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Citations for VWR :
1 - 50 of 190 citations for SDS PAGE Single gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Samples were analyzed using standard SDS-PAGE (4-16% bis acrylamide, Precast gels, VWR) and immunoblotting techniques ...
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bioRxiv - Biophysics 2019Quote: ... The reactions were quenched by the addition of SDS-PAGE loading buffer and analyzed by 10% Tricine-SDS-PAGE and stained with Coomassie Brilliant Blue G-250 (VWR).
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bioRxiv - Plant Biology 2021Quote: ... The samples were prepared according to the manufacturer’s instructions and proteins were separated in 10 % Next Gel SDS-PAGE (VWR). Carbonylated proteins were detected with Immobilon Western Chemiluminescent HRP Substrate (MilliporeSigma).
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bioRxiv - Microbiology 2021Quote: ... 10 μl of the cell lysates were resolved by standard 10% SDS-PAGE gel and electroblotted onto 0.2 mm nitrocellulose membrane (Biotrace, VWR) using a semi-dry transfer unit (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... under denaturating conditions (SDS PAGE Sample loading buffer (VWR, GENO786-701) supplemented with 1 mM DTT ...
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bioRxiv - Cancer Biology 2021Quote: ... under denaturating conditions (SDS PAGE Sample loading buffer (VWR, GENO786-701) supplemented with 1 mM DTT) ...
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bioRxiv - Cell Biology 2022Quote: ... under denaturating conditions (SDS PAGE sample loading buffer (VWR, GENO786-701) supplemented with 1 mM DTT ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were separated by SDS-PAGE and transferred to PVDF (VWR). Blots were rinsed once with TBST then blocked in 5% milk at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... or 10% SDS-PAGE and wet transferred to Nitrocellulose membranes (VWR, CA10061-084) at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were resolved by 8% SDS-PAGE and transferred to nitrocellulose membranes (pore 0.2μm, VWR #27376-991). Membranes were blocked in TBS-Tween (25mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... pEV and plasma and cell lysates as positive controls were loaded on a 12% SDS-PAGE in loading buffer (50 mM Tris-HCl (pH 8.0)(M151, VWR), 150 mM NaCl (S7653 ...
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bioRxiv - Genetics 2019Quote: ... Eluates were analysed by SDS-PAGE followed by western blotting with antibodies anti-V5 tag (VWR, MEDMMM-0168-P), anti-Pgk1 (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... Peak fractions were analyzed using SDS-PAGE and were further purified in a Superose 6 Increase 10/300 (VWR) using Buffer A as running buffer (free of EDTA/PMSF) ...
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bioRxiv - Plant Biology 2021Quote: ... Membrane protein samples containing 1 μg total Chl were solubilized and separated by electrophoresis on a 10 % SDS-polyacrylamide gel using Next Gel solutions and buffers (VWR, Radnor, PA, USA). Proteins were transferred to Immobilon-P PVDF membranes (MilliporeSigma ...
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bioRxiv - Cell Biology 2020Quote: Protein extracts from total cells and eluates from immuno-precipitations were separated by standard SDS-PAGE and transferred to PVDF membranes (#10600023, VWR, USA) for the detection of Mup1-GFP ...
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bioRxiv - Cell Biology 2021Quote: ... 40% glycerol and 412mM β-mercaptoethanol) before being resolved by 8% SDS-PAGE and transferred to nitrocellulose membranes (pore 0.2μm, VWR #27376-991). Membranes were blocked in TBS-Tween (25mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... Each sample (10 μL) was loaded and separated on an SDS-PAGE followed by Coomassie G-250 staining (VWR, Cat. # 0615) to visualize the overexpression of the desired fusion protein.
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bioRxiv - Cancer Biology 2023Quote: ... and 412 mM β-mercaptoethanol) and resolved by SDS-PAGE followed by transfer to nitrocellulose membranes (pore 0.2μm, VWR #27376-991). Membranes were blocked in TBS-Tween (25 mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... A total amount of 15 μg protein was used for SDS-PAGE separation by electrophoresis and proteins were transferred onto nitrocellulose membranes (VWR, Lutterworth, UK). Even protein loading and transfer were confirmed by Revert 700 total protein staining (Li-COR ...
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bioRxiv - Cell Biology 2022Quote: ... Samples containing 20-50 µg protein were separated by SDS polyacrylamide gel electrophoresis and transferred to a nitrocellulose or polyvinylidene fluoride membrane (10600023, VWR, Radnor, PA). After blocking the membrane for 1h in 3% BSA in Tris- buffered saline with Tween 20 (TBS-T) ...
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bioRxiv - Biochemistry 2023Quote: ... 10% SDS (VWR LifeSciences), 0.04% bromophenol blue (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... 4% SDS (VWR #442444H), 160 mM Tris-HCl pH 6.8 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The active fractions from the NC-MT assay were separated in a 10% SDS gel and stained with coomassie blue (InstantBlue, VWR Chemicals, Darmstadt, Germany) or silver stain kit for mass spectrometry (Pierce/Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% SDS) plus Proteinase K (390973P; VWR), and incubated at 55°C overnight ...
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bioRxiv - Bioengineering 2022Quote: ... 0.02% SDS with Protease Inhibitor Cocktail (VWR)] and protein concentration was determined using the DC Protein Assay Kit (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... and only 0.25 % (w/v) SDS (VWR, UK) was found to remove the bound protein ...
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bioRxiv - Biochemistry 2020Quote: ... and only 0.25 % (w/v) SDS (VWR, UK) was found to remove the bound protein ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and 25 μl 10% SDS (VWR Life Science). The mixture was vortexed vigorously and incubated at 55 °C for 60 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... and only 0.25 % (w/v) SDS (VWR, UK) was found to remove the bound protein ...
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bioRxiv - Biophysics 2022Quote: ... Sodium dodecyl sulfate (SDS, 0227, VWR Life science), Sodium chloride (NaCl ...
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bioRxiv - Immunology 2023Quote: ... 3% SDS (151-21-3, VWR Life Science) and 100 mM Tris–HCl [pH 6.8](1.00317 ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... single cell suspension was prepared using Percoll (VWR, Radnor, PA) and red blood cells were lysed with ACK lysis buffer (Life Technologies) ...
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bioRxiv - Plant Biology 2022Quote: ... Amplicons were separated using agarose gel electrophoresis with mini Gel II (VWR), stained with Midori Green (MG 04 ...
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bioRxiv - Biochemistry 2020Quote: ... The Click reaction contained (final concentrations) 1% SDS (VWR), 5% t-butanol (Fisher) ...
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bioRxiv - Genomics 2023Quote: ... cells were disrupted with sodium dodecyl sulphate (SDS) (VWR) at 65 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... single-stranded RiboReady 1 kB RNA ladder (VWR Life Sciences, USA). Internally labelled RNA was similarly created using in vitro transcription ...
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bioRxiv - Neuroscience 2021Quote: ... Gel electrophoresis was performed with 1.8% agarose gels (VWR Life science, Sigma- Aldrich) in 1X TAE buffer (40 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA quality was examined via gel electrophoresis on a 0.5% agarose gel (VWR) with 0.1% GelRed (10000x ...
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bioRxiv - Genetics 2019Quote: ... DNA was extracted from the gel slices using the peqGOLD Gel Extraction Kit (VWR). Genotyping was done using a modified ast-PCR protocol for R21X (Supplemental methods ...
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bioRxiv - Biochemistry 2023Quote: • 20 % (w/v) SDS (VWR, USA; cat. no. 97062-442)
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bioRxiv - Microbiology 2021Quote: ... Plates were closed and sealed with a single layer of Parafilm (VWR) to allow for water containment and gas permeability ...
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bioRxiv - Bioengineering 2023Quote: Single cell suspensions were prepared from clusters incubation in trypsin/EDTA (VWR). 20% of the total volume of fetal bovine serum was added and the tube placed on ice ...
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bioRxiv - Biochemistry 2024Quote: Single-molecule experiments were conducted in chambers prepared from glass coverslips (VWR) and microscope slides (Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... resuspended in PBS + 2% SDS + protease inhibitor cocktail (VWR, #97063-972), and transferred to 2 mL disruption tubes (OPS Diagnostics ...
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bioRxiv - Bioengineering 2023Quote: ... 0.02% SDS with Protease Inhibitor Cocktails (VWR Life Science, 97063-010)] ...
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bioRxiv - Developmental Biology 2021Quote: ... a single specimen was suspended in undiluted Matrigel (VWR International GmbH; 734-1101) to recapitulate the in-vivo microenvironment ...
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bioRxiv - Developmental Biology 2021Quote: ... dissociated into a single-cell suspension and filtered through a 40μm cell strainer (VWR). FACS analysis and cell sorting were performed using Fortessa and AriaIII instruments (Becton Dickinson) ...
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bioRxiv - Biophysics 2022Quote: Single-molecule experiments were conducted using custom flow cells prepared from glass coverslips (VWR) and slides (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed with 1% sodium dodecyl sulfate (SDS) in PBS (VWR) and sonicated at 50% amplitude for 10 seconds twice ...
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bioRxiv - Biochemistry 2020Quote: ... Silica gel 40-60 μm from VWR International was used ...