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Citations for VWR :
1 - 50 of 61 citations for Smooth Muscle Actin Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Fascia of TA muscles was removed and muscles frozen in liquid nitrogen-cooled isopentane (VWR) and placed at −80°C until cut ...
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bioRxiv - Molecular Biology 2022Quote: ... Muscle was homogenized in Trizol (VWR; Radnor, PA, USA) with tight fitting plastic pestles for approximately 30 seconds and stored in −80°C overnight to increase RNA yield ...
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bioRxiv - Molecular Biology 2021Quote: ... Contralateral muscles were embedded in optimal cutting temperature medium (VWR, UK) and subsequently frozen in liquid nitrogen-cooled isopentane (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... The antimicrobial peptide C18G (VWR) was added at 5 µg/mL ...
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bioRxiv - Cell Biology 2020Quote: Tibialis anterior muscles were homogenized in BioMasher homogenization tubes (VWR KT749625-0030) in RIPA lysis buffer (VWR 97063-270 ...
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bioRxiv - Physiology 2021Quote: Muscles were lysed in lysis buffer50 using a bead beating homogenizer (VWR, 10158-558) with 100x protease and phosphatase inhibitor cocktail (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from muscles using Ribozol (Cat. No CA97064-950, VWR international) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Muscle tissues were harvested from sacrificed mice and fixed in 10% formalin (VWR, 11699404). The fixed tissues were dehydrated ...
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bioRxiv - Molecular Biology 2019Quote: ... After blocking with 5% BSA (VWR LIFE SCIENCE) for 1 h at room temperature ...
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bioRxiv - Physiology 2020Quote: TA muscles were mounted on piece of cork and fixed with tragacanth gum (VWR, 24437.260) before being frozen in isopentane cooled by liquid nitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... muscle fibres were seeded in a thin layer matrigel (VWR, Cat#734-0269, Radnor, PA, USA)-coated 6-well plate containing DMEM growth medium ...
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bioRxiv - Pathology 2021Quote: ... After blocking with 3% bovine serum albumin (BSA, VWR Chemicals, UK) in TBS-tween ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by blocking by 3% bovine serum albumin (BSA, VWR, 421501J) in phosphate-buffered saline-Tween 0,1% (PBS-T 0,1%) ...
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bioRxiv - Immunology 2021Quote: ... Slices were stained in a blocking buffer composed of TritonX100 (VWR) 0.1-0.3 % ...
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bioRxiv - Genetics 2024Quote: ... The DNA was isolated from frozen ear muscle biopsies using the peqGOLD blood & tissue DNA mini kit (VWR) and following the protocol provided by the manufacturer.
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bioRxiv - Pathology 2019Quote: ... After blocking with 1% bovine serum albumin (BSA) (VWR, Radnor, PA, US), the membranes were probed with 1 μg/mL mAb 9G3 against the glycosylated epitope ‘KEPAPTTT’ in the lubricin mucin domain (Merck KGaA ...
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bioRxiv - Biophysics 2022Quote: Muscle tissue culture molds were fabricated from polymethyl methacrylate (PMMA) and glued onto a microscopy cover glass (VWR, Radnor, USA) as previously described[22] ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked with the LI-COR Biosciences Intercept Blocking buffer (VWR International) for one hour at room temperature and incubated with primary antibodies overnight at 4 °C on a rocker ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with blocking solution PTWH (0.2% Tween-20 (Cat. #: 28829.183, VWR) and 10 mg/L heparin (cat ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were blocked at room temperature for 1 h using Rockland blocking buffer (VWR). Primary PDI antibody (Abcam ...
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bioRxiv - Developmental Biology 2020Quote: ... for blocking and with 0,2% Tween® 20 added for antibody dilutions (28829.296, VWR), washes were done with phosphate-buffered saline tween (PBST ...
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bioRxiv - Immunology 2020Quote: ... Plates were then blocked with 100μl of blocking buffer (1% Casein in PBS; VWR) for 1.5hr at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with a blocking solution (2 % bovine serum albumin (BSA; VWR) diluted in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... After blocking with 5% bovine serum albumin (BSA; VWR Life Science, Radnor, PA, USA) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were blocked for 1 h with Rockland blocking buffer (VWR, Catalog #RLMB-070) then incubated with PDI antibody (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated in blocking buffer consisting of 5% bovine serum albumin (BSA; VWR) dissolved in 1X Tris-buffered saline (TBS ...
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bioRxiv - Neuroscience 2023Quote: ... peptides were dissolved in titanium dioxide (TiO2) loading buffer (80% acetonitrile (ACN, VWR), 5% Trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2021Quote: ... Blocking buffer and primary antibody incubation solution was 5% non-fat dairy milk powder (VWR) or 5% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Phosphorylated peptides were eluted twice using 150 µl 300 mM ammonium hydroxide (VWR, 1.05432.1000), eluates were united and immediately acidified with conc ...
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bioRxiv - Molecular Biology 2023Quote: ... Phosphorylated peptides were eluted twice using 150 µl 300 mM ammonium hydroxide (VWR, 1.05432.1000), eluates were united and immediately acidified with conc ...
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bioRxiv - Cell Biology 2021Quote: ... and LiCOR Intercept Tris buffered saline (TBS) blocking buffer (Catalog # 103749-018) were purchased from VWR/Avantor ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were probed with mouse anti-α-tubulin antibody (DM1A, VWR; #PI62204; 1:1000 in blocking buffer) for 1h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Membrane blocking was performed for 1 h at room temperature in 5% bovine serum albumin (BSA; VWR) in Tris-buffered saline containing Tween-20 (TBST) ...
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bioRxiv - Cancer Biology 2023Quote: ... the peptides were re-dissolved in 10 μl 1% formic acid (VWR Ltd., Radnor, PA, USA) and were separated in a 180 min water/acetonitrile gradient using an Easy nLC 1200 nano UPLC (Thermo Scientific ...
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bioRxiv - Genomics 2019Quote: ... Western blots were performed using a mixture of mouse anti-actin antibodies (VWR catalog number 89500-294) and rabbit anti-TAP antibodies (ThermoFisher catalog number CAB1001 ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a 60-minute blocking with donkey serum (1:10 diluted in 1% BSA; VWR, Pennsylvania, USA). Incubation with the primary antibody was performed overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... and then incubated with the primary antibody diluted in Intercept Blocking buffer supplemented with 0.1 % Tween20 (Carl Roth, 9127.1) and 0.2 % sodium dodecyl sulfate (SDS; VWR, A3942) for 16 h at 4° C ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was incubated in blocking solution [5% milk in PBST (PBS (pH 7.4) with 0.1% Tween-20 (VWR))] for 2 h and probed overnight with the following primary antibodies ...
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bioRxiv - Bioengineering 2023Quote: ... primary antibodies were added to the blocking solution for 20 minutes while rocking (VWR analog rocker 10127-876), in the dark and on ice ...
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bioRxiv - Cell Biology 2021Quote: ... All washing steps between blocking or chemical incubation were performed with PBS supplemented with 0.5 % Tween-20 (VWR, 8.22184).
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bioRxiv - Developmental Biology 2022Quote: ... The primary and the secondary antibodies were prepared in the blocking agent consisting of 0.1% Triton X-100 and 3% goat serum (VWR). Followed by washing the organoids with PBS 3x5min on a rocker ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were blocked for 90 minutes at room temperature in a humidified chamber with a blocking buffer consisting of 0.1% Triton X-100 and 5% Bovine Serum Albumin (VWR). All the antibodies used in this study and their concentrations are listed in File S6 ...
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bioRxiv - Developmental Biology 2022Quote: ... Later blocking was done for 1hour at RT on a rocker with a blocking buffer consisting of 0.1% Triton X-100 and 5% goat serum (VWR). The organoids were incubated overnight in the primary antibodies (File S6 ...
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bioRxiv - Developmental Biology 2022Quote: ... Later blocking was done for 1hour at RT in a humidified chamber with a blocking buffer consisting of 0.1% Triton X-100 and 5% Bovine Serum Albumin (VWR). The organoids were incubated in primary antibodies (File S6 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was washed 3 times in PBS before blocking for 30 minutes room temperature with PBS+0.1% Triton X-100 (VWR) with 5% donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated for 1-2 hours at RT with primary antibodies diluted in Odyssey Blocking Buffer + 0.2% Tween (VWR). Next ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µl of a blocking/permeabilization buffer (5% BSA and 0.2% Triton in PBS; BSA from Sigma; TritonX-100 from VWR) was added and cells were incubated for 1h at RT ...
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bioRxiv - Physiology 2021Quote: ... 200 µl of these fixed RBCs were re-suspended in a blocking buffer containing 3% bovine serum albumin (VWR 0332) and 1% normal goat serum (Lampire Biological Laboratories 7332500 ...
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bioRxiv - Cell Biology 2022Quote: B16-F1 cells were transfected with 0.5 µg EGFP-β-actin and 1 µL JetPrime transfection reagent (VWR, #114-07) per well in 6-well plates according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Peptides were solubilized in 6 μL buffer A (100% MS-LC grade water and 0.1% Formic acid (VWR Cat#84865.180), and a total volume of 3 μL were loaded onto a 30 cm-long column (75 μm inner diameter (Polymicro Cat#TSP075375) ...