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Citations for VWR :
1 - 50 of 83 citations for Real Time Thermal Cycler Thermocycler Accessories since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... the exons of interest in PD-L1 gene were amplified by using specific primers (Table 5) with a PCR thermal cycler (VWR Thermocycler, Leicestershire, England). PCR procedures were performed with a standard reaction mixture (4 μl/sample of Phusion GC Buffer (Thermo Fischer Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The amplification was conducted in Thermal cycler UNO96 (VWR International, Pennsylvania, USA) according to the following program ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription was performed on a VWR Ristretto thermal cycler (VWR International GmbH. Germany). The cDNA concentrations were measured using a Qubit ssDNA assay kit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... The aliquots were individually heated at different temperatures (Doppio 2×48 well Thermal cycler, VWR). After the heat treatment ...
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bioRxiv - Physiology 2021Quote: ... incubated at 37°C for 120 min and inactivated at 85°C for 5 min in a thermal cycler (VWR UNO 96). cDNA was then stored at −20°C until further use ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative real-time PCR was performed using the PerfecCTa SYBR Green FastMix (VWR, catalog # 101414-280) on the Step-One Plus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... Virus titers were quantified and titrated by real-time PCR (QuantaBio ToughMix®, VWR International, Radner, PA) with a TCID50/ml equivalent as previously described (34) ...
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bioRxiv - Cancer Biology 2023Quote: ... Expression of genes of interest was quantified by real-time PCR using SYBR Green method (VWR QUNT95072). Primers used are detailed in supplementary methods ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were amplified with a peqSTAR thermocycler (VWR, Radnor, PA, USA) and the following conditions ...
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bioRxiv - Neuroscience 2024Quote: ... and cooling to 4°C using a thermocycler (Avantor VWR, Radnor, PA, USA). All primers were originally designed using NCBI primer blast software ...
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bioRxiv - Pathology 2021Quote: Viral RNA was quantitated using single-step RT-quantitative real-time PCR (Quanta qScript One-Step RT-qPCR Kit, QuantaBio, Beverly, MA; VWR; #76047-082) with primers and TaqMan® probes targeting the SARS-CoV-2 E gene as previously described (62) ...
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bioRxiv - Biophysics 2020Quote: ... then incubated overnight at 37°C with gentle agitation (VWR Thermal Shake Touch, 900 rpm). Digested peptides extracted into supernatant and transferred to VWR non-stick microcentrifuge tubes ...
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bioRxiv - Biophysics 2020Quote: ... 50% 50mM Am-BIC buffer (pH 7.4) and shaken for 45 minutes at 900 rpm (VWR Thermal Shake Touch). 750 µL of ACN was added to each tube for 30 minutes to dehydrate the gel slices ...
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bioRxiv - Physiology 2023Quote: ... The urothelium was minced with sharp scissors and placed on a thermomixer (VWR Thermal Shake Touch, 89232-908, VWR) at 700 RPM for 2 hours at 37°C ...
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bioRxiv - Physiology 2023Quote: ... The urothelium was minced with sharp scissors and placed on a thermomixer (VWR Thermal Shake Touch, 89232-908, VWR) at 700 RPM for 2 hours at 37°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 mL 5% PVA (w/v) in 200 mM sucrose was incubated for 1 h at 90°C in a thermal shaker lite (VWR international GmbH ...
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bioRxiv - Molecular Biology 2022Quote: ... Homogenates were sonicated three times for 30s each time at room temperature in a water bath sonicator (VWR, 97043-960). Samples were rested on ice for 30s between each cycle ...
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bioRxiv - Immunology 2022Quote: ... 1 time with 1ml of 0.1M Na2CO3 (VWR), 1 time with 1ml of freshly prepared 2M Urea (VWR ...
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bioRxiv - Molecular Biology 2023Quote: ... and 4 times with isopropanol (VWR,9084-03) and 2 times with distilled water for 3 minutes (min ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... The mixture was then incubated at 4°C for 30 minutes with agitation at 1500 rpm using a thermomixer (VWR, Thermal Shake lite).
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bioRxiv - Biophysics 2020Quote: Gel plugs were washed with 50:50 methanol: 50 mM ammonium bicarbonate twice for 40 min with gentle agitation (VWR Thermal Shake Touch, 900 rpm). Gel plugs were dehydrated by adding 500 μL acetonitrile ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed three times 1X PBS (VWR, life science) and resuspended in 1 ml 1X PBS ...
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bioRxiv - Immunology 2022Quote: ... 1 time with 1ml of freshly prepared 2M Urea (VWR) resuspended in 10mM Tris-HCl pH 8.0 (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were washed 2 times with Histochoice Clearing Agent (VWR, H103) and 4 times with isopropanol (VWR,9084-03 ...
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bioRxiv - Bioengineering 2021Quote: ... washed three times with PBS with 0.1% Tween 20 (VWR International AB) (PBST) ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were dounced 20 times in a 7ml stainless tissue grinder (VWR) and then spun at 200g for 5min to pellet large debris ...
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bioRxiv - Developmental Biology 2022Quote: ... Stained slides were washed 3 times in PBS and No.1 coverslips (VWR) were added using ProLong Gold Antifade Mountant (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... then disrupted 5 times with a dounce homogenizer (VWR cat. no. 62400-595). Following mechanical disruption ...
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bioRxiv - Biochemistry 2023Quote: ... Beads were washed 4 times with 0.1% NP-40 substitute (VWR, 97064-730) in tris-buffered saline (TBS) ...
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bioRxiv - Biochemistry 2024Quote: ... The plate was washed three times and TMB (VWR International, 50-76-03) substrate was added ...
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bioRxiv - Neuroscience 2020Quote: ... three times at RT and then incubated with Thiazine-Red (0.05%, cat#27419.123, VWR) for 20 min ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed three times and fixated using 1% formaldehyde (Avantor, VWR, Denmark). Fluorescent intensity was measured with a NovoCyte 3000 Analyzer equipped with three lasers (405 ...
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bioRxiv - Immunology 2023Quote: ... All samples were diluted ten times in low EDTA TE-buffer (VWR, cat.: APLIA8569.0500) in a total volume of 20 μL ...
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bioRxiv - Cell Biology 2020Quote: ... the coverslips were washed three times with PBS and mounted on microscope slides (VWR International) in DAPI-containing Prolong Gold (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... The perineal region was cleaned three times with ethanol and a betadine scrub (VWR, AJ159778), and sterile dissecting shears were used to make a 4–5 mm incision in this region ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed three times in PBS and mounted on microscope slides (VWR International) in Prolong Gold (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: ... 4 mL per time point) in 6-well polystyrene well plates (Greiner Bio-One; VWR). After 4 and 8 hours ...
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bioRxiv - Bioengineering 2023Quote: ... Cell monolayers were then rinsed three times with PBS and fixed with 4% paraformaldehyde (VWR) for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were afterwards washed several times in quencher solution [10 mM sodium ascorbate (VWR), 10 mM sodium azide (VWR) ...
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bioRxiv - Molecular Biology 2021Quote: 2 x 107 BCBL-1 cells per time point were crosslinked with 2% formaldehyde (VWR M134) in prewarmed 1X phosphate buffered saline (PBS ...
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bioRxiv - Plant Biology 2021Quote: Cultures harvested at select time points were fixed with 0.2% glutaraldehyde (VWR, Cat No. 76177-346). Cells were imaged with a Leica DMI6000 B microscope and a 63x (NA1.4 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Stained coverslips were then washed three times with 1x PBS and mounted on microscope slides (VWR) with homemade MOWIOL mounting medium ...
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bioRxiv - Immunology 2023Quote: ... The cells were fixed by dipping 10 times in pre-cooled ice-cold acetone (VWR, #20066.296) in a glass tray ...
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bioRxiv - Cancer Biology 2022Quote: ... beads are washed three times with 1X sodium chloride-sodium phosphate-EDTA buffer (SSPE, VWR, #VWRV0810-4L), added to the purified PCR product ...
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bioRxiv - Neuroscience 2020Quote: ... then washed three times with 0.1 M PB before being mounted onto Superfrost slides (VWR, Radnor, PA) with 0.1% gelatin and coverslipped with Prolong Gold antifade reagent (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... Larvae were then rinsed with PBS 1x and washed 5 times with PBS-triton 0.8% (28817295, VWR) for 10 min with slight agitation ...
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bioRxiv - Bioengineering 2022Quote: ... Devices were washed three more times with PBS and blocked in PBS 5% bovine serum albumin (BSA, VWR) overnight at 4 °C ...
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bioRxiv - Biophysics 2019Quote: ... four times BRB40 buffer (40 mM Pipes [Sigma], pH 6.84, with KOH [VWR], 1mM EGTA [Sigma], 4mM MgCl2 [VWR]) to remove excess F-127 in solution and exchange buffers ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell supernatants were collected at different time points and centrifuged 30min at 10,000 × g at 4°C to eliminate cell debris (centrifuge CT15RE, VWR), then at 100,000 × g for 3h to eliminate larger vesicles (L7-55 Ultracentrifuge ...
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bioRxiv - Immunology 2022Quote: ... The protein fraction was isolated by combining four times the volume of ice cold acetone (VWR Chemicals, PA, USA) to one volume of reconstituted PBMCsec ...