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Citations for VWR :
1 - 50 of 272 citations for Rnase 3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
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bioRxiv - Neuroscience 2019Quote: HEK293 cells were plated onto 12-mm glass coverslips (VWR) in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells were plated on sterilised 24 mm coverslips (VWR) at a low density to allow for single cells to be measured and clear background regions were available to record ...
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bioRxiv - Molecular Biology 2022Quote: HCT116 and HEK293 cells were seeded on 1.5mm coverslips (VWR) 24 hours prior to transfection with pLEICS25 wild-type or mutant GFP-Sam68 ...
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bioRxiv - Cell Biology 2021Quote: ... After 1h RNAse (VWR) and 2h Proteinase K (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... 3 minutes), once with 2X SSC (room temperature, 3 minutes) and then treated with 50 ug/mL of RNase A (VWR 1247C393) in 2X SSC for 45 minutes at 37°C by overlaying the sample with 500ul of RNAse solution and incubating in a humidified chamber ...
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bioRxiv - Biophysics 2022Quote: ... His-bind resin was purchased from VWR.
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bioRxiv - Genomics 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 65 μL RNase A (VWR #97062-172) solution (2 mg/mL RNase A in 10 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2020Quote: ... in 96 well plates (VWR, DNase, RNase free) containing 2 μL of lysis buffer (0.2% Triton X-100 ...
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bioRxiv - Plant Biology 2019Quote: 6x-His purification was conducted in 20 mL gravity columns (VWR) loaded with 4 mL HisPur™ Ni-NTA resin (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... mouse anti-HIS his.h8 monoclonal (1:1000 dilution, VWR #101981-852); goat HRP-anti-rabbit and goat HRP-anti-mouse (1:1000 dilutions ...
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bioRxiv - Bioengineering 2024Quote: HEK293 cells were seeded at 180,000 cells per well in a 24-well tissue culture plate (VWR) containing 500 µl cell culture media 24 hours prior to transfection ...
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bioRxiv - Synthetic Biology 2020Quote: ... in RNase-free 0.2 ml PCR tubes (VWR International) or 1.5 ml DNA LoBind tubes (Eppendorf) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cryosections (10 µm thickness) were collected on His-bond glass slides (VWR) and processed following (Jacobson et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10% fetal bovine serum (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2023Quote: ... 3% SDS (151-21-3, VWR Life Science) and 100 mM Tris–HCl [pH 6.8](1.00317 ...
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bioRxiv - Microbiology 2022Quote: ... together with or without RNase A (cat. no. E866, VWR) treatment ...
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bioRxiv - Microbiology 2022Quote: ... Supernatants were treated with RNase (0.1 µg/ µl; VWR International) for 30 min at 37 °C ...
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bioRxiv - Genetics 2024Quote: ... 1 µl RNase A (VWR Life Science, Cat# E866-5ML), 6.25 µl DNA hybridization buffer (4 g Dextra sulfate sodium salt (Sigma Life Sciences ...
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bioRxiv - Cancer Biology 2021Quote: ... and then diluted using RNase/DNase free water (VWR Life Sciences) until all the concentration of RNA in each sample was 10ng/µL ...
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bioRxiv - Bioengineering 2019Quote: ... 5% w/v RNase free BSA solution (VWR, No. 0332-25G) is poured over the paper ...
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bioRxiv - Molecular Biology 2020Quote: ... One hundred μl TE containing 10 μg RNase A (VWR, A3832) was added to IP and input fractions and the reactions were incubated for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... 3% glucose (VWR), 3.5% active dried yeast (SLS) ...
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bioRxiv - Bioengineering 2022Quote: ... N-isopropylacrylamide (NIPAm) and sucrose (RNASE & DNASE free) was purchased from VWR Life Science (Radnor ...
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bioRxiv - Genomics 2023Quote: ... The pellet was then treated with ammonium acetate and RNase A (VWR). DNA was precipitated with isopropanol and stored in Tris-EDTA buffer at a pH of 8.0 (VWR) ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 mM MgCl2 (VWR), 0.1% NP-40 substitute (Sigma) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... containing 6-His tag and TEV cleaving site.19 In 50 mM HEPES (VWR, PA, USA), pH 7.5 including 50 mM potassium sulfate (Honeywell ...
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bioRxiv - Cell Biology 2019Quote: ... Cell suspensions were incubated with 100 µL 10 mg/mL RNAse A (VWR) for 30 min at 37oC ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant human TNF-alpha (PeproTech/VWR) was used at a concentration of 10 ng/ml.
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bioRxiv - Genomics 2021Quote: All pipette tips used were RNase-free and DNase-free (Cat. #76322 series, VWR). RNA was isolated from livers from n=4 mice from each of four treatment groups ...
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bioRxiv - Cancer Biology 2019Quote: ... G418 (VWR, 970-3-058), for several passages ...
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bioRxiv - Neuroscience 2022Quote: ... 3% Acetonitrile (VWR Cat#83640.320), 1 μg of LysC (Wako Cat#129-02541) ...
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bioRxiv - Neuroscience 2023Quote: ... and 3% hydrogen peroxide (VWR) for 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... and 3 % of BSA (VWR) in PBS ...
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bioRxiv - Bioengineering 2020Quote: ... 1-(3-(Dimethylamino)propyl)-3-ethylcarboiimide hydrochloride (EDC-HCL; 0.5 g, 2.6 mmol, 1.02 equiv., VWR) and NHS-ester (0.65 g ...
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bioRxiv - Cell Biology 2023Quote: ... covered with a glass plate (3" x 3" glass plate from VWR Vertical Gel Electrophoresis Systems), exposed to 7.5 minutes of glass plate filtered (UVA only ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mM MnCl2 (VWR; Cat. # 1.05927.1000), 3 µM Na- orthovanadate (Sigma ...
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bioRxiv - Genomics 2022Quote: ... 3 mM MgCl2 (VWR, 97062-848) supplemented with 0.1% SUPERase•In™ RNase Inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Sf9 insect cells (VWR, #EM71104-3) were maintained in ExCell 420 serum-free media (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL of each rescuer strain was inoculated into 500 μL of SC -his media in 96 well 2 mL deep well plate format (VWR) and shaken at 900 rpm at 30°C ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 3% Bovine Serum Albumin (VWR, #97061-416) in PBS ...
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bioRxiv - Microbiology 2024Quote: ... on 3% agarose gel (VWR, Radnor, PA) with RedSafe Nucleic Acid Staining Solution (Bulldog Bio ...
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bioRxiv - Systems Biology 2024Quote: ... in 3% BSA (VWR Cat# 97061-422) in PBS for 4 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... for 2 hours at 56°C and 50 μg of RNase A (VWR International, Radnor, PA, USA) for 30 min ...