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Citations for Biotium Inc. :
1 - 50 of 134 citations for RNA Amplification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... in Tyramide Amplification Buffer Plus (Biotium, 22029) as described by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated for 1 h at RT with the secondary antibodies HRP goat anti-mouse (1:200 in 1% BSA-PBS) followed by 10 min amplification reaction with tyramide for the detection of Ngn3 (Tyramide Amplification Kid, 33003, Biotium, USA), or Alexa 594 donkey anti-rabbit for the detection of Pomc/Npy (1:1000 in 1% BSA-PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were washed in PBS and Tyramide signal amplification (Biotium) was performed according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR amplification was verified by visualizing on a 1 % agarose gel with GelRed (Biotium) and 1 kb Hyperladder (Bioline) ...
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bioRxiv - Microbiology 2023Quote: ... DNA amplification was observed by electrophoresis in agarose 1% gel stained with GelRed® (Biotium, USA). The DNA from T ...
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bioRxiv - Genetics 2024Quote: ... Amplification success was verified on a 0.8% agarose gel stained with GelRed Nucleic Acid Gel (Biotium). After verification ...
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bioRxiv - Genomics 2020Quote: Antibodies were detected using either the tyramide signal amplification (TSA) (33002 CFF488A Streptavidin HRP, Biotium, Fremont, CA) and streptavidin-conjugated Alexa-fluor 488 (1 ...
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bioRxiv - Developmental Biology 2023Quote: ... we used the Tyramide signal amplification (TSA) detection system (PerkinElmer, MA, USA, or Biotium, Inc. CA, USA) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The success of amplification was monitored by 10% polyacrylamide gel electrophoresis and staining with GelGreen DNA dye (Biotium).
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bioRxiv - Evolutionary Biology 2019Quote: ... Amplification success and product length were confirmed visually using a 1.2% agarose gel post-stained with GelRed (Biotium Inc.). Successful amplification products were then purified using an ExoSAP-IT PCR clean-up kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The amplification products were visualized by electrophoresis on a 0.8 % agarose gel stained with Gel Red (Biotium, Hayward, USA) at 80 V.
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bioRxiv - Cancer Biology 2023Quote: ... Amplification products were visualised by electrophoresis on 1.5% w/v agarose gel and visualised by Gel Red (Biotium, Cat. No. 41002) using manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Amplification products were visualized by gel electrophoresis on 2% agarose gel stained with GelRed® Nucleic Acid Gel Stain (Biotium Inc., Fremont, CA, USA).
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bioRxiv - Molecular Biology 2021Quote: RNA was biotinylated using 40 μl (~40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium #90066-1) in the following reaction ...
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bioRxiv - Microbiology 2019Quote: ... RNA was visualised using EvaGreen fluorescent nucleic acid dye (Biotium) on a ChemiDoc MP imager (Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... 70 μg of RNA was biotinylated with MTSEA biotin-XX (Biotium) as previously described (Chan et al. ...
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bioRxiv - Immunology 2019Quote: ... using an AccuBlue Broad Range kit (Biotium). Then ...
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bioRxiv - Neuroscience 2022Quote: ... Cellbrite blue (Cytoplasmic Membrane-Labeling Kit - Biotium). Craniotomy and skull surface were submerged with aCSF (see Experimental procedures ...
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bioRxiv - Cell Biology 2019Quote: ... the Firefly Luciferase Assay Kit from Biotium was used according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Steady-Luc HTS Assay Kit from Biotium was used for the luciferase assays ...
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bioRxiv - Molecular Biology 2020Quote: ... and the remaining 4sU-labeled nascent RNA was biotinylated using MTSEA-Biotin-XX (Biotium, 90066) as previously described67,81 ...
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bioRxiv - Molecular Biology 2019Quote: ... 2016) using 40 μl (∼40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium) in the following reaction ...
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bioRxiv - Microbiology 2020Quote: ... and the remaining 4sU-labeled nascent RNA was biotinylated using MTSEA-Biotin-XX (Biotium; 90066) as previously described83 ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was biotin-labelled with 50 μl 0.1 mg/ml MTSEA biotin-XX linker (Biotium) with 3 μl biotin buffer (833 mM Tris HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... 2015) using 40 μl (∼40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium) in the following reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... 4sU-RNA was then biotinylated using 0.1 mg/ml MTSEA biotin-XX linker (Biotium, #BT90066) for 30 min at room temperature in the dark with rotation ...
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bioRxiv - Genomics 2023Quote: ... 64 using 40 μl (∼40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium) in the following reaction ...
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bioRxiv - Cell Biology 2020Quote: ... antibody labeling kit Mix-n-Stain CF640R (Biotium); heme (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... or CF594 TUNEL Assay Apoptosis Detection Kit (Biotium) according to the manufacturers’ protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... 4tU-labeled RNA was biotinylated with 1 mg/mL MTSEA Biotin-XX (Biotium, Fremont, CA, #90066) for 30 min at room temperature in biotinylation buffer (20 mM HEPES ...
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bioRxiv - Neuroscience 2022Quote: ... The integrity of the total RNA was assessed using agarose gel stained with GelRed™ (Biotium) and spectrophotometric analysis using NanoDrop 2000/2000c ...
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bioRxiv - Molecular Biology 2022Quote: ... poly(A) RNA was biotinylated with activated disulfide methane thiosulfonate (MTS) biotin (Biotium Biotin-XX MTSEA). 4tU-containing RNA was enriched as previously described (Duffy et al ...
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bioRxiv - Molecular Biology 2023Quote: ... Isolated RNA was separated on a 1% agarose gel (1XTBE) and visualized using GelRed dye (Biotium). Both inputs (corresponds to 5% ...
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bioRxiv - Biochemistry 2023Quote: ... 40 μg of RNA was biotinylated with 4 μg of MTSEA Biotin XX (Biotium Cat# 90066). The biotinylated RNA was isolated by binding to 80 μl of a Dynabeads MyOne Streptavidin C1 bead suspension (Invitrogen Cat# 65001 ...
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bioRxiv - Molecular Biology 2023Quote: ... using the AccuGreen Broad Range dsDNA quantification kit (Biotium). Samples were diluted at the concentration of 2.5 ng/μl ...
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bioRxiv - Microbiology 2023Quote: ... or Resazurin Cell Viability Assay Kit (Biotium #30025-1). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... Viafluor-488 live cell microtubule staining kit (Biotium, #70062) were used for live imaging of mNPCs in vitro.
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bioRxiv - Biochemistry 2022Quote: ... RNA integrity was evaluated by electrophoresis on 1 % agarose gel using the GelGreen Nucleic Acid Stain (Biotium) dye following the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... and quantified using an Accuclear DNA Kit (Biotium, Fremont, USA), 75bp PE reads were generated on the Illumina HiSeq 2500.
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bioRxiv - Molecular Biology 2023Quote: ... RNA was denatured at 60°C for 10 min and biotinylated using 5 µg/mL biotin-MTS (Biotium) in 20% dimethylformamide (Sigma) ...
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bioRxiv - Microbiology 2024Quote: 5 mg of RNA was incubated with 2.5 mg biotin-conjugated MTSEA (1 mg/ml) (Biotium 90066-1) in MTSEA buffer [20 mM HEPES pH 7.3 (Quality Biological A611-J848-10) ...
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bioRxiv - Cancer Biology 2020Quote: NucView 488/ MitoView 633 apoptosis assay kit (Biotium, Fremont, United States) was used to determine apoptotic cell rate according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... and the assay was performed using the Griess Reagent Kit (Biotium) following the guidelines as provided in the kit ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA was denatured at 60C for ten minutes before being biotinylated by the addition of 5ug/ml biotin-MTS (Biotium) in 20% dimethylformamide (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... luciferase activity was measured using a luciferase assay kit (Biotium, Hayward, CA) with a microplate reader (VictorTM X5 ...
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bioRxiv - Microbiology 2022Quote: ... Luciferase activity was determined using either the Renilla Luciferase Assay Kit (Biotium), Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Bioengineering 2022Quote: Rat RVLM sections were stained using a TUNEL kit (Biotium, Fremont, CA) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2021Quote: The JC-1 mitochondrial membrane potential detection kit (Biotium, Hayward, CA, USA) was used for measuring mitochondrial membrane potential in oocytes ...
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bioRxiv - Bioengineering 2023Quote: ... the Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Biotium, UK) was used according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... with either AccuGreen broad-range or high-sensitivity dsDNA detection kits (Biotium). To preserve mutant abundance ratios and reduce the propagation of PCR errors ...