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Citations for VWR :
1 - 50 of 1130 citations for Recombinant Mouse Tissue Inhibitor Of Metalloproteinase 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Biochemistry 2023Quote: ... mouse anti-HIS his.h8 monoclonal (1:1000 dilution, VWR #101981-852); goat HRP-anti-rabbit and goat HRP-anti-mouse (1:1000 dilutions ...
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bioRxiv - Immunology 2023Quote: Mouse tissues were homogenized using soft tissue homogenizing beads (VWR, Radnor, PA). Total RNA was isolated using TRIzol® (Life Technologies ...
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bioRxiv - Physiology 2021Quote: Freshly dissected mouse tissues were rinsed in PBS (VWR, cat. No. 437117K) and then immediately transferred into ice-cold fixative solution of 4% paraformaldehyde (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... recombinant β-lactamase (VWR) was added ...
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bioRxiv - Bioengineering 2023Quote: ... Mouse uterus cross-section tissue was fixed for 30 minutes in 4% formaldehyde (VWR) before blocking for 30 minutes in 10% Normal Goat Serum (50062Z ...
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bioRxiv - Biophysics 2022Quote: ... His-bind resin was purchased from VWR.
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bioRxiv - Neuroscience 2020Quote: Mouse brains were collected through flash freezing in OCT Tissue Tek medium (VWR #25608-930) in dry ice ...
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bioRxiv - Molecular Biology 2020Quote: ... Tissues were stained with DAPI (1:5,000 TCA2412-5MG, VWR) immediately prior to imaging ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant human TNF-alpha (PeproTech/VWR) was used at a concentration of 10 ng/ml.
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bioRxiv - Neuroscience 2024Quote: ... samples were homogenized with a tissue grinder dounce (WHEATON® Dounce Tissue Grinder 1 mL, VWR, Radnor, Pennsylvania, USA) in 150 mM NaCl (S3014 Sigma-Aldrich - Merck ...
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bioRxiv - Molecular Biology 2022Quote: ... and anti-mouse (1:10,000; VWR)-conjugated to horseradish peroxidase HRP.
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bioRxiv - Cancer Biology 2022Quote: ... tumor-bearing mouse brains and flank tumors were homogenized in a pre-chilled glass tissue grinder (VWR) with RIPA lysis buffer containing protease and phosphatase inhibitors (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... 1 mM EDTA pH 8.0) using a glass tissue homogenizer (VWR). Subsequently ...
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bioRxiv - Synthetic Biology 2023Quote: Recombinant murine thrombopoietin (TPO) (VWR, 10770-952).
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bioRxiv - Genomics 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Immunology 2023Quote: ... Tissues were flash frozen in Tissue-Tek Cryomold (VWR) the next day ...
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bioRxiv - Cell Biology 2021Quote: ... Reduction of temperature to 25 °C and induction recombinant gene expression by addition of 1 mM IPTG (VWR) was triggered automatically by pH (7.1) ...
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bioRxiv - Developmental Biology 2020Quote: ... 65.86mL ddH2O) with 1:200 protease inhibitor cocktail (EMD Millipore/VWR catalogue #CA80053-852 ...
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bioRxiv - Cell Biology 2023Quote: ... containing 1% IFS and HALT protease inhibitors (VWR, cat. no. PI78440) was added to the cells containing intracellular parasites before they were scraped and syringe-released ...
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bioRxiv - Biophysics 2023Quote: ... and GAPDH (1:5000, 101983-284 mouse, VWR). Membranes were then washed and incubated with secondary antibodies for 1 hour (both 1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... goat-α-mouse-CF568 (1:500, VWR; AB_10559187), and goat-α-guinea pig-AlexaFluor647 (1.500 ...
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bioRxiv - Neuroscience 2019Quote: ... the tissue was homogenized with a tissue homogenizer (VWR #4320202) in ice-cold radioimmunoprecipitation assay (RIPA ...
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bioRxiv - Immunology 2019Quote: ... CNS tissue were homogenized using a PTFE Tissue Grinder (VWR) and were incubated for 30 minutes at 37°C in homogenization solution (HBSS containing 4 ng mL−1 liberase and 25 ng mL−1 DNase) ...
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bioRxiv - Bioengineering 2023Quote: ... tissues were washed in 1% (wt/vol) sodium dodecyl sulfate (SDS) (VWR, #97062) for 24 hours or until white transparent tissue was obtained ...
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bioRxiv - Bioengineering 2023Quote: ... tissues were washed in 1% (wt/vol) sodium dodecyl sulfate (SDS) (VWR, #97062) for 24 hours or until white transparent tissue was obtained ...
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bioRxiv - Physiology 2024Quote: ... tissues were homogenized in 1 mL of RiboZol (Cat no. N580, VWR, USA), and then total RNA was precipitated in 500 μL of isopropanol ...
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bioRxiv - Plant Biology 2019Quote: 6x-His purification was conducted in 20 mL gravity columns (VWR) loaded with 4 mL HisPur™ Ni-NTA resin (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... by combining compounds with 0.5 μg/mL recombinant luciferase in 1x PBS buffer and 1 mg/mL BSA (VWR). Reactions were initiated by the addition of 30 μL luciferin substrate to wells ...
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bioRxiv - Developmental Biology 2024Quote: ... 100ng/mL recombinant human Noggin (VWR International; 120-10C), 100ng/mL recombinant human FGF10 (Peprotech ...
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bioRxiv - Immunology 2021Quote: ... Tissue was frozen in Tissue-Tek® OCT (VWR; 25608-930), cut on a Leica cryostat ...
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bioRxiv - Microbiology 2021Quote: Lung tissue was homogenised using a VDI 12 tissue homogeniser (VWR) in 1 ml of TRizol reagent (Ambion ...
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bioRxiv - Microbiology 2021Quote: Lung tissue was homogenised using a VDI 12 tissue homogeniser (VWR) in 1 m sterile PBS and filtered through a 70 μm cell strainer (2236348 Fisherbrand ...
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bioRxiv - Cell Biology 2020Quote: ... tissues were mounted in Tissue Tek cryomolds filled with Tissue-Plus Optimal Cutting Temperature (OCT) compound (VWR Scientific Products). Tissue molds were placed on dry ice until the OCT was set ...
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bioRxiv - Microbiology 2021Quote: ... mouse monoclonal anti-GAPDH (VWR 1E6D9; 1:5,000 dilution), rabbit polyclonal anti-ACAT1 (Cell Signaling 44276 ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Colon tissues were homogenized for 1 minute using a disperser (T10 ULTRA-TURRAX; VWR) on ice ...
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bioRxiv - Developmental Biology 2022Quote: ... Cryosections (10 µm thickness) were collected on His-bond glass slides (VWR) and processed following (Jacobson et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10% fetal bovine serum (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (Gibco ...
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bioRxiv - Cancer Biology 2019Quote: ... Tissues were then incubated in a 1:1 mixture of 30% sucrose and Tissue-Tek optimal cutting temperature compound (OCT, VWR) for 30 min ...
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bioRxiv - Developmental Biology 2020Quote: ... The tissues were imbedded in Tissue-Tek OCT compound (VWR, Cat# 25608-930) and stored at -80°C until sectioning ...
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bioRxiv - Cell Biology 2023Quote: Immunohistochemistry was performed on 10 μm fixed tissue sections prepared using a Leica cryostat on tissues frozen in OCT compound (Tissue-Tek, VWR Scientific, 4583). Whole-mount immunohistochemistry was performed as described.49 Mice were perfused with ice cold PBS ...
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bioRxiv - Microbiology 2019Quote: 100 μg of lung tissue was homogenised using a VDI 12 tissue homogeniser (VWR) in 1 mL of TRizol reagent (Ambion ...
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bioRxiv - Molecular Biology 2021Quote: ... rose tissue was macerated in 1 mL of phosphate buffer saline (1X PBS) (VWR, Radnor, PA), 0.05% Tween 20 ...
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bioRxiv - Bioengineering 2019Quote: ... (Tissue Tek, VWR, Bridgeport, NJ). 5 µm thick sections were brought to room temperature and fixed in 10% neutral buffered formalin for 4 min ...
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bioRxiv - Cell Biology 2023Quote: ... and mechanical tissue homogenizer (VWR International Pellet Mixer ...
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bioRxiv - Cancer Biology 2023Quote: ... ∼1X1X0.5 cm3 tissue blocks were embedded in Tissue-Tek OCT compound (VWR, cat. no. 00411243), snap-frozen in isopentane ...
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bioRxiv - Cell Biology 2024Quote: Protein was collected by lysing worms in a 1:1 ratio of worm pellet to lysis buffer using a tissue grinder (VWR 62400-675) kept cold on ice ...