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Citations for VWR :
1 - 50 of 105 citations for IGFBP 7 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells and HEK-293 cells were cultured on 22×22mm glass coverslips (VWR) in 6-well plates (Falcon) ...
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bioRxiv - Molecular Biology 2022Quote: DNA was extracted from HEK-293 cells using DirectPCR® DNA Lysis Reagent (VWR, 732-3255) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... We obtained HEK-293 cells from Jong Yun Lab (Penn State College of Medicine) and cultured them in DMEM (VWR International) supplemented with 10% fetal bovine serum (Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... mouse anti-HIS his.h8 monoclonal (1:1000 dilution, VWR #101981-852); goat HRP-anti-rabbit and goat HRP-anti-mouse (1:1000 dilutions ...
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Cell culture dimensionality influences mesenchymal stem cell fate through cadherin-2 and cadherin-11bioRxiv - Cell Biology 2019Quote: ... into HEK-293T cells using PEIpro (VWR) transfection reagent ...
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bioRxiv - Biophysics 2022Quote: ... His-bind resin was purchased from VWR.
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bioRxiv - Cell Biology 2023Quote: ... HEK 293T cells were cultured in DMEM with 10% FBS(VWR), NEAA (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... HEK-293T cells (ATCC) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, VWR) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... dechorionated in 7% Sodium Hypochlorite solution (VWR) and washed extensively with MilliQ water ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Plant Biology 2019Quote: 6x-His purification was conducted in 20 mL gravity columns (VWR) loaded with 4 mL HisPur™ Ni-NTA resin (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... or xylene (VWR, Randor, VA, 1330-20-7) and rehydrated in a series of graded ethanol washes (100% ...
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bioRxiv - Bioengineering 2024Quote: ... OTMs on day 3 or day 7 post-inoculum (day 10/14 post-seeding) were punched into 7 mm discs using a biopsy punch (VWR, Philadelphia) to fit into a Transwell insert (#29442-082 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cryosections (10 µm thickness) were collected on His-bond glass slides (VWR) and processed following (Jacobson et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10% fetal bovine serum (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (Gibco ...
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bioRxiv - Microbiology 2021Quote: HEK 293T cells (3×105 cells) were plated on the sterilized round 15mm #1 glass coverslips (VWR) in 12-well plates and seeded for 24hours prior to transfection ...
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bioRxiv - Developmental Biology 2019Quote: ... Pancreas sections (7 μm thick) mounted on polysine slides (VWR) were de-waxed ...
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bioRxiv - Developmental Biology 2022Quote: ... 100ul of deionized Formamide (VWR Catalog # CAS-75-12-7) was added to 900ul of hybridization buffer (Stellaris ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK T-REx cells were transfected with pcDNA4/TO/hTRPC3 using jetPRIME ® transfection reagent (VWR, Lutterworth, UK) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... pH 7) plus 0.3 mg/liter methylene blue (VWR, Radnor, PA) to prevent microbial growth for the first 6 hours ...
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bioRxiv - Biochemistry 2021Quote: For transductions VeroE6 or HEK cells were seeded into greiner CELLSTAR® 96-well plates or 6-well plates (VWR) containing lentivirus in DMEM containing 2 % FBS and 1 μg/mL polybrene ...
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bioRxiv - Molecular Biology 2023Quote: ... raw HEK cells were transfected with the respective plasmid by applying the jetPRIME transfection protocol (VWR International GmBH, Vienna, Austria). Adding 100 µl geneticin (G418 ...
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bioRxiv - Biophysics 2020Quote: ... Embryos were dechorionated by short immersion in 7% sodium hypochlorite solution (VWR). After extensive rinsing with water ...
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bioRxiv - Cell Biology 2020Quote: ... The embryos or eggs were dechorionated in 7% Sodium Hypochlorite solution (VWR), washed thoroughly in milliQ water ...
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bioRxiv - Biophysics 2023Quote: ... Embryos were dechorionated by short immersion in 7% sodium hypochlorite solution (VWR). After extensive rinsing with water ...
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bioRxiv - Genetics 2019Quote: HEK 293T cells were cultured in six-well plates and double transfected using jetPRIME transfection reagent (VWR International, Radnor, PA, USA) with pCMV-luciferase ...
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bioRxiv - Evolutionary Biology 2023Quote: ... containing 6-His tag and TEV cleaving site.19 In 50 mM HEPES (VWR, PA, USA), pH 7.5 including 50 mM potassium sulfate (Honeywell ...
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bioRxiv - Genetics 2021Quote: ... Staining was performed with 10µM H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate, VWR #89138-260) for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... transferred it to a 7 kilodalton cut-off SnakeSkinTM dialysis tubing (PierceTM, VWR), added Ulp1 protease [0.5 μM final concentration] and let it dialyze overnight at 4 °C in buffer A (with 7% glycerol ...
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bioRxiv - Neuroscience 2020Quote: ... injections of tamoxifen (100 mg/kg, dissolved in corn oil, VWR, #9001-30-7). The expression of GCaMP6f was verified by immunohistochemistry (with antibodies targeting GFP and neural marker NeuN).
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bioRxiv - Microbiology 2022Quote: ... scintillation vials (7 ml and 20 ml) and Coumarin C6 were purchased from VWR International (Radnor ...
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bioRxiv - Microbiology 2023Quote: ... the wells were overlaid with 2 mL/well of 7% Formaldehyde (VWR,10790-708) for 30 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Biophysics 2021Quote: ... The cells were left for 7 days and then fixed using 100% methanol (MeOH) (VWR, BDH2029) and stained with a crystal violet (Sigma ...
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bioRxiv - Neuroscience 2022Quote: Primary microglia were plated at 7×106 cells/well in 6-well plates (VWR #10062-894) and incubated at 5% CO2 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL of each rescuer strain was inoculated into 500 μL of SC -his media in 96 well 2 mL deep well plate format (VWR) and shaken at 900 rpm at 30°C ...
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bioRxiv - Cell Biology 2021Quote: First trimester placental villous tissue (7-10 weeks of gestation) was snap-frozen in OCT compound (VWR) and 5µm cryosections of tissue were cut and fixed in ice-cold acetone for 5 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... 7-day-old seedlings grown on hydroponic media were mounted on coverslips (24×50 mm No. 1½, VWR) using medical adhesive (Stock No ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was homogenized in a 2 ml lysis buffer using a Dounce tissue grinder (7 ml, VWR). After addition of a 1.8 M sucrose solution (4 mL) ...
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bioRxiv - Cell Biology 2022Quote: ... were grown to O.D 0.3-0.6 and incubated with non-fluorescent Halo tag ligand 7-bromo-1-heptanol (7BRO, 100μM, VWR #AAH54762) for 10 min to irreversibly mask all the pre-existing ribosomal proteins ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then subcultured 1:1000 into 7 mL of MSgg in a 6-well polyvinyl plate (VWR, PA, USA). Plates were incubated in static conditions at 30°C for 3 days and were then imaged using a Leica MZ10F macroscope and Leica DMC2900 camera ...
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bioRxiv - Physiology 2020Quote: ... Sections were then post-fixed in 4% PFA/PBS before washing with PBS/Tween 0.1% and then acetylated (1.325% triethanolamine [pH 7-8]; 0.25% acetic anhydride; 0.175% HCl in autoclaved water; VWR Chemicals, Lutterworth, UK) for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... Cryosections were cut in OCT at 7 μm thickness on Epredia CryoStar NX50 at -20°C chamber temperature and mounted onto lysine-coated Superfrost slides (VWR). Sections were fixed for 20 min in 4% ParaFormAldehyde (PFA ...
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bioRxiv - Plant Biology 2023Quote: ... the first chromatography was performed using a 7-cm-diameter column loaded with P60 silica gel (SiliCycle) and using hexane (HPLC grade; VWR) and ethyl acetate as the mobile phases ...
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bioRxiv - Plant Biology 2023Quote: ... Flash chromatography was carried out using a 7 cm diameter column loaded with 20 g of P60 silica gel (SiliCycle) with hexane (HPLC grade; VWR) as the mobile phase ...
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bioRxiv - Cancer Biology 2024Quote: ... cell lines were obtained and cultured as previously described (7) in RPMI-1640 basal media supplemented with 10% (15% for U266B1 cells) fetal bovine serum (FBS, VWR) with 1% antibiotic-antimycotic (ThermoFisher Scientific ...