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Citations for Biotium Inc. :
1 - 50 of 488 citations for 7 Fluoro 6 amino 2H 1 4 benzoxazin 3 4H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and 1:1000 NucView 488 Caspase-3/7 substrate (Biotium, 30029). Cells were treated with 10 µL of drug-containing media ...
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bioRxiv - Immunology 2023Quote: ... Dead cells and doublets were excluded from analyses based on staining of 7-amino-actinomycin D (7-AAD) (Biotium) or Zombie yellow (catalog ...
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bioRxiv - Neuroscience 2022Quote: ... and Alexa fluoro-568 conjugated a-bungarotoxin (1:500, Biotium 00006) for 3 hours at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and Alexa fluoro-568 conjugated α-bungarotoxin (1:500, Biotium 00006) at room temperature for 3 hours ...
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bioRxiv - Biochemistry 2023Quote: ... 50 μL of 1 mg/mL XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) (Biotium) was mixed with 5 μL of 1.5 mg/mL phenazine methosulfate ...
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bioRxiv - Immunology 2020Quote: Cell death within tumor slices was assessed using a green-fluorescent caspase 3/7 probe that binds DNA upon cleavage by caspase 3/7 (Nucview, Biotium). 2.106 untransduced or EGFR CAR T cells were applied onto BxPC3 tumor slices that were subsequently incubated with 5μM of NucView dye ...
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bioRxiv - Systems Biology 2021Quote: Genomic DNA was quantified using Sybr Green fluorescence assay with a 6-point DNA standard curve (0, 0.5, 1, 2, 4, 6 ng/μL Biotium). 1 μL of samples and 5 μL of standards were diluted into 95 μL of 1X SYBR Green (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... NucView 488 fluorescent caspase-3/7 substrate (Biotium, Fremont, CA, USA) was added at a final concentration of 1.25µg/ml to visualize cells undergoing apoptosis ...
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bioRxiv - Cancer Biology 2023Quote: ... then were incubated for 10 minutes at 21 °C in 1 μM 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM, Biotium) in PSS ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product by mitochondrial dehydrogenase enzymes ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
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bioRxiv - Bioengineering 2023Quote: The percent toxicity was calculated using an XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) colorimetric assay (Biotium) wherein viable cells form an orange formazan product upon reduction ...
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bioRxiv - Cancer Biology 2021Quote: ... fluorescent caspase 3/7 substrate for detecting apoptosis by live staining (Biotium) at 2 µM was prepared with dosing medium (M199 +2% v/v FBS) ...
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bioRxiv - Plant Biology 2021Quote: ... 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) from Biotium (CA, USA), Wortmannin (WM ...
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bioRxiv - Bioengineering 2024Quote: Cell proliferation was evaluated using the XTT (2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay (Biotium Inc., Fremont, CA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... containing 0.5 μg/ml DAPI (4’,6-diamidino-2-phenylindole, Biotium). Images were acquired on an LSM 700 confocal microscope (Zeiss ...
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bioRxiv - Cancer Biology 2020Quote: ... and 7 using CalceinAM (Biotium, CA; 148504-34-1), Ethidium Homodimer I (Biotium ...
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bioRxiv - Cell Biology 2023Quote: ... Wash Buffer with 1 mM ADVASEP-7 (Biotium; 70029), and finally Wash Buffer alone ...
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bioRxiv - Cell Biology 2021Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (10µg/ml, Biotium, cat. number 40043) was used for nuclear staining ...
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bioRxiv - Cell Biology 2023Quote: ... After nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI) (40043, Biotium) in IF buffer at a final concentration of 0.5 µg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... After nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI) (40043, Biotium) in IF buffer at a final concentration of µg/ml ...
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bioRxiv - Genetics 2021Quote: ... 40 μl of 1X 4’,6-diamidino-2-phenylindole (DAPI; Biotium; Reagent Table) was added and the slides were sealed with a coverslip and clear nail polish.
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bioRxiv - Biochemistry 2021Quote: ... The pHi of ArchT transfected RPE cells and control RPE cells were directly calculated using 2’,7’-bis(carboxyethyl)-5(6)-Carboxyfluorescein (BCECF, Biotium). BCECF is a ratiometric dual excitation/single-emission pH biosensor (405 ex/525 em for pH insensitive fluorescence ...
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bioRxiv - Neuroscience 2022Quote: ... ADVASEP-7 (Biotium) was also resuspended at 500 μM in HBSS ...
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bioRxiv - Neuroscience 2024Quote: ... and DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate, Biotium, 60014-10mg) were dissolved in 100% ethanol (Koptec ...
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bioRxiv - Neuroscience 2023Quote: ... Biocytin (1-3 mg/mL, Biotium) was dissolved in the pipette solution for post-hoc identification of recorded cells.
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bioRxiv - Cell Biology 2023Quote: ... PCR products were run on a 1.5-2% agarose gel (7-01P02-R, BioConcept) in TAE buffer (3-07F03-I, BioConcept) and imaged using GelRed Nucleic Acid Stain (41003, Biotium). For RT-qPCR ...
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bioRxiv - Genomics 2022Quote: ... 7-AAD (Biotium, 40037) was stained to exclude dead cells ...
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bioRxiv - Neuroscience 2020Quote: ... Neuro-DiI (7% in ethanol; Biotium) was slowly injected ...
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bioRxiv - Neuroscience 2020Quote: ... Neuro- DiI (7% in ethanol; Biotium) was slowly injected ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then washed with PHEM-T and counter-stained with 4′,6-diamidino-2-phenylindole (DAPI, Biotium, Fremont, CA). The cells and coverslips were mounted on glass slides with Prolong Glass Antifade Mountant (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were mounted using EverBrite mounting medium with 4′,6-diamidino-2-phenylindole (Cat No. 23002, Biotium, Fremont, CA, USA). Images were recorded by Olympus IX51 microscope with ToupTek camera and with the same exposure parameters (Alexa Fluor™ 555 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed three times to remove secondary antibody and were then stained with 4’,6-diamindino-2-phenylindole (DAPI; Biotium, 40011) diluted in PBS for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... the IVT reaction was doped with 5-amino-allyl UTP (Biotium) at 1:10 (aminoallyl-UTP ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was then amplified in the presence of 0.225mM amino-allyl-dUTP (Biotium) using Taq DNA polymerase (New England Biolabs) ...
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bioRxiv - Neuroscience 2021Quote: ... we incubated one cochlea in HBSS containing annexin V-Alexa647 at a 1/25 dilution and CF488A-WGA (Biotium) at 20 μg/mL and the other cochlea in the same buffer supplemented with 100 μM benzamil for 25 min at RT in an orbital shaker protected from the light ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with 1 μM calcein-AM (Biotium, cat no. 80011-3) for 10 mins ...
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bioRxiv - Cell Biology 2021Quote: ... fluo-3 acetoxymethyl (Fluo-3 thereafter) ester (Biotium, US) following a protocol adapted from (Zhang et al. ...
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bioRxiv - Molecular Biology 2023Quote: The free radical probe 2’,7’-dichlorodihydrofluoresceindiacetate (H2DCFDA) (Biotium, Fremont, CA) was used to quantify ROS ...
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bioRxiv - Biochemistry 2024Quote: ... The gel was then stained with One-Step Blue (Biotium) for one hour and then de-stained in Mili-Q water overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated at 4°C for 4 h with CF488-conjugated anti-mouse IgG (1:2000) and CF568-conjugated anti-rabbit IgG (1:2000) antibodies (Biotium) diluted in PBS containing 2% FBS as secondary antibodies ...
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bioRxiv - Immunology 2021Quote: ... stained with one-step blue protein gel stain (Biotium, ref. 21003), and by SEC-HPLC with an xbridge protein BEH SEC (Waters ...
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bioRxiv - Cell Biology 2021Quote: ... and 4 uL of 1 mM of SGC5 (Cat. number 70057, Biotium Inc., Fremont, CA) was injected into the AC of an enucleated mouse eye ...
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bioRxiv - Neuroscience 2019Quote: ... which we measured in the presence of 10−7 M tetrodotoxin (TTX; Biotium). TTX blocks sodium channels and therefore removes all action potential activity ...
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bioRxiv - Developmental Biology 2022Quote: ... qRT-PCR was run on a ViiA 7 with SYBR green mastermix (Biotium). 18S was used as the human cell housekeeping gene and Ywhaz was used as the mouse housekeeping gene ...
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bioRxiv - Cancer Biology 2021Quote: ... Activity of caspase-3 was detected with NucView 488 Caspase-3 Assay kit (Biotium) according to the instructions of the manufacturer.
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bioRxiv - Cell Biology 2023Quote: ... loaded with membrane-permeant AM ester forms of Fluo-3 (Fluo-3-AM) (Biotium) and/or Fura-Red ...
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bioRxiv - Cell Biology 2021Quote: ... or Proteostat (3 μM, Biotium) solutions.
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bioRxiv - Biochemistry 2024Quote: ... stained with 3 × GelRed (Biotium) for 30 min and visualized by using Chemidoc™ (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... 6-diamidino-2-phenylindole (DAPI) was purchased from Biotium, CA ...