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Citations for VWR :
1 - 50 of 1494 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... the purified and concentrated protein was incubated with 1:3 protein to biotin ratio of 1mM NHS-PEG4-Biotin (VWR #PI21362) for 1 hr at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... biotin from VWR (Radnor, PA).
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bioRxiv - Microbiology 2019Quote: ... Insoluble protein pellets were resolubilized in 3 M urea (VWR, Radnor, PA) and purified using immobilized metal affinity chromatography using Ni-NTA resin (Qiagen ...
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bioRxiv - Genetics 2022Quote: ... A nonspecific antibody binding was prevented by an incubation of the fixed embryos in 3% BSA (VWR Life Science, 422351S) in PBS-T (1h ...
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bioRxiv - Bioengineering 2020Quote: ... Then we denatured the phage protein coat by adding 7.5 ml of 3 M KOAc (VWR international), pH 5.5 ...
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bioRxiv - Plant Biology 2022Quote: ... For biotin depletion with PD-10 desalting columns (VWR; 17085101), the columns were equilibrated with SDT:water (1:5 ...
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bioRxiv - Microbiology 2021Quote: ... Free biotin was removed by Macrosep Advance Spin Filter 3K MWCO 20 mL (Cat no. 89131-974, VWR, USA). Aliquots of the samples were stored at −80°C for use as input in western blot ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein was estimated using Bradford reagent (VWR).
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bioRxiv - Bioengineering 2021Quote: ... 100 µM of D-(+)-biotin (VWR, Mississauga, Canada; Cat#97061-446) and bacterial cell extract overexpressing E.coli BirA as described 81 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein concentration was estimated by Bradford reagent (VWR) using bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2021Quote: ... Protein concentration was estimated by Bradford reagent (VWR) using bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2021Quote: ... The protein was incubated overnight with TEV (VWR) at 4°C and Histag and TEV was purified out with Ni-NTA beads (GE) ...
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bioRxiv - Immunology 2023Quote: ... 3% SDS (151-21-3, VWR Life Science) and 100 mM Tris–HCl [pH 6.8](1.00317 ...
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bioRxiv - Cell Biology 2020Quote: ... Residual lipids and proteins were cleared from the extracts by spin filtration in 3-kDa-molecular-weight spin cut-off filter (VWR) added 3 mg C18-material (Waters) ...
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bioRxiv - Cancer Biology 2021Quote: ... Endogenous peroxidase was blocked with 3% hydrogen peroxide (VWR, Lutterworth, UK) for 20 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Quenching of endogenous peroxidases was performed using 3% H2O2 solution (VWR). Slides were incubated with rabbit polyclonal UCP1 antibody (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were detected by immunoblotting using the following primary antibody concentrations in 5% milk (VWR)/Tris Buffered Saline -Tween20 (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... serum-free culture media containing secreted proteins were collected and concentrated in 0.5 mL 3-kDa molecular weight cut-off centrifugal filters (VWR North America). In some experiments ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: Protein extraction was performed with peqGOLD TriFast reagent (VWR), and proteins were separated by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (GE Healthcare Life Sciences) ...
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bioRxiv - Biochemistry 2023Quote: ... Staining was carried out with InstantBlue Protein Stain (VWR).
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined using the Bradford Assay (VWR). Protein lysates were diluted in RIPA buffer supplemented with 1× LDS and 1× DTT and heated at 95 °C for 5 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... 3% glucose (VWR), 3.5% active dried yeast (SLS) ...
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bioRxiv - Biophysics 2023Quote: ... The concentration of total proteins was determined with the BCA Protein Assay Kit (VWR International GmbH, Darmstadt, Germany). According to the total protein amount in each sample ...
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bioRxiv - Developmental Biology 2022Quote: ... The primary and the secondary antibodies were prepared in the blocking agent consisting of 0.1% Triton X-100 and 3% goat serum (VWR). Followed by washing the organoids with PBS 3x5min on a rocker ...
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bioRxiv - Microbiology 2021Quote: Protein lysate was collected with RIPA buffer (VWR Life Science) supplemented with 2X protease inhibitors (Pierce ...
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bioRxiv - Cell Biology 2019Quote: ... 0.45μm low protein binding membrane (#28145-479) was from VWR International ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant protein extracts were recovered by centrifugation and protein concentration in samples was determined using Bradford Reagent (VWR).
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bioRxiv - Cancer Biology 2023Quote: ... The intensity of DAPI was measured using FlexStation 3 Multimode Plate Reader (VWR).
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bioRxiv - Cancer Biology 2023Quote: ... 3 mM MgCl2 (VWR), 0.1% NP-40 substitute (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... Each fraction was resuspended in HEPES-NaCl buffer and 10 μL of each fraction was examined for protein concentration and lipid content using a Bradford protein assay (VWR) and the lipophilic FM4-64 dye (Molecular Probes) ...
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bioRxiv - Immunology 2020Quote: Cell protein was isolated using RIPA lysis buffer (VWR, Ontario, Canada) supplemented with protease inhibitor cocktail (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor growth was monitored weekly and tumor volume was measured along 3 axes with calipers (VWR 62379-531). Tumors and lungs were harvested at time of tumor burden (total tumor volume of 2cm3 ...
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bioRxiv - Biochemistry 2022Quote: ... The stirrer was an 8 mm x 3 mm pivot bar (VWR 37119-6183), stirring at 650 rpm ...
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bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
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bioRxiv - Synthetic Biology 2023Quote: ... ribosome was purified using hydrophobic chromatography (3×5 mL HiTrap Butyl HP column (VWR)) with Fast Performance Liquid Chromatography (FPLC ...
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bioRxiv - Neuroscience 2021Quote: ... The secondary antibody (donkey anti-rabbit IgG polyclonal antibody [horseradish peroxidase]; NA934) was from VWR International ...
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bioRxiv - Neuroscience 2024Quote: ... The secondary antibody (donkey anti-rabbit IgG polyclonal antibody [horseradish peroxidase]; NA934) was from VWR International ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein was isolated using Ni-NTA Resin (VWR Cat. No. 786-940) and eluted with 2 mL of elution buffer (300 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... A total amount of 15 μg protein was used for SDS-PAGE separation by electrophoresis and proteins were transferred onto nitrocellulose membranes (VWR, Lutterworth, UK). Even protein loading and transfer were confirmed by Revert 700 total protein staining (Li-COR ...
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bioRxiv - Cancer Biology 2019Quote: ... G418 (VWR, 970-3-058), for several passages ...
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bioRxiv - Neuroscience 2022Quote: ... 3% Acetonitrile (VWR Cat#83640.320), 1 μg of LysC (Wako Cat#129-02541) ...
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bioRxiv - Neuroscience 2023Quote: ... and 3% hydrogen peroxide (VWR) for 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... and 3 % of BSA (VWR) in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... fluorescence intensity (∼485 nm/∼530 nm) was measured using FlexStation 3 Multimode Plate Reader (VWR).
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bioRxiv - Cancer Biology 2023Quote: ... the upper part of the membrane was cleaned with cotton swab and lower part was stained with DAPI and the intensity of DAPI was measured using FlexStation 3 Multimode Plate Reader (VWR).
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bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred onto a 0.2μM nitrocellulose membrane (VWR: 27376-991). Membranes were blocked in 5% nonfat dairy milk in TBS-T ...
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bioRxiv - Biochemistry 2021Quote: ... The gel was stained with Ethidium Bromide and visualised using a Smart 3 GelDoc System (VWR).
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bioRxiv - Bioengineering 2020Quote: ... 1-(3-(Dimethylamino)propyl)-3-ethylcarboiimide hydrochloride (EDC-HCL; 0.5 g, 2.6 mmol, 1.02 equiv., VWR) and NHS-ester (0.65 g ...
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bioRxiv - Cell Biology 2023Quote: ... covered with a glass plate (3" x 3" glass plate from VWR Vertical Gel Electrophoresis Systems), exposed to 7.5 minutes of glass plate filtered (UVA only ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mM MnCl2 (VWR; Cat. # 1.05927.1000), 3 µM Na- orthovanadate (Sigma ...