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Citations for VWR :
1 - 50 of 132 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... RT-PCR was performed with RedTaq Polymerase (VWR) with primers 51-Scorpine-F and 117-CP-ctrl-R on Sco-CP (1267bp) ...
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bioRxiv - Immunology 2024Quote: ... RT-PCR was performed with RedTaq Polymerase (VWR) using primers 154-CP-probe-F & 155-CP-probe-R ...
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bioRxiv - Genomics 2023Quote: SDS treated cells were resuspended in 400 μl of RT mix (final concentration of 1x RT buffer, 500 mM dNTP, 10 mM Biotinylated RT primers, 7.5% PEG 6000 (VWR, 101443-484), 0.4U/ml SUPERase•In™ RNase Inhibitor ...
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bioRxiv - Immunology 2024Quote: ... and RT-PCR was performed with RedTaq Polymerase (VWR) using primers 340-CP-Taq-F and 264-qRel-R2 ...
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bioRxiv - Systems Biology 2021Quote: ... Primers were arrayed in skirted 96 well PCR plates (VWR) using an acoustic liquid handling robot (Labcyte Echo 550 ...
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bioRxiv - Systems Biology 2020Quote: ... Primers were arrayed in skirted 96 well PCR plates (VWR) using an acoustic liquid handling robot (Labcyte Echo 550 ...
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bioRxiv - Systems Biology 2023Quote: ... Primers were arrayed in skirted 96-well PCR plates (VWR) using an acoustic liquid handling robot (Labcyte Echo 550 ...
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bioRxiv - Pathology 2021Quote: Viral RNA was quantitated using single-step RT-quantitative real-time PCR (Quanta qScript One-Step RT-qPCR Kit, QuantaBio, Beverly, MA; VWR; #76047-082) with primers and TaqMan® probes targeting the SARS-CoV-2 E gene as previously described (62) ...
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bioRxiv - Cell Biology 2021Quote: ... set inside an oven (VWR) at 60°C for 2 hours to initiate the crosslinking ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed in 2X saline-sodium citrate (SSC) buffer containing 10% deionized formamide (VWR) and hybridized with fluorescently labeled oligonucleotide probe mixture for 4-16 hours at 37°C in hybridization buffer (2X SSC ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-PCR was performed on previously generated cDNA (8 ng) using KOD Hot Start DNA Polymerase (VWR CA80511-386). The primer sequences for the target transcripts and number of cycles are outlined in Table 3 ...
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bioRxiv - Neuroscience 2021Quote: ... The SSC was then removed from the sections and the coverslips were mounted onto SuperFrost plus (VWR) glass slides using 10 μL SuperFrost gold antifade mountant (Life Technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... antibiotic-resistant colonies were re-streaked and verified by colony PCR using specific primers and Red Taq DNA polymerase master mix (VWR International).
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bioRxiv - Physiology 2020Quote: ... RNA probes were stored in 25% formamide/2x saline-sodium citrate (25% FA/2x SSC; VWR Chemicals, Leicestershire, UK) at −20°C for long-term storage.
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bioRxiv - Cancer Biology 2020Quote: ... the exons of interest in PD-L1 gene were amplified by using specific primers (Table 5) with a PCR thermal cycler (VWR Thermocycler, Leicestershire, England). PCR procedures were performed with a standard reaction mixture (4 μl/sample of Phusion GC Buffer (Thermo Fischer Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... 10% formamide and 2x saline sodium citrate (SSC) supplemented fresh each experiment with 0.1% Tween-20 and 2 μg/mL nuclease-free BSA (VWR, 0332-25G)) ...
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bioRxiv - Developmental Biology 2019Quote: ... 3 minutes), once with 2X SSC (room temperature, 3 minutes) and then treated with 50 ug/mL of RNase A (VWR 1247C393) in 2X SSC for 45 minutes at 37°C by overlaying the sample with 500ul of RNAse solution and incubating in a humidified chamber ...
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bioRxiv - Developmental Biology 2023Quote: ... the same set of Axygen® FXF-384-XL-R-S tips (VWR) were used for the entire separation protocol and were rinsed with 5ul of G&T wash buffer at the end of the separation step ...
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bioRxiv - Neuroscience 2020Quote: ... Each syringe and tubing set contained a 30 mL luer lock glass syringe (VWR) connected to Tygon FEP-lined tubing (Cole-Parmer) ...
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bioRxiv - Neuroscience 2021Quote: ... set at 30 μm and mounted on Superfrost Plus Micro slides (VWR, 48311-703). Next ...
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bioRxiv - Neuroscience 2019Quote: ... A total of 5 µL of Ant-miR-218 or Ant-Scrambled were administered per nostril with a 10XL pipette tip (VWR, Ville Mont-Royal, Quebec, Canada) attached to a P10 micropipette (Corning ...
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bioRxiv - Systems Biology 2020Quote: ... and reverse transcription (RT)-qPCR assays were done using qScript (VWR) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and reverse transcription (RT)-qPCR assays were done using qScript (VWR) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: All steps prior to RT were performed on charged glass slides (VWR). This eliminates the uneasy task of placing a tissue section into the Visium capture area ...
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bioRxiv - Neuroscience 2023Quote: ... blocked for 1hr at RT with 5% normal donkey serum (NDS, VWR) in PBS with 0.25% Triton-X (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... mechanically homogenized and afterward fixed with 2% Paraformaldehyde (10 min, RT; VWR). To minimize clumps ...
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bioRxiv - Synthetic Biology 2023Quote: ... the PCR plate was sealed with PCR film (VWR 60941-078) and the following reaction was carried out on a thermocycler ...
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bioRxiv - Cancer Biology 2020Quote: ... Sections were dried at RT for 30 minutes on a glass coverslip (VWR), and either stored dried at −80°C or directly processed by rehydration in 1XPBS followed by fixation in 4% PFA for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed (4% formaldehyde, VWR International, in PBS; 10 min. at RT), washed (2 × 5 min ...
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bioRxiv - Microbiology 2020Quote: ... solution to a 96 well PCR plate (FLAT 96 WELL PCR PLATE, VWR) and 5 µl of enzyme solution ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCRs were carried out using the KAPA3G Plant PCR Kit (Kapa Biosystems, VWR) using the following cycling conditions for the phylogenetically informative loci ...
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bioRxiv - Cell Biology 2023Quote: ... strips were covered with Axygen PCR-tube caps (VWR, PCR-02-FCP-C), centrifuged and placed on dry ice until storage at −80°C for further use ...
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bioRxiv - Neuroscience 2020Quote: ... three times at RT and then incubated with Thiazine-Red (0.05%, cat#27419.123, VWR) for 20 min ...
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bioRxiv - Bioengineering 2022Quote: ... was incubated for 30 minutes at RT and Vector VIP Peroxidase Substrate Kit (VWR) was used to develop staining ...
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bioRxiv - Molecular Biology 2023Quote: ... After PCR amplification using NEBNext High-Fidelity 2 × PCR Master Mix (VWR 102500-096) and post-PCR cleanup using SPRI beads (Beckman Coulter A63880) ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in ~100 μL of culture supernatant and 3 μL deposited on a pre-set 65 μL drop of SC+ 2% glucose+ 2% agar gel medium on a circular glass coverslip (#1, VWR) that was encircled by an adhesive silicon ring ...
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bioRxiv - Genomics 2022Quote: ... The spore suspension was set to 2·108 spores mL-1 using a Bürker-Türk haemocytometer (VWR, Amsterdam, The Netherlands) and kept on ice until further use.
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bioRxiv - Genomics 2019Quote: ... The mix was filtered through a set of sieves (1 mm/500 μm/38 μm × 200 mm diameter, (VWR, Sweden). The content of the last sieve was transferred into a falcon tube containing 20 ml of 60% sucrose solution ...
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bioRxiv - Biochemistry 2020Quote: ... Two sets of spectra ranging from 230 to 750 nm were recorded simultaneously using two UV-3100PC UV/Vis spectrophotometers (VWR, Radnor, PA). In the first device ...
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bioRxiv - Neuroscience 2021Quote: ... Cryosections were obtained using a Leica cryostat set at 20-30 μm and mounted on Superfrost Plus Micro slides (VWR, 48311-703). On the day of staining ...
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bioRxiv - Developmental Biology 2024Quote: ... at 14 μm and collected sections into 4 sets of alternate series of SuperFrost Plus microscopy slides (VWR International, Randor, PA, USA). Slides were allowed to dry completely and stored at −80°C until processing ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS at pH 10 at room temperature on an orbital shaker set at 150 rpm (no. 57018-754VWR; VWR International, Brisbane, CA). After 48 h ...
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bioRxiv - Microbiology 2023Quote: ... for 30 minutes at RT before being mounted on a microscope slide using VECTASHIELD (VWR, #H-1000) and sealed in nail polish ...
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bioRxiv - Immunology 2023Quote: ... into empty 96-well PCR plates (VWR). FACSDiva software (Becton Dickinson ...
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bioRxiv - Developmental Biology 2022Quote: ... Later blocking was done for 1hour at RT on a rocker with a blocking buffer consisting of 0.1% Triton X-100 and 5% goat serum (VWR). The organoids were incubated overnight in the primary antibodies (File S6 ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated for 1-2 hours at RT with primary antibodies diluted in Odyssey Blocking Buffer + 0.2% Tween (VWR). Next ...
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bioRxiv - Microbiology 2020Quote: ... or 0.4 µM (cesA) of each primer (Table 3) and 60 ng of DNA (measured by a MySpec spectrophotometer, VWR International). qPCR reaction conditions for the panC detection followed the master mix supplier’s protocol ...
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bioRxiv - Zoology 2020Quote: ... PCR reactions were performed in a 55 μl volumes containing: 25 μl Hot Start PCR-to-Gel Master Mix (VWR brand); 2 μl of primer mix (25 pmol each of forward and reverse primer) ...
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bioRxiv - Biochemistry 2019Quote: ... E1A alternatively spliced isoforms were amplified from 3 μl cDNA by PCR in 50 μl reactions with the KAPA HiFi PCR Kit (Peqlab/VWR, Darmstadt, Germany) using primers E1A_ Exon1_forward 5’-GTT TTC TCC TCC GAG CCG CTC CGA-3’ and E1A_Exon2_reverse 5’-CTC AGG CTC AGG TTC AGA CAC AGG-3’ at final concentrations of 500 nM ...
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bioRxiv - Molecular Biology 2023Quote: ... an adapter PCR reaction was performed as follows: All 8 tubes of a 50 µL PCR strip tube (VWR, catalog # 490003-606) were filled with 2500 ng DNA template ...