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Citations for VWR :
1 - 50 of 294 citations for hsa mir 32 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: Viral RNA was quantitated using single-step RT-quantitative real-time PCR (Quanta qScript One-Step RT-qPCR Kit, QuantaBio, Beverly, MA; VWR; #76047-082) with primers and TaqMan® probes targeting the SARS-CoV-2 E gene as previously described (62) ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative real-time PCR was performed using the PerfecCTa SYBR Green FastMix (VWR, catalog # 101414-280) on the Step-One Plus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... Virus titers were quantified and titrated by real-time PCR (QuantaBio ToughMix®, VWR International, Radner, PA) with a TCID50/ml equivalent as previously described (34) ...
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bioRxiv - Cancer Biology 2023Quote: ... Expression of genes of interest was quantified by real-time PCR using SYBR Green method (VWR QUNT95072). Primers used are detailed in supplementary methods ...
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bioRxiv - Synthetic Biology 2020Quote: ... RT-PCR was performed with RedTaq Polymerase (VWR) with primers 51-Scorpine-F and 117-CP-ctrl-R on Sco-CP (1267bp) ...
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bioRxiv - Immunology 2024Quote: ... RT-PCR was performed with RedTaq Polymerase (VWR) using primers 154-CP-probe-F & 155-CP-probe-R ...
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bioRxiv - Immunology 2024Quote: ... and RT-PCR was performed with RedTaq Polymerase (VWR) using primers 340-CP-Taq-F and 264-qRel-R2 ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were tested for mycoplasma every 3 months using the Genlantis MycoScope PCR Detection Kit (VWR, 10497-508) and were confirmed to be free of mycoplasma contamination ...
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bioRxiv - Neuroscience 2020Quote: ... three times at RT and then incubated with Thiazine-Red (0.05%, cat#27419.123, VWR) for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... Element calibration curves were generated using ARISTAR ICP Standard Mix (VWR). Samples were introduced by a peristaltic pump with 0.5 mm internal diameter tubing through a MicroMist borosilicate glass nebulizer (Agilent) ...
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bioRxiv - Genomics 2023Quote: ... 25% glycerol (VWR, cat. no. RC3290-32), 0.1 mM EDTA (Fisher ...
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bioRxiv - Microbiology 2020Quote: ... Element calibration curves were generated using ARISTAR ICP Standard Mix (VWR, Radnor, PA). Samples were introduced by peristaltic pump with 0.5 mm internal diameter tubing through a MicroMist borosilicate glass nebulizer (Agilent) ...
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bioRxiv - Immunology 2021Quote: ... Element calibration curves were generated using ARISTAR ICP Standard Mix (VWR, Radnor, PA). Samples were introduced by peristaltic pump with 0.5 mm internal diameter tubing through a MicroMist borosilicate glass nebulizer (Agilent) ...
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bioRxiv - Genomics 2023Quote: ... and 10% (v/v) glycerol (VWR, Cat. RC3290-32) diluted in nuclease-free water) ...
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bioRxiv - Microbiology 2021Quote: ... 32 or 64 μg/ml of cefotaxime (VWR International Ltd). Cell proliferation was determined by measuring the optical density at 600 nm for 8 or 12 h every 15 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCRs were carried out using the KAPA3G Plant PCR Kit (Kapa Biosystems, VWR) using the following cycling conditions for the phylogenetically informative loci ...
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bioRxiv - Plant Biology 2020Quote: ... and cover glass (VWR® cover glass: 22 x 32 mm). Observations were conducted with a ZEISS LSM 800 microscope using the 10x objective ...
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bioRxiv - Biochemistry 2023Quote: ... Hydrochloric acid (HCl; 32% w/v AnalaR) was supplied by VWR International (Radnor ...
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bioRxiv - Bioengineering 2022Quote: ... was incubated for 30 minutes at RT and Vector VIP Peroxidase Substrate Kit (VWR) was used to develop staining ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-PCR was performed on previously generated cDNA (8 ng) using KOD Hot Start DNA Polymerase (VWR CA80511-386). The primer sequences for the target transcripts and number of cycles are outlined in Table 3 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lines were monitored for Mycoplasma contamination regularly using Mycoplasma Detection Kit (VWR, 89510-164).
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bioRxiv - Cell Biology 2020Quote: ... ECL detection system (VWR) and SuperSignal West Dura (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed with the kit TaqPolimerase 2X (VWR) using the following PCR program ...
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bioRxiv - Neuroscience 2020Quote: ... Trace element grade hydrogen peroxide (32%, BDH Aristar® Ultra grade, VWR Analytical) was added to the sample in equal volume to the amount of nitric acid added and then incubated at 70°C for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: Calibration of the device was carried out by spraying deionized water on 9 cm diameter filter paper (Qualitative 415, VWR). In order to understand the relationship between pressure ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and transferred pupae from each seed paper to separate 32 oz HDPE plastic cages (VWR). Eclosing males and females were able to mate with each other in the cages and had access to 10% sucrose solution ...
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bioRxiv - Immunology 2023Quote: ... as described [32] The solution was then filtered through a 100 µm nylon cell strainer (VWR) and centrifuged at 400 x g for 10 minutes at 4°C and the supernatant was discarded ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Western blotting was performed essentially as described before.32 Using Gentle ReView™ Stripping Buffer (VWR, #19G0856497), several protein detections were done on the same membrane to conserve time and samples ...
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bioRxiv - Biochemistry 2019Quote: ... E1A alternatively spliced isoforms were amplified from 3 μl cDNA by PCR in 50 μl reactions with the KAPA HiFi PCR Kit (Peqlab/VWR, Darmstadt, Germany) using primers E1A_ Exon1_forward 5’-GTT TTC TCC TCC GAG CCG CTC CGA-3’ and E1A_Exon2_reverse 5’-CTC AGG CTC AGG TTC AGA CAC AGG-3’ at final concentrations of 500 nM ...
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bioRxiv - Biochemistry 2023Quote: ... using ECL Western Blotting Detection Reagent (VWR, RPN2106).
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bioRxiv - Genomics 2023Quote: SDS treated cells were resuspended in 400 μl of RT mix (final concentration of 1x RT buffer, 500 mM dNTP, 10 mM Biotinylated RT primers, 7.5% PEG 6000 (VWR, 101443-484), 0.4U/ml SUPERase•In™ RNase Inhibitor ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each fin bud was first homogenized in 50 μL of Buffer RLT (supplemented with β-mercaptoethanol per kit recommendation) for 1 min at RT in a 1.5 mL centrifuge tube using a cordless motor (VWR Catalog # 47747-370) and pestle (USA Scientific Catalog # 1415-5390) ...
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bioRxiv - Synthetic Biology 2019Quote: ... The PDMS microchannels were then sealed by plasma bonding them onto glass cover slips (24 × 32 mm, thickness 1.5, VWR) using oxygen plasma (15 s at 0.3mbar ...
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bioRxiv - Cell Biology 2023Quote: ... The thin polyacrylamide-based substrate was polymerized between two different glass coverslips (#631-0162, diameter 32 millimeters, thickness No. 1, VWR) prepared as follows ...
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bioRxiv - Microbiology 2023Quote: ... Then 20 μL of hemolymph per each sample were digested with 0.5 mL of 32% ultrapure hydrochloric acid (VWR Chemicals) under heating conditions (60 °C ...
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bioRxiv - Biophysics 2022Quote: ... ECL Western blotting detection reagent (GE) or ECL prime (VWR) were used for detection.
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bioRxiv - Microbiology 2022Quote: ... and developed with Amersham ECL Select chemiluminescent detection reagent (VWR) according to the manufacturers’ protocols ...
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bioRxiv - Neuroscience 2019Quote: ... A total of 5 µL of Ant-miR-218 or Ant-Scrambled were administered per nostril with a 10XL pipette tip (VWR, Ville Mont-Royal, Quebec, Canada) attached to a P10 micropipette (Corning ...
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bioRxiv - Neuroscience 2023Quote: ... and 10 – 20 mosquitoes were sorted into a behavioral container made from a 32-ounce HDPE plastic cup (VWR #89009-668). The behavioral container was prepared by cutting a 10 cm hole in the lid with a razor blade and covering the cup with a 20 cm x 20 cm piece of white 0.8 mm polyester mosquito netting (American Home & Habit Inc ...
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bioRxiv - Cell Biology 2019Quote: ... Detection was carried out by using AceGlow (VWR Peqlab, Erlangen, Germany).
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bioRxiv - Cell Biology 2024Quote: ... HRP was detected using ECL western blotting detection reagents (VWR, # RPN2106). Chemiluminescence was detected using and X-ray films (Bema ...
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bioRxiv - Systems Biology 2020Quote: ... and reverse transcription (RT)-qPCR assays were done using qScript (VWR) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and reverse transcription (RT)-qPCR assays were done using qScript (VWR) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: A perfusion chamber was prepared by sandwiching a cover glass (BDH, 22 × 32 mm #1) on top of acid and plasma cleaned cover glass (VWR 24 × 50mm #1.5) containing two lines of Corning High Vacuum Grease ...
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bioRxiv - Genomics 2022Quote: All steps prior to RT were performed on charged glass slides (VWR). This eliminates the uneasy task of placing a tissue section into the Visium capture area ...
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bioRxiv - Neuroscience 2023Quote: ... blocked for 1hr at RT with 5% normal donkey serum (NDS, VWR) in PBS with 0.25% Triton-X (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... mechanically homogenized and afterward fixed with 2% Paraformaldehyde (10 min, RT; VWR). To minimize clumps ...
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bioRxiv - Molecular Biology 2022Quote: ... Homogenates were sonicated three times for 30s each time at room temperature in a water bath sonicator (VWR, 97043-960). Samples were rested on ice for 30s between each cycle ...
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bioRxiv - Synthetic Biology 2023Quote: ... the PCR plate was sealed with PCR film (VWR 60941-078) and the following reaction was carried out on a thermocycler ...
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bioRxiv - Immunology 2022Quote: ... 1 time with 1ml of 0.1M Na2CO3 (VWR), 1 time with 1ml of freshly prepared 2M Urea (VWR ...