Labshake search
Citations for VWR :
1 - 50 of 131 citations for Recombinant E. coli MUG Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins were dialyzed in a Slide-a-lyzer (VWR, 514-0172) overnight in ATPase buffer (100mM potassium acetate ...
-
bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
-
bioRxiv - Immunology 2022Quote: ... gentamycin (10 μg/ml for plasmid maintenance in E. coli; 100 μg/ml for plasmid maintenance in P. aeruginosa, VWR, Cat. No. 97062-974).
-
bioRxiv - Molecular Biology 2020Quote: ... and recombinant protein expression was induced by addition of 0.5 mM isopropyl-β-D-thiogalactopyranoside (VWR) for 16–18 h at 25°C ...
-
bioRxiv - Immunology 2020Quote: ... recombinant β-lactamase (VWR) was added ...
-
bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... Recombinant human TNF-alpha (PeproTech/VWR) was used at a concentration of 10 ng/ml.
-
bioRxiv - Developmental Biology 2022Quote: ... H&E routine stain was performed using Leica ST5010 Autostainer XL and Leica ST Infinity H&E Staining System kit (VWR#3801698) according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2023Quote: Recombinant murine thrombopoietin (TPO) (VWR, 10770-952).
-
bioRxiv - Biochemistry 2023Quote: ... coli BL21(DE3) (Stratagene Europe/VWR, Darmstadt, Germany). Protein expression was induced with 0.5 mM isopropyl β-d-thiogalactopyranoside for 18 h at 20 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... and digested with 1 mg/mL pronase E (39052, VWR) per 1 g tissue for 6-8 h at 37 °C and 5% CO2 under constant agitation ...
-
bioRxiv - Microbiology 2023Quote: ... coli strains were grown on Luria–Bertani agar (VWR) or in Luria–Bertani broth (VWR ...
-
bioRxiv - Developmental Biology 2024Quote: ... 100ng/mL recombinant human Noggin (VWR International; 120-10C), 100ng/mL recombinant human FGF10 (Peprotech ...
-
bioRxiv - Microbiology 2021Quote: ... coli DH5α was grown in Luria Bertani (LB) broth (VWR), and E ...
-
bioRxiv - Bioengineering 2019Quote: ... coli loaded microbeads were cultures in Lysogeny broth (LB; VWR). For the generation of degradable alginate microbeads ...
-
bioRxiv - Microbiology 2021Quote: ... coli Rossetta2 (DE3) was grown in Terrific Broth (TB, VWR) for protein purification ...
-
bioRxiv - Microbiology 2019Quote: ... coli BL21 (DE3) was grown in Terrific broth (VWR, PA, USA) for protein purification ...
-
bioRxiv - Microbiology 2024Quote: ... Escherichia coli DH5α strains were grown in Luria-Bertani Broth (VWR) at 37°C and supplemented with 200 μg/mL hygromycin and 50 μg/mL kanamycin ...
-
bioRxiv - Genomics 2024Quote: ... 5 mM Na3 citrate 2H2O) with ∼75u/mL Pronase E (VWR, Radnor, PA) was added to each sample ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Tygon tubing was replaced by flexible pumping tubing (Masterflex Tygon E-3603, VWR). Outflow tubing were connected to outlet ports ...
-
bioRxiv - Bioengineering 2020Quote: ... coli strains were cultured in lysogeny broth (LB) (VWR International, Randor, PA) at 37 °C with rotary shaking at 250 rpm ...
-
bioRxiv - Microbiology 2019Quote: ... coli DH5α was grown in Luria Bertani (LB) broth (VWR, PA, USA), and E ...
-
bioRxiv - Microbiology 2021Quote: ... coli BL21 Star (DE3) or OverExpress C43 (DE3) (Lucigen/VWR, Radnor, PA). Following induction with IPTG ...
-
bioRxiv - Microbiology 2022Quote: ... coli DH5α was propagated in Luria Bertani (LB) broth (VWR, PA, USA), and E ...
-
bioRxiv - Microbiology 2023Quote: ... coli were grown overnight to stationary phase in LB Lenox Broth (VWR) before cells were washed 2x with PBS and OD600 determined by NanoDrop 2000c (Thermo Scientific) ...
-
bioRxiv - Cell Biology 2022Quote: ... coli DB (DBS0350636) in a type 25384-302 petri dish (VWR, Radnor, PA). 100 μg/ml dihydrostreptomycin and 100 μg/ml ampicillin were used to kill E ...
-
bioRxiv - Microbiology 2022Quote: ... coli BL21 (DE3) was propagated in Terrific broth (TB) medium (VWR, PA, USA) for protein purification.
-
bioRxiv - Cell Biology 2023Quote: ... coli DB (DBS0350636) in a type 2538-4302 petri dish (VWR, Radnor, PA). 100 μg/ml dihydrostreptomycin and 100 μg/ml ampicillin were used to kill E ...
-
bioRxiv - Biophysics 2019Quote: ... The vial with the cell suspension was connected via Tygon S3 E-3603 tubing (VWR, inner diameter 1/32" ...
-
bioRxiv - Bioengineering 2020Quote: All Escherichia coli strains were cultured in lysogeny broth (LB) (VWR International, Randor, PA) at 37 °C with rotary shaking at 250 rpm ...
-
bioRxiv - Microbiology 2023Quote: ... coli E1 strain was grown overnight to stationary phase in LB Lenox Broth (VWR) before cells were washed 2x with PBS and OD600 determined by NanoDrop 2000c (Thermo Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... coli expression constructs were grown overnight shaking at 37 °C in Terrific Broth (VWR) supplemented with 5 mM MgSO4 and kanamycin (50μg/ml ...
-
bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
-
bioRxiv - Microbiology 2023Quote: ... then treated with Pronase E (100µg/ml in Tris-HCl (pH 7.2) + 0.06% NaCl) (VWR Chemicals) for 2 hours at 60C to remove proteins covalently bound to PG ...
-
bioRxiv - Plant Biology 2023Quote: ... coli DH5α and positive clones were selected via Colony PCR using Taq polymerase (VWR, Radnor, USA). Correct insertion was checked via Sanger sequencing at Eurofins Genomics (Ebersberg ...
-
bioRxiv - Microbiology 2023Quote: ... coli cells were grown at 37 °C with aeration in either LB (Luria-Bertani broth; VWR) or LA (Luria Agar ...
-
bioRxiv - Cell Biology 2023Quote: ... coli Gold bacteria were cultured in Luria-Bertani (LB) medium (Broth, Miller, VWR, Radnor, PA, USA) supplemented with 150 μg/ml ampicillin for 4.5 and 6 hours ...
-
bioRxiv - Biochemistry 2022Quote: ... and were treated for 2hrs at 60°C with activated Pronase E (100µg/ml final concentration, pre-treated for 2hrs at 60°C in the digestion buffer; supplier: VWR). The sacculi were incubated at 100°C for 10min to inactivate the Pronase E and were washed 3 times with 1ml of MQ water ...
-
bioRxiv - Biochemistry 2022Quote: ... and were treated for 2hrs at 60°C with Pronase E (100µg/ml final concentration, pre-treated for 2hrs at 60C in the digestion buffer; supplier: VWR). The sacculi were incubated at 100°C for 10min to inactivate the Pronase E ...
-
bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
-
bioRxiv - Microbiology 2023Quote: ... coli (E23) CRISPRi cell lines (details below) were grown overnight to stationary phase in LB Lenox Broth (VWR) before dilution 1:50 into fresh media in the morning ± 2 µM anhydrotetracycline ...
-
bioRxiv - Biophysics 2021Quote: ... The sandwich consists of a bottom layer of PAA gel (E ≈ 1800 Pa) and a top layer of a 12 mm round glass cover slip (VWR).
-
bioRxiv - Cell Biology 2021Quote: ... Reduction of temperature to 25 °C and induction recombinant gene expression by addition of 1 mM IPTG (VWR) was triggered automatically by pH (7.1) ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein bands were visualized using Blue BANDitTM protein stain (VWR Life Science, Darmstadt, Germany).
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227), and incubated at 100L°C for 10Lmin under reducing conditions ...
-
bioRxiv - Developmental Biology 2023Quote: ... transgenic Hydra from strains Tg(actin1:GFP)rs3-in and Tg(tba1c:mNeonGreen)cj1-gt were dissociated using Pronase E (VWR, E629-1G) as previously described (Siebert et al. ...
-
bioRxiv - Physiology 2023Quote: ... 8 μl of the final polyacrylamide mix were pipetted on 35 mm glass bottom dishes (Cell E&G LLC VWR, USA) which were pretreated with 0.1 M NaOH ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... by combining compounds with 0.5 μg/mL recombinant luciferase in 1x PBS buffer and 1 mg/mL BSA (VWR). Reactions were initiated by the addition of 30 μL luciferin substrate to wells ...
-
bioRxiv - Physiology 2020Quote: ... 5-μm sections were stained with hematoxylin and eosin (H&E) using the Leica Autostainer and mounted in DePeX (VWR, Lutterworth, UK). Adipocyte diameter and pancreatic β-cell islet number and size were quantified using ImageJ software as previously described (7 ...
-
bioRxiv - Cell Biology 2020Quote: ... Injection mixture was prepared on ice containing 300 ng Tol2 transposase RNA and 500 ng recombinant plasmid and was brought to 10 µl total volume with 0.1% phenol-red (VWR, 470301-974) in water ...