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Citations for VWR :
1 - 50 of 132 citations for West Nile Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
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bioRxiv - Microbiology 2020Quote: Samples were incubated with 10 mM CCCP (prepared in 50% DMSO) for 30 min at RT followed by addition of 10 mM Nile Red (VWR) at 37°C and shaken at 200 rpm for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... raffinose and agar were purchased from VWR (West Chester, PA). C ...
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bioRxiv - Microbiology 2021Quote: ... the virus suspension was mixed 1:1 with peqGOLD TriFast (VWR). Following ...
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bioRxiv - Immunology 2021Quote: ... fixed with 0.5% neutral buffered formalin (VWR International, West Chester, PA), washed again with DPBS ...
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bioRxiv - Neuroscience 2021Quote: ... A virus or tracer was injected using a calibrated glass micropipette (VWR) at a rate of 0.3–0.5 μl/min ...
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bioRxiv - Cell Biology 2022Quote: ... Patch pipettes were pulled from borosilicate glass capillaries (VWR, West Chester, PA), with a P-87 micropipette puller (Sutter instruments ...
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bioRxiv - Biochemistry 2022Quote: ... Virus-containing supernatants were filtered (0.45 μm pore size cellulose-acetate filter (VWR)) ...
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bioRxiv - Cell Biology 2020Quote: ... 10% fetal bovine serum (Seradigm 1500-500; VWR International, West Chester, PA, USA), penicillin and streptomycin (15140 ...
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bioRxiv - Physiology 2020Quote: ... They were directly mounted onto slides (Superfrost Plus; VWR International, West Chester, PA) in four series with every fifth section on the same slide ...
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bioRxiv - Neuroscience 2019Quote: ... and fed twice daily with Adult Zebrafish Complete Diet (VWR, West Chester, PA) and once daily with brine shrimp (Artemia) ...
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bioRxiv - Neuroscience 2020Quote: ... rabies virus was delivered by pressure injection (Nanoject II Variable Volume Automatic Injector, VWR) in 23 nl increments over a 3 min and 10 sec interval to a final volume of 500 nl ...
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bioRxiv - Developmental Biology 2020Quote: ... The pooled virus-containing supernatant was filtered through a 0.45 μM PES membrane (VWR). The filtered supernatant was then spun in an ultracentrifuge for 2 hours at 20,000 g at 4 °C ...
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bioRxiv - Pathology 2021Quote: ... blood was collected in ethylenediaminetetraacetic acid (EDTA)-containing vacutainer tubes (VWR, West Chester, Pennsylvania) and PBMCs were isolated by density gradient centrifugation using Ficoll-Paque (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... The virus band was extracted and desalted on a NAP column (VWR, Cytiva, Illustra NAP) by elution in PBS ...
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bioRxiv - Immunology 2022Quote: Joint or gut tissues were fixed with 10% neutral formalin solution (VWR, West Chester, PA), and the fixed samples were prepared and stained with H&E as described (39) ...
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bioRxiv - Neuroscience 2023Quote: ... small pieces of filter paper (2.5 cm in diameter) (VWR, Whatman VWR, West Chester PA) were soaked in 50 μl of solvent (DMSO ...
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bioRxiv - Plant Biology 2023Quote: ... The bacteria were kept in 15% (v/v) glycerol (VWR International, West Chester PA, USA) diluted in water until use ...
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bioRxiv - Neuroscience 2023Quote: ... small pieces of filter paper (2.5 cm in diameter) (VWR, Whatman VWR, West Chester PA) were soaked in 50 μl of solvent (DMSO ...
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bioRxiv - Bioengineering 2023Quote: ... the virus-particle-containing medium was collected and filtered using a 0.45 µm syringe filter (VWR), and then centrifuged at 25,000 × g for 90 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... a small piece of filter paper (2.5 cm in diameter) (VWR, Whatman VWR, West Chester PA) soaked in either solvent (DMSO ...
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bioRxiv - Neuroscience 2023Quote: ... a small piece of filter paper (2.5 cm in diameter) (VWR, Whatman VWR, West Chester PA) soaked in either solvent (DMSO ...
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bioRxiv - Synthetic Biology 2019Quote: ... the supernatant (containing secreted AAV virus) was collected and filtered through a 0.45-μm syringe filter (VWR). AAV virus was aliquoted into sterile Eppendorf tubes (0.5 mL/tube) ...
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bioRxiv - Cell Biology 2021Quote: ... P1 virus was propagated to passage 2 (P2) by infecting 50 ml of SF9 (VWR, #EM71104-3) culture and harvesting after 5–7 days as described above ...
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bioRxiv - Microbiology 2022Quote: ... Virus titers were quantified and titrated by real-time PCR (QuantaBio ToughMix®, VWR International, Radner, PA) with a TCID50/ml equivalent as previously described (34) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The capillary pipettes were made of borosilicate glass tubing (VWR micropipettes; VWR Co., West Chester, PA, USA) with DC resistance of 3-5 mΩ ...
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bioRxiv - Neuroscience 2023Quote: ... 1:12 series of sections were quenched for 5 min using 10% H2O2 (VWR, West Sussex, UK) and methanol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
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bioRxiv - Biophysics 2022Quote: ... were purchased from SPI Supplies (West Chester, PA, US). Isopropyl alcohol (IPA, Catalog No. BDH2032-1GLP) was purchased from VWR (Radnor, PA, US). Hydrogen peroxide (H2O2 ...
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bioRxiv - Cancer Biology 2022Quote: ... HAF109) were incubated for 1 h prior to detection with SuperSignal West Pico Plus Chemisubstrate (VWR International, PIER34577).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... A 5 mL syringe was connected to a swivel using Tygon* tubing (AAQ04103; VWR; West Chester, PA, USA). Each swivel was coupled with a spring leash (C313C ...
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bioRxiv - Cell Biology 2020Quote: ... virus-containing supernatant was collected 48 h later and filtered through a 0.45 μm polyethersulfone filter (VWR 28145-505). For protein-coding constructs ...
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bioRxiv - Microbiology 2023Quote: ... The virus containing band was extracted from the gradient and desalted on a NAP column (VWR, Cytiva, Illustra NAP) by elution in PBS ...
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bioRxiv - Physiology 2020Quote: ... and decalcified in 10% EDTA overnight prior to embedding in VWR Clear Frozen Section Compound (VWR, West Chester, PA). Blocks were sectioned at 16 um at stained using rabbit-anti GFP Polyclonal Antibody (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... The supernatant (which contains the majority of virus) was filtered through a 0.2 μM PES filter (VWR, cat. #97066-214). 25 mL of 40% PEG8000 (JT Baker ...
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bioRxiv - Neuroscience 2020Quote: ... The olfactory epithelium was cryosectioned coronally into 16 μm -thick sections mounted on Superfrost Plus slides (VWR, West Chester, PA) coated with poly-D-lysine.
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bioRxiv - Developmental Biology 2022Quote: ... Blots were treated with SuperSignal West Pico PLUS chemiluminescent substrate (Thermo-Scientific) and visualized using the Azure Biosystems c600 or Kodak/Carestream BioMax Film (VWR).
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bioRxiv - Immunology 2019Quote: ... Cell monolayers were processed for ICC staining at 24 to 72 hpi depending on virus replication kinetics (as previously described [94]) or stained with Giemsa stain (VWR; 35086). Relative plaque titre was calculated as the plaque titre of a virus stock under the indicated condition divided by its titre under a control condition ...
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bioRxiv - Neuroscience 2020Quote: Samples were collected into 13 × 100 mm borosilicate tubes with a Teflon-lined cap (catalog #60827-453, VWR, West Chester, PA). Then 2 mL of CH3OH and 1 mL of CHCl3 were added along with the internal standard cocktail (250 pmoles of each species dissolved in a final total volume of 10 μl of ethanol:methanol:water 7:2:1) ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
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bioRxiv - Biochemistry 2022Quote: Samples were collected into 13 x 100 mm borosilicate tubes with a Teflon-lined cap (catalog #60827-453, VWR, West Chester, PA). After addition of standards ...
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bioRxiv - Cell Biology 2020Quote: ... Solutions were mixed by shaking the compound plate for 1 min at room temperature at 1200 rpm using a microplate shaker (cat. 12620-926, VWR, West Chester, PA). A typical plate layout map used for screening experiments is shown in Figure 1 ...
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bioRxiv - Cell Biology 2019Quote: Extraction of Lipids:IP beads were transferred into 13 x 100 mm borosilicate tubes with a Teflon-lined cap (catalog #60827-453, VWR, West Chester, PA) by addition of 1 mL CH3OH ...
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bioRxiv - Plant Biology 2020Quote: Leaf tissue was collected 7 days post infection, transferred to an extraction bag (Bioreba, Switzerland) and mixed with 5 ml of phosphate buffered saline (VWR, West Chester, PA), hand homogenized using a hand-held tissue homogenizer (Agdia ...
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bioRxiv - Microbiology 2021Quote: GC-376 (CAS: 1416992-39-6) was purchased from Enovation chemicals (Green book, NY) and dissolved in deionized water (HyClone, VWR, West Chester, PA) to the desired concentration.
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bioRxiv - Biochemistry 2022Quote: ... Protein bands were visualized using Blue BANDitTM protein stain (VWR Life Science, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227), and incubated at 100L°C for 10Lmin under reducing conditions ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... A 5 mL syringe mounted on the infusion pump was connected to a swivel coupled with a spring leash (C313C; Plastics One; Roanoke, VA, USA) and Tygon® tubing (AAQ04103; VWR; West Chester, PA, USA) suspended over the chamber’s ceiling on a balanced metal arm ...
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bioRxiv - Biophysics 2023Quote: ... The concentration of total proteins was determined with the BCA Protein Assay Kit (VWR International GmbH, Darmstadt, Germany). According to the total protein amount in each sample ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein was estimated using Bradford reagent (VWR).