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Citations for VWR :
1 - 50 of 150 citations for Recombinant Human EGFR protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant human TNF-alpha (PeproTech/VWR) was used at a concentration of 10 ng/ml.
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bioRxiv - Developmental Biology 2024Quote: ... 100ng/mL recombinant human Noggin (VWR International; 120-10C), 100ng/mL recombinant human FGF10 (Peprotech ...
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bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins were dialyzed in a Slide-a-lyzer (VWR, 514-0172) overnight in ATPase buffer (100mM potassium acetate ...
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Molecular Biology 2020Quote: ... and recombinant protein expression was induced by addition of 0.5 mM isopropyl-β-D-thiogalactopyranoside (VWR) for 16–18 h at 25°C ...
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bioRxiv - Microbiology 2023Quote: ... A surface capture lawn was prepared with 50 ug/mL of goat anti-Human IgG Fc secondary antibody (VWR, 103255-066) in 10 mM sodium acetate (pH 4.5)/0.01% Tween ...
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bioRxiv - Immunology 2020Quote: ... recombinant β-lactamase (VWR) was added ...
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bioRxiv - Synthetic Biology 2023Quote: ... APC conjugated (30-F11) (VWR, 103111).
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10 % FCS (VWR International, S181B) and 1 % Pen/Strep (Life Technologies ...
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bioRxiv - Biophysics 2020Quote: ... and APC at pH 7.4 and sonicated (VWR Scientific Branson Sonifier 250 Microtip limit 7 ...
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bioRxiv - Synthetic Biology 2023Quote: Recombinant murine thrombopoietin (TPO) (VWR, 10770-952).
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bioRxiv - Neuroscience 2023Quote: ... supplemented with HEPES and 10% fetal calf serum (FCS) (VWR) 37°C/5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... with 10% fetal calf serum (FCS premium, S. Amer. Orig, VWR), 2 mM L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... Vero E6 (ATCC CRL- 1586) was cultured in DMEM supplemented with 5 % FCS (VWR) and 1 % penicillin−streptomycin−glutamine (PSG ...
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bioRxiv - Genetics 2023Quote: ... Labeled oligos were purified using MicroSpin G-25 Columns (VWR, #95017-621), then scintillation counts were performed using Liquid Scintillation Analyzer Tri-Carb 4910 TR (Perkin Elmer) ...
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bioRxiv - Genomics 2021Quote: ... and 5mL fractions were removed and filtered (70uM) into RPMI supplemented with 2% FCS (VWR) three times ...
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bioRxiv - Developmental Biology 2022Quote: ... tjTS>lglRNAi ovaries (with no UAS-tagged fluorescence reporter) were dissected in Grace’s Insect Basal medium (VWR; #45000-476), and incubated in Dihydroethidium (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... fluorescently labeled EV were purified using sepharose CL-2B (17-0140-01, VWR) size exclusion chromatography (SEC ...
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bioRxiv - Synthetic Biology 2023Quote: ... then plated onto YPM supplemented with 60 µg mL-1 5-fluorocytosine (5-FC) (TCF0321, VWR). Colonies that emerged were then transferred onto a new YPM 5-FC agar plate for clonal isolation ...
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bioRxiv - Cancer Biology 2021Quote: ... The labeled samples were pooled together using a 10-µL glass syringe (VWR, Japan), starting always with the CP samples in each TMT set in order to minimize sample loss during the pooling.20 Samples were pooled into MS-sample vials with glass insert (TPX snap ring vial from Genetec ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
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bioRxiv - Molecular Biology 2022Quote: ... We then transferred the column to a labeled DNA LoBind Eppendorf 1.5 mL tube (VWR # 022431021), added 20 uL nuclease-free H2O ...
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bioRxiv - Cell Biology 2021Quote: ... Reduction of temperature to 25 °C and induction recombinant gene expression by addition of 1 mM IPTG (VWR) was triggered automatically by pH (7.1) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein bands were visualized using Blue BANDitTM protein stain (VWR Life Science, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227), and incubated at 100L°C for 10Lmin under reducing conditions ...
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bioRxiv - Cell Biology 2021Quote: Human MSCs were fixed with 4% paraformaldehyde (PFA, VWR International) in deionized (DI ...
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bioRxiv - Biochemistry 2022Quote: ... The human cell lines HEK293T were maintained in DMEM (VWR) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... by combining compounds with 0.5 μg/mL recombinant luciferase in 1x PBS buffer and 1 mg/mL BSA (VWR). Reactions were initiated by the addition of 30 μL luciferin substrate to wells ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The tube was topped off with 45:45:10 1xMMR:L15:FCS to inactivate the papain and strained threw a 70um sieve (VWR 10199-656). The sieve was washed with 25ml 45:45:10 1xMMR:L15:FCS ...
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bioRxiv - Developmental Biology 2021Quote: The differentiated human iPSCs were fixed on glass coverslips (VWR, Denmark) in 4% PFA for 15 min at RT and stored at 4°C until use ...
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bioRxiv - Cell Biology 2024Quote: ... P/S and 2% human serum (VWR/BioWest, cat. #S4190-100). The culture insert’s apical site was supplemented with 300uL and the basolateral site with 800uL media ...
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bioRxiv - Cell Biology 2020Quote: ... Injection mixture was prepared on ice containing 300 ng Tol2 transposase RNA and 500 ng recombinant plasmid and was brought to 10 µl total volume with 0.1% phenol-red (VWR, 470301-974) in water ...
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bioRxiv - Microbiology 2021Quote: Pooled normal human plasma was obtained from Pacific Hemostasis (VWR #95059-698). Factor X and prothrombin-deficient plasma were obtained from Haematologic Technologies (#FX-ID and #FII-ID) ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 100 μg/mL of 5-florocystosine (5-FC) and grown at 24°C as listed in Supplemental Table S1 (Stork et al. 2008) (VWR, Radnor, PA, USA). A 10 mg/mL stock solution of 5-FC was prepared in distilled water and filtered sterilized ...
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bioRxiv - Microbiology 2020Quote: ... 10 μL of the labeled cell suspension was gently pipetted onto 22 x 22 mm No.1 cover glass (VWR) and sealed onto glass slides with clear acrylic nail polish for confocal imaging or onto Lab-Tek chambered #1 cover glass (Thermo Fischer Scientific)(for widefield fluorescence) ...
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bioRxiv - Biophysics 2023Quote: ... The concentration of total proteins was determined with the BCA Protein Assay Kit (VWR International GmbH, Darmstadt, Germany). According to the total protein amount in each sample ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein was estimated using Bradford reagent (VWR).
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred to BiotraceNT membrane (VWR) and incubated with primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... and peqGOLD Protein-Marker I Unstained (VWR) were used as protein markers ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant protein extracts were recovered by centrifugation and protein concentration in samples was determined using Bradford Reagent (VWR).
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bioRxiv - Neuroscience 2024Quote: Detection with 3,3′-diaminobenzidine (DAB) was performed using horseradish peroxidase labeled secondary antibodies and DAB Quanto chromogen and substrate (VWR, Radnor, PA).
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bioRxiv - Immunology 2022Quote: ... Human colon tissue samples were fixed in 4%(w/v) PFA (VWR International) for 24h at RT ...
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bioRxiv - Microbiology 2020Quote: ... Each fraction was resuspended in HEPES-NaCl buffer and 10 μL of each fraction was examined for protein concentration and lipid content using a Bradford protein assay (VWR) and the lipophilic FM4-64 dye (Molecular Probes) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein concentration was estimated by Bradford reagent (VWR) using bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2021Quote: ... Protein concentration was estimated by Bradford reagent (VWR) using bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2021Quote: ... The protein was incubated overnight with TEV (VWR) at 4°C and Histag and TEV was purified out with Ni-NTA beads (GE) ...
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bioRxiv - Biophysics 2022Quote: ... the purified and concentrated protein was incubated with 1:3 protein to biotin ratio of 1mM NHS-PEG4-Biotin (VWR #PI21362) for 1 hr at room temperature ...