Labshake search
Citations for Biotium Inc. :
1 - 50 of 230 citations for 6 methyl 4 oxo 5 6 dihydro 4H thieno 2 3 b thiopyran 2 sulfonyl chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... containing 0.5 μg/ml DAPI (4’,6-diamidino-2-phenylindole, Biotium). Images were acquired on an LSM 700 confocal microscope (Zeiss ...
-
bioRxiv - Plant Biology 2021Quote: ... 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) from Biotium (CA, USA), Wortmannin (WM ...
-
bioRxiv - Cell Biology 2021Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (10µg/ml, Biotium, cat. number 40043) was used for nuclear staining ...
-
bioRxiv - Cell Biology 2023Quote: ... After nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI) (40043, Biotium) in IF buffer at a final concentration of 0.5 µg/ml ...
-
bioRxiv - Cell Biology 2023Quote: ... After nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI) (40043, Biotium) in IF buffer at a final concentration of µg/ml ...
-
bioRxiv - Systems Biology 2021Quote: Genomic DNA was quantified using Sybr Green fluorescence assay with a 6-point DNA standard curve (0, 0.5, 1, 2, 4, 6 ng/μL Biotium). 1 μL of samples and 5 μL of standards were diluted into 95 μL of 1X SYBR Green (Invitrogen ...
-
bioRxiv - Genetics 2021Quote: ... 40 μl of 1X 4’,6-diamidino-2-phenylindole (DAPI; Biotium; Reagent Table) was added and the slides were sealed with a coverslip and clear nail polish.
-
bioRxiv - Cancer Biology 2023Quote: ... then were incubated for 10 minutes at 21 °C in 1 μM 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM, Biotium) in PSS ...
-
bioRxiv - Biochemistry 2020Quote: ... 6-diamidino-2-phenylindole (DAPI) was purchased from Biotium, CA ...
-
bioRxiv - Biochemistry 2021Quote: ... The pHi of ArchT transfected RPE cells and control RPE cells were directly calculated using 2’,7’-bis(carboxyethyl)-5(6)-Carboxyfluorescein (BCECF, Biotium). BCECF is a ratiometric dual excitation/single-emission pH biosensor (405 ex/525 em for pH insensitive fluorescence ...
-
bioRxiv - Neuroscience 2020Quote: Intracellular chloride measurements were performed using the N-(Ethoxycarbonylmethyl)-6-Methoxyquinolinium Bromide (MQAE) (Biotium) chloride sensor.
-
bioRxiv - Microbiology 2022Quote: ... The cells were then washed with PHEM-T and counter-stained with 4′,6-diamidino-2-phenylindole (DAPI, Biotium, Fremont, CA). The cells and coverslips were mounted on glass slides with Prolong Glass Antifade Mountant (Thermo Scientific) ...
-
bioRxiv - Neuroscience 2023Quote: ... the cells were mounted using EverBrite mounting medium with 4′,6-diamidino-2-phenylindole (Cat No. 23002, Biotium, Fremont, CA, USA). Images were recorded by Olympus IX51 microscope with ToupTek camera and with the same exposure parameters (Alexa Fluor™ 555 ...
-
bioRxiv - Neuroscience 2023Quote: ... Slides were washed three times to remove secondary antibody and were then stained with 4’,6-diamindino-2-phenylindole (DAPI; Biotium, 40011) diluted in PBS for 20 minutes at room temperature ...
-
bioRxiv - Biophysics 2021Quote: ... Octadecyl rhodamine B chloride (R18) dye was purchased from Biotium, Inc ...
-
bioRxiv - Biochemistry 2023Quote: ... 50 μL of 1 mg/mL XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) (Biotium) was mixed with 5 μL of 1.5 mg/mL phenazine methosulfate ...
-
bioRxiv - Bioengineering 2023Quote: The percent toxicity was calculated using an XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) colorimetric assay (Biotium) wherein viable cells form an orange formazan product upon reduction ...
-
bioRxiv - Neuroscience 2022Quote: ... Cholera toxin subunit B CF-640 (Biotium, 2 mg/ml, 100 nl), AAV1-hSyn-Cre-WPRE-hGH (Addgene ...
-
bioRxiv - Bioengineering 2024Quote: Cell proliferation was evaluated using the XTT (2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay (Biotium Inc., Fremont, CA) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... 6 μL Fast Evagreen qPCR Master Mix (Biotium, 31003), 2 μL of nuclease-free water ...
-
bioRxiv - Microbiology 2024Quote: ... resazurin (Biotium #30025-2) was added as a 10% volume of sample wells or to 100 μL of media only (as a fluorescent background control) ...
-
bioRxiv - Neuroscience 2019Quote: ... then diluted to 6x) and run on agarose gels (1-2%) with 4% GelRed (Biotium, California, USA).
-
bioRxiv - Neuroscience 2023Quote: ... cells were pre-incubated with Fura-2·AM or Fluo-4·AM (30 minutes, 1 μM, Biotium) at 37°C with 5% CO2 ...
-
bioRxiv - Neuroscience 2023Quote: ... Cholera toxin subunit B (ctb)- 640 or -568 (5 mg/ml, Biotium) was injected in NAc medial shell and core (from bregma ...
-
bioRxiv - Bioengineering 2023Quote: ... The cell viability was documented via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Biotium, Fremont, CA).
-
bioRxiv - Biophysics 2019Quote: FM 4-64 (SynaptoRed™C2) and FM 2-10 (SynaptoGreen™C2) were purchased from Biotium (Fremont, CA) to use as membrane probes ...
-
bioRxiv - Cell Biology 2020Quote: ... 4 μl of the digested DNA was mixed with 2 μl 1:100 in DMSO diluted GelRed™ (Biotium) dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
-
bioRxiv - Microbiology 2020Quote: ... and ethidiumhomodimer-2 (Biotium, California, USA), at a concentration of 2 μM and 4 μM respectively and incubated for 30 min at 37°C ...
-
Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... The larvae were housed in 10 μM INK128 (or DMSO) containing water and at 36 hpa they were treated with 5 μM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Biotium, #10058) for 55 min ...
-
bioRxiv - Immunology 2019Quote: ... Apoptosis assays were performed using Nucleocounter NC-3000 and a Flexicyte apoptosis/necrosis detection kit based on staining with caspase 3 substrate NucView 488 and RedDot 2 (all Biotium, Cambridge Bioscience, UK). Apoptotic cells are defined as NucView488 positive and RedDot2 negative ...
-
bioRxiv - Neuroscience 2024Quote: ... and DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate, Biotium, 60014-10mg) were dissolved in 100% ethanol (Koptec ...
-
bioRxiv - Pathology 2021Quote: Lipofuscin present in the brain of 6 month-old mice was quenched with TrueBlackTM Lipofuscin Autofluorescence Quencher (Biotium). Briefly ...
-
bioRxiv - Microbiology 2022Quote: ... the media was changed to RPMI 1640 without phenol red supplemented with 6 μg/mL propidium iodide (Biotium). Cells were infected at an MOI of 100 for 2 hours ...
-
bioRxiv - Physiology 2021Quote: ... and Di-8-ANEPPS (Biotium, #61012, 2 µM) or Wheat Germ Agglutinin Alexa Fluor™-488 Conjugate (Invitrogen™ ...
-
bioRxiv - Neuroscience 2022Quote: ... Pluronic F127 and Rhod-2/AM from Biotium, buprenorphine from Champion Alstoe Animal Health ...
-
bioRxiv - Physiology 2022Quote: ... Sections were stained with RedDot 2 nuclear stain (Biotium) and WGA Oregon Green (Fisher) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product by mitochondrial dehydrogenase enzymes ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
-
bioRxiv - Cancer Biology 2022Quote: ... using 2 μg/ml of 405-Alexa-NHS ester (Biotium).
-
bioRxiv - Cell Biology 2020Quote: ... cells were treated with 5 μmol/L NucView™ 488 caspase-3 substrate (Biotium). Images were obtained in phase contrast and fluorescence mode using a x10 objective and an IncuCyte FLR time-lapse fluorescence microscope (Essen Bioscience) ...
-
bioRxiv - Microbiology 2022Quote: ... Resulting reactions were run on 2% agarose gel with GelGreen (Biotium) before imaging using an EZ Dock Imager (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2023Quote: The free radical probe 2’,7’-dichlorodihydrofluoresceindiacetate (H2DCFDA) (Biotium, Fremont, CA) was used to quantify ROS ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 2 μl of 1 mg/ml Propidium Iodide (40017, Biotium). The cell solution was incubated for 30 minu at room temperature in the dark ...
-
bioRxiv - Physiology 2023Quote: ... followed by donkey anti-rat CF750 (2 µg/mL, 20857, Biotium). Slides were imaged with the Slideview VS200 (Olympus ...
-
bioRxiv - Genomics 2024Quote: ... DNA was loaded onto 2% agarose gel including 1X GelRed (Biotium).
-
bioRxiv - Neuroscience 2022Quote: ... Sections were washed with PBS (3 × 5 min) and treated with Trueblack (Biotium, Cat# 23007) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were replaced with medium containing 5 µM NucView 488 caspase 3 substrate (Biotium Inc., #10403). After 30 min incubation at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... 10µL of reaction was loaded onto 2% agarose gel with GelGreen (Biotium) and ran for 30 minutes at 100V and visualized using an EZ Dock Imager (Bio-Rad) ...
-
bioRxiv - Cell Biology 2023Quote: ... F(ab’)2-goat anti-mouse IgG CF568 (Biotium Cat# 20109, RRID:AB_10557119), F(ab’)2-goat anti-rabbit IgG CF568 (Biotium Cat# 20099 ...
-
bioRxiv - Cell Biology 2023Quote: ... F(ab’)2-goat anti-rabbit IgG CF568 (Biotium Cat# 20099, RRID:AB_10563029), F(ab’)2-goat anti-mouse IgG Alexa 555 (Thermo Fisher Scientific Cat# A-21425 ...