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Citations for VWR :
1 - 50 of 930 citations for Mouse Eukaryotic Translation Initiation Factor 4E Binding Protein 1 EIF4EBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... 0.45μm low protein binding membrane (#28145-479) was from VWR International ...
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bioRxiv - Cell Biology 2020Quote: ... the medium was removed and filtered through a 0.45-µm low-protein binding membrane (VWR International, Radnor, PA). McArdle-RH7777 or IMR-90 were then transduced with the virus with 8 µg/ml polybrene (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... culture medium was changed and lentivirus-containing supernatant was collected 48 hours after transfection and filtered through a 0.45 μm low-protein-binding filter (VWR). Target cells were plated in 6-well culture plates with a 60-80% confluency ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibody binding was visualized using the using BrightVision Goat anti Rabbit / Poly-AP and BrightVision Goat anti Mouse / Poly-HRP (VWR/ImmunoLogic), with DAB as chromogen (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2023Quote: ... Enzyme solutions for +M and -M conditions were prepared in separate 8-tube PCR strips (VWR #732-1521 or low-protein binding) by mixing 25 μL reaction buffer (+M/-M ...
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bioRxiv - Cell Biology 2019Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
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bioRxiv - Immunology 2023Quote: ... ELISA-grade plates (490012-252, VWR) were coated overnight at 4°C with 2 µg/mL of anti-mouse IgE (553413 ...
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bioRxiv - Cancer Biology 2021Quote: ... embedded in 100% growth factor-reduced Matrigel (VWR), plated in 24-well tissue culture plates as Matrigel domes and overlaid with growth medium (Boj et al. ...
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bioRxiv - Cell Biology 2020Quote: ... and 0.4 μg factor I (FI) (VWR International,) in PBS (total volume of 20 μl ...
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bioRxiv - Molecular Biology 2022Quote: ... and anti-mouse (1:10,000; VWR)-conjugated to horseradish peroxidase HRP.
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bioRxiv - Neuroscience 2021Quote: ... in sterile PBS for ELISA (VWR #L0119) or TRI Reagent (Zymo ...
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bioRxiv - Pathology 2019Quote: ... total iron binding capacity (TIBC) and transferrin saturation were measured using the Stanbio Iron & TIBC kit (VWR, cat. number 10152-550). Serum ferritin was quantified using the mouse ferritin ELISA kit (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... protein concentrations were measured using a Bradford kit (VWR). The lysate and supernatant samples were mixed with 1x Laemmli buffer with 1.5% 2-mercaptoethanol and were denatured at 95 ºC for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... Antibody binding was visualized with DAB-nickel (VWR, 101098-434). Sections were counterstained with nuclear fast red ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), mouse anti-NeuN (Sigma # MAB377 ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), rabbit anti-dsRed (Takara #632496 ...
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bioRxiv - Biophysics 2023Quote: ... and GAPDH (1:5000, 101983-284 mouse, VWR). Membranes were then washed and incubated with secondary antibodies for 1 hour (both 1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... goat-α-mouse-CF568 (1:500, VWR; AB_10559187), and goat-α-guinea pig-AlexaFluor647 (1.500 ...
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bioRxiv - Biophysics 2023Quote: ... The concentration of total proteins was determined with the BCA Protein Assay Kit (VWR International GmbH, Darmstadt, Germany). According to the total protein amount in each sample ...
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bioRxiv - Biophysics 2021Quote: ... Cell culture media growth factors and antibodies were purchased from VWR International PBI (Milan ...
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bioRxiv - Cancer Biology 2019Quote: Corning Growth factor-reduced Matrigel was purchased from VWR (#734-1101). Cell culture media were purchased from Invitrogen Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... mouse monoclonal anti-GAPDH (VWR 1E6D9; 1:5,000 dilution), rabbit polyclonal anti-ACAT1 (Cell Signaling 44276 ...
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bioRxiv - Biochemistry 2023Quote: ... mouse anti-HIS his.h8 monoclonal (1:1000 dilution, VWR #101981-852); goat HRP-anti-rabbit and goat HRP-anti-mouse (1:1000 dilutions ...
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bioRxiv - Cell Biology 2022Quote: ... cAMP was measured using Cayman ELISA cAMP assay (VWR, Cat #75817-364) following manufacturer recommendations ...
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bioRxiv - Immunology 2023Quote: Greiner-Bio One 96 well half-area ELISA plates (VWR, 738-0032) were coated overnight at 4°C with 2 µg/ml CD40 protein (ThermoFisher ...
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bioRxiv - Biophysics 2022Quote: ... the purified and concentrated protein was incubated with 1:3 protein to biotin ratio of 1mM NHS-PEG4-Biotin (VWR #PI21362) for 1 hr at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... using the mouse MF20 (1:500, DSHB), the rabbit anti-GFP (1:1000, Life technology) and the rabbit RFP (1:1000, VWR) primary antibodies ...
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bioRxiv - Immunology 2023Quote: ELISAs were performed in a 96-well flat bottom polystyrene plate (VWR, 4394554). Plates were washed using a Tecan Hydroflex Plate Washer and read using a Thermo Scientific Multiskan FC ...
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bioRxiv - Neuroscience 2022Quote: ... then incubating in 5% normal horse serum for 60 min to block nonspecific binding (VWR, 102643-676). Afterwards ...
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bioRxiv - Cancer Biology 2020Quote: ... Successively the endogenous peroxidases and non-specific binding sites were blocked using 3% H2O2 (VWR chemicals, 23615.248) and Protein-Block solution (DAKO Agilent technologies ...
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bioRxiv - Biochemistry 2023Quote: ... protein expression was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG, #437145X, VWR) at 16 °C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... a competitive ELISA cAMP assay was used (Enzo Cat #581001 or VWR Cat #75817-364). Cells expressing bPAC constructs were plated on 6-well plates ...
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bioRxiv - Immunology 2021Quote: Human IgM antibodies were detected in sera from passively transferred mice using high-binding 384-well plates (VWR) coated with 0.5 µg/well of goat anti-human Ig polyvalent antibody (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse anti-cavin3 (Cat no. ABNOH00112464-MO4, 1:200 PLA, VWR International Pty, Australia), rabbit anti-BRCA1 C-20 (SC-642 ...
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bioRxiv - Cell Biology 2019Quote: ... The donkey anti-rabbit (NA934, 1:1000) and anti-mouse (NA931, 1:1000) horseradish peroxidase (HRP)-linked secondary antibodies were purchased from VWR. The GFER rabbit polyclonal antibody (#HPA041227 ...
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bioRxiv - Biochemistry 2021Quote: ELISAs were performed in 96-well Greiner LUMITRAC-600 white plates (VWR, cat. no. 82050-724). ELISAs were performed as indirect ELISAs ...
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bioRxiv - Cell Biology 2020Quote: ... After 1h binding on a wheel at 4°C beads were washed 3x in PBS supplemented with 0.02% Tween20 (VWR, M147) and 1% SDS and eluted with NuPAGE LDS buffer supplemented with 50 mM DTT and 1 mM biotin at 95 °C for 10 min ...
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bioRxiv - Genetics 2022Quote: ... A nonspecific antibody binding was prevented by an incubation of the fixed embryos in 3% BSA (VWR Life Science, 422351S) in PBS-T (1h ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoblots were then washed with TBS containing 0.05% Tween 20 (TBST) and incubated for 1 hour at ambient temperature with either anti-mouse horseradish peroxidase conjugated (NA931, VWR; 1:5000); or anti-rabbit HRP conjugated (NA934 ...
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bioRxiv - Biochemistry 2023Quote: ... at which point protein expression was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG) (VWR, #437145X) at the final concentration of 0.5 mM ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
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bioRxiv - Bioengineering 2019Quote: CC3 iPSCs[21] were maintained in E8 medium on standard tissue culture plastic plates coated with growth-factor reduced Matrigel (VWR). At 60-70% confluency ...
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bioRxiv - Neuroscience 2023Quote: ... PSCs from 80% confluent wells of a 6-well plate were plated onto a 12-well plate previously coated with reduced growth factor matrigel (VWR) in E8 media (d0 ...
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bioRxiv - Immunology 2022Quote: 3 µg/ml of spike antigens were coated onto the 384-well ELISA plates (VWR, Cat # 82051-300) overnight at 4 degree ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were probed with mouse anti-α-tubulin antibody (DM1A, VWR; #PI62204; 1:1000 in blocking buffer) for 1h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Protein bands were visualized using Blue BANDitTM protein stain (VWR Life Science, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227), and incubated at 100L°C for 10Lmin under reducing conditions ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 50 ng/ml recombinant murine macrophage-colony stimulating factor (rmM-CSF, Genentech)] and plated in 15-cm non-TC treated dishes for 5 days (Petri dish, VWR, 25384-326). Fresh BMDM media was added on day 3 without removal of original media ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins production was induced with 1 mM [F1] IPTG 3h [F2] treatment (Isopropil-β-D-tiogalattopiranoside, VWR, Pennsylvania, USA). Then ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then rinsed 3x in TBS and incubated for 1 hour in anti-mouse IR680 (VWR 102971-020) and anti-rabbit IR800 (VWR 103011-494 ...