Labshake search
Citations for VWR :
1 - 50 of 90 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... cDNA libraries were generated by incubating the RNA samples in a solution containing qScript cDNA SuperMix (VWR #101414-102) and nuclease-free water ...
-
bioRxiv - Cell Biology 2022Quote: ... two washes with Histoclear (VWR) and embedding in Permount (Thermo Fisher) ...
-
bioRxiv - Cell Biology 2023Quote: ... DNA-RNA hybrids were eluted and treated with Proteinase K (VWR Scientific). Eluted DNA was subsequently isolated by phenol:chloroform:isoamyl alcohol extraction and DRIP-qPCR was performed on Myocardin promoter and control locus and results were calculated as per Sanz and Chédin ...
-
bioRxiv - Physiology 2020Quote: ... two of which act as air holes and two hold the glass capillaries (VWR 53432-706). CAFE chambers were made from standard fly vials (25mm x 95mm ...
-
bioRxiv - Neuroscience 2020Quote: ... between two coverslips (VWR Cat #84393-251). Hair cells were counted in four neuromasts (SO1 ...
-
bioRxiv - Physiology 2021Quote: ... and two calibrated glass micropipettes (5μL, VWR) filled with liquid medium (5% sucrose + 5% autolysed yeast extract ...
-
bioRxiv - Plant Biology 2019Quote: ... two strips of micropore tape (56222-182, VWR) were placed across the jar opening ...
-
bioRxiv - Microbiology 2023Quote: ... Two pieces of lab tape (VWR #89098-074) were placed on top of one another ...
-
bioRxiv - Physiology 2023Quote: ... Two 5 µl calibrated capillary tubes (VWR International) filled with 10% sucrose containing CaCl2 of various concentrations (see 2.2.1 ...
-
bioRxiv - Developmental Biology 2020Quote: ... placed between two #1.5 coverslips (VWR, cat #16004-312) separated by a rubber spacer (Grace Bio Labs ...
-
bioRxiv - Biochemistry 2023Quote: ... followed by two additional chloroform extractions (VWR, 97064-680) and ethanol precipitated ...
-
bioRxiv - Cell Biology 2022Quote: ... cDNA was prepared using QScript cDNA Supermix (VWR cat # 101414-106) and analyzed for gene expression using quantitative real-time PCR with iQ SYBR Green Supermix (Biorad cat # 1708885).
-
bioRxiv - Developmental Biology 2022Quote: ... connected to two syringes via rubber tubings (VWR, Tygon 3603). One tube can be closed by by a two-way manual valve (Masterflex ...
-
bioRxiv - Genomics 2023Quote: ... followed by two washes with freshly made 80% ethanol (VWR), drying 3-5 min and elution in 25 μl water ...
-
bioRxiv - Genomics 2023Quote: ... followed by two washes with freshly made 80% ethanol (VWR), drying 3-5 min and elution in 22 μl water ...
-
bioRxiv - Genomics 2023Quote: ... followed by two washes with freshly made 80% ethanol (VWR), drying 3-5 min and elution in 32 μl molecular biology grade water (Cytiva) ...
-
bioRxiv - Biophysics 2023Quote: ... nuclei were sandwiched between two coverslips (631-1573P, VWR International) at different time points ...
-
bioRxiv - Genomics 2022Quote: ... and reverse transcribed to cDNA using qScript™ XLT cDNA SuperMix (VWR) or iScript cDNA Synthesis Kit (1708891 ...
-
bioRxiv - Cancer Biology 2023Quote: cDNA was prepared with qScript™ cDNA SuperMix (Cat: 101414-106, VWR) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... Cleared embryos were placed between two coverslips (VWR cat # 16004-312) separated by FastWells spacers (Grace Bio-Labs ...
-
bioRxiv - Genetics 2023Quote: ... and two 2.4 mm metal beads (VWR 500g x 2.4mm Metal). The microcentrifuge tubes were placed in a Mini-BeadBeater-96 homogenizer (Biospec ...
-
bioRxiv - Biophysics 2023Quote: ... we built a chamber by assembling two silanized coverslips (VWR, Germany) on the glass spacers cut out of a coverslip (thickness ≈ 0.140 mm) ...
-
bioRxiv - Genetics 2021Quote: ... then reverse transcribed into cDNA using Quanta Biosciences qScript cDNA mix (VWR International). qPCR was performed in duplicate using Quanta Biosciences Perfecta qPCR Supermix (VWR International ...
-
bioRxiv - Genetics 2019Quote: ... then reverse transcribed into cDNA using Quanta Biosciences qScript cDNA mix (VWR International). qPCR for Gli1 was performed using Quanta Biosciences Perfecta qPCR Supermix (VWR International ...
-
bioRxiv - Biophysics 2021Quote: We prepared containers with two microscope glass slides (VWR, Radnor, Pennsylvania, USA), separated by two bands of paraffin ...
-
bioRxiv - Cancer Biology 2023Quote: ... using two injections of 10 mM Glycine-HCl (VWR LifeSciences, Radnor, PA) and one injection of 350 mM EDTA (TCI America ...
-
bioRxiv - Microbiology 2023Quote: ... We fabricated the millifluidic devices using two 1mm thick microscope slides (VWR, Vistavision Microscope Slide ...
-
bioRxiv - Cell Biology 2024Quote: ... and 1 μg was converted to cDNA using the qScript cDNA Synthesis Kit (VWR 101414). Technical replicates of 20 ng (based on RNA concentration ...
-
bioRxiv - Biophysics 2022Quote: An open experimental chamber was formed by using two clean glass coverslips (VWR) separated by 1 mm on a custom-built chamber holder ...
-
bioRxiv - Neuroscience 2021Quote: ... The dissected cuticle was sandwiched between two #1 coverslips (VWR #1 16004-094) with ~10 μL of UV curable resin (Bondic UV glue #SK8024 ...
-
bioRxiv - Plant Biology 2023Quote: ... Supernatant was pipetted out to a two-sided disposable plastic cuvettes (VWR, 97000-586) for chlorophyll (Chl ...
-
bioRxiv - Immunology 2023Quote: ... Bacterial density was measured at OD600 every hour in two-sided polystyrene cuvettes (VWR) using a SpectraMax M3 Spectrophotometer (Molecular Device) ...
-
bioRxiv - Developmental Biology 2023Quote: ... The 3’ end enriched libraries were constructed using KAPA HyperPlus Library Preparation Kit (VWR International, KK8513) with 6 cycles of PCR ...
-
bioRxiv - Genomics 2020Quote: ... The sample was dounced (two strokes) in a 14-ml stainless steel tissue grinder (VWR) then spun at 100 g for 6 min to pellet remaining worm fragments ...
-
bioRxiv - Genomics 2020Quote: ... two million cells were resuspended in 100 μl cold BTXpress electroporation solution (VWR 732-1285) with 20 μg Cas9 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... all growths at the latter two temperatures were carried out in an incubator (VWR International) in the dark over four days ...
-
bioRxiv - Biophysics 2021Quote: ... Two no.1 cover slides (24 × 60 mm and 18×18mm, VWR, Radnor, Pennsylvania, USA) are cleaned by sonication in isopropanol and dried under a stream of N22 ...
-
bioRxiv - Biophysics 2022Quote: ... thin sections of each were sandwiched between two glass coverslips (VWR, Cat. No 631-0165). In order to reduce the RI mismatch between the samples and surrounding media ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... all growth at the latter two temperatures was carried out in an incubator (VWR International). The oatmeal cultures were prepared in 250 ml Erlenmeyer flasks that contained 10 g of autoclaved oatmeal (10 g of oatmeal with 17 ml of deionized H2O and sterilized for 15–20 min at 121 °C) ...
-
bioRxiv - Neuroscience 2024Quote: ... The stimulating electrode was paired with two remote 2x2mm Ag/AgCl electrodes (VWR, Lutterworth, UK) in a y-shape configuration ...
-
bioRxiv - Microbiology 2024Quote: Shotgun sequencing libraries were prepared using the Lucigen NxSeq AmpFREE Low DNA Library Kit (VWR International, Radnor, USA), and sequenced with PE150 on an Illumina NovaSeq6000.
-
bioRxiv - Molecular Biology 2022Quote: ... 500 ng of RNA was reverse transcribed by oligo-dT and random priming into cDNA with a qScript cDNA SuperMix kit (QuantaBio-VWR), according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1ug of RNA was reverse transcribed by oligo-dT and random priming into cDNA with a qScript cDNA SuperMix kit (QuantaBio-VWR), according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2021Quote: ... 1µg of RNA was reverse transcribed by oligo-dT and random priming into cDNA with a qScript cDNA SuperMix kit (QuantaBio-VWR), according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... Approximately 250-500ng RNA was normalized across samples and cDNA was generated using the qScript cDNA Synthesis Kit (101414-098, VWR). cDNA was then subjected to qPCR using PerfecTa SYBR Green FastMix (84069 ...
-
bioRxiv - Microbiology 2020Quote: ... The mixture was gently spread on two parallel SM/5 plates using a plastic rake (VWR), resulting in technical replicates ...
-
bioRxiv - Genetics 2020Quote: HCT116 cells were transfected with px459sgCTNNB1 and the two donor template encoding plasmids KOZAK_FLAG_mClover3 and KOZAK_V5_mCherry using TransIT (731-0029; VWR) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... a two-fold serial dilution with 1X reaction buffer was conducted in clear PCR tubes (VWR). The methyltransferase reaction was initiated by adding 10 ml of 2x substrate solution (80 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2019Quote: ... Pads were cast by depositing 500 µL of molten agarose between two 25 × 25 mm coverslips (VWR) that were rinsed with ethanol and deionized (DI ...
-
bioRxiv - Cell Biology 2020Quote: ... two vials were thawed and cells were seeded overnight onto 150mm cell culture dishes (VWR, 734-2322) in 18ml R10 and 2ml GM-CSF ...