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Citations for VWR :
1 - 50 of 72 citations for IL 17A Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: HEK293 cells were plated onto 12-mm glass coverslips (VWR) in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells were plated on sterilised 24 mm coverslips (VWR) at a low density to allow for single cells to be measured and clear background regions were available to record ...
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bioRxiv - Molecular Biology 2022Quote: HCT116 and HEK293 cells were seeded on 1.5mm coverslips (VWR) 24 hours prior to transfection with pLEICS25 wild-type or mutant GFP-Sam68 ...
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bioRxiv - Biophysics 2022Quote: ... His-bind resin was purchased from VWR.
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bioRxiv - Genetics 2023Quote: ... compound (VWR, Batavia, IL) and frozen in a bath of 2-methylbutane cooled with liquid nitrogen ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Plant Biology 2019Quote: 6x-His purification was conducted in 20 mL gravity columns (VWR) loaded with 4 mL HisPur™ Ni-NTA resin (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... mouse anti-HIS his.h8 monoclonal (1:1000 dilution, VWR #101981-852); goat HRP-anti-rabbit and goat HRP-anti-mouse (1:1000 dilutions ...
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bioRxiv - Bioengineering 2024Quote: HEK293 cells were seeded at 180,000 cells per well in a 24-well tissue culture plate (VWR) containing 500 µl cell culture media 24 hours prior to transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... DAB (Bright-DAB Substract Kit, IL ImmunoLogic, VWR) was used for protein detection ...
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bioRxiv - Developmental Biology 2022Quote: ... Cryosections (10 µm thickness) were collected on His-bond glass slides (VWR) and processed following (Jacobson et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10% fetal bovine serum (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (Gibco ...
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Pleiotropy in FOXC1-attributable phenotypes involves altered ciliation and cilia-dependent signalingbioRxiv - Genetics 2020Quote: ... embedded in Clear Frozen Section Compound (VWR, Richmond, IL) and frozen in a dry ice bath ...
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bioRxiv - Evolutionary Biology 2023Quote: ... containing 6-His tag and TEV cleaving site.19 In 50 mM HEPES (VWR, PA, USA), pH 7.5 including 50 mM potassium sulfate (Honeywell ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane was blocked with 5% bovine serum albumin (VWR, Batavia, IL) in PBS containing 0.1% Tween-20 for one hour at room temperature and then incubated in primary antibodies overnight at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant human TNF-alpha (PeproTech/VWR) was used at a concentration of 10 ng/ml.
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bioRxiv - Cell Biology 2020Quote: ... Pipettes were manufactured by pulling 100 μL capillary tubes (VWR, Arlington Heights, IL) to a tip diameter of ~0.5 micron (tip resistance = 1-5 MΩ ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were dried in an oven (INCU Line ILS 6, VWR, Radnor, PA, USA) at 50°C over 5 days and size sorted with a vibratory sieve shaker (AS 300 control ...
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Microbiology 2021Quote: ... 5 × 105 cells were cytospun onto 22 × 60 mm #1.5 coverslips (VWR international, Batavia, IL), pre-coated with CellTak (Corning) ...
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bioRxiv - Neuroscience 2023Quote: ... on a Leica cryostat and directly transferred to slides (Superfrost Plus; VWR International, Arlington, IL). Sections of brains ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL of each rescuer strain was inoculated into 500 μL of SC -his media in 96 well 2 mL deep well plate format (VWR) and shaken at 900 rpm at 30°C ...
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bioRxiv - Developmental Biology 2024Quote: ... 100ng/mL recombinant human Noggin (VWR International; 120-10C), 100ng/mL recombinant human FGF10 (Peprotech ...
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bioRxiv - Bioengineering 2021Quote: ... The solution was then transferred into 12-14 kDa MWCO dialysis membranes (VWR, Chicago, IL, USA) and dialyzed against DI water for one week ...
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bioRxiv - Microbiology 2021Quote: ... 100 μL of HRP-labelled Rabbit Anti-Sheep IgG (H+L) secondary antibody (VWR, Batavia, IL, USA) diluted 1:1000 (100ng/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... size fractions were shaken overnight at 56 °C using the incubation chamber INCU Line ILS 6 (VWR). After the lysate cooled down ...
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bioRxiv - Bioengineering 2021Quote: ... On day 3 the cells were collected using Trypsin ethylenediamine tetraacetic acid (EDTA) (VWR, Chicago, IL, USA). Cells were mixed with either GelMA (10% w/v final ...
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bioRxiv - Cell Biology 2021Quote: Human MSCs were fixed with 4% paraformaldehyde (PFA, VWR International) in deionized (DI ...
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bioRxiv - Biochemistry 2022Quote: ... The human cell lines HEK293T were maintained in DMEM (VWR) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2021Quote: The differentiated human iPSCs were fixed on glass coverslips (VWR, Denmark) in 4% PFA for 15 min at RT and stored at 4°C until use ...
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bioRxiv - Cell Biology 2024Quote: ... P/S and 2% human serum (VWR/BioWest, cat. #S4190-100). The culture insert’s apical site was supplemented with 300uL and the basolateral site with 800uL media ...
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bioRxiv - Microbiology 2021Quote: ... 100 μL of HRP-labelled Rabbit Anti-Deer IgG (H+L) secondary antibody (95058-328, VWR, Batavia, IL, USA) diluted 1:1000 (100ng/mL ...
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bioRxiv - Microbiology 2021Quote: Pooled normal human plasma was obtained from Pacific Hemostasis (VWR #95059-698). Factor X and prothrombin-deficient plasma were obtained from Haematologic Technologies (#FX-ID and #FII-ID) ...
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bioRxiv - Immunology 2020Quote: ... or Fc.IL-2 for 15 min at 37°C in complete RPMI (RPMI (Hyclone) supplemented with 10% FBS (heat inactivated, VWR), L-glutamine ...
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bioRxiv - Immunology 2022Quote: ... Human colon tissue samples were fixed in 4%(w/v) PFA (VWR International) for 24h at RT ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Two days post-transfection cells (20,000 cells/well, 7.5 μL) were seeded in low volume Greiner 384-well plates (#82051-458, VWR, Batavia, IL, USA) and were incubated with Glosensor reagent (Promega ...
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bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human alveolar macrophages (AMs) were lysed in RIPA buffer (VWR, Radnor, PA, USA) supplemented with protease (Roche Diagnostics GmbH ...
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bioRxiv - Biochemistry 2022Quote: ... venous blood samples had been collected and serum separated and saved in 0.5-1ml aliquots in cryovials (Nalgene Cryogenic tubes, VWR International, Batavia, IL, USA) and preserved at −80°C until batch analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... DAOY human medulloblastoma cell line was obtained from ATCC and was cultured in EMEM (VWR) supplemented with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... 10^5 human SHSY-5Y cells seeded on 18 mm #1 round cover glass (VWR) in a 12 well culture plate ...
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bioRxiv - Neuroscience 2020Quote: Snap frozen human DRGs were sectioned onto superfrost plus glass slides (VWR International, Leuven, Belgium) at 10μm thickness ...
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bioRxiv - Neuroscience 2022Quote: Human postmortem frozen brain tissue was embedded in Optimum Cutting Temperature medium (VWR,25608-930) and sectioned on a Leica cryostat at −17 C at 10 um onto Vizgen MERSCOPE coverslips (VIZGEN 2040003) ...
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bioRxiv - Neuroscience 2022Quote: Human postmortem frozen brain tissue was embedded in Optimum Cutting Temperature medium (VWR,25608-930) and sectioned on a Leica cryostat at −17 C at 10 um onto Vizgen MERSCOPE coverslips (VIZGEN 2040003) ...