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Citations for VWR :
1 - 50 of 149 citations for Recombinant Enhanced Green Fluorescent Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Bioengineering 2023Quote: ... stably expressing enhanced green fluorescent protein (eGFP) were generated as described before[45] and cultured on 0.1% gelatin (VWR) pre-coated surfaces in MCDB-131 medium (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins were dialyzed in a Slide-a-lyzer (VWR, 514-0172) overnight in ATPase buffer (100mM potassium acetate ...
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bioRxiv - Molecular Biology 2020Quote: ... and recombinant protein expression was induced by addition of 0.5 mM isopropyl-β-D-thiogalactopyranoside (VWR) for 16–18 h at 25°C ...
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bioRxiv - Immunology 2020Quote: ... recombinant β-lactamase (VWR) was added ...
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bioRxiv - Biophysics 2022Quote: ... His-bind resin was purchased from VWR.
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bioRxiv - Cell Biology 2023Quote: ... After incubation with enhanced chemiluminescence (ECL) substrate (VWR, cat. no. PI32209), autoradiography film (MTC Bio ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant human TNF-alpha (PeproTech/VWR) was used at a concentration of 10 ng/ml.
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bioRxiv - Synthetic Biology 2023Quote: Recombinant murine thrombopoietin (TPO) (VWR, 10770-952).
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bioRxiv - Genomics 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Plant Biology 2019Quote: 6x-His purification was conducted in 20 mL gravity columns (VWR) loaded with 4 mL HisPur™ Ni-NTA resin (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... mouse anti-HIS his.h8 monoclonal (1:1000 dilution, VWR #101981-852); goat HRP-anti-rabbit and goat HRP-anti-mouse (1:1000 dilutions ...
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bioRxiv - Developmental Biology 2024Quote: ... 100ng/mL recombinant human Noggin (VWR International; 120-10C), 100ng/mL recombinant human FGF10 (Peprotech ...
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bioRxiv - Biochemistry 2021Quote: ... PKCδ was visualized using the Amersham Enhanced Chemiluminescence Western blotting detection system (VWR, Mississauga, ON, Canada). Band intensity was quantified using Image J software ...
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bioRxiv - Developmental Biology 2022Quote: ... Cryosections (10 µm thickness) were collected on His-bond glass slides (VWR) and processed following (Jacobson et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10% fetal bovine serum (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... Calcofluor white (Fluorescent brightener 28; #ICNA0215806705) was obtained from VWR International (Radnor ...
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bioRxiv - Bioengineering 2023Quote: ... Fast green and sodium thiosulphate (VWR); Lubrithal (Dechra ...
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bioRxiv - Bioengineering 2023Quote: ... fast green and sodium thiosulphate (VWR); Lubrithal (Dechra ...
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bioRxiv - Bioengineering 2023Quote: ... Fast green and sodium thiosulphate (VWR); Lubrithal (Dechra ...
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bioRxiv - Bioengineering 2024Quote: ... fast green and sodium thiosulphate (VWR); Isoflurane (Dechra ...
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bioRxiv - Immunology 2019Quote: ... PerfeCTa® SYBR® Green FastMix (VWR) was added to cDNA for fluorescence quantification using a Stratagene Mx3000P qPCR System (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2022Quote: ... for which PerfeCTa SYBR Green Fastmix (VWR) was used ...
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bioRxiv - Neuroscience 2022Quote: ... incubated for 5 minutes with fluorescent nuclear DAPI stain (VWR; 1:5000), and mounted with DAKO immunofluorescence mounting media (Life Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... incubated for 5 minutes with fluorescent nuclear DAPI stain (VWR; 1:10000), and mounted with Slow fade gold antifade reagent (Life Technologies) ...
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bioRxiv - Molecular Biology 2022Quote: ... Following fluorescent imaging the gel was stained with 1% Coomassie (VWR 95043-420) for 20 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... using PerfeCTa SYBR green (VWR, Radnor, PA, USA). Reactions were performed on a 384 well plate ...
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bioRxiv - Evolutionary Biology 2023Quote: ... containing 6-His tag and TEV cleaving site.19 In 50 mM HEPES (VWR, PA, USA), pH 7.5 including 50 mM potassium sulfate (Honeywell ...
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bioRxiv - Bioengineering 2024Quote: ... AB_11125142) or an anti-mouse secondary antibody (1:3000, Cat# 170-6516, RRID: AB_11125547) and enhanced chemiluminescence reagent (VWR, Cat # 490005-018). The signal was visualized using a C-DiGit Blot Scanner (Li-Cor) ...
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bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
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bioRxiv - Developmental Biology 2022Quote: ... tjTS>lglRNAi ovaries (with no UAS-tagged fluorescence reporter) were dissected in Grace’s Insect Basal medium (VWR; #45000-476), and incubated in Dihydroethidium (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... All sections were counter stained with Fast Green (VWR) and imaged (Keyence BZ-X810 microscope).
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bioRxiv - Microbiology 2022Quote: ... 2 µL 5x SYBR green (VWR, CN 12001-796), and 1.4 µL nuclease-free water ...
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bioRxiv - Biochemistry 2023Quote: Nucspot green fluorescence nuclear stain was purchased from VWR and used per manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... using PerfeCTa SYBER Green fast mix (VWR #101414-270). Expression of the AMPs Drosocin ...
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bioRxiv - Biochemistry 2019Quote: ... with Perfecta SYBR Green Master Fast Mix (VWR, 101414-286)38 ...
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bioRxiv - Neuroscience 2019Quote: ... PerfeCTa SYBR Green FastMix Quanta (VWR Cat No. 101414-150) was used (Table S12 for PCR Primers).
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed using SYBER-green (VWR: Cat #101414-144) on a BioRad iQ5 Thermocycler ...
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL of each rescuer strain was inoculated into 500 μL of SC -his media in 96 well 2 mL deep well plate format (VWR) and shaken at 900 rpm at 30°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
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bioRxiv - Cell Biology 2020Quote: ... The Brilliant II SYBR Green QRT-PCR 1-Step Master Mix (VWR) was used to convert RNA to cDNA and amplify specific RNA regions on the CFX96 Touch real-time PCR detection system (Bio-Rad) ...
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bioRxiv - Physiology 2022Quote: ... qRT-PCR analysis was performed using SYBR Green qPCR Master Mix (VWR). Primers were designed for gene specificity and to cross exon-exon junctions using Primer-BLAST (www.ncbi.nlm.nih.gov/tools/primer-blast/ ...
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bioRxiv - Microbiology 2024Quote: ... 12.5 µL of EconoTaq PLUS green 2X Master Mix (VWR, Radnor, PA), 1 µL of 10 µM 16S 8F primer ...
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bioRxiv - Cell Biology 2021Quote: ... Reduction of temperature to 25 °C and induction recombinant gene expression by addition of 1 mM IPTG (VWR) was triggered automatically by pH (7.1) ...
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bioRxiv - Cell Biology 2022Quote: ... were grown to O.D 0.3-0.6 and incubated with non-fluorescent Halo tag ligand 7-bromo-1-heptanol (7BRO, 100μM, VWR #AAH54762) for 10 min to irreversibly mask all the pre-existing ribosomal proteins ...
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bioRxiv - Biochemistry 2022Quote: ... Protein bands were visualized using Blue BANDitTM protein stain (VWR Life Science, Darmstadt, Germany).