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Citations for VWR :
1 - 41 of 41 citations for Rat TRPV1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were transfected with hTRPC6 (cloned into pcDNA3) or rat TRPV1 using jetPRIME ® transfection reagent (VWR, Lutterworth, UK). Cells were assayed 48 hours after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... cells expressing the various shRNAs were selected with 0.2 mg/ml hygromycin (cat#K547-20ml, VWR) at 72-hours post transfection and maintained at 0.8 mg/ml of hygromycin after cell line purification.
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bioRxiv - Cancer Biology 2020Quote: ... high concentration rat-tail collagen I (VWR) was neutralized with a 1:1 ratio of 100 mM HEPES (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Plasmid extraction (Omega, VWR), endonuclease digestion (New England Biolabs) ...
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bioRxiv - Bioengineering 2023Quote: Collagen I was isolated from frozen rat tails (VWR RLRBT297) based on a previously established method26 ...
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bioRxiv - Bioengineering 2021Quote: ... Microchannels were coated with a rat tail collagen type I (VWR) solution of 0.1 mg/ml in DPBS (rat tail collagen I ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid DNA was amplified and purified using EZNA endo-free plasmid maxi kit (VWR International).
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bioRxiv - Pharmacology and Toxicology 2019Quote: Rats were gently wrapped in an absorbent bench underpad (VWR, Radnor, PA) such that their tails hung freely ...
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bioRxiv - Bioengineering 2021Quote: ... Vascular compartments were coated with a rat tail collagen type I (VWR) solution of 0.1 mg/ml in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Plant Biology 2019Quote: ... Colonies were evaluated by colony PCR followed by plasmid isolation from overnight culture using peqGOLD Plasmid Miniprep Kit I (VWR). All sequences were confirmed by Sanger sequencing (Sequencing Core Facility ...
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bioRxiv - Genetics 2022Quote: ... or hygromycin (FIREWach plasmids, 200 ng/μl, VWR) starting two days after transduction ...
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bioRxiv - Biochemistry 2024Quote: ... peqGOLD Plasmid Miniprep Kit I (VWR Life Science). Successful mutagenesis was verified by Sanger sequencing.
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bioRxiv - Neuroscience 2021Quote: ... The rats were first anesthetized with 400 mg/kg chloral hydrate (VWR International; Poole, England) injected intraperitoneally ...
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bioRxiv - Immunology 2022Quote: ... gentamycin (10 μg/ml for plasmid maintenance in E. coli; 100 μg/ml for plasmid maintenance in P. aeruginosa, VWR, Cat. No. 97062-974).
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bioRxiv - Neuroscience 2022Quote: ... single-housed rats were given 24h concurrent access to one bottle of 20% (v/v) alcohol (VWR International S.A.S. ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: All rats were trained to press the active lever for 45-mg banana-flavoured sucrose pellets (product # 76285-260, VWR) during daily ...
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bioRxiv - Genetics 2020Quote: ... pelleted and purified by the Plasmid Miniprep Kit I (peqGOLD, VWR, Radnor, USA). Inserts of the isolated plasmids were verified by sequencing ...
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bioRxiv - Cell Biology 2022Quote: Plasmids were transfected using jetPRIME® transfection reagent (VWR Scientific Cat # 89129-920) or Lipofectamine™ 2000 Transfection Reagent (Thermo Fisher Scientific #11668027 ...
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bioRxiv - Microbiology 2021Quote: ... histolytica expression plasmid pEhEx backbone (38) using the Gibson Assembly Ultra Kit (VWR). Primers used for Gibson cloning are described in Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were maintained in liquid culture with the appropriate antibiotics (VWR, Radnor, PA). List of plasmids are in Table S1.
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bioRxiv - Systems Biology 2022Quote: Yeast with plasmids were grown in a 96-well deep well plate (VWR) containing 100μl of SC-Ura medium with 2% glucose and grown for ~12 hours at 30°C in a shaking incubator set at 250 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... along with VSV-G and pUVMC packaging plasmids via Jetpei (VWR 89129-916).
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bioRxiv - Neuroscience 2022Quote: Fresh fecal pellets were obtained aseptically by direct defecation of rats into a sterile 120-ml container (VWR, Radnor, PA, USA). Samples were collected from the 153 rats at PD 60 ...
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bioRxiv - Physiology 2023Quote: ... after overnight fasting followed by administration of a saline solution of glucose (2 g/kg or 1.5g/kg, for rats or mice, respectively; VWR Chemicals, Leuven, Belgium), by gavage ...
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bioRxiv - Cancer Biology 2023Quote: ... Appropriate number of acinar cells were then resuspended in premade collagen solution (1 mg/ml rat tail type1 collagen (VWR, Cat. No. CACB354236), 10× Waymouth’s media (Cedarlane Labs ...
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bioRxiv - Physiology 2022Quote: ... the isolation was performed according to the Plasmid Miniprep Kit I (peqGOLD, VWR, Radnor, USA) and verified through sequencing.
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bioRxiv - Genetics 2020Quote: HCT116 cells were transfected with px459sgCTNNB1 and the two donor template encoding plasmids KOZAK_FLAG_mClover3 and KOZAK_V5_mCherry using TransIT (731-0029; VWR) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... we transfect the cells with either WT or mutant SOD1 pCI-Neo plasmid using JetOptimus transfection reagent (VWR) according to the manufacturer’s instructions (31) ...
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Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfectionbioRxiv - Microbiology 2020Quote: ... Equal amounts of plasmid (2 μg total) were co-transfected with 3 μL 25kDa PEI (VWR, Radnor, PA) into 293T cells into a 24-well plate.
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
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bioRxiv - Microbiology 2022Quote: ... Each transformation consisted of 40μL of competent cells and 10ng of the pFAB5516 plasmid library transferred to a chilled cuvette (1mm gap, VWR). Cuvettes were electroporated using a BTX™-Harvard Apparatus ECM™ 630 Exponential Decay Wave Electroporator with the following parameters ...
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bioRxiv - Genetics 2020Quote: Plasmid DNA containing the repair template with three tandem attP sites was co-transfected (1:1, Jetprime VWR#89129) with a plasmid containing Rosa26-Cas9/gRNA into mouse ES cells when confluency reached 70% ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies were produced by co-transfecting paired heavy- and light-chain antibody-expressing plasmids at a ratio of 1:1 using FectoPRO DNA Transfection Reagent (VWR) into Expi293 cells ...
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bioRxiv - Genetics 2019Quote: ... Four million S2 cells that were stably transduced with RasV12Common and RasV12Rare plasmids were seeded into coated tissue culture dishes (60×15mm, VWR) with 2 ml of Schneider’s media (without FBS) ...
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bioRxiv - Genetics 2021Quote: The 1.8 SRE-Elk cell line was generated by lentiviral transduction of 1.8 XX mESCs with the pLenti-SRE/Elk-GFP-PEST-Hygro plasmid (Supp. Table S4) followed by Hygromycin (250 ng/µl, VWR) selection ...
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bioRxiv - Molecular Biology 2023Quote: ... raw HEK cells were transfected with the respective plasmid by applying the jetPRIME transfection protocol (VWR International GmBH, Vienna, Austria). Adding 100 µl geneticin (G418 ...
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bioRxiv - Cell Biology 2020Quote: ... Injection mixture was prepared on ice containing 300 ng Tol2 transposase RNA and 500 ng recombinant plasmid and was brought to 10 µl total volume with 0.1% phenol-red (VWR, 470301-974) in water ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with retroviral Gag-pol (8 ug) and VSV-G (4 ug) packaging plasmids and pBabe vector (8 ug) using PEI Max transfection reagent (VWR, 75800-188). After 24 h ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmid DNA was quantitated using a NanoDrop spectrophotometer and diluted to 20 fmol/μL in nuclease-free water (VWR 02-0201-0500). An appropriate volume of reaction mastermix was prepared with the following composition ...
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bioRxiv - Neuroscience 2024Quote: ... 12 µg of plasmid DNA (ratio 1:10 for guide-RNA and dCas9-construct containing plasmids) was diluted into 5 µl of sterile 10 % glucose and added to diluted In vivo-jetPEI® reagent (#101000040 VWR, Polyplus-transfection). The solution was mixed by pipetting and incubated at RT for 15 min ...
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bioRxiv - Genomics 2023Quote: ... The resulting plasmid libraries were purified and concentrated via SPRI bead cleanup before being transformed into electrocompetent cells (Lucigen Endura, VWR International LLC #71003-038) for plasmid library amplification ...