CA). Aliquots of the biomass samples (230 μL ...
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bioRxiv - Genomics 2019Quote: ... according to the manufacturer’s protocol with barcoded P5 and P7 sequencing primers at 0.5 mM and with 1x EvaGreen dye (Biotium) and 1x ROX reference dye (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... rinsed in PBS for 10 min at RT and blocked using blocking buffer (Fish Gelatin blocking agent, BIOTIUM) in the dark for 1 hr ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were visualised with GelRed (Biotium, USA) gel electrophoresis and gel-purified ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were visualized with GelRed dye (Biotium) after agarose gel electrophoresis using a GelDoc imaging system (BioRad).
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bioRxiv - Microbiology 2020Quote: ... The 10 µL qPCR reactions contained 1 µL of sample and 9 µL of master mix (0.25 mM of each primer, 1X Forget-Me-Not EvaGreen qPCR mix (Biotium, Fremont, CA), and 0.4 mg mL-1 BSA ...
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bioRxiv - Genomics 2022Quote: A PMAxx Real-Time PCR Bacterial Viability Test (Biotium Inc.), designed for selective detection of viable S ...
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bioRxiv - Bioengineering 2024Quote: ... A typical reaction contained 100 nM of each primer and either 150 nM fluorescent probe or lx EvaGreen dye (Biotium, Fremont, CA, USA).
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bioRxiv - Developmental Biology 2021Quote: ... and qPCR was performed using Eva Green PCR Master Mix (Biotium) from nine regenerating fragments in four (Figure 4.2 ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were analyzed with 1.5% agarose gel containing GelRed (Biotium) in a 1X TAE buffer and subsequently captured the gel images using G:Box EF2 (Syngene ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were allowed to cure protected from light at RT for at least one day before the short edges of the coverglass only were sealed with CoverGrip (Biotium #23005, Fremont, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... 5 minutes each with PBS and incubated for 10 minutes at RT with a 1:200 dilution in PBS of the lipophilic dye CellBrite™ (Biotium. Cat. Nº: 30023). Finally ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were visualized by electrophoresis on agarose gel containing GelRed (Biotium) and band sizes were evaluated against a 100 bp molecular weight marker (NEB) ...
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bioRxiv - Molecular Biology 2019Quote: ... The qPCR was performed using EvaGreen Real-time PCR (Biotium, Hayward, USA) according to the manufacturer’s recommendations using 1µl EvaGreen Real-time PCR (Biotium ...
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bioRxiv - Molecular Biology 2022Quote: PCR products were mixed with 1x GelRed prestain loading buffer (Biotium #41010), separated on a 2% or 4% agarose gel (Seakem LE agarose ...
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bioRxiv - Immunology 2021Quote: ... PCR products were run on 1% agarose gel and visualised using GelRed (Biotium) and transgene sequence confirmed by Sanger sequencing at the Australian Genome Research Foundation (AGRF).
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were run on a 1.2% agarose gel containing GelRed™ (Biotium). After electrophoresis only a single band was observed ...
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bioRxiv - Developmental Biology 2022Quote: ... qRT-PCR was run on a ViiA 7 with SYBR green mastermix (Biotium). 18S was used as the human cell housekeeping gene and Ywhaz was used as the mouse housekeeping gene ...
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bioRxiv - Plant Biology 2023Quote: ... PCR products were analyzed on a 1 % agarose gel containing GelRed (0.01%, Biotium) and visualized under UV light.
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bioRxiv - Genetics 2023Quote: ... The PCR products were resolved on a 1.5% agarose gel containing GelRed (Biotium) and visualized under UV light to distinguish expanded from non-expanded CAG repeats ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were resolved on 1.0% agarose gel containing GelRed (Biotium, diluted 1:10,000) and imaged on a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Plant Biology 2022Quote: ... Quantitative real-time PCR was performed in 10 µL reactions with EvaGreen Mastermix (Biotium) using kit-specified reaction parameters in a Quant Studio 5 Real-Time PCR machine (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... Indexing was performed using PCR-mixture-2 blue (AmpliSens, Russia) and EvaGreen (Biotium, USA) as a dye ...
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bioRxiv - Zoology 2019Quote: ... PCR fragments were size-separated in 1.5% agarose gels and visualized with GelRed (Biotium). The resulting banding pattern of X (~975 bp ...
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bioRxiv - Genetics 2021Quote: ... Quantitative PCR (qPCR) was preformed using the Forget-Me-Not qPCR Master Mix (Biotium) with a BioRad iCycler thermocycler ...
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bioRxiv - Microbiology 2021Quote: PCR products were checked in 2% agarose gels stained with GelRed™ (Biotium, USA) under UV light ...
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bioRxiv - Molecular Biology 2019Quote: ... according to the manufacturer’s recommendations using 1µl EvaGreen Real-time PCR (Biotium, Hayward, USA), 1 µl of cell culture ...
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bioRxiv - Microbiology 2022Quote: ... PCR reactions were analyzed by electrophoresis using a 2% agarose gel containing GelRed (Biotium) and visualized using an Azure c400 imager (Azure Biosystems) ...
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bioRxiv - Zoology 2022Quote: All PCR products were verified on a 2% agarose gel stained with GelRed (Biotium). From each of the 12 libraries (2*3 replicates for MiFish-U and 2*3 replicates for Mamm01 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR products were run and visualized on a 1.5% agarose gel using gelgreen (BIOTIUM). To reduce the number of samples for subsequent purification ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR amplification was verified by visualizing on a 1 % agarose gel with GelRed (Biotium) and 1 kb Hyperladder (Bioline) ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products were run in 1.5% agarose gel electrophoresis stained with GelRed (Biotium, Hayward, USA).
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were visualized on a 2% agarose gel using GelRed™ (Biotium, Freemont, CA) before multiplexing with consideration to dye color and allele size (Table S1) ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time PCR was conducted using non-specific DNA-binding dye EvaGreen (Biotium, #31000) to quantitate the total amount of amplification products ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were visualized on agarose gels (1%) stained with Gel Red (Biotium Inc., USA). The PCR products were sequenced using BigDye Terminator v3.1 cycle sequencing kit and cleaned with BigDye XTerminator purification kit (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... PCR products were visualized on a 1% agarose gel with GelRed Nucleic Acid Stain (Biotium). PCR products were purified using Nucleospin Gel and PCR Clean-up kit (Takara) ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... PCR products were electrophorized on 2% agarose gels containing GelRed® (Biotium, Fremont, CA, USA) and images were captured using the ChemiDoc(tm ...
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bioRxiv - Plant Biology 2023Quote: ... PCR products were run in 2 % w/v agarose gels stained with Gel Red (Biotium).
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bioRxiv - Evolutionary Biology 2024Quote: ... The PCR products were revealed and confirmed by the GelRed Nucleic Acid Stain 10000X (Biotium) and photographed with UV transillumination using the VWR Genosmart gel documentation system after loading on 1.5% agarose (Nippon Genetics Europe GmbH)-TAE gels.
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bioRxiv - Genomics 2024Quote: ... and the purified PCR products were quantified using the AccuBlue NextGen dsDNA Quantitation Kit (Biotium) reagents and a Spark microplate reader (Tecan Life Sciences) ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were run on a 1.2% agarose gel containing GelRed™ (Biotium, Fremont, California) to confirm their length ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The PCR product was run on 2% agarose gel followed by staining with GelRed (Biotium, USA). The band size between 137nt–149nt was selected and purified using Gel Extraction Kit (Macherey-Nagel ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR products were resolved by agarose gel electrophoresis and visualized using GelGreen stain (Biotium cat#: 41005) on an ImageQuant LAS 4000 machine (GE Healthcare) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were visualized on a 1.5% agarose gel stained with GelRed® (Biotium, CA, USA). Bands of the expected size were purified using the Purelink Gel extraction kit (Thermo Fisher Scientific ...