Labshake search
Citations for VWR :
1 - 50 of 859 citations for Zika Virus Vero Cell Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2020Quote: ... Cell lysate concentrations were measured using Bradford reagent (VWR) or BCA assay (ThermoFisher) ...
-
bioRxiv - Neuroscience 2022Quote: ... DRG were further lysate by sonicated (Vibra-Cell™, VWR) 5 times 10 secs.
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Resistance selection using fixed concentrations of COV-siRNA1 or COV-siRNA1+2 was conducted in Vero E6 cells cultured in DMEM supplemented with 10% FBS (VWR) and 1x Penicillin/Streptomycin (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... Cell lysates were briefly sonicated using a Branson Sonifier 450 (VWR; output 4 ...
-
bioRxiv - Neuroscience 2021Quote: ... Cell lysates were transferred to Phase Lock Gel 2ml tubes (2302830; VWR). Then ...
-
bioRxiv - Microbiology 2021Quote: ... Vero cells were seeded at 60% confluence in 24-well plates equipped with 13 mm round glass coverslips (VWR, cat n. 631-0150). Cells were washed once with PBS before incubation with BetaCoV/Australia/VIC01/2020 diluted in PBS at an MOI=5 ...
-
bioRxiv - Immunology 2020Quote: ... Protein cell lysates were collected by lysing and scraping cells with RIPA Lysis buffer (VWR, Mississauga, Ontario) mixed with protease inhibitor cocktail (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2023Quote: ... Vero E6 (ATCC CRL- 1586) was cultured in DMEM supplemented with 5 % FCS (VWR) and 1 % penicillin−streptomycin−glutamine (PSG ...
-
bioRxiv - Microbiology 2022Quote: ... African green monkey kidney cells (Vero) were maintained in DMEM supplemented with 5% heat-inactivated fetal bovine essence (FBE) (VWR Life Sciences 10803-034), 1% Penicillin/Streptomycin (Gibco ...
-
bioRxiv - Cancer Biology 2024Quote: ... Whole cell lysates were obtained via mechanical lysis using a needle (VWR-TERUMO, AN2138R1) and RIPA cell lysis buffer (Sigma/Merck ...
-
bioRxiv - Cell Biology 2023Quote: ... The cell lysates were completely transferred to polycarbonate tubes and centrifuged (Micro Star 30, VWR) at 25,000 × g ...
-
bioRxiv - Microbiology 2021Quote: ... the virus suspension was mixed 1:1 with peqGOLD TriFast (VWR). Following ...
-
bioRxiv - Neuroscience 2020Quote: ... the lysate was transferred to a 100 μm nylon cell strainer (VWR, ref nr 732-2759) to remove tissue debris and the flow-through was centrifuged at 2600 × g at 4 °C for 1 min to pellet nuclei ...
-
bioRxiv - Cancer Biology 2022Quote: ... cell lysates were generated in ice cold RIPA lysis buffer (20mM Tris-HCl (VWR, 97061-258), pH 7.5 ...
-
bioRxiv - Neuroscience 2021Quote: ... A virus or tracer was injected using a calibrated glass micropipette (VWR) at a rate of 0.3–0.5 μl/min ...
-
bioRxiv - Biochemistry 2022Quote: ... Virus-containing supernatants were filtered (0.45 μm pore size cellulose-acetate filter (VWR)) ...
-
bioRxiv - Microbiology 2021Quote: ... 10 μl of the cell lysates were resolved by standard 10% SDS-PAGE gel and electroblotted onto 0.2 mm nitrocellulose membrane (Biotrace, VWR) using a semi-dry transfer unit (Bio-Rad) ...
-
bioRxiv - Neuroscience 2020Quote: ... rabies virus was delivered by pressure injection (Nanoject II Variable Volume Automatic Injector, VWR) in 23 nl increments over a 3 min and 10 sec interval to a final volume of 500 nl ...
-
bioRxiv - Developmental Biology 2020Quote: ... The pooled virus-containing supernatant was filtered through a 0.45 μM PES membrane (VWR). The filtered supernatant was then spun in an ultracentrifuge for 2 hours at 20,000 g at 4 °C ...
-
bioRxiv - Biophysics 2020Quote: ... The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Pierce, VWR) for 2 h at 4 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Pierce, VWR) for 2 h at 4 °C on a rotator ...
-
bioRxiv - Microbiology 2023Quote: ... The virus band was extracted and desalted on a NAP column (VWR, Cytiva, Illustra NAP) by elution in PBS ...
-
bioRxiv - Bioengineering 2023Quote: ... the virus-particle-containing medium was collected and filtered using a 0.45 µm syringe filter (VWR), and then centrifuged at 25,000 × g for 90 min at 4°C ...
-
bioRxiv - Microbiology 2021Quote: Protein lysate was collected with RIPA buffer (VWR Life Science) supplemented with 2X protease inhibitors (Pierce ...
-
bioRxiv - Cell Biology 2022Quote: The lipidomics solubilization buffer used to resuspend whole-cell lysates and GolgiTAG immunoprecipitates was made of LC/MS grade chloroform (VWR. Cat# 22711.260) and Optima LC/MS grade methanol (Fisher ...
-
bioRxiv - Synthetic Biology 2019Quote: ... the supernatant (containing secreted AAV virus) was collected and filtered through a 0.45-μm syringe filter (VWR). AAV virus was aliquoted into sterile Eppendorf tubes (0.5 mL/tube) ...
-
bioRxiv - Cell Biology 2021Quote: ... P1 virus was propagated to passage 2 (P2) by infecting 50 ml of SF9 (VWR, #EM71104-3) culture and harvesting after 5–7 days as described above ...
-
bioRxiv - Microbiology 2022Quote: ... Virus titers were quantified and titrated by real-time PCR (QuantaBio ToughMix®, VWR International, Radner, PA) with a TCID50/ml equivalent as previously described (34) ...
-
bioRxiv - Cell Biology 2023Quote: ... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
-
bioRxiv - Cancer Biology 2023Quote: ... Lysates were pulled down with either anti-HA Magnetic Beads (VWR PI88836) or IgG (Invitrogen MA5-14453 ...
-
bioRxiv - Cell Biology 2020Quote: ... virus-containing supernatant was collected 48 h later and filtered through a 0.45 μm polyethersulfone filter (VWR 28145-505). For protein-coding constructs ...
-
bioRxiv - Microbiology 2023Quote: ... The virus containing band was extracted from the gradient and desalted on a NAP column (VWR, Cytiva, Illustra NAP) by elution in PBS ...
-
bioRxiv - Genomics 2022Quote: ... The supernatant (which contains the majority of virus) was filtered through a 0.2 μM PES filter (VWR, cat. #97066-214). 25 mL of 40% PEG8000 (JT Baker ...
-
bioRxiv - Immunology 2019Quote: ... Cell monolayers were processed for ICC staining at 24 to 72 hpi depending on virus replication kinetics (as previously described [94]) or stained with Giemsa stain (VWR; 35086). Relative plaque titre was calculated as the plaque titre of a virus stock under the indicated condition divided by its titre under a control condition ...
-
bioRxiv - Genomics 2020Quote: ... The viscous lysate was transferred to a 2ml Phase Lock tube (VWR, Radnor, PA) and extracted twice with Tris-HCl-saturated phenol:chloroform:isoamyl alcohol (25:24:1 ...
-
bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were passed over a Acrodisc PF syringe 0.8/0.2 μm filter (VWR) prior to incubation with HisPurTM cobalt resin (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... Lysates were prepared with 6X SDS sample buffer containing 12% (v/v) β-ME (VWR) and separated on Bolt™ or NuPAGE 4-12% Bis-Tris gels (Invitrogen) ...
-
bioRxiv - Cell Biology 2019Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
-
bioRxiv - Evolutionary Biology 2019Quote: 1 ml of each lysate was first treated with 12.5 μl 1M MgCl2 (VWR Life Science), 0.4 μl DNAse 1 (2000 U/ml ...
-
bioRxiv - Neuroscience 2024Quote: ... We collected the lysates from the wells using RIPA buffer (with phosphatase and protease inhibitors from Pierce VWR) and scraping the wells ...
-
bioRxiv - Cancer Biology 2019Quote: ... Lysates were probed with antibodies diluted in 5% non-fat dry milk in Tris buffered saline (VWR #J640-4L) and 0.1% Tween20 (Fisher Scientific #BP337 ...
-
bioRxiv - Physiology 2023Quote: Post-ultracentrifugation supernatant (S2) from brain lysates were thawed on ice alongside 1 mM Thioflavin T (VWR 103802-652). Thioflavin T (ThT ...
-
bioRxiv - Bioengineering 2021Quote: ... Then 8mL of clarified lysate was added and the column was sealed and placed on ice and nutated (VWR 12620-916) for 10 minutes ...
-
bioRxiv - Plant Biology 2021Quote: ... and 1/5th of the lysate was added into 40 µL lysis buffer and sheared in a sonicator (VWR Aquasonic 250D, 35Khz) for 3 min ...
-
bioRxiv - Microbiology 2019Quote: ... Cells were collected using a cell scraper (VWR) and resuspended in PBS containing 10% FBS and 1% sodium azide (Sigma) ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were filtered through a 100µm cell strainer (VWR) and additional ...
-
bioRxiv - Biochemistry 2022Quote: ... Both cells were filtered through 40 μm cell strainer (VWR) prior to seeding ...
-
bioRxiv - Bioengineering 2020Quote: ... Cells were filtered through a 40 µm cell strainer (VWR) to remove cell clumps ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were detached with a cell scraper (VWR #10062-906) and the entire contents collected into a microcentrifuge tube ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were detached with a cell scraper (VWR, #734-2602), collected into a pre-chilled falcon tube in 3 repeated steps ...