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Citations for Biotium Inc. :
1 - 31 of 31 citations for Transglutaminase Substrate biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 70 μg of RNA was biotinylated with MTSEA biotin-XX (Biotium) as previously described (Chan et al. ...
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bioRxiv - Immunology 2021Quote: ... Oxidized vicinal diols were subsequently biotinylated with 1 mM biocytin hydrazide (Biotium) in the presence of 10 mM aniline (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... biotinylated Donkey anti-Sheep IgG (for ChAT, 1:250, #20191-1, Biotium). All antibodies were diluted in the blocking solution.
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bioRxiv - Cell Biology 2021Quote: ... and then fragmented with NaOH and biotinylated with MTSEA biotin-XXlinker (Biotium). The biotinylated RNA was then purified using µMACS Streptavidine MicroBeads (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2021Quote: ... The mixture was biotinylated with 10 µg of MTSEA biotin-XX (Biotium) in 100 mM HEPES/pH 7.5 and 10 mM EDTA for 30 min at 25°C in dark with gentle agitation and the biotinylated RNA was recovered by ethanol precipitation ...
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bioRxiv - Cancer Biology 2023Quote: ... 5µM NucView 488 caspase-3 substrate (Biotium, 30029) and media to 50µl was added ...
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bioRxiv - Cell Biology 2024Quote: ... The Renilla Luciferase substrate coelenterazine 400a (Biotium, Fremont, CA) was added to each well to a final concentration of 5µM and BRET signal was read after 2 minutes incubation at RT ...
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bioRxiv - Immunology 2024Quote: ... or NucView® Caspase-3 Substrate (Biotium, Fremont, CA). For staining with MitoSpy ...
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bioRxiv - Molecular Biology 2020Quote: ... and the remaining 4sU-labeled nascent RNA was biotinylated using MTSEA-Biotin-XX (Biotium, 90066) as previously described67,81 ...
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bioRxiv - Microbiology 2020Quote: ... and the remaining 4sU-labeled nascent RNA was biotinylated using MTSEA-Biotin-XX (Biotium; 90066) as previously described83 ...
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bioRxiv - Molecular Biology 2023Quote: ... 4sU-RNA was then biotinylated using 0.1 mg/ml MTSEA biotin-XX linker (Biotium, #BT90066) for 30 min at room temperature in the dark with rotation ...
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bioRxiv - Molecular Biology 2020Quote: ... 4tU-labeled RNA was biotinylated with 1 mg/mL MTSEA Biotin-XX (Biotium, Fremont, CA, #90066) for 30 min at room temperature in biotinylation buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... poly(A) RNA was biotinylated with activated disulfide methane thiosulfonate (MTS) biotin (Biotium Biotin-XX MTSEA). 4tU-containing RNA was enriched as previously described (Duffy et al ...
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bioRxiv - Biochemistry 2023Quote: ... 40 μg of RNA was biotinylated with 4 μg of MTSEA Biotin XX (Biotium Cat# 90066). The biotinylated RNA was isolated by binding to 80 μl of a Dynabeads MyOne Streptavidin C1 bead suspension (Invitrogen Cat# 65001 ...
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bioRxiv - Immunology 2021Quote: ... the following reagents were used: NucView Caspase-3 enzyme substrates (Biotium), Idelalisib (Selleckchem) ...
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bioRxiv - Microbiology 2022Quote: ... NucView 488 fluorescent caspase-3/7 substrate (Biotium, Fremont, CA, USA) was added at a final concentration of 1.25µg/ml to visualize cells undergoing apoptosis ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1:1000 NucView 488 Caspase-3/7 substrate (Biotium, 30029). Cells were treated with 10 µL of drug-containing media ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was denatured at 60°C for 10 min and biotinylated using 5 µg/mL biotin-MTS (Biotium) in 20% dimethylformamide (Sigma) ...
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bioRxiv - Biophysics 2023Quote: ... After removal of excess NeutrAvidin the flow cell is incubated with 150 pM biotinylated NTA beads (#90074, Biotium) for 3 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... fluorescent caspase 3/7 substrate for detecting apoptosis by live staining (Biotium) at 2 µM was prepared with dosing medium (M199 +2% v/v FBS) ...
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bioRxiv - Molecular Biology 2021Quote: RNA was biotinylated using 40 μl (~40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium #90066-1) in the following reaction ...
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bioRxiv - Cell Biology 2023Quote: ... Cells that were imaged were labeled using CF640-conjugated streptavidin to label biotinylated proteins (CF640R Streptavidin, Biotium cat 29041). Affinity purification was performed using streptavidin-conjugated magnetic beads (Pierce ...
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bioRxiv - Cancer Biology 2019Quote: ... Caspase activation was visualised using 1µM NucView 488 Caspase-3 Enzyme Substrate (Biotium). Cell viability was quantified using propidium iodide (1 μg/mL) ...
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bioRxiv - Immunology 2020Quote: ... sialic acid residues at the cell surface were oxidized using sodium meta-periodate (Thermo) and biotinylated with aminooxy-biotin (Biotium). Following quenching ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA was denatured at 60C for ten minutes before being biotinylated by the addition of 5ug/ml biotin-MTS (Biotium) in 20% dimethylformamide (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated with 5 μmol/L NucView™ 488 caspase-3 substrate (Biotium). Images were obtained in phase contrast and fluorescence mode using a x10 objective and an IncuCyte FLR time-lapse fluorescence microscope (Essen Bioscience) ...
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bioRxiv - Cell Biology 2022Quote: ... Beads were removed by centrifugation (3000 x g for 1 minute) and lysates split equally and incubated with either methanol (vehicle) or 1 μM biotinylated phalloidin (Biotium, 00028) for 2 hour at 4°C on a rotator ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were replaced with medium containing 5 µM NucView 488 caspase 3 substrate (Biotium Inc., #10403). After 30 min incubation at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Pink/Red-Gal substrate for B-gal activity detection (lineage tracing) was purchased from Biotium (10012).
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bioRxiv - Developmental Biology 2020Quote: ... NucView Caspase-3 substrate detection (green or red label to complement fluorescent hemocyte reporters) was performed according to the manufacturer’s instructions (Biotium); cells were incubated for 1 hour at room temperature ...
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bioRxiv - Immunology 2019Quote: ... Apoptosis assays were performed using Nucleocounter NC-3000 and a Flexicyte apoptosis/necrosis detection kit based on staining with caspase 3 substrate NucView 488 and RedDot 2 (all Biotium, Cambridge Bioscience, UK). Apoptotic cells are defined as NucView488 positive and RedDot2 negative ...