Labshake search
Citations for VWR :
1 - 50 of 843 citations for Dickkopf related protein 1 DKK 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... mouse anti-HIS his.h8 monoclonal (1:1000 dilution, VWR #101981-852); goat HRP-anti-rabbit and goat HRP-anti-mouse (1:1000 dilutions ...
-
bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
-
bioRxiv - Neuroscience 2023Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), mouse anti-NeuN (Sigma # MAB377 ...
-
bioRxiv - Neuroscience 2024Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), rabbit anti-dsRed (Takara #632496 ...
-
bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
-
bioRxiv - Neuroscience 2019Quote: HEK293 cells were plated onto 12-mm glass coverslips (VWR) in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega ...
-
bioRxiv - Biochemistry 2020Quote: HEK293 cells were plated on sterilised 24 mm coverslips (VWR) at a low density to allow for single cells to be measured and clear background regions were available to record ...
-
bioRxiv - Molecular Biology 2022Quote: HCT116 and HEK293 cells were seeded on 1.5mm coverslips (VWR) 24 hours prior to transfection with pLEICS25 wild-type or mutant GFP-Sam68 ...
-
bioRxiv - Molecular Biology 2020Quote: ... 10^5 human SHSY-5Y cells seeded on 18 mm #1 round cover glass (VWR) in a 12 well culture plate ...
-
bioRxiv - Molecular Biology 2021Quote: ... Both Jurkat and primary human T cells were treated with 100 μg ml-1 cycloheximide (VWR) 5 minutes before harvesting ...
-
bioRxiv - Biophysics 2022Quote: ... His-bind resin was purchased from VWR.
-
bioRxiv - Biophysics 2022Quote: ... the purified and concentrated protein was incubated with 1:3 protein to biotin ratio of 1mM NHS-PEG4-Biotin (VWR #PI21362) for 1 hr at room temperature ...
-
bioRxiv - Biochemistry 2023Quote: ... protein expression was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG, #437145X, VWR) at 16 °C overnight ...
-
bioRxiv - Cell Biology 2022Quote: Caco-2 cells or human ileum organoids were lysed in 1 mL 20 mM Suprapur NaOH (VWR International) for 2 hours ...
-
bioRxiv - Immunology 2020Quote: Cells of human or murine origin (0.2-1×106) were lysed in Trizol (peqGOLD TriFast™; VWR International, Radnor, USA) and total RNA was extracted by use of the DirectZol® RNA MiniPrep (Zymo Research ...
-
bioRxiv - Biochemistry 2023Quote: ... at which point protein expression was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG) (VWR, #437145X) at the final concentration of 0.5 mM ...
-
bioRxiv - Genomics 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
-
bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
-
bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
-
bioRxiv - Genomics 2020Quote: Single cell suspensions in PBS with 2% FBS were stained by incubating for 15 minutes at room temperature protected from light with anti-human PTPRC (CD45) monoclonal antibody conjugated to FITC (1:200 dilution, VWR #ABNOMAB12230), anti-human EPCAM antibody conjugated to PE (1:50 dilution ...
-
bioRxiv - Plant Biology 2019Quote: 6x-His purification was conducted in 20 mL gravity columns (VWR) loaded with 4 mL HisPur™ Ni-NTA resin (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2022Quote: ... Proteins production was induced with 1 mM [F1] IPTG 3h [F2] treatment (Isopropil-β-D-tiogalattopiranoside, VWR, Pennsylvania, USA). Then ...
-
bioRxiv - Plant Biology 2020Quote: 1-Aminocyclopropane-1-carboxylic acid (ACC; VWR P10007) was dissolved in sterile water (10 mM stock ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 1 (VWR) were cleaned by a 20 minute exposure in a 2.6 L Zepto plasma laboratory unit (Diener Electronic) ...
-
bioRxiv - Bioengineering 2024Quote: HEK293 cells were seeded at 180,000 cells per well in a 24-well tissue culture plate (VWR) containing 500 µl cell culture media 24 hours prior to transfection ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cryosections (10 µm thickness) were collected on His-bond glass slides (VWR) and processed following (Jacobson et al. ...
-
bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10% fetal bovine serum (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (Gibco ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were stored at 4°C while the incubator cooled to 18°C and then protein expression was induced with 1 mM IPTG (VWR) and expressed at 18°C overnight ...
-
bioRxiv - Cancer Biology 2023Quote: ... and Matrigel Basement Membrane Matrix (1:1) (354234, VWR).
-
bioRxiv - Neuroscience 2021Quote: ... Rabbit anti-S100 1:300 (VWR/ProteinTech 15146-1-AP), Chick anti-Neurofilament 200 kDa 1:500 (Aves NFH) ...
-
bioRxiv - Developmental Biology 2021Quote: ... 1% DMSO (VWR) and 0.2 mM 1-phenyl-2-thiourea (Acros Organics ...
-
bioRxiv - Cancer Biology 2019Quote: ... 1% BSA (VWR) and 0.1% Tween 20 (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2021Quote: ... the virus suspension was mixed 1:1 with peqGOLD TriFast (VWR). Following ...
-
bioRxiv - Molecular Biology 2023Quote: ... then neutralized with 1 μL 5 M HCl (VWR BDH7419-1). The remainder of the library preparation was performed according to the xGen protocol except that post-ligation ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and then 1 hour in 1:1 ratio of 30% sucrose/0.1M phosphate buffer:OCT (VWR, Cat No. 25608-930). Tissue was embedded inside a mold made by the severed end of a 1.5mL microcentrifuge tube attached to a glass slide with nail polish and stored at −80°C until used ...
-
bioRxiv - Molecular Biology 2021Quote: ... and G418 sulfate (500 μg mL−1, from 50 mg mL−1 stock in H2O, from 712 U mg−1 powder, VWR). Cells were checked monthly for mycoplasma contamination by PCR assay ...
-
bioRxiv - Developmental Biology 2023Quote: ... using the mouse MF20 (1:500, DSHB), the rabbit anti-GFP (1:1000, Life technology) and the rabbit RFP (1:1000, VWR) primary antibodies ...
-
bioRxiv - Biophysics 2020Quote: ... 1% (w/w) 10:1 (base/crosslinker w/w) Sylgard-184 (VWR) was added to the GEL-8100 and mixed well for 60 seconds ...
-
bioRxiv - Molecular Biology 2023Quote: ... Each sample was homogenised in 1 mol.L-1 HCl (VWR, United States). Free glucose content was measured at this stage ...
-
bioRxiv - Immunology 2023Quote: ... HOIP (VWR/Proteintech 16298-1-AP, SHARPIN (VWR/Proteintech 14626-1-AP), and actin (Sigma-Aldrich A5316 ...
-
bioRxiv - Cell Biology 2024Quote: ... Each sample was homogenized in 1 mol.L-1 HCl (VWR, United States) and free glucose content was measured ...
-
bioRxiv - Neuroscience 2023Quote: ... the tissue sections were co-stained overnight at 4°C with a primary chicken antibody against the astrocyte marker Glial Fibrillary Acidic Protein (GFAP) (1:1000 in blocking solution; Cat No. 200-901-D60, VWR, Radnor, PA, USA) and a primary rat antibody against myelin basic protein (MBP ...
-
bioRxiv - Genetics 2021Quote: ... 1 mM Squalene (VWR), 50 µM Lanosterol (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2021Quote: ... 1% BSA (421501J, VWR), 0.1% Triton-X and 0.05% Tween-20 in PBS ...
-
bioRxiv - Cell Biology 2020Quote: ... 1 mM MgCl2 (VWR), 1% Triton X-100 (Alfa Aesar) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1% penicillin-streptomycin (VWR, 5 units/mL penicillin ...
-
bioRxiv - Biochemistry 2023Quote: ... 1 mM dithiothreitol (VWR) in 384-well plates (Corning ...
-
bioRxiv - Biochemistry 2023Quote: ... 1 mM dithiothreitol (VWR) in 384-well plates (Corning ...
-
Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... 1 cover glass (VWR) was used for the top plate and a 40 mm cover glass (Fisherbrand ...
-
bioRxiv - Neuroscience 2023Quote: ... brains were kept for 1 hour in a 1:1 solution of 30% sucrose and Optimal Cutting Temperature Medium (OCT, VWR International) at 4°C ...