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Citations for VWR :
1 - 50 of 141 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... RT-PCR was performed with RedTaq Polymerase (VWR) with primers 51-Scorpine-F and 117-CP-ctrl-R on Sco-CP (1267bp) ...
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bioRxiv - Immunology 2024Quote: ... RT-PCR was performed with RedTaq Polymerase (VWR) using primers 154-CP-probe-F & 155-CP-probe-R ...
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bioRxiv - Genomics 2023Quote: SDS treated cells were resuspended in 400 μl of RT mix (final concentration of 1x RT buffer, 500 mM dNTP, 10 mM Biotinylated RT primers, 7.5% PEG 6000 (VWR, 101443-484), 0.4U/ml SUPERase•In™ RNase Inhibitor ...
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bioRxiv - Immunology 2024Quote: ... and RT-PCR was performed with RedTaq Polymerase (VWR) using primers 340-CP-Taq-F and 264-qRel-R2 ...
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bioRxiv - Systems Biology 2021Quote: ... Primers were arrayed in skirted 96 well PCR plates (VWR) using an acoustic liquid handling robot (Labcyte Echo 550 ...
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bioRxiv - Systems Biology 2020Quote: ... Primers were arrayed in skirted 96 well PCR plates (VWR) using an acoustic liquid handling robot (Labcyte Echo 550 ...
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bioRxiv - Systems Biology 2023Quote: ... Primers were arrayed in skirted 96-well PCR plates (VWR) using an acoustic liquid handling robot (Labcyte Echo 550 ...
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bioRxiv - Pathology 2021Quote: Viral RNA was quantitated using single-step RT-quantitative real-time PCR (Quanta qScript One-Step RT-qPCR Kit, QuantaBio, Beverly, MA; VWR; #76047-082) with primers and TaqMan® probes targeting the SARS-CoV-2 E gene as previously described (62) ...
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bioRxiv - Genomics 2023Quote: ... 25% glycerol (VWR, cat. no. RC3290-32), 0.1 mM EDTA (Fisher ...
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bioRxiv - Genomics 2023Quote: ... and 10% (v/v) glycerol (VWR, Cat. RC3290-32) diluted in nuclease-free water) ...
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bioRxiv - Cell Biology 2021Quote: ... set inside an oven (VWR) at 60°C for 2 hours to initiate the crosslinking ...
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bioRxiv - Microbiology 2021Quote: ... 32 or 64 μg/ml of cefotaxime (VWR International Ltd). Cell proliferation was determined by measuring the optical density at 600 nm for 8 or 12 h every 15 min ...
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bioRxiv - Plant Biology 2020Quote: ... and cover glass (VWR® cover glass: 22 x 32 mm). Observations were conducted with a ZEISS LSM 800 microscope using the 10x objective ...
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bioRxiv - Biochemistry 2023Quote: ... Hydrochloric acid (HCl; 32% w/v AnalaR) was supplied by VWR International (Radnor ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-PCR was performed on previously generated cDNA (8 ng) using KOD Hot Start DNA Polymerase (VWR CA80511-386). The primer sequences for the target transcripts and number of cycles are outlined in Table 3 ...
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bioRxiv - Bioengineering 2021Quote: ... antibiotic-resistant colonies were re-streaked and verified by colony PCR using specific primers and Red Taq DNA polymerase master mix (VWR International).
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bioRxiv - Neuroscience 2020Quote: ... Trace element grade hydrogen peroxide (32%, BDH Aristar® Ultra grade, VWR Analytical) was added to the sample in equal volume to the amount of nitric acid added and then incubated at 70°C for 20 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... the exons of interest in PD-L1 gene were amplified by using specific primers (Table 5) with a PCR thermal cycler (VWR Thermocycler, Leicestershire, England). PCR procedures were performed with a standard reaction mixture (4 μl/sample of Phusion GC Buffer (Thermo Fischer Scientific) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and transferred pupae from each seed paper to separate 32 oz HDPE plastic cages (VWR). Eclosing males and females were able to mate with each other in the cages and had access to 10% sucrose solution ...
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bioRxiv - Immunology 2023Quote: ... as described [32] The solution was then filtered through a 100 µm nylon cell strainer (VWR) and centrifuged at 400 x g for 10 minutes at 4°C and the supernatant was discarded ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Western blotting was performed essentially as described before.32 Using Gentle ReView™ Stripping Buffer (VWR, #19G0856497), several protein detections were done on the same membrane to conserve time and samples ...
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bioRxiv - Developmental Biology 2023Quote: ... the same set of Axygen® FXF-384-XL-R-S tips (VWR) were used for the entire separation protocol and were rinsed with 5ul of G&T wash buffer at the end of the separation step ...
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bioRxiv - Synthetic Biology 2019Quote: ... The PDMS microchannels were then sealed by plasma bonding them onto glass cover slips (24 × 32 mm, thickness 1.5, VWR) using oxygen plasma (15 s at 0.3mbar ...
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bioRxiv - Neuroscience 2020Quote: ... Each syringe and tubing set contained a 30 mL luer lock glass syringe (VWR) connected to Tygon FEP-lined tubing (Cole-Parmer) ...
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bioRxiv - Neuroscience 2021Quote: ... set at 30 μm and mounted on Superfrost Plus Micro slides (VWR, 48311-703). Next ...
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bioRxiv - Cell Biology 2023Quote: ... The thin polyacrylamide-based substrate was polymerized between two different glass coverslips (#631-0162, diameter 32 millimeters, thickness No. 1, VWR) prepared as follows ...
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bioRxiv - Microbiology 2023Quote: ... Then 20 μL of hemolymph per each sample were digested with 0.5 mL of 32% ultrapure hydrochloric acid (VWR Chemicals) under heating conditions (60 °C ...
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bioRxiv - Neuroscience 2019Quote: ... A total of 5 µL of Ant-miR-218 or Ant-Scrambled were administered per nostril with a 10XL pipette tip (VWR, Ville Mont-Royal, Quebec, Canada) attached to a P10 micropipette (Corning ...
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bioRxiv - Neuroscience 2023Quote: ... and 10 – 20 mosquitoes were sorted into a behavioral container made from a 32-ounce HDPE plastic cup (VWR #89009-668). The behavioral container was prepared by cutting a 10 cm hole in the lid with a razor blade and covering the cup with a 20 cm x 20 cm piece of white 0.8 mm polyester mosquito netting (American Home & Habit Inc ...
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bioRxiv - Systems Biology 2020Quote: ... and reverse transcription (RT)-qPCR assays were done using qScript (VWR) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and reverse transcription (RT)-qPCR assays were done using qScript (VWR) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: A perfusion chamber was prepared by sandwiching a cover glass (BDH, 22 × 32 mm #1) on top of acid and plasma cleaned cover glass (VWR 24 × 50mm #1.5) containing two lines of Corning High Vacuum Grease ...
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bioRxiv - Genomics 2022Quote: All steps prior to RT were performed on charged glass slides (VWR). This eliminates the uneasy task of placing a tissue section into the Visium capture area ...
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bioRxiv - Neuroscience 2023Quote: ... blocked for 1hr at RT with 5% normal donkey serum (NDS, VWR) in PBS with 0.25% Triton-X (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... mechanically homogenized and afterward fixed with 2% Paraformaldehyde (10 min, RT; VWR). To minimize clumps ...
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bioRxiv - Synthetic Biology 2023Quote: ... the PCR plate was sealed with PCR film (VWR 60941-078) and the following reaction was carried out on a thermocycler ...
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bioRxiv - Cancer Biology 2020Quote: ... Sections were dried at RT for 30 minutes on a glass coverslip (VWR), and either stored dried at −80°C or directly processed by rehydration in 1XPBS followed by fixation in 4% PFA for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed (4% formaldehyde, VWR International, in PBS; 10 min. at RT), washed (2 × 5 min ...
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bioRxiv - Microbiology 2020Quote: ... solution to a 96 well PCR plate (FLAT 96 WELL PCR PLATE, VWR) and 5 µl of enzyme solution ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCRs were carried out using the KAPA3G Plant PCR Kit (Kapa Biosystems, VWR) using the following cycling conditions for the phylogenetically informative loci ...
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bioRxiv - Cell Biology 2023Quote: ... strips were covered with Axygen PCR-tube caps (VWR, PCR-02-FCP-C), centrifuged and placed on dry ice until storage at −80°C for further use ...
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bioRxiv - Neuroscience 2020Quote: ... three times at RT and then incubated with Thiazine-Red (0.05%, cat#27419.123, VWR) for 20 min ...
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bioRxiv - Bioengineering 2022Quote: ... was incubated for 30 minutes at RT and Vector VIP Peroxidase Substrate Kit (VWR) was used to develop staining ...
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bioRxiv - Molecular Biology 2023Quote: ... After PCR amplification using NEBNext High-Fidelity 2 × PCR Master Mix (VWR 102500-096) and post-PCR cleanup using SPRI beads (Beckman Coulter A63880) ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in ~100 μL of culture supernatant and 3 μL deposited on a pre-set 65 μL drop of SC+ 2% glucose+ 2% agar gel medium on a circular glass coverslip (#1, VWR) that was encircled by an adhesive silicon ring ...
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bioRxiv - Genomics 2022Quote: ... The spore suspension was set to 2·108 spores mL-1 using a Bürker-Türk haemocytometer (VWR, Amsterdam, The Netherlands) and kept on ice until further use.
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bioRxiv - Genomics 2019Quote: ... The mix was filtered through a set of sieves (1 mm/500 μm/38 μm × 200 mm diameter, (VWR, Sweden). The content of the last sieve was transferred into a falcon tube containing 20 ml of 60% sucrose solution ...
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bioRxiv - Biochemistry 2020Quote: ... Two sets of spectra ranging from 230 to 750 nm were recorded simultaneously using two UV-3100PC UV/Vis spectrophotometers (VWR, Radnor, PA). In the first device ...
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bioRxiv - Neuroscience 2021Quote: ... Cryosections were obtained using a Leica cryostat set at 20-30 μm and mounted on Superfrost Plus Micro slides (VWR, 48311-703). On the day of staining ...
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bioRxiv - Developmental Biology 2024Quote: ... at 14 μm and collected sections into 4 sets of alternate series of SuperFrost Plus microscopy slides (VWR International, Randor, PA, USA). Slides were allowed to dry completely and stored at −80°C until processing ...