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Citations for VWR :
1 - 50 of 146 citations for HLA A*0201 GP100 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... and 20-X μL nuclease free water (VWR 02-0201-0500). Reactions were run on a thermocycler with the following settings:
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmid DNA was quantitated using a NanoDrop spectrophotometer and diluted to 20 fmol/μL in nuclease-free water (VWR 02-0201-0500). An appropriate volume of reaction mastermix was prepared with the following composition ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Neuroscience 2019Quote: HEK293 cells were plated onto 12-mm glass coverslips (VWR) in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells were plated on sterilised 24 mm coverslips (VWR) at a low density to allow for single cells to be measured and clear background regions were available to record ...
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bioRxiv - Molecular Biology 2022Quote: HCT116 and HEK293 cells were seeded on 1.5mm coverslips (VWR) 24 hours prior to transfection with pLEICS25 wild-type or mutant GFP-Sam68 ...
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bioRxiv - Biophysics 2022Quote: ... His-bind resin was purchased from VWR.
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bioRxiv - Biochemistry 2019Quote: ... The crosslinked complex was then concentrated on a microcentrifuge filter (VWR 82031354) to < 20 μL and washed three times with 800 μL 8 M Urea ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Plant Biology 2019Quote: 6x-His purification was conducted in 20 mL gravity columns (VWR) loaded with 4 mL HisPur™ Ni-NTA resin (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... mouse anti-HIS his.h8 monoclonal (1:1000 dilution, VWR #101981-852); goat HRP-anti-rabbit and goat HRP-anti-mouse (1:1000 dilutions ...
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bioRxiv - Bioengineering 2024Quote: HEK293 cells were seeded at 180,000 cells per well in a 24-well tissue culture plate (VWR) containing 500 µl cell culture media 24 hours prior to transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... Cryosections (10 µm thickness) were collected on His-bond glass slides (VWR) and processed following (Jacobson et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10% fetal bovine serum (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... assuming a stoichiometry of Rad6⚫(Rad18)241 and the concentration of Rad6 within the complex was confirmed by Bradford assay using BSA (VWR) as a standard ...
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bioRxiv - Evolutionary Biology 2023Quote: ... containing 6-His tag and TEV cleaving site.19 In 50 mM HEPES (VWR, PA, USA), pH 7.5 including 50 mM potassium sulfate (Honeywell ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant human TNF-alpha (PeproTech/VWR) was used at a concentration of 10 ng/ml.
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL of each rescuer strain was inoculated into 500 μL of SC -his media in 96 well 2 mL deep well plate format (VWR) and shaken at 900 rpm at 30°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
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bioRxiv - Developmental Biology 2024Quote: ... 100ng/mL recombinant human Noggin (VWR International; 120-10C), 100ng/mL recombinant human FGF10 (Peprotech ...
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bioRxiv - Biochemistry 2022Quote: ... Protein bands were visualized using Blue BANDitTM protein stain (VWR Life Science, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227), and incubated at 100L°C for 10Lmin under reducing conditions ...
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bioRxiv - Cell Biology 2021Quote: Human MSCs were fixed with 4% paraformaldehyde (PFA, VWR International) in deionized (DI ...
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bioRxiv - Biochemistry 2022Quote: ... The human cell lines HEK293T were maintained in DMEM (VWR) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2021Quote: The differentiated human iPSCs were fixed on glass coverslips (VWR, Denmark) in 4% PFA for 15 min at RT and stored at 4°C until use ...
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bioRxiv - Cell Biology 2024Quote: ... P/S and 2% human serum (VWR/BioWest, cat. #S4190-100). The culture insert’s apical site was supplemented with 300uL and the basolateral site with 800uL media ...
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bioRxiv - Microbiology 2021Quote: Pooled normal human plasma was obtained from Pacific Hemostasis (VWR #95059-698). Factor X and prothrombin-deficient plasma were obtained from Haematologic Technologies (#FX-ID and #FII-ID) ...
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bioRxiv - Biophysics 2023Quote: ... The concentration of total proteins was determined with the BCA Protein Assay Kit (VWR International GmbH, Darmstadt, Germany). According to the total protein amount in each sample ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein was estimated using Bradford reagent (VWR).
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred to BiotraceNT membrane (VWR) and incubated with primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... and peqGOLD Protein-Marker I Unstained (VWR) were used as protein markers ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant protein extracts were recovered by centrifugation and protein concentration in samples was determined using Bradford Reagent (VWR).
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bioRxiv - Immunology 2022Quote: ... Human colon tissue samples were fixed in 4%(w/v) PFA (VWR International) for 24h at RT ...
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bioRxiv - Microbiology 2020Quote: ... Each fraction was resuspended in HEPES-NaCl buffer and 10 μL of each fraction was examined for protein concentration and lipid content using a Bradford protein assay (VWR) and the lipophilic FM4-64 dye (Molecular Probes) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein concentration was estimated by Bradford reagent (VWR) using bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2021Quote: ... Protein concentration was estimated by Bradford reagent (VWR) using bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2021Quote: ... The protein was incubated overnight with TEV (VWR) at 4°C and Histag and TEV was purified out with Ni-NTA beads (GE) ...
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bioRxiv - Biophysics 2022Quote: ... the purified and concentrated protein was incubated with 1:3 protein to biotin ratio of 1mM NHS-PEG4-Biotin (VWR #PI21362) for 1 hr at room temperature ...
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bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human alveolar macrophages (AMs) were lysed in RIPA buffer (VWR, Radnor, PA, USA) supplemented with protease (Roche Diagnostics GmbH ...
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bioRxiv - Biochemistry 2022Quote: ... A total amount of 15 μg protein was used for SDS-PAGE separation by electrophoresis and proteins were transferred onto nitrocellulose membranes (VWR, Lutterworth, UK). Even protein loading and transfer were confirmed by Revert 700 total protein staining (Li-COR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the proteins were diluted in 50 mM HEPES (VWR), pH 7.5 supplemented with 50 mM potassium sulphate (Honeywell ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: Protein extraction was performed with peqGOLD TriFast reagent (VWR), and proteins were separated by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were precipitated with 20% trichloroacetic acid (TCA – VWR) on ice overnight at 4° C ...