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Citations for Biotium Inc. :
1 - 42 of 42 citations for RT qPCR Master Mixes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... The amplification buffer and enzyme mixes provided in library kit was used for the master mix together with EvaGreen for qPCR (Biotium).
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bioRxiv - Immunology 2023Quote: ... qPCR was performed with the Fast EvaGreen qPCR Master Mix (Biotium) and measured on a ABI7300 Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... the Fast EvaGreen qPCR Master Mix (Biotium), and a ABI7300 Real-Time PCR System ...
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bioRxiv - Neuroscience 2023Quote: ... Copy number qPCR was performed using Forget-Me-Not qPCR Master Mix (Biotium) on a QuantStudio5 system.
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bioRxiv - Microbiology 2023Quote: ... using Fast Plus EvaGreen qPCR master mix (Biotium). Serial dilutions of 106-101 copies per reaction of MCMV genome containing BAC (clone pSM3fr-MCK-2fl 3.3 [51] ...
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bioRxiv - Microbiology 2023Quote: ... using Fast Plus EvaGreen qPCR master mix (Biotium). Cycling conditions were as follows ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting cDNA was diluted five-fold and RT-qPCR was performed with Eva™ Green PCR Master Mix (Biotium, Fremont, CA) in a StepOne Plus Real-Time PCR Platform (Applied Biosystems ...
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bioRxiv - Genetics 2021Quote: ... Quantitative PCR (qPCR) was preformed using the Forget-Me-Not qPCR Master Mix (Biotium) with a BioRad iCycler thermocycler ...
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bioRxiv - Genomics 2020Quote: ... qPCR reactions were performed using the Forget-Me-Not™ qPCR Master Mix (Biotium) in 20 μL volumes containing 1 X Forget-Me-Not™ qPCR Master Mix ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR was performed using Fast Plus EvaGreen® qPCR master mix High ROX (Biotium). Primers for genes of interest and genes of reference (GAPDH and HPRT1 ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 μL Fast Evagreen qPCR Master Mix (Biotium, 31003), 2 μL of nuclease-free water ...
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bioRxiv - Neuroscience 2023Quote: ... qPCR was performed using Forget-Me-Not™ EvaGreen® qPCR Master Mix (Biotium, USA). Data are presented as relative mRNA levels of the gene of interest normalized to GAPDH mRNA levels ...
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bioRxiv - Neuroscience 2022Quote: ... qPCR was carried out using Forget-Me-Not™ EvaGreen® qPCR Master Mix (Biotium; 31041-1) in the QuantStudio 7 Flex Real-Time PCR System (Fisher) ...
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bioRxiv - Developmental Biology 2021Quote: ... and qPCR was performed using Eva Green PCR Master Mix (Biotium) from nine regenerating fragments in four (Figure 4.2 ...
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bioRxiv - Genetics 2021Quote: ... using Forget-Me-Not EvaGreen qPCR Master Mix with low ROX (Biotium). Data quantification was performed using the mak3i module of the qpcR software library (version 1.4-0 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... qPCR assays were run with Forget-Me-Not Master Mix (Biotium 31044) in an Eppendorf Realplex 2 or BioRad CFX96 machine ...
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bioRxiv - Genetics 2019Quote: ... quantitative PCR (RT-qPCR) was performed on cDNA (described above) using Eva Green (Biotium) on a Step-One Plus Real time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... Each qPCR reaction contained 10μL of Master Mix EvaGreen 2X (Biotium, San Francisco, US), 1μM of each primer ...
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bioRxiv - Microbiology 2023Quote: ... and used in a qPCR using a ready-to-use mix containing a fluorescent-intercalating dye (EvaGreen® Fast qPCR Master Mix, Biotium, San Francisco, USA). After the amplification steps ...
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bioRxiv - Microbiology 2020Quote: ... The 10 µL qPCR reactions contained 1 µL of sample and 9 µL of master mix (0.25 mM of each primer, 1X Forget-Me-Not EvaGreen qPCR mix (Biotium, Fremont, CA), and 0.4 mg mL-1 BSA ...
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bioRxiv - Synthetic Biology 2019Quote: ... The primers (Microsynth, Balgach) were used in the Real-Time PCR reaction with Fast EvaGreen® qPCR Master Mix (Biotium, USA) on ABI StepOnePlus (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... 1 μl of gDNA was used as template in a 20 μl qPCR reaction containing 10 μl of 2x Fast-Plus EvaGreen Master Mix with low ROX dye (Biotium, Inc. via VWR, Germany), 10 pmol of each forward and reverse primer (Table 1) ...
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bioRxiv - Genomics 2024Quote: ... 1X EvaGreen qPCR dye (Biotium 31000), 1M Betaine (MilliporeSigma B0300) ...
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bioRxiv - Genomics 2020Quote: qPCR (Q5 UltraII mastermix with EvaGreen (Biotium), Ta=66 ...
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bioRxiv - Genomics 2020Quote: ... and qPCR were from Biotium (Fremont, CA). Oligonucleotides were obtained from IDT (Coralville ...
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bioRxiv - Bioengineering 2021Quote: ... Eva Green Master Mix (Biotium, Fremont, CA) was used with custom-made forward and reverse primers (Table S1 ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 µl 5x HOT FIREPol EvaGreen qPCR Supermix (Biotium), 0.5 µl forward (10 µM stock ...
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bioRxiv - Systems Biology 2020Quote: ... added to a qPCR mix (0.275 μL EvaGreen [31000, Biotium, Fremont ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed using EvaGreen (Biotium, Catalog no: 89138-982) and the Biorad CFX Connect Real-time PCR system ...
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bioRxiv - Genomics 2024Quote: ... We used Fast Probe Master Mix (Biotium Inc. Fremont, CA) and followed the recommended preparation protocol (Standard Biotools 2015b) ...
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bioRxiv - Systems Biology 2020Quote: ... we ran a qPCR by adding 2.5 μL of 20x EvaGreen (Biotium) to one replica sample to visualize the plateau phase ...
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bioRxiv - Molecular Biology 2019Quote: ... The qPCR was performed using EvaGreen Real-time PCR (Biotium, Hayward, USA) according to the manufacturer’s recommendations using 1µl EvaGreen Real-time PCR (Biotium ...
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bioRxiv - Microbiology 2020Quote: ... 2X Forget-Me-Not Universal Probe Master Mix with ROX (Biotium 31044-1) was used to perform Q-RT-PCR in an Applied Biosystems StepOnePlus™ Real-Time PCR System Thermal Cycling Block ...
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bioRxiv - Cell Biology 2023Quote: ... The number of cycles for each PCR reaction was optimized by qPCR with EvaGreen (Biotium, 31000) on a CFX Opus 96 Real-Time PCR System (Bio-Rad).
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bioRxiv - Genomics 2023Quote: ... rinsed in PBS for 10 min at RT and blocked using blocking buffer (Fish Gelatin blocking agent, BIOTIUM) in the dark for 1 hr ...
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bioRxiv - Microbiology 2022Quote: ... The viability of the bacteria was determined using qPCR after PMAxx™ Dye treatment (Biotium San Francisco, US) allowing specific DNA amplification of viable bacteria only ...
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bioRxiv - Microbiology 2020Quote: ... and the RT-PCR products were analysed by standard agarose gel electrophoresis visualized by GelRed Nucleic Acid Stain (Biotium) and subjected to Sanger sequencing (core facility of the Haartman Institute ...
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bioRxiv - Genetics 2020Quote: To test the relative expression of XIST across cell lines quantitative real-time PCR (qPCR) was carried out using EVAgreen (Biotium) and Maxima Hot Start Taq (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: To differentiate between viable and non-viable cells and viruses in qPCR, half of the aliquots mentioned above (i.e., the second set of triplicates) received a PMAxx dye treatment (Biotium, Fremont, CA). Aliquots of the biomass samples (230 μL ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were allowed to cure protected from light at RT for at least one day before the short edges of the coverglass only were sealed with CoverGrip (Biotium #23005, Fremont, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... 5 minutes each with PBS and incubated for 10 minutes at RT with a 1:200 dilution in PBS of the lipophilic dye CellBrite™ (Biotium. Cat. Nº: 30023). Finally ...
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bioRxiv - Microbiology 2023Quote: ... Real-time PCR reactions were then performed to determine the mRNA levels of target genes or DNA levels using RT2 Forget-Me-NotTM EvaGreen qPCR Mastermix (Biotium, Assay Matrix, Melbourne, Australia) on a CFX Connect Real Time PCR System (BioRad) ...