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Citations for Biotium Inc. :
1 - 50 of 208 citations for rno mir 134 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: A PMAxx Real-Time PCR Bacterial Viability Test (Biotium Inc.), designed for selective detection of viable S ...
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bioRxiv - Molecular Biology 2019Quote: ... The qPCR was performed using EvaGreen Real-time PCR (Biotium, Hayward, USA) according to the manufacturer’s recommendations using 1µl EvaGreen Real-time PCR (Biotium ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative real-time PCR was performed in 10 µL reactions with EvaGreen Mastermix (Biotium) using kit-specified reaction parameters in a Quant Studio 5 Real-Time PCR machine (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: ... according to the manufacturer’s recommendations using 1µl EvaGreen Real-time PCR (Biotium, Hayward, USA), 1 µl of cell culture ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time PCR was conducted using non-specific DNA-binding dye EvaGreen (Biotium, #31000) to quantitate the total amount of amplification products ...
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bioRxiv - Genetics 2023Quote: ... Quantitative real-time PCR assays were performed on the Bio-Rad CFX96 instrument using Evagreen (Biotium, Freemont, CA, USA) with a procedure of 98 °C 3min ...
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bioRxiv - Genetics 2020Quote: To test the relative expression of XIST across cell lines quantitative real-time PCR (qPCR) was carried out using EVAgreen (Biotium) and Maxima Hot Start Taq (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2019Quote: ... The primers (Microsynth, Balgach) were used in the Real-Time PCR reaction with Fast EvaGreen® qPCR Master Mix (Biotium, USA) on ABI StepOnePlus (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed using EvaGreen (Biotium, Catalog no: 89138-982) and the Biorad CFX Connect Real-time PCR system ...
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bioRxiv - Microbiology 2022Quote: ... or CF594 TUNEL Assay Apoptosis Detection Kit (Biotium) according to the manufacturers’ protocols ...
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bioRxiv - Genetics 2019Quote: ... quantitative PCR (RT-qPCR) was performed on cDNA (described above) using Eva Green (Biotium) on a Step-One Plus Real time PCR system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2021Quote: The JC-1 mitochondrial membrane potential detection kit (Biotium, Hayward, CA, USA) was used for measuring mitochondrial membrane potential in oocytes ...
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bioRxiv - Microbiology 2023Quote: ... with either AccuGreen broad-range or high-sensitivity dsDNA detection kits (Biotium). To preserve mutant abundance ratios and reduce the propagation of PCR errors ...
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bioRxiv - Cell Biology 2023Quote: ... Frozen sections were processed with TUNEL apoptosis detection kit-CF594(catalog #30064, Biotium) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... and the RT-PCR products were analysed by standard agarose gel electrophoresis visualized by GelRed Nucleic Acid Stain (Biotium) and subjected to Sanger sequencing (core facility of the Haartman Institute ...
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bioRxiv - Cancer Biology 2021Quote: A JC-1 (5,5,6,6’-tetrachloro-1,1’,3,3’ tetraethylbenzimi-dazoylcarbocyanine iodide) mitochondrial membrane potential detection kit (Biotium, 30001) was used to measure mitochondrial membrane potential changes in live cells ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting cDNA was diluted five-fold and RT-qPCR was performed with Eva™ Green PCR Master Mix (Biotium, Fremont, CA) in a StepOne Plus Real-Time PCR Platform (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: TUNEL assay was used to detect cell apoptosis using the CF605R TUNEL Assay Apoptosis Detection Kit (Biotium) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Apoptotic DNA fragmentation was examined using 30064 CF™ 594 Dye TUNEL Assay Apoptosis Detection Kit (Biotium, Cedarlane) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... JC-1 Mitochondrial Membrane Potential Detection Kit and MitoView™ Green were purchased from Biotium (Hayward, CA, USA). Mouse monoclonal anti-α-Tubulin-FITC antibody and anti-phospho-Histone H2A.X (Ser139 ...
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bioRxiv - Genomics 2023Quote: Fluorescent probe detection was performed by hybridization of readout detection probes (100 nM) and DAPI (Biotium #S36936) in hybridization buffer (2X SSC ...
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bioRxiv - Cell Biology 2024Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was performed using TUNEL assay apoptosis detection kit per the manufacturer’s instructions (Biotium). Slides were mounted in Vectashield Antifade Mounting Medium (Vector Labs) ...
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bioRxiv - Molecular Biology 2020Quote: ... Readout detection probe hybridization: This is followed by hybridization of readout detection probes (100 nM) and DAPI (Biotium) in hybridization buffer for 2 hours at RT ...
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bioRxiv - Microbiology 2022Quote: ... for 24 h and stained with a JC-1 Mitochondrial Membrane Potential Detection Kit using the manufacturers protocol (Biotium, Fremont, CA). The dye forms JC aggregates (orange ...
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bioRxiv - Immunology 2021Quote: ... TUNEL staining was also performed with 25 μm thick floating tissue sections with the CF 640R TUNEL Assay Apoptosis Detection Kit (Biotium, Cat# 30074). Antibodies used for staining are indicated in Supplementary Table 1.
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bioRxiv - Immunology 2019Quote: ... Apoptosis assays were performed using Nucleocounter NC-3000 and a Flexicyte apoptosis/necrosis detection kit based on staining with caspase 3 substrate NucView 488 and RedDot 2 (all Biotium, Cambridge Bioscience, UK). Apoptotic cells are defined as NucView488 positive and RedDot2 negative ...
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bioRxiv - Genomics 2024Quote: ... and the purified PCR products were quantified using the AccuBlue NextGen dsDNA Quantitation Kit (Biotium) reagents and a Spark microplate reader (Tecan Life Sciences) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the concentration of the PCR products was quantified using the Accugreen High Sensitivity dsDNA Quantitation Kit (Biotium 31068). Sequencing libraries were then pooled equally and sequenced on a NextSeq500 Mid flow cell (150 bp single-end reads).
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bioRxiv - Cancer Biology 2022Quote: ... followed by the hybridization of readout detection probes (100 nM) and DAPI (Biotium) in the hybridization buffer for 1h at RTThe sections were washed with PBS and mounted with SlowFade Gold Antifade Mountant (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the concentration of the second round PCR products was quantified using the Accugreen High Sensitivity dsDNA Quantitation Kit (Biotium 31068). These libraries were then normalized ...
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bioRxiv - Plant Biology 2022Quote: All the PCR products were quantified with the AccuClear® Ultra High Sensitivity dsDNA quantification kit (Biotium, Fremont, CA, USA) and subpooled by sample type ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by hybridization of readout detection probes (100 nM) and DAPI (Biotium) in hybridization buffer for 1h at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... This was followed by hybridization of readout detection probes (100 nM) and DAPI (Biotium) in hybridization buffer for 1 h at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... Pink/Red-Gal substrate for B-gal activity detection (lineage tracing) was purchased from Biotium (10012).
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bioRxiv - Developmental Biology 2023Quote: ... we used the Tyramide signal amplification (TSA) detection system (PerkinElmer, MA, USA, or Biotium, Inc. CA, USA) as per the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... rinsed in PBS for 10 min at RT and blocked using blocking buffer (Fish Gelatin blocking agent, BIOTIUM) in the dark for 1 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were then washed three times and treated with TrueBlack lipofuscin quencher (Biotium) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were visualised with GelRed (Biotium, USA) gel electrophoresis and gel-purified ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were visualized with GelRed dye (Biotium) after agarose gel electrophoresis using a GelDoc imaging system (BioRad).
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bioRxiv - Developmental Biology 2020Quote: ... NucView Caspase-3 substrate detection (green or red label to complement fluorescent hemocyte reporters) was performed according to the manufacturer’s instructions (Biotium); cells were incubated for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Organoids were washed three times with PBS and stained with DID (2μg/ml, Biotium, 60014) overnight at 15°C ...
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bioRxiv - Microbiology 2020Quote: ... at a final concentration of 20 μg/ml and incubated in the dark for 2 min before detection or with CellBrite™ Fix 488 Membrane Dye (Biotium) at a final concentration of 10X (from a 1:1000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated for 1 h at RT with the secondary antibodies HRP goat anti-mouse (1:200 in 1% BSA-PBS) followed by 10 min amplification reaction with tyramide for the detection of Ngn3 (Tyramide Amplification Kid, 33003, Biotium, USA), or Alexa 594 donkey anti-rabbit for the detection of Pomc/Npy (1:1000 in 1% BSA-PBS ...
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bioRxiv - Genomics 2021Quote: ... washed three times with PBS followed by autofluorescence quenching with TrueBlack® Lipofuscin Autofluorescence Quencher (Biotium) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed several times for 2hrs in BB and mounted on glass with EverBrite (Biotium). For primary and secondary antibodies see Immunofluorescence microscopy and antibodies section.
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bioRxiv - Developmental Biology 2021Quote: ... and qPCR was performed using Eva Green PCR Master Mix (Biotium) from nine regenerating fragments in four (Figure 4.2 ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were analyzed with 1.5% agarose gel containing GelRed (Biotium) in a 1X TAE buffer and subsequently captured the gel images using G:Box EF2 (Syngene ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... ; Blots were washed 3 times in TBS/0.1% Tween and incubated with secondary IRD-tagged antibodies (CF770, Biotium) diluted 1:10,000 in Odyssey blocking buffer for 1 h at room temperature.
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bioRxiv - Neuroscience 2020Quote: ... Slides were allowed to cure protected from light at RT for at least one day before the short edges of the coverglass only were sealed with CoverGrip (Biotium #23005, Fremont, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... 5 minutes each with PBS and incubated for 10 minutes at RT with a 1:200 dilution in PBS of the lipophilic dye CellBrite™ (Biotium. Cat. Nº: 30023). Finally ...