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Citations for VWR :
1 - 50 of 99 citations for Recombinant Human Interleukin 7 Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant human TNF-alpha (PeproTech/VWR) was used at a concentration of 10 ng/ml.
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bioRxiv - Developmental Biology 2024Quote: ... 100ng/mL recombinant human Noggin (VWR International; 120-10C), 100ng/mL recombinant human FGF10 (Peprotech ...
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bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
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bioRxiv - Immunology 2020Quote: ... recombinant β-lactamase (VWR) was added ...
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bioRxiv - Biophysics 2022Quote: ... His-bind resin was purchased from VWR.
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bioRxiv - Immunology 2024Quote: ... the sera were treated with receptor-destroying enzyme (Denka Seiken, VWR), incubated overnight at 37°C ...
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bioRxiv - Synthetic Biology 2023Quote: Recombinant murine thrombopoietin (TPO) (VWR, 10770-952).
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bioRxiv - Cell Biology 2020Quote: ... dechorionated in 7% Sodium Hypochlorite solution (VWR) and washed extensively with MilliQ water ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Plant Biology 2019Quote: 6x-His purification was conducted in 20 mL gravity columns (VWR) loaded with 4 mL HisPur™ Ni-NTA resin (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... mouse anti-HIS his.h8 monoclonal (1:1000 dilution, VWR #101981-852); goat HRP-anti-rabbit and goat HRP-anti-mouse (1:1000 dilutions ...
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bioRxiv - Developmental Biology 2023Quote: ... or xylene (VWR, Randor, VA, 1330-20-7) and rehydrated in a series of graded ethanol washes (100% ...
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bioRxiv - Bioengineering 2024Quote: ... OTMs on day 3 or day 7 post-inoculum (day 10/14 post-seeding) were punched into 7 mm discs using a biopsy punch (VWR, Philadelphia) to fit into a Transwell insert (#29442-082 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cryosections (10 µm thickness) were collected on His-bond glass slides (VWR) and processed following (Jacobson et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10% fetal bovine serum (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (Gibco ...
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bioRxiv - Developmental Biology 2019Quote: ... Pancreas sections (7 μm thick) mounted on polysine slides (VWR) were de-waxed ...
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bioRxiv - Developmental Biology 2022Quote: ... 100ul of deionized Formamide (VWR Catalog # CAS-75-12-7) was added to 900ul of hybridization buffer (Stellaris ...
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bioRxiv - Genetics 2023Quote: The muscarinic receptor antagonist atropine ((RS)-(8-Methyl-8-azabicyclo[3.2.1]oct-3-yl, VWR Cat. TCA0754-005G) was dissolved in ethanol and added to 1M (HS ...
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bioRxiv - Microbiology 2020Quote: ... pH 7) plus 0.3 mg/liter methylene blue (VWR, Radnor, PA) to prevent microbial growth for the first 6 hours ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins were dialyzed in a Slide-a-lyzer (VWR, 514-0172) overnight in ATPase buffer (100mM potassium acetate ...
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bioRxiv - Biophysics 2020Quote: ... Embryos were dechorionated by short immersion in 7% sodium hypochlorite solution (VWR). After extensive rinsing with water ...
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bioRxiv - Cell Biology 2020Quote: ... The embryos or eggs were dechorionated in 7% Sodium Hypochlorite solution (VWR), washed thoroughly in milliQ water ...
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bioRxiv - Biophysics 2023Quote: ... Embryos were dechorionated by short immersion in 7% sodium hypochlorite solution (VWR). After extensive rinsing with water ...
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bioRxiv - Evolutionary Biology 2023Quote: ... containing 6-His tag and TEV cleaving site.19 In 50 mM HEPES (VWR, PA, USA), pH 7.5 including 50 mM potassium sulfate (Honeywell ...
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bioRxiv - Genetics 2021Quote: ... Staining was performed with 10µM H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate, VWR #89138-260) for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... transferred it to a 7 kilodalton cut-off SnakeSkinTM dialysis tubing (PierceTM, VWR), added Ulp1 protease [0.5 μM final concentration] and let it dialyze overnight at 4 °C in buffer A (with 7% glycerol ...
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bioRxiv - Developmental Biology 2022Quote: ... tjTS>lglRNAi ovaries (with no UAS-tagged fluorescence reporter) were dissected in Grace’s Insect Basal medium (VWR; #45000-476), and incubated in Dihydroethidium (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... injections of tamoxifen (100 mg/kg, dissolved in corn oil, VWR, #9001-30-7). The expression of GCaMP6f was verified by immunohistochemistry (with antibodies targeting GFP and neural marker NeuN).
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bioRxiv - Microbiology 2022Quote: ... scintillation vials (7 ml and 20 ml) and Coumarin C6 were purchased from VWR International (Radnor ...
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bioRxiv - Microbiology 2023Quote: ... the wells were overlaid with 2 mL/well of 7% Formaldehyde (VWR,10790-708) for 30 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Molecular Biology 2020Quote: ... and recombinant protein expression was induced by addition of 0.5 mM isopropyl-β-D-thiogalactopyranoside (VWR) for 16–18 h at 25°C ...
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bioRxiv - Biophysics 2021Quote: ... The cells were left for 7 days and then fixed using 100% methanol (MeOH) (VWR, BDH2029) and stained with a crystal violet (Sigma ...
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bioRxiv - Neuroscience 2022Quote: Primary microglia were plated at 7×106 cells/well in 6-well plates (VWR #10062-894) and incubated at 5% CO2 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL of each rescuer strain was inoculated into 500 μL of SC -his media in 96 well 2 mL deep well plate format (VWR) and shaken at 900 rpm at 30°C ...
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bioRxiv - Cell Biology 2021Quote: First trimester placental villous tissue (7-10 weeks of gestation) was snap-frozen in OCT compound (VWR) and 5µm cryosections of tissue were cut and fixed in ice-cold acetone for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Reduction of temperature to 25 °C and induction recombinant gene expression by addition of 1 mM IPTG (VWR) was triggered automatically by pH (7.1) ...
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bioRxiv - Plant Biology 2021Quote: ... 7-day-old seedlings grown on hydroponic media were mounted on coverslips (24×50 mm No. 1½, VWR) using medical adhesive (Stock No ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was homogenized in a 2 ml lysis buffer using a Dounce tissue grinder (7 ml, VWR). After addition of a 1.8 M sucrose solution (4 mL) ...
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bioRxiv - Cell Biology 2022Quote: ... were grown to O.D 0.3-0.6 and incubated with non-fluorescent Halo tag ligand 7-bromo-1-heptanol (7BRO, 100μM, VWR #AAH54762) for 10 min to irreversibly mask all the pre-existing ribosomal proteins ...
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bioRxiv - Cell Biology 2021Quote: Human MSCs were fixed with 4% paraformaldehyde (PFA, VWR International) in deionized (DI ...
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bioRxiv - Biochemistry 2022Quote: ... The human cell lines HEK293T were maintained in DMEM (VWR) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... by combining compounds with 0.5 μg/mL recombinant luciferase in 1x PBS buffer and 1 mg/mL BSA (VWR). Reactions were initiated by the addition of 30 μL luciferin substrate to wells ...
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bioRxiv - Developmental Biology 2021Quote: The differentiated human iPSCs were fixed on glass coverslips (VWR, Denmark) in 4% PFA for 15 min at RT and stored at 4°C until use ...
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bioRxiv - Cell Biology 2024Quote: ... P/S and 2% human serum (VWR/BioWest, cat. #S4190-100). The culture insert’s apical site was supplemented with 300uL and the basolateral site with 800uL media ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then subcultured 1:1000 into 7 mL of MSgg in a 6-well polyvinyl plate (VWR, PA, USA). Plates were incubated in static conditions at 30°C for 3 days and were then imaged using a Leica MZ10F macroscope and Leica DMC2900 camera ...