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Citations for VWR :
1 - 50 of 1212 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Sf9 insect cells (VWR, #EM71104-3) were maintained in ExCell 420 serum-free media (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Resistance selection using fixed concentrations of COV-siRNA1 or COV-siRNA1+2 was conducted in Vero E6 cells cultured in DMEM supplemented with 10% FBS (VWR) and 1x Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Evolutionary Biology 2023Quote: ... containing 6-His tag and TEV cleaving site.19 In 50 mM HEPES (VWR, PA, USA), pH 7.5 including 50 mM potassium sulfate (Honeywell ...
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bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were resuspended in 50μl Schneider’s Insect Medium and transferred onto chambered cell culture slides (VWR, 53106-306). The chamber slides were pretreated with 0.25mg/ml Concanavalin A (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: Drosophila melanogaster S2R+ cells were maintained in Schneider’s Insect Medium supplemented with 10% heat inactivated FBS (VWR) and 1% Penicillin-Streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... anti V5-tag (VWR, MEDMMM-) and Anti-pgk1(Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL of each rescuer strain was inoculated into 500 μL of SC -his media in 96 well 2 mL deep well plate format (VWR) and shaken at 900 rpm at 30°C ...
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bioRxiv - Microbiology 2023Quote: ... infectivity and spread cells were stained with a SARS-CoV-2 anti-N rabbit polyclonal antibody for 1 hour followed by 594-conjugated secondary Goat anti-Rabbit IgG (VWR, GtxRb-003-D594NHSX) for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... His-bind resin was purchased from VWR.
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bioRxiv - Developmental Biology 2022Quote: ... Ovaries were dissected from 40 flies in Complete medium (Grace’s Insect Basal medium (VWR; #45000-476) supplemented with 15% FBS (ATCC ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 2% formaldehyde (VWR). Samples were run on a Novoctye 3000 (ACEA Biosciences ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed in 2% formaldehyde (VWR) and analysed using an LSR Fortessa analyser (BD ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Developmental Biology 2022Quote: ... tjTS>lglRNAi ovaries (with no UAS-tagged fluorescence reporter) were dissected in Grace’s Insect Basal medium (VWR; #45000-476), and incubated in Dihydroethidium (Invitrogen ...
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bioRxiv - Plant Biology 2019Quote: 6x-His purification was conducted in 20 mL gravity columns (VWR) loaded with 4 mL HisPur™ Ni-NTA resin (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... mouse anti-HIS his.h8 monoclonal (1:1000 dilution, VWR #101981-852); goat HRP-anti-rabbit and goat HRP-anti-mouse (1:1000 dilutions ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed in 2% paraformaldehyde (VWR International, Inc.) to assess pan-immune cells in Fig ...
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bioRxiv - Molecular Biology 2021Quote: 2 x 107 BCBL-1 cells per time point were crosslinked with 2% formaldehyde (VWR M134) in prewarmed 1X phosphate buffered saline (PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Cryosections (10 µm thickness) were collected on His-bond glass slides (VWR) and processed following (Jacobson et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10% fetal bovine serum (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... cells were plated on No.2 VistaVision cover glasses (VWR). For hydraulic chamber experiments and immunofluorescence ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were selected with 2 μg/ml puromycin (VWR, CAYM13884) for five days and subsequently split into the different screening condition arms ...
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bioRxiv - Immunology 2022Quote: 3 µg/ml of spike antigens were coated onto the 384-well ELISA plates (VWR, Cat # 82051-300) overnight at 4 degree ...
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bioRxiv - Microbiology 2024Quote: Cells from bone marrow of 6-10-week-old male or female wild-type or Myd88-/- Ticam-/- Mavs-/- (49) C57BL/6 mice (see Table S1) were cultivated in RPMI 1640 (VWR) with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2021Quote: ... 2 million cells were seeded into a 10 cm dish (VWR) and transfected with 8 μg hASIC1a WT ...
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bioRxiv - Microbiology 2024Quote: Caco-2 cells were seeded into 24-well Transwell plates (VWR) at a density of 1.5×105 cells/cm2 and grown at 37°C in 5% CO2 for 14 or 21 days for confluency ...
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bioRxiv - Cancer Biology 2019Quote: ... 1-2 × 105 cells were plated in transwell inserts (VWR, Radnor, PA) coated with 0.3 mg/mL Corning™ Matrigel™ Membrane Matrix (Corning ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were permeabilized and blocked in 2% bovine serum albumin (BSA) (VWR) and 0.1% triton X (VWR ...
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bioRxiv - Molecular Biology 2020Quote: ... or a non-specific siGENOME Non-Targeting siRNA (Pool #2, Dharmacon) were transfected into U-2 OS cells with INTERFERin™ Polyplus Reagent (VWR) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 100 μg/mL of 5-florocystosine (5-FC) and grown at 24°C as listed in Supplemental Table S1 (Stork et al. 2008) (VWR, Radnor, PA, USA). A 10 mg/mL stock solution of 5-FC was prepared in distilled water and filtered sterilized ...
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bioRxiv - Cell Biology 2021Quote: ... The stable cell lines were plated at 2×106cells/ 35mm cell culture dish (VWR, Radnor, PA, cat # 82050-538). After 24hrs ...
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bioRxiv - Neuroscience 2023Quote: Primary microglial cells were seeded at a density of 2 x 105 cells on 12 mm glass coverslips (VWR) coated with poly-L-lysine (0.1 mg/mL) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with a blocking solution (2 % bovine serum albumin (BSA; VWR) diluted in PBS ...
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bioRxiv - Immunology 2023Quote: MDCK.2 cells (ATCC) were seeded at a density of 1 × 106 cells/well in 6-well plates (VWR International) and incubated overnight at 37℃ 5% CO2 ...
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bioRxiv - Microbiology 2019Quote: ... colonies were pooled by suspending the cells in 2 mL 0.9% NaCl per plate with a cell scraper (Corning®, VWR, Germany).
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bioRxiv - Cell Biology 2022Quote: ... were grown to O.D 0.3-0.6 and incubated with non-fluorescent Halo tag ligand 7-bromo-1-heptanol (7BRO, 100μM, VWR #AAH54762) for 10 min to irreversibly mask all the pre-existing ribosomal proteins ...
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bioRxiv - Microbiology 2019Quote: ... 2 mL of cells were transferred to 4.5 mL disposable cuvettes (VWR Cat# 30620-276). Ethidium bromide (6.25 μM ...
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bioRxiv - Microbiology 2020Quote: ... were co-transfected into cells at a ratio of 3:2:1 using PEI (VWR). After 16 h incubation ...
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bioRxiv - Bioengineering 2019Quote: ... fixed cells were incubated in 2% (w/v) BSA+0,1% (v/v) triton X (VWR) in PBS ...
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bioRxiv - Microbiology 2020Quote: ... 2 mL of cells were transferred to 4.5 mL disposable cuvettes (VWR Cat# 30620-276) Ethidium bromide (6.25 μM ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cell/DNA mix was then transferred to a pre-chilled 2 mm cuvette (VWR) and pulsed in a GenePulser Xcell (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: Plated cells were fixed for 10 minutes with 2% paraformaldehyde (PFA; VWR Chemicals, Cat# 28794.364) in phosphate buffer and washed for 3×5 minutes with PBS ...
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bioRxiv - Neuroscience 2022Quote: ... or 2% BSA (2% Bovine Serum Albumin (VWR), 50mM Tris-Cl ...
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bioRxiv - Bioengineering 2021Quote: ... The cells were plated to ∼2 x 105 cells per well in a 6-well VWR tissue culture-treated plates (VWR, Suwanee, GA) and incubated ∼12 hours (overnight) ...