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Citations for VWR :
1 - 50 of 421 citations for Dengue Virus Subtype 4 Envelope 45 kDa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
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bioRxiv - Plant Biology 2023Quote: ... was added to deactivate the protein (45°C, 4 hours) and the DNA was purified through phase lock gel (VWR) and precipitated with 1/10 volume of 3 M Sodium Acetate (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... Thereafter proteins were recovered using 10 kDa spin filters (VWR), and samples were reduced ...
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bioRxiv - Bioengineering 2023Quote: ... stably expressing enhanced green fluorescent protein (eGFP) were generated as described before[45] and cultured on 0.1% gelatin (VWR) pre-coated surfaces in MCDB-131 medium (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... Matrigel containing organoid samples were removed from multi-well tissue culture dishes (using a 3.5 mL disposable transfer pipette and placed in 45 mL of cold PBS (4°C) in 50 mL capacity polypropylene centrifuge tubes (VWR Scientific). Matrigel was washed away by 3-4 rounds of suspension in cold PBS and centrifugation using a refrigerated centrifuge set to 1,000 pm ...
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bioRxiv - Physiology 2021Quote: ... Tissue samples were embedded in paraffin and sections of 4 μm were coated with 43–45°C Kodak NTB emulsion (VWR, Herlev, Denmark) diluted 1:1 with 43–45°C water ...
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bioRxiv - Biophysics 2020Quote: ... 1 kDa centrifugal filters (VWR).
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bioRxiv - Evolutionary Biology 2022Quote: ... The tube was topped off with 45:45:10 1xMMR:L15:FCS to inactivate the papain and strained threw a 70um sieve (VWR 10199-656). The sieve was washed with 25ml 45:45:10 1xMMR:L15:FCS ...
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bioRxiv - Cell Biology 2020Quote: ... Residual lipids and proteins were cleared from the extracts by spin filtration in 3-kDa-molecular-weight spin cut-off filter (VWR) added 3 mg C18-material (Waters) ...
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bioRxiv - Bioengineering 2024Quote: ... 45 mg/100 mL of D-Glucose (VWR, BDH9230). Chondrocytes of passage 4-8 were used in experiments.
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bioRxiv - Neuroscience 2019Quote: ... serum-free culture media containing secreted proteins were collected and concentrated in 0.5 mL 3-kDa molecular weight cut-off centrifugal filters (VWR North America). In some experiments ...
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bioRxiv - Cell Biology 2019Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
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bioRxiv - Microbiology 2021Quote: ... the virus suspension was mixed 1:1 with peqGOLD TriFast (VWR). Following ...
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bioRxiv - Molecular Biology 2023Quote: ... using a 60 min gradient of 4.5 to 45% ACN (VWR, 83639.320) in 10 mM ammonium formate (1ml formic acid (26N ...
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bioRxiv - Neuroscience 2021Quote: ... A virus or tracer was injected using a calibrated glass micropipette (VWR) at a rate of 0.3–0.5 μl/min ...
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bioRxiv - Immunology 2020Quote: ... sonicated for 45 seconds in an ultrasonic water bath (Branson 1210, VWR, Radnor, PA), and diluted to 2×105 PFU/ml (as determined in MDCK plaque assay ...
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bioRxiv - Biochemistry 2022Quote: ... Virus-containing supernatants were filtered (0.45 μm pore size cellulose-acetate filter (VWR)) ...
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bioRxiv - Microbiology 2020Quote: ... using a 50 kDa molecular weight cut off membrane (Spectra, VWR) and afterwards freeze dried.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were transfected using linear 25 kDa polyethylenimine (PEI) (VWR; AAA43896) at a 1:3 ratio between total μg of DNA plasmid (2.5 μg ...
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bioRxiv - Microbiology 2024Quote: ... Filtration was then performed through 10 kDa MWCO spin filter (VWR), at 14 000 × g for 15 min at 8°C and the samples were transferred into high-performance liquid chromatography vials for further analysis.
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bioRxiv - Neuroscience 2020Quote: ... rabies virus was delivered by pressure injection (Nanoject II Variable Volume Automatic Injector, VWR) in 23 nl increments over a 3 min and 10 sec interval to a final volume of 500 nl ...
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bioRxiv - Developmental Biology 2020Quote: ... The pooled virus-containing supernatant was filtered through a 0.45 μM PES membrane (VWR). The filtered supernatant was then spun in an ultracentrifuge for 2 hours at 20,000 g at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... The virus band was extracted and desalted on a NAP column (VWR, Cytiva, Illustra NAP) by elution in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Samples were loaded onto 10 kDa MWCO 500 μl centrifugal filters (VWR International) by combining 60 μl of lysate with 400 μl of Urea solution (8 M urea in 0.1 M Tris/HCl pH 8.5 ...
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bioRxiv - Plant Biology 2022Quote: ... Samples were loaded onto 10 kDa MWCO 500 μl centrifugal filters (VWR International) by combining 60 μl of lysate with 400 μl of Urea solution (8 M urea in 0.1 M Tris/HCl pH 8.5 ...
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bioRxiv - Microbiology 2024Quote: ... loaded the sample onto a 10 kDa 500 µL filter unit (VWR International) and centrifuged at 14,000 x g for 30 minutes ...
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bioRxiv - Systems Biology 2020Quote: ... 4°C) and the soluble fraction was collected and protein precipitated using ice-cold acetone (25% final concentration, VWR, USA) for 1h at - 20°C followed by centrifugation (400x g ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Cells expressing opsin-membrane proteins were incubated in the dark during 1h at 4°C on a nutating mixer (VWR) to increase active rhodopsin complexes ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stored at 4°C while the incubator cooled to 18°C and then protein expression was induced with 1 mM IPTG (VWR) and expressed at 18°C overnight ...
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bioRxiv - Immunology 2023Quote: ... Neutral pH fractionation was performed using a 60 min gradient of 4.5 to 45% ACN (VWR, 83639.320) in 10 mM ammonium formate (1ml formic acid (26N ...
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bioRxiv - Bioengineering 2023Quote: ... the virus-particle-containing medium was collected and filtered using a 0.45 µm syringe filter (VWR), and then centrifuged at 25,000 × g for 90 min at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... EV suspensions were concentrated on 3 kDa polyethersulfone (PES) membrane centrifugal filters (VWR International) for 5 min at 14,000 rpm at 4°C (Sigma 3-KIS centrifuge) ...
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bioRxiv - Microbiology 2020Quote: ... and centrifuged for 15 min through a 10 kDa cutoff microfuge column (VWR Scientific). For LC-MS/MS method #2 described in detail below ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared supernatants were concentrated using 3 kDa cut-off concentrator tubes (#516-0227P, VWR), according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... sonicated at 60°C for 30 min using a USC100TH sonicator (VWR, Vienna, Austria, 60 W, 45 kHz), and stored at -20°C ...
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bioRxiv - Synthetic Biology 2019Quote: ... the supernatant (containing secreted AAV virus) was collected and filtered through a 0.45-μm syringe filter (VWR). AAV virus was aliquoted into sterile Eppendorf tubes (0.5 mL/tube) ...
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bioRxiv - Cell Biology 2021Quote: ... P1 virus was propagated to passage 2 (P2) by infecting 50 ml of SF9 (VWR, #EM71104-3) culture and harvesting after 5–7 days as described above ...
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bioRxiv - Microbiology 2022Quote: ... Virus titers were quantified and titrated by real-time PCR (QuantaBio ToughMix®, VWR International, Radner, PA) with a TCID50/ml equivalent as previously described (34) ...
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bioRxiv - Biophysics 2020Quote: ... 50% 50mM Am-BIC buffer (pH 7.4) and shaken for 45 minutes at 900 rpm (VWR Thermal Shake Touch). 750 µL of ACN was added to each tube for 30 minutes to dehydrate the gel slices ...
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bioRxiv - Immunology 2021Quote: ... They were immersed in 4 mL of 4% paraformaldehyde (VWR), incubated at RT for 1 hour and finally incubated ON at 4°C to achieve full fixation ...
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bioRxiv - Bioengineering 2021Quote: ... The solution was then transferred into 12-14 kDa MWCO dialysis membranes (VWR, Chicago, IL, USA) and dialyzed against DI water for one week ...
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bioRxiv - Cell Biology 2020Quote: ... This step was repeated twice and the supernatant collected were vacuum centrifuged at 30°C for 45 min Samples were then reconstituted in 64 µL of 200 mM ammonium acetate (made from; ammonium Acetate, 98%; VWR) and transferred to amber glass UPLC vials (Waters) ...
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bioRxiv - Biochemistry 2019Quote: HDL samples prepared by adding 15 μL 3 mg/mL spin-labeled apoA-I probe to 45 μL 1 mg/mL HDL and drawn into an EPR-compatible borosilicate capillary tube (VWR) (32) ...
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bioRxiv - Pathology 2021Quote: ... 10% glacial acetic acid) at a 20:1 ratio for 30-45 min and 10% neutral buffered formalin (VWR scientific) at a 10:1 ratio for 24 h ...
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bioRxiv - Developmental Biology 2021Quote: Paraffin embedded brain sections were deparaffinized for 45 min at 60°C in the oven and 2x 10 min in Xylene (VWR). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... the enriched PBMCs at the middle layer were carefully removed and washed twice in 1% BSA (VWR, 9048- 45-8) in DMEM (Corning ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: All rats were trained to press the active lever for 45-mg banana-flavoured sucrose pellets (product # 76285-260, VWR) during daily ...
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bioRxiv - Biochemistry 2023Quote: ... were obtained from MilliporeSigma. Tris(2-carboxyethyl)phosphine (TCEP) hydrochloride (Cat. # K831, CAS: 51805-45-9) was purchased from VWR. Tris[(1-benzyl-1H-1,2,3-triazol-4- yl)methyl]amine (TBTA ...
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bioRxiv - Physiology 2021Quote: Paraformaldehyde (4%; VWR) fixed platelets were centrifuged onto poly-L-lysine coverslips (VWR ...
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bioRxiv - Cell Biology 2022Quote: ... once and fixed using 4% formaldehyde (Thermo Fisher)/4% sucrose (VWR) in 1x PBS for 15 minutes at room temperature ...