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Citations for VWR :
1 - 50 of 289 citations for ssc mir 136 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: Viral RNA was quantitated using single-step RT-quantitative real-time PCR (Quanta qScript One-Step RT-qPCR Kit, QuantaBio, Beverly, MA; VWR; #76047-082) with primers and TaqMan® probes targeting the SARS-CoV-2 E gene as previously described (62) ...
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bioRxiv - Genomics 2023Quote: ... dissolved in DMSO (VWR 97063-136) and was put back for nascent RNA metabolic labeling ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative real-time PCR was performed using the PerfecCTa SYBR Green FastMix (VWR, catalog # 101414-280) on the Step-One Plus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... Virus titers were quantified and titrated by real-time PCR (QuantaBio ToughMix®, VWR International, Radner, PA) with a TCID50/ml equivalent as previously described (34) ...
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bioRxiv - Cancer Biology 2023Quote: ... Expression of genes of interest was quantified by real-time PCR using SYBR Green method (VWR QUNT95072). Primers used are detailed in supplementary methods ...
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bioRxiv - Cancer Biology 2022Quote: ... which was first solubilized in dimethyl sulfoxide (97063-136, VWR) to a concentration of 50 mg/mL ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots were prepared in ultra-pure grade DMSO (VWR; 97063-136) and stored at −20°C until use ...
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bioRxiv - Cancer Biology 2024Quote: ... and Ruxolitinib (Selleckchem # S1378) were solubilized in DMSO (VWR #97063-136) prior to treatment.
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bioRxiv - Physiology 2022Quote: ... samples were placed in cassettes (cat#18000-136; VWR, Radnor, Pennsylvania, USA) and treated with 90% ethanol for 30 min on a stir plate and then treated with 3 passages of 100% ethanol for 45 min each ...
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bioRxiv - Synthetic Biology 2020Quote: ... RT-PCR was performed with RedTaq Polymerase (VWR) with primers 51-Scorpine-F and 117-CP-ctrl-R on Sco-CP (1267bp) ...
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bioRxiv - Immunology 2024Quote: ... RT-PCR was performed with RedTaq Polymerase (VWR) using primers 154-CP-probe-F & 155-CP-probe-R ...
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bioRxiv - Immunology 2024Quote: ... and RT-PCR was performed with RedTaq Polymerase (VWR) using primers 340-CP-Taq-F and 264-qRel-R2 ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were tested for mycoplasma every 3 months using the Genlantis MycoScope PCR Detection Kit (VWR, 10497-508) and were confirmed to be free of mycoplasma contamination ...
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bioRxiv - Neuroscience 2020Quote: ... three times at RT and then incubated with Thiazine-Red (0.05%, cat#27419.123, VWR) for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... pHrodo™ iFL Red STP Ester was diluted to 2 mM in DMSO (VWR Life Science, 97063-136). Antibody was brought up in DPBS (2 mg/mL final concentration for Dll4 polyclonal antibody [Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked with TBS-T (20 mM Tris-HCl, pH 7.6, 136 mM NaCl, 0.1% Tween-20) containing 3% BSA (VWR Life Science, VWRV0332) for 1h ...
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bioRxiv - Immunology 2021Quote: ... A single incision was made above the quadriceps and a 2 mm or 3 mm biopsy punch (VWR, 21909-132, −136) was used to make a fullthickness muscle defect ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed in 2X saline-sodium citrate (SSC) buffer containing 10% deionized formamide (VWR) and hybridized with fluorescently labeled oligonucleotide probe mixture for 4-16 hours at 37°C in hybridization buffer (2X SSC ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCRs were carried out using the KAPA3G Plant PCR Kit (Kapa Biosystems, VWR) using the following cycling conditions for the phylogenetically informative loci ...
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bioRxiv - Bioengineering 2022Quote: ... was incubated for 30 minutes at RT and Vector VIP Peroxidase Substrate Kit (VWR) was used to develop staining ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-PCR was performed on previously generated cDNA (8 ng) using KOD Hot Start DNA Polymerase (VWR CA80511-386). The primer sequences for the target transcripts and number of cycles are outlined in Table 3 ...
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bioRxiv - Neuroscience 2021Quote: ... The SSC was then removed from the sections and the coverslips were mounted onto SuperFrost plus (VWR) glass slides using 10 μL SuperFrost gold antifade mountant (Life Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lines were monitored for Mycoplasma contamination regularly using Mycoplasma Detection Kit (VWR, 89510-164).
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bioRxiv - Cell Biology 2020Quote: ... ECL detection system (VWR) and SuperSignal West Dura (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed with the kit TaqPolimerase 2X (VWR) using the following PCR program ...
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bioRxiv - Physiology 2020Quote: ... RNA probes were stored in 25% formamide/2x saline-sodium citrate (25% FA/2x SSC; VWR Chemicals, Leicestershire, UK) at −20°C for long-term storage.
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bioRxiv - Developmental Biology 2020Quote: ... 10% formamide and 2x saline sodium citrate (SSC) supplemented fresh each experiment with 0.1% Tween-20 and 2 μg/mL nuclease-free BSA (VWR, 0332-25G)) ...
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bioRxiv - Developmental Biology 2019Quote: ... 3 minutes), once with 2X SSC (room temperature, 3 minutes) and then treated with 50 ug/mL of RNase A (VWR 1247C393) in 2X SSC for 45 minutes at 37°C by overlaying the sample with 500ul of RNAse solution and incubating in a humidified chamber ...
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bioRxiv - Biochemistry 2019Quote: ... E1A alternatively spliced isoforms were amplified from 3 μl cDNA by PCR in 50 μl reactions with the KAPA HiFi PCR Kit (Peqlab/VWR, Darmstadt, Germany) using primers E1A_ Exon1_forward 5’-GTT TTC TCC TCC GAG CCG CTC CGA-3’ and E1A_Exon2_reverse 5’-CTC AGG CTC AGG TTC AGA CAC AGG-3’ at final concentrations of 500 nM ...
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bioRxiv - Biochemistry 2023Quote: ... using ECL Western Blotting Detection Reagent (VWR, RPN2106).
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bioRxiv - Genomics 2023Quote: SDS treated cells were resuspended in 400 μl of RT mix (final concentration of 1x RT buffer, 500 mM dNTP, 10 mM Biotinylated RT primers, 7.5% PEG 6000 (VWR, 101443-484), 0.4U/ml SUPERase•In™ RNase Inhibitor ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each fin bud was first homogenized in 50 μL of Buffer RLT (supplemented with β-mercaptoethanol per kit recommendation) for 1 min at RT in a 1.5 mL centrifuge tube using a cordless motor (VWR Catalog # 47747-370) and pestle (USA Scientific Catalog # 1415-5390) ...
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bioRxiv - Biophysics 2022Quote: ... ECL Western blotting detection reagent (GE) or ECL prime (VWR) were used for detection.
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bioRxiv - Microbiology 2022Quote: ... and developed with Amersham ECL Select chemiluminescent detection reagent (VWR) according to the manufacturers’ protocols ...
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bioRxiv - Neuroscience 2019Quote: ... A total of 5 µL of Ant-miR-218 or Ant-Scrambled were administered per nostril with a 10XL pipette tip (VWR, Ville Mont-Royal, Quebec, Canada) attached to a P10 micropipette (Corning ...
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bioRxiv - Cell Biology 2019Quote: ... Detection was carried out by using AceGlow (VWR Peqlab, Erlangen, Germany).
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bioRxiv - Cell Biology 2024Quote: ... HRP was detected using ECL western blotting detection reagents (VWR, # RPN2106). Chemiluminescence was detected using and X-ray films (Bema ...
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bioRxiv - Systems Biology 2020Quote: ... and reverse transcription (RT)-qPCR assays were done using qScript (VWR) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and reverse transcription (RT)-qPCR assays were done using qScript (VWR) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: All steps prior to RT were performed on charged glass slides (VWR). This eliminates the uneasy task of placing a tissue section into the Visium capture area ...
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bioRxiv - Neuroscience 2023Quote: ... blocked for 1hr at RT with 5% normal donkey serum (NDS, VWR) in PBS with 0.25% Triton-X (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... mechanically homogenized and afterward fixed with 2% Paraformaldehyde (10 min, RT; VWR). To minimize clumps ...
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bioRxiv - Molecular Biology 2022Quote: ... Homogenates were sonicated three times for 30s each time at room temperature in a water bath sonicator (VWR, 97043-960). Samples were rested on ice for 30s between each cycle ...
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bioRxiv - Synthetic Biology 2023Quote: ... the PCR plate was sealed with PCR film (VWR 60941-078) and the following reaction was carried out on a thermocycler ...
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bioRxiv - Immunology 2022Quote: ... 1 time with 1ml of 0.1M Na2CO3 (VWR), 1 time with 1ml of freshly prepared 2M Urea (VWR ...
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bioRxiv - Molecular Biology 2023Quote: ... and 4 times with isopropanol (VWR,9084-03) and 2 times with distilled water for 3 minutes (min ...
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bioRxiv - Cell Biology 2019Quote: ... Activity was detected using the AceGlow blotting detection system (VWR Peqlab, Erlangen, Germany).
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bioRxiv - Cancer Biology 2020Quote: ... Sections were dried at RT for 30 minutes on a glass coverslip (VWR), and either stored dried at −80°C or directly processed by rehydration in 1XPBS followed by fixation in 4% PFA for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed (4% formaldehyde, VWR International, in PBS; 10 min. at RT), washed (2 × 5 min ...
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bioRxiv - Plant Biology 2019Quote: ... Colonies were evaluated by colony PCR followed by plasmid isolation from overnight culture using peqGOLD Plasmid Miniprep Kit I (VWR). All sequences were confirmed by Sanger sequencing (Sequencing Core Facility ...