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Citations for VWR :
1 - 50 of 180 citations for Recombinant Human CSF2 Protein non tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant human TNF-alpha (PeproTech/VWR) was used at a concentration of 10 ng/ml.
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bioRxiv - Developmental Biology 2024Quote: ... 100ng/mL recombinant human Noggin (VWR International; 120-10C), 100ng/mL recombinant human FGF10 (Peprotech ...
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bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins were dialyzed in a Slide-a-lyzer (VWR, 514-0172) overnight in ATPase buffer (100mM potassium acetate ...
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bioRxiv - Molecular Biology 2020Quote: ... and recombinant protein expression was induced by addition of 0.5 mM isopropyl-β-D-thiogalactopyranoside (VWR) for 16–18 h at 25°C ...
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bioRxiv - Immunology 2020Quote: ... recombinant β-lactamase (VWR) was added ...
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bioRxiv - Biochemistry 2019Quote: ... 1% non-essential amino acids (VWR) and 1% L-glutamine (VWR) ...
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bioRxiv - Physiology 2021Quote: ... 1% non-essential amino acids (VWR) and 1% L-glutamine (VWR) ...
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bioRxiv - Cell Biology 2020Quote: ... in non-hydrous acetone (VWR, 8.22251) and (ii ...
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bioRxiv - Synthetic Biology 2023Quote: Recombinant murine thrombopoietin (TPO) (VWR, 10770-952).
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bioRxiv - Bioengineering 2021Quote: ... non-pyrogenic cell strainer (VWR; Radnor, PA, USA) and washed with 1X PBS ...
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bioRxiv - Microbiology 2020Quote: ... 1% non-essential amino acids (VWR, Cat # 45000-700), 1% sodium pyruvate (VWR ...
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bioRxiv - Plant Biology 2020Quote: ... sealed with polyester non-sterile transparent film (VWR, USA), and scanned (Perfection V600 Photo ...
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bioRxiv - Immunology 2020Quote: ... in non-tissue culture-treated 10 cm dishes (VWR) at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... and non-treated tissue culture (TC) (VWR, Catalogue # 10861-562) plates were used.
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bioRxiv - Physiology 2020Quote: ... After overnight recover in non-treated plastic petri dishes (VWR) containing RPMI 1640 (Corning cellgro ...
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bioRxiv - Neuroscience 2023Quote: ... non-sequentially onto Superfrost Plus slides (48311703, VWR, Lutterworth, UK) so that sections on the same slide were at least 40 μm apart in the z plane ...
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bioRxiv - Immunology 2020Quote: ... and cultured in non-tissue culture treated dishes (10 cm, VWR) in RPMI medium with L-Glutamine containing 10 % FBS ...
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bioRxiv - Immunology 2020Quote: ... non-fat dry milk and 0.1% Tween 20 (VWR, Radnor, PA). Horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2022Quote: ... Zebrafish embryos and larvae were maintained in a non-CO2 incubator (VWR) at 28°C on the same light-dark cycle as the aquatic facility.
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bioRxiv - Cancer Biology 2023Quote: ... or non-treated 6 well plates (VWR 10861-554. 5 ml/well) and incubated for four days in a 37°C oven ...
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bioRxiv - Cell Biology 2019Quote: Transfected or non-transfected cells were cultured on 10 mm round coverglass (VWR). Prior to fixation ...
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bioRxiv - Biophysics 2022Quote: ... induced with 1 mM B-isopropyl-thiogalactoside-non-animal-origin (IPTG-NAO; VWR USA) in the early-mid log phase ca 1.5 h or OD600 of 0.6 and incubated for another 5 h at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were attached to the non-charged side of Superfrost Plus Micro Slides (VWR).
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bioRxiv - Molecular Biology 2019Quote: ... 5% non-fat milk and 1% BSA filtered through a paper filter (qualitative, 415, VWR). Blots were rinsed 1-2 times with Wash Buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Blocking buffer and primary antibody incubation solution was 5% non-fat dairy milk powder (VWR) or 5% (w/v ...
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bioRxiv - Immunology 2020Quote: ... and 50 ng/mL GM-CSF per 10 cm non-tissue culture treated dish (VWR). At day 3 ...
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bioRxiv - Immunology 2022Quote: ... BV2 cells were cultured in non-treated 10 cm tissue culture plates (VWR, #10062-880) in 5% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... Zebrafish embryos and larvae were maintained in embryo medium in a non-CO2 incubator (VWR) at 28°C on the same light–dark cycle as the aquatic facility ...
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bioRxiv - Developmental Biology 2022Quote: ... tjTS>lglRNAi ovaries (with no UAS-tagged fluorescence reporter) were dissected in Grace’s Insect Basal medium (VWR; #45000-476), and incubated in Dihydroethidium (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... Successively the endogenous peroxidases and non-specific binding sites were blocked using 3% H2O2 (VWR chemicals, 23615.248) and Protein-Block solution (DAKO Agilent technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked with 5% non-fat milk or 5% bovine serum albumin (BSA; VWR #97062-904) in TBS before probing overnight at 4°C with primary antibodies diluted in TBS containing 0.2% Tween-20 (TBS-Tw) ...
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bioRxiv - Genomics 2023Quote: SDS treated cells were resuspended in 400 μl of RT mix (final concentration of 1x RT buffer, 500 mM dNTP, 10 mM Biotinylated RT primers, 7.5% PEG 6000 (VWR, 101443-484), 0.4U/ml SUPERase•In™ RNase Inhibitor ...
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bioRxiv - Bioengineering 2021Quote: ... Discs prepared from the non-woven fabrics treated with only the absolute ethanol (≥99.8%, VWR chemicals, AnalaR Normapur) solvent under magnetic stirring for 30 minutes at 24±1°C (Control S ...
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bioRxiv - Microbiology 2020Quote: ... and 6 ml were transferred to 6-well plates (Corning Costar®, non-treated, 392-0213, VWR, Germany). Cultures were incubated for 48 h at 30°C and 40 μmol photons m−2 s−1 of white light at 95 rpm on an orbital shaker ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were grown in the dark overnight in LB in non-treated 96- well plate (VWR, Radnor, PA) at 37°C with 110 rpm shaking ...
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bioRxiv - Cell Biology 2022Quote: ... were grown to O.D 0.3-0.6 and incubated with non-fluorescent Halo tag ligand 7-bromo-1-heptanol (7BRO, 100μM, VWR #AAH54762) for 10 min to irreversibly mask all the pre-existing ribosomal proteins ...
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bioRxiv - Microbiology 2022Quote: ... cells from a rocking culture of swimmers were added to 60 mm non-treated tissue culture plates (VWR) in 2 mL of BHI ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were probed with antibodies diluted in 5% non-fat dry milk in Tris buffered saline (VWR #J640-4L) and 0.1% Tween20 (Fisher Scientific #BP337 ...
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bioRxiv - Cell Biology 2021Quote: ... Reduction of temperature to 25 °C and induction recombinant gene expression by addition of 1 mM IPTG (VWR) was triggered automatically by pH (7.1) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein bands were visualized using Blue BANDitTM protein stain (VWR Life Science, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227), and incubated at 100L°C for 10Lmin under reducing conditions ...
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bioRxiv - Biochemistry 2019Quote: ... were used to inoculate 500 μL starter cultures in non-inducing media (43) with antibiotics in a 96-deep well culture plate (VWR). The 96-well starter cultures were grown 16-20 h at 37 °C with vigorous shaking at 325 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... The integrated vector backbone was removed by growth in non-selective YT media (5 g/l yeast extract (VWR, EM1.03753.0500), 5 g/l tryptone (Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: Wild-type (WT) and TLR4 knockout (KO) BMDMs were thawed and cultured for 3 days in non-Stissue culture treated dishes (VWR) in RPMI 1640 medium with L-Glutamine containing 10 % FBS and 10 % CM (= complete medium) ...
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bioRxiv - Immunology 2021Quote: ... All of the antimicrobials dissolved in non-acidified autoclaved reverse osmosis drinking water except Amphotericin B (VWR Life Science, CA) which was solubilized in DMSO (VWR Life Science ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant Jag1-Fc was immobilized by overnight incubation at 4°C in individual wells of non-tissue culture-treated 6 or 12 well plates (VWR) at a final concentration of 2 µg/mL in DPBS containing 10 µg/mL poly-D-lysine (ThermoFisher Scientific) ...