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Citations for Biotium Inc. :
1 - 50 of 211 citations for 7 16 DIHYDROBENZO A BENZO 5 6 QUINO 3 2 I ACRIDINE 9 18 DIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) from Biotium (CA, USA), Wortmannin (WM ...
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bioRxiv - Cancer Biology 2023Quote: ... then were incubated for 10 minutes at 21 °C in 1 μM 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM, Biotium) in PSS ...
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bioRxiv - Biochemistry 2021Quote: ... The pHi of ArchT transfected RPE cells and control RPE cells were directly calculated using 2’,7’-bis(carboxyethyl)-5(6)-Carboxyfluorescein (BCECF, Biotium). BCECF is a ratiometric dual excitation/single-emission pH biosensor (405 ex/525 em for pH insensitive fluorescence ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were run on a 1.5-2% agarose gel (7-01P02-R, BioConcept) in TAE buffer (3-07F03-I, BioConcept) and imaged using GelRed Nucleic Acid Stain (41003, Biotium). For RT-qPCR ...
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bioRxiv - Immunology 2020Quote: Cell death within tumor slices was assessed using a green-fluorescent caspase 3/7 probe that binds DNA upon cleavage by caspase 3/7 (Nucview, Biotium). 2.106 untransduced or EGFR CAR T cells were applied onto BxPC3 tumor slices that were subsequently incubated with 5μM of NucView dye ...
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bioRxiv - Cancer Biology 2020Quote: ... Ethidium Homodimer I (Biotium; 61926-22-5) per manufacturer protocol and expression of phenotypical markers was measured at day 5 via immunostaining ...
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bioRxiv - Microbiology 2022Quote: ... NucView 488 fluorescent caspase-3/7 substrate (Biotium, Fremont, CA, USA) was added at a final concentration of 1.25µg/ml to visualize cells undergoing apoptosis ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1:1000 NucView 488 Caspase-3/7 substrate (Biotium, 30029). Cells were treated with 10 µL of drug-containing media ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... The larvae were housed in 10 μM INK128 (or DMSO) containing water and at 36 hpa they were treated with 5 μM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Biotium, #10058) for 55 min ...
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bioRxiv - Cancer Biology 2021Quote: ... fluorescent caspase 3/7 substrate for detecting apoptosis by live staining (Biotium) at 2 µM was prepared with dosing medium (M199 +2% v/v FBS) ...
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bioRxiv - Molecular Biology 2023Quote: The free radical probe 2’,7’-dichlorodihydrofluoresceindiacetate (H2DCFDA) (Biotium, Fremont, CA) was used to quantify ROS ...
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bioRxiv - Biochemistry 2020Quote: ... 6-diamidino-2-phenylindole (DAPI) was purchased from Biotium, CA ...
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bioRxiv - Microbiology 2021Quote: ... containing 0.5 μg/ml DAPI (4’,6-diamidino-2-phenylindole, Biotium). Images were acquired on an LSM 700 confocal microscope (Zeiss ...
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bioRxiv - Systems Biology 2021Quote: Genomic DNA was quantified using Sybr Green fluorescence assay with a 6-point DNA standard curve (0, 0.5, 1, 2, 4, 6 ng/μL Biotium). 1 μL of samples and 5 μL of standards were diluted into 95 μL of 1X SYBR Green (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (10µg/ml, Biotium, cat. number 40043) was used for nuclear staining ...
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bioRxiv - Cell Biology 2023Quote: ... After nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI) (40043, Biotium) in IF buffer at a final concentration of 0.5 µg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... After nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI) (40043, Biotium) in IF buffer at a final concentration of µg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... ADVASEP-7 (Biotium) was also resuspended at 500 μM in HBSS ...
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bioRxiv - Genetics 2021Quote: ... 40 μl of 1X 4’,6-diamidino-2-phenylindole (DAPI; Biotium; Reagent Table) was added and the slides were sealed with a coverslip and clear nail polish.
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bioRxiv - Genomics 2022Quote: ... 7-AAD (Biotium, 40037) was stained to exclude dead cells ...
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bioRxiv - Biophysics 2021Quote: ... The upstream handle was labeled at the 3’ end of the DNA strand by introducing biotin-16-dUTP (Biotium, Fremont, CA, USA) using the T4 DNA polymerase (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... 16 xg at 4°C and the pellet resuspended and incubated in WGA488 stain (Biotium, 5 μg/mL, diluted in HBSS) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were separated on a 1.5-2% agarose gel (7-01P02-R, BioConcept) dissolved in TAE buffer (3-07F03-I, BioConcept) and visualized using GelRed Nucleic Acid Stain (Cat# 41003, Biotium). Presence of rat cells in myoblast culture/muscle lysate was assessed by PCR for rat dystrophin using primers Rat Dmd i22-i23.F/ Rat Dmd i22-i23.R and GoTaq G2 Hot Start Green Master Mix (M7423 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated with 5 μmol/L NucView™ 488 caspase-3 substrate (Biotium). Images were obtained in phase contrast and fluorescence mode using a x10 objective and an IncuCyte FLR time-lapse fluorescence microscope (Essen Bioscience) ...
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bioRxiv - Bioengineering 2023Quote: The percent toxicity was calculated using an XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) colorimetric assay (Biotium) wherein viable cells form an orange formazan product upon reduction ...
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bioRxiv - Biochemistry 2023Quote: ... 50 μL of 1 mg/mL XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) (Biotium) was mixed with 5 μL of 1.5 mg/mL phenazine methosulfate ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed with PBS (3 × 5 min) and treated with Trueblack (Biotium, Cat# 23007) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Neuro-DiI (7% in ethanol; Biotium) was slowly injected ...
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bioRxiv - Neuroscience 2020Quote: ... Neuro- DiI (7% in ethanol; Biotium) was slowly injected ...
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bioRxiv - Bioengineering 2023Quote: ... Ethidium homodimer I (Biotium), and Hoechst 33342 (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were replaced with medium containing 5 µM NucView 488 caspase 3 substrate (Biotium Inc., #10403). After 30 min incubation at room temperature ...
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bioRxiv - Bioengineering 2024Quote: Cell proliferation was evaluated using the XTT (2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay (Biotium Inc., Fremont, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Dead cells and doublets were excluded from analyses based on staining of 7-amino-actinomycin D (7-AAD) (Biotium) or Zombie yellow (catalog ...
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bioRxiv - Microbiology 2022Quote: ... samples were placed on a microscope slide with 0.8% agarose gel containing 5 μM Bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC4(3)) (Biotium) and 0.75 μM permeability reporter PI (MP Biomedicals) ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then washed with PHEM-T and counter-stained with 4′,6-diamidino-2-phenylindole (DAPI, Biotium, Fremont, CA). The cells and coverslips were mounted on glass slides with Prolong Glass Antifade Mountant (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were mounted using EverBrite mounting medium with 4′,6-diamidino-2-phenylindole (Cat No. 23002, Biotium, Fremont, CA, USA). Images were recorded by Olympus IX51 microscope with ToupTek camera and with the same exposure parameters (Alexa Fluor™ 555 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed three times to remove secondary antibody and were then stained with 4’,6-diamindino-2-phenylindole (DAPI; Biotium, 40011) diluted in PBS for 20 minutes at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... and 7 using CalceinAM (Biotium, CA; 148504-34-1), Ethidium Homodimer I (Biotium ...
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bioRxiv - Cell Biology 2023Quote: ... Wash Buffer with 1 mM ADVASEP-7 (Biotium; 70029), and finally Wash Buffer alone ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested on day 5 and the proportion of apoptotic cells was assessed using the NucView 488 Caspase-3 Assay Kit (Biotium) according to the manufacturer’s instructions and an Attune NxT flow cytometer (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... The cell viability was documented via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Biotium, Fremont, CA).
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bioRxiv - Cell Biology 2021Quote: ... fluo-3 acetoxymethyl (Fluo-3 thereafter) ester (Biotium, US) following a protocol adapted from (Zhang et al. ...
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bioRxiv - Immunology 2019Quote: ... Apoptosis assays were performed using Nucleocounter NC-3000 and a Flexicyte apoptosis/necrosis detection kit based on staining with caspase 3 substrate NucView 488 and RedDot 2 (all Biotium, Cambridge Bioscience, UK). Apoptotic cells are defined as NucView488 positive and RedDot2 negative ...
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bioRxiv - Neuroscience 2019Quote: ... which we measured in the presence of 10−7 M tetrodotoxin (TTX; Biotium). TTX blocks sodium channels and therefore removes all action potential activity ...
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bioRxiv - Developmental Biology 2022Quote: ... qRT-PCR was run on a ViiA 7 with SYBR green mastermix (Biotium). 18S was used as the human cell housekeeping gene and Ywhaz was used as the mouse housekeeping gene ...
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bioRxiv - Cancer Biology 2021Quote: ... Activity of caspase-3 was detected with NucView 488 Caspase-3 Assay kit (Biotium) according to the instructions of the manufacturer.
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bioRxiv - Cell Biology 2023Quote: ... loaded with membrane-permeant AM ester forms of Fluo-3 (Fluo-3-AM) (Biotium) and/or Fura-Red ...
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bioRxiv - Cell Biology 2021Quote: ... or Proteostat (3 μM, Biotium) solutions.
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bioRxiv - Biochemistry 2024Quote: ... stained with 3 × GelRed (Biotium) for 30 min and visualized by using Chemidoc™ (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Cleaned barcoded 16S rRNA amplicons were quantified using AccuClear Ultra High Sensitivity dsDNA Quantitation Kit (Biotium) on BioTek™ FLx800™ Microplate Fluorescence Reader ...