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Citations for VWR :
1 - 50 of 105 citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... individual fish were held in 473 mL white plastic cups with lids (VWR Multipurpose Containers ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We then fixed the fish in 10% neutral buffered formalin (VWR, Radnor, Pennsylvania). To facilitate gill raker counts ...
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bioRxiv - Physiology 2021Quote: Testes from all sampled fish were dissected and fixated in 4 % glutaraldehyde (VWR, Radnor, USA) overnight at 4 °C ...
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bioRxiv - Developmental Biology 2019Quote: Fish were anesthetized with tricaine and initially fixed by immersion in 4% paraformaldehyde (VWR:15713-S) /0.1M sodium-cacodylate (VWR:11653) ...
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bioRxiv - Genetics 2022Quote: ... and an equal volume of 1x Sperm Salts with 3% paraformaldehyde was applied for 5 min before samples were affixed to a Superfrost Plus slide (VWR). For both protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... Stellaris® RNA FISH Wash Buffer B was removed and samples were resuspended in Vectashield Antifade Mounting Medium with DAPI (VWR; Cat #: H-1200-10). Samples were then placed on a slide and sealed with a coverslip and nail polish.
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bioRxiv - Genomics 2023Quote: DNA was purified using the yeast genomic DNA purification kit (VWR). Briefly ...
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bioRxiv - Immunology 2023Quote: ... genomic DNA was extracted from individual clones using QuickExtract DNA solution (VWR) per manufacturer instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Total DNA was isolated using Plant DNA mini Kit (Peqlab/VWR, Darmstadt, Germany). To quantify fungal or bacterial DNA content ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We extracted the DNA using the Life Science Yeast Genomic DNA Purification Kit (VWR). We sent the samples for library preparation and sequencing at the Microbial Genome Sequencing Center (MiGS ...
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bioRxiv - Neuroscience 2023Quote: ... DNA from one well for each clone was isolated via QuickExtract DNA Extraction Solution (VWR) to be used for genotyping ...
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bioRxiv - Molecular Biology 2022Quote: DNA was extracted from HEK-293 cells using DirectPCR® DNA Lysis Reagent (VWR, 732-3255) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... DNA extracted using a PeqGold DNA Blood and Tissue Extraction kit following the manufacturer’s instructions (VWR), and PCR performed targeting both Ca ...
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bioRxiv - Systems Biology 2021Quote: ... DNA and siRNA transfection reagents (VWR) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... DNA was extracted from ∼50 mg samples with an E.Z.N.A® Plant DNA Kit (VWR, Lutterworth, UK). DNA fragment size was assessed on a 1% agarose gel in Tris/Borate/EDTA to confirm that all samples had the majority of DNA fragments >10 kilobases.
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bioRxiv - Plant Biology 2023Quote: Genomic DNA was extracted using the peqGOLD Plant DNA Mini Kit (PeqGold, VWR International GmbH, Darmstadt, Germany) for lines transformed with pOsCPR5.1-B construct while extraction was done according to (Edwards et al. ...
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bioRxiv - Plant Biology 2021Quote: Leaf genomic DNA was isolated using a peqGOLD Plant DNA Mini Kit (PeqGold, VWR International GmbH, Darmstadt, Germany) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... The DNA was isolated from frozen ear muscle biopsies using the peqGOLD blood & tissue DNA mini kit (VWR) and following the protocol provided by the manufacturer.
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bioRxiv - Molecular Biology 2024Quote: ... The amplification conditions were adjusted using foliar DNA isolated with the Plant DNA mini kit (VWR, Darmstadt, Germany). Thus ...
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bioRxiv - Genetics 2020Quote: ... Tissue DNA isolation kit (Omega Biotek, VWR). For fat pad and synovium ...
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bioRxiv - Genetics 2020Quote: ... DNA/RNA isolation kit (Omega Biotek, VWR). For blood ...
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bioRxiv - Genomics 2019Quote: Cells were harvested and total genomic DNA was isolated using QuickExtract DNA Extraction Solution (VWR, Radnor, PA, cat# QE09050). PCR was prepared using 5X GoTaq Green Reaction Buffer and GoTaq DNA Polymerase (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... the DNA was digested with 2.5U Benzonase (VWR) for 30 min at RT and the resulting extract saved as “Chromatome fraction” ...
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bioRxiv - Genomics 2022Quote: ... DNA was extracted using phenol-chloroform (VWR, K169). Libraries were constructed from 10 ng purified DNA using the High Throughput Library Prep Kit from KAPA Biosystems (KK8234 ...
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bioRxiv - Microbiology 2023Quote: ... subtilis using the peqGOLD Bacterial DNA Kit (VWR). Plasmid DNA was isolated using the Nucleospin Extract Kit (Macherey-Nagel) ...
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bioRxiv - Molecular Biology 2024Quote: ... VWR® TEMPase Hot Start DNA Polymerase (VWR) was used at 0.9 unit / reaction in the presence of 2.5 mM MgCl2 ...
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bioRxiv - Genomics 2024Quote: The peqGOLD Tissue DNA Mini Kit (VWR Life Science) was used to extract genomic DNA after genome editing in accordance with the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Single colonies were grown overnight in 10 mL YPGal+Dex and genomic DNA was isolated from 1 mL aliquots using the VWR® Life Science Yeast Genomic DNA Purification Kit (VWR 89492-616, us.vwr.com).
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bioRxiv - Cancer Biology 2021Quote: ... #TRCN0000002866) with jetOPTIMUS® DNA transfection Reagent (VWR, #76299-632) following the protocol provided by the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... DNA was detected by staining the gel with GelRed (VWR). The same gel was afterwards stained with InstantBlue (Life Technologies ...
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bioRxiv - Zoology 2022Quote: ... using EZ-Vision Bluelight DNA dye (VWR Life Sciences, USA), purified using ExoCleanUp FAST PCR clean-up reagent (VWR Life Sciences USA) ...
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bioRxiv - Bioengineering 2023Quote: ... or RedTaq DNA Polymerase 2X Master Mix (VWR Life Science) using primers designed in Geneious Prime 2023.1.2 (Supplementary Table 5) ...
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bioRxiv - Cell Biology 2019Quote: ... genomic DNA was prepared using DirectCell-PCR Lysis-Reagent Cell (VWR) according to the manufacturer’s protocol and screened by PCR using a FailSafe™ PCR kit (Buffer E ...
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bioRxiv - Cell Biology 2020Quote: Genomic DNA was extracted with direct PCR Lysis Reagent Cell (VWR). RNA isolation was performed using the AMBION PureLink RNA Mini kit (Themo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... and DNA was quantified on a mySPEC spectrophotometer (VWR®/ Germany). Whole metagenome sequencing was performed on a NovaSeq 6000 system ...
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bioRxiv - Biochemistry 2023Quote: ... DNA was extracted by Phase Lock Gel™ (VWR, 10847-800) per manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... A 100 to 3,000 base pairs DNA weight marker (VWR, USA) was employed for band sizing ...
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bioRxiv - Cell Biology 2024Quote: Genomic DNA was extracted using Direct PCR Lysis Reagent Cell (VWR). RNA was extracted using the AMBION PureLink RNA Mini Kit (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... and total DNA content measured using the fluorometric Quantifluor dsDNA Assay (VWR) and converted to cell number for normalization.
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bioRxiv - Cell Biology 2023Quote: ... DNA-RNA hybrids were eluted and treated with Proteinase K (VWR Scientific). Eluted DNA was subsequently isolated by phenol:chloroform:isoamyl alcohol extraction and DRIP-qPCR was performed on Myocardin promoter and control locus and results were calculated as per Sanz and Chédin ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were sorted into DNA LoBind® Eppendorf tubes (VWR, #525-0130), then CD45-cells were mixed with CD45+ cells at 2:1 to 10:1 ratios depending on cell availability to enrich for glioblastoma cells ...
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bioRxiv - Microbiology 2024Quote: ... The precipitated DNA was resuspended in TE buffer (VWR, Radnor, PE, USA) and purified using QIAamp DNA Mini Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2020Quote: ... genomic DNA was extracted by lysing with DirectPCR Lysis Reagent Cell (Peqlab, VWR) supplemented with 0.5 μg/μl Proteinase K (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The DNA pellet was washed with 70% ethanol (VWR, Cat. No. 71006-012) twice and air dried ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was precipitated by adding an equal volume of isopropanol (VWR, Radnor, PA) and incubated at −20°C for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... 0.33 mM of each dNTP and 1 U of Taq DNA polymerase (VWR) using one cycle of 94°C for 5 min 30 s ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was extracted from cortical tissue using the TriFast™ reagent protocol (VWR) and the Cacna1c gene was amplified with the PfuPlus! DNA Polymerase (Roboklon) ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA quality was examined via gel electrophoresis on a 0.5% agarose gel (VWR) with 0.1% GelRed (10000x ...
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bioRxiv - Plant Biology 2022Quote: ... PCR was performed with Red Taq DNA Polymerase Master Mix (VWR Life Science) according to the manufacturer’s instructions and purified with magnetic beads (CleanNGS) ...
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bioRxiv - Neuroscience 2022Quote: ... 0.33 mM of each dNTP and 1 U of Taq DNA polymerase (VWR) using one cycle of 94°C for 5 min 30 s ...