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Citations for VWR :
1 - 50 of 182 citations for Plant DNA Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Total DNA was isolated using Plant DNA mini Kit (Peqlab/VWR, Darmstadt, Germany). To quantify fungal or bacterial DNA content ...
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bioRxiv - Genomics 2023Quote: DNA was purified using the yeast genomic DNA purification kit (VWR). Briefly ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We extracted the DNA using the Life Science Yeast Genomic DNA Purification Kit (VWR). We sent the samples for library preparation and sequencing at the Microbial Genome Sequencing Center (MiGS ...
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bioRxiv - Plant Biology 2021Quote: ... DNA was extracted from ∼50 mg samples with an E.Z.N.A® Plant DNA Kit (VWR, Lutterworth, UK). DNA fragment size was assessed on a 1% agarose gel in Tris/Borate/EDTA to confirm that all samples had the majority of DNA fragments >10 kilobases.
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bioRxiv - Plant Biology 2023Quote: Genomic DNA was extracted using the peqGOLD Plant DNA Mini Kit (PeqGold, VWR International GmbH, Darmstadt, Germany) for lines transformed with pOsCPR5.1-B construct while extraction was done according to (Edwards et al. ...
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bioRxiv - Plant Biology 2021Quote: Leaf genomic DNA was isolated using a peqGOLD Plant DNA Mini Kit (PeqGold, VWR International GmbH, Darmstadt, Germany) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The amplification conditions were adjusted using foliar DNA isolated with the Plant DNA mini kit (VWR, Darmstadt, Germany). Thus ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Single colonies were grown overnight in 10 mL YPGal+Dex and genomic DNA was isolated from 1 mL aliquots using the VWR® Life Science Yeast Genomic DNA Purification Kit (VWR 89492-616, us.vwr.com).
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bioRxiv - Evolutionary Biology 2023Quote: ... PCRs were carried out using the KAPA3G Plant PCR Kit (Kapa Biosystems, VWR) using the following cycling conditions for the phylogenetically informative loci ...
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bioRxiv - Genetics 2020Quote: ... Tissue DNA isolation kit (Omega Biotek, VWR). For fat pad and synovium ...
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bioRxiv - Genetics 2020Quote: ... DNA/RNA isolation kit (Omega Biotek, VWR). For blood ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was prepared from excised root tips (tip growth gained after seedling transfer) by using the peqGOLD Plant RNA Kit (VWR). One biological replicate represents 40-60 pooled root tips ...
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bioRxiv - Molecular Biology 2020Quote: ... Purification of Okazaki fragments was accomplished by running the denatured genomic DNA through 400 ul Source 15Q (VWR 89128-854), binding at 300 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... subtilis using the peqGOLD Bacterial DNA Kit (VWR). Plasmid DNA was isolated using the Nucleospin Extract Kit (Macherey-Nagel) ...
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bioRxiv - Genomics 2024Quote: The peqGOLD Tissue DNA Mini Kit (VWR Life Science) was used to extract genomic DNA after genome editing in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... DNA extracted using a PeqGold DNA Blood and Tissue Extraction kit following the manufacturer’s instructions (VWR), and PCR performed targeting both Ca ...
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bioRxiv - Plant Biology 2021Quote: ... 8 g/l) was prepared in plant boxes (125 ml, VWR, US). The agar samples were harvested from the boxes in which two plants were grown for three months under a log day (16h day/8h night ...
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bioRxiv - Genetics 2024Quote: ... The DNA was isolated from frozen ear muscle biopsies using the peqGOLD blood & tissue DNA mini kit (VWR) and following the protocol provided by the manufacturer.
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bioRxiv - Neuroscience 2021Quote: ... before purification with phenol:chloroform:isoamyl-alcohol 1 Phase (VWR), 5Prime Phase Lock Gel Heavy tube (Quantabio) ...
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bioRxiv - Plant Biology 2023Quote: ... frozen plant material was homogenized using the Precellys 24 (Peqlab Life Science, VWR) and proteins were extracted with 2x SDG buffer (62 mM Tris-HCL pH 8.6 ...
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bioRxiv - Plant Biology 2023Quote: ... the devices containing the plants were placed into Petri-dishes (391-0565, VWR, Spain) filled with 40 mL of distilled water ...
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bioRxiv - Bioengineering 2021Quote: ... treatments were applied by transferring the plants to 0.7% (m/v) agarose (VWR Life Sciences) with 1.5 mM MES buffers (Duchefa ...
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bioRxiv - Plant Biology 2020Quote: ... plants were harvested and meristems were stained with 5 μM SynaptoRed membrane dye (VWR 80510-682) in DMSO ...
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bioRxiv - Genetics 2019Quote: ... DNA was extracted from the gel slices using the peqGOLD Gel Extraction Kit (VWR). Genotyping was done using a modified ast-PCR protocol for R21X (Supplemental methods ...
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bioRxiv - Plant Biology 2019Quote: 6x-His purification was conducted in 20 mL gravity columns (VWR) loaded with 4 mL HisPur™ Ni-NTA resin (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... single myoXIaWT-VAMP72A1 and myoXIaTS-VAMP72A1 plants were placed in a round 35 mm confocal dishes (VWR), on top of an agar pad containing latrunculin B (0.25 μM) ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid DNA was amplified and purified using EZNA endo-free plasmid maxi kit (VWR International).
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bioRxiv - Molecular Biology 2022Quote: ... DNA was digested according to the peqGOLD DNase I Digest kit (VWR 13-1091-01), followed by washing steps with 400 µL RNA Prep Buffer (ZymoResearch R1060-2 ...
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bioRxiv - Microbiology 2024Quote: Shotgun sequencing libraries were prepared using the Lucigen NxSeq AmpFREE Low DNA Library Kit (VWR International, Radnor, USA), and sequenced with PE150 on an Illumina NovaSeq6000.
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bioRxiv - Bioengineering 2020Quote: ... biofilm from the wet-end (BF) and the effluent from the biological treatment plant (BTP) were individually microbiologically enriched in M9 media (VWR, USA) (Miller ...
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bioRxiv - Plant Biology 2019Quote: ... DNA fragments were separated in 2% (w/v) agarose gels and purified using the peqGOLD Gel Extraction Kit (VWR). Heat-shock transformation of chemically competent Escherichia coli DH5α cells were performed for all vectors followed by selection on LB-agar plates with ampicillin as a selection marker ...
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bioRxiv - Synthetic Biology 2022Quote: ... Each plant was sprayed for one minute and the collected flow through from each plant were first strained using a 40 μm cell strainer (VWR 76327-098) to remove large microorganisms and large particles ...
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bioRxiv - Biophysics 2022Quote: ... LB broth and ampicillin for wild type (WT) α-Syn purification were purchased from VWR (Denmark). Purified Alexa-488 maleimide labeled 140C-α-Syn (24 μM stock solutions prepared in 10 mM sodium phosphate buffer (NaH2PO4.H20+Na2HPO4.2H20 ...
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bioRxiv - Immunology 2023Quote: ... genomic DNA was extracted from individual clones using QuickExtract DNA solution (VWR) per manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using prepGEM® Tissue kit according to a protocol adapted from the manufacturer (ZYGEPTI0500, ZyGEM, VWR International BV) and subjected to PCR using the primers listed in Supplemental Table S5 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We inoculated these 16 samples in YEPD for 12 hours and extracted their genomic DNA using a commercially available kit (Amresco, Inc. VWR USA). We measured the concentration of DNA with a Qubit fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Neuroscience 2023Quote: ... DNA from one well for each clone was isolated via QuickExtract DNA Extraction Solution (VWR) to be used for genotyping ...
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bioRxiv - Neuroscience 2021Quote: ... cells were transfected with 3 ug of DNA in the same superfolder GFP-containing pEG BacMam vector used for large-scale purification and 9 μL FuGene HD (VWR) diluted in 150 μL OptiMEM and added dropwise to the cells after a 5 min incubation ...
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bioRxiv - Microbiology 2024Quote: Plasmids and corresponding oligonucleotides used in this study are summarized in Supplementary Tables 1 and 2. For DNA preparations, the appropriate enzymes (Fermentas, St. Leon-Rot, Germany) and kits (VWR International GmbH, Darmstadt, Germany) were used ...
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bioRxiv - Molecular Biology 2022Quote: DNA was extracted from HEK-293 cells using DirectPCR® DNA Lysis Reagent (VWR, 732-3255) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... DNA and siRNA transfection reagents (VWR) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Worms were resuspended in 7 ml pre-chilled Nuclei Purification Buffer+ and dounced on ice in a Wheaton dura-grind stainless steel dounce tissue grinder (VWR 62400-675) for 12 strokes ...
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bioRxiv - Genomics 2019Quote: Cells were harvested and total genomic DNA was isolated using QuickExtract DNA Extraction Solution (VWR, Radnor, PA, cat# QE09050). PCR was prepared using 5X GoTaq Green Reaction Buffer and GoTaq DNA Polymerase (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... the DNA was digested with 2.5U Benzonase (VWR) for 30 min at RT and the resulting extract saved as “Chromatome fraction” ...
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bioRxiv - Genomics 2022Quote: ... DNA was extracted using phenol-chloroform (VWR, K169). Libraries were constructed from 10 ng purified DNA using the High Throughput Library Prep Kit from KAPA Biosystems (KK8234 ...
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bioRxiv - Molecular Biology 2024Quote: ... VWR® TEMPase Hot Start DNA Polymerase (VWR) was used at 0.9 unit / reaction in the presence of 2.5 mM MgCl2 ...
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bioRxiv - Neuroscience 2023Quote: ... Clarified lysate was warmed up at room temperature at least 12 hours prior to affinity purification and then sterile filtered using a vacuum bottle filter (VWR International LLC, 28199-778). AAVX and AAV9 columns were attached to an AKTA Pure 25 L chromatography system (Cytiva ...
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bioRxiv - Cancer Biology 2021Quote: ... #TRCN0000002866) with jetOPTIMUS® DNA transfection Reagent (VWR, #76299-632) following the protocol provided by the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... DNA was detected by staining the gel with GelRed (VWR). The same gel was afterwards stained with InstantBlue (Life Technologies ...