Labshake search
Citations for VWR :
1 - 33 of 33 citations for Collagen Binding Fragment since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... high concentration rat-tail collagen I (VWR) was neutralized with a 1:1 ratio of 100 mM HEPES (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... Appropriate number of acinar cells were then resuspended in premade collagen solution (1 mg/ml rat tail type1 collagen (VWR, Cat. No. CACB354236), 10× Waymouth’s media (Cedarlane Labs ...
-
bioRxiv - Bioengineering 2023Quote: Collagen I was isolated from frozen rat tails (VWR RLRBT297) based on a previously established method26 ...
-
bioRxiv - Neuroscience 2019Quote: ... cells were plated on collagen I coated plates (VWR, 62405-603) to promote cell attachment ...
-
bioRxiv - Bioengineering 2021Quote: ... Microchannels were coated with a rat tail collagen type I (VWR) solution of 0.1 mg/ml in DPBS (rat tail collagen I ...
-
bioRxiv - Cell Biology 2019Quote: ... 0.45μm low protein binding membrane (#28145-479) was from VWR International ...
-
bioRxiv - Neuroscience 2023Quote: ... Antibody binding was visualized with DAB-nickel (VWR, 101098-434). Sections were counterstained with nuclear fast red ...
-
bioRxiv - Bioengineering 2021Quote: ... Vascular compartments were coated with a rat tail collagen type I (VWR) solution of 0.1 mg/ml in Dulbecco’s phosphate-buffered saline (DPBS ...
-
bioRxiv - Physiology 2020Quote: ... Collagen in bone was stained with light green for 20min (720-0335, VWR) and fixed into acetic acid 1%.
-
bioRxiv - Bioengineering 2022Quote: ... Collagen solution was loaded into a 1mL syringe (10148-330, VWR, PA, USA) and the syringe was gently tapped to remove air bubbles ...
-
bioRxiv - Molecular Biology 2023Quote: Myoblasts expanded in growth media were seeded onto collagen-coated glass coverslips (VWR) in 6-well plates ...
-
bioRxiv - Immunology 2023Quote: ... they were dissociated and seeded on collagen-coated 6.5 mm transwells (Corning #29442-082, VWR). Transwells were pre-coated with 50µg/mL Collagen type I from Rat tail (BD Biosciences #354236 ...
-
bioRxiv - Cancer Biology 2022Quote: ... medium was replaced by 60 μL of 5 mg/mL Collagen Type I (VWR, 734-1085) in 1M NaOH and 1X DMEM/F12 ...
-
bioRxiv - Bioengineering 2023Quote: ... The prepared collagen solution was then transferred onto glutaraldehyde-coated coverslips (13 mm in diameter; VWR, Leuven, Germany) and placed under standard cell culture conditions of 37°C ...
-
bioRxiv - Neuroscience 2022Quote: ... then incubating in 5% normal horse serum for 60 min to block nonspecific binding (VWR, 102643-676). Afterwards ...
-
bioRxiv - Cancer Biology 2020Quote: ... Successively the endogenous peroxidases and non-specific binding sites were blocked using 3% H2O2 (VWR chemicals, 23615.248) and Protein-Block solution (DAKO Agilent technologies ...
-
bioRxiv - Cell Biology 2020Quote: ... the medium was removed and filtered through a 0.45-µm low-protein binding membrane (VWR International, Radnor, PA). McArdle-RH7777 or IMR-90 were then transduced with the virus with 8 µg/ml polybrene (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2021Quote: Human IgM antibodies were detected in sera from passively transferred mice using high-binding 384-well plates (VWR) coated with 0.5 µg/well of goat anti-human Ig polyvalent antibody (ThermoFisher ...
-
bioRxiv - Cell Biology 2019Quote: ... 50 µL of the collagen-I mixture was pipetted into the bottom of the wells of an 8-chamber slides (VWR) to coat the surface and allowed to gel for 20 minutes at 37°C ...
-
bioRxiv - Microbiology 2023Quote: Once primary airway epithelial cells reached ∼80% confluence, they were cultured on collagen coated cell culture inserts (Transwell®, 24-Well, 0.4 μm PET clear) (VWR) as previously published (Moreni et al. ...
-
bioRxiv - Cancer Biology 2024Quote: ... culture medium was changed and lentivirus-containing supernatant was collected 48 hours after transfection and filtered through a 0.45 μm low-protein-binding filter (VWR). Target cells were plated in 6-well culture plates with a 60-80% confluency ...
-
bioRxiv - Cell Biology 2020Quote: ... After 1h binding on a wheel at 4°C beads were washed 3x in PBS supplemented with 0.02% Tween20 (VWR, M147) and 1% SDS and eluted with NuPAGE LDS buffer supplemented with 50 mM DTT and 1 mM biotin at 95 °C for 10 min ...
-
bioRxiv - Genetics 2022Quote: ... A nonspecific antibody binding was prevented by an incubation of the fixed embryos in 3% BSA (VWR Life Science, 422351S) in PBS-T (1h ...
-
bioRxiv - Molecular Biology 2020Quote: ... Okazaki fragments were enriched by sequential elutions from Source15Q ion exchange columns (VWR 89128-854). Up to 1μg of fragments were ligated to adaptor primer pairs overnight at 16° and cleaned (GeneJET PCR Purification ...
-
bioRxiv - Pathology 2019Quote: ... total iron binding capacity (TIBC) and transferrin saturation were measured using the Stanbio Iron & TIBC kit (VWR, cat. number 10152-550). Serum ferritin was quantified using the mouse ferritin ELISA kit (Abcam ...
-
bioRxiv - Cancer Biology 2023Quote: ... Antibody binding was visualized using the using BrightVision Goat anti Rabbit / Poly-AP and BrightVision Goat anti Mouse / Poly-HRP (VWR/ImmunoLogic), with DAB as chromogen (Sigma-Aldrich) ...
-
bioRxiv - Cancer Biology 2021Quote: ... subcutaneous tumor fragments weighing 5–15 mg were homogenized using Kontes tubes with disposable pestles (VWR) in ice-cold 80:20 methanol:water (v/v) ...
-
bioRxiv - Systems Biology 2023Quote: ... Enzyme solutions for +M and -M conditions were prepared in separate 8-tube PCR strips (VWR #732-1521 or low-protein binding) by mixing 25 μL reaction buffer (+M/-M ...
-
bioRxiv - Pathology 2019Quote: ... The T1 fragment was placed into a VWR Personal Low Temperature Incubator (VWR Catalog number 89511-416) with varying temperature profiles depending on condition ...
-
bioRxiv - Cell Biology 2019Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
-
bioRxiv - Plant Biology 2019Quote: ... DNA fragments were separated in 2% (w/v) agarose gels and purified using the peqGOLD Gel Extraction Kit (VWR). Heat-shock transformation of chemically competent Escherichia coli DH5α cells were performed for all vectors followed by selection on LB-agar plates with ampicillin as a selection marker ...
-
bioRxiv - Molecular Biology 2020Quote: ... Purification of Okazaki fragments was accomplished by running the denatured genomic DNA through 400 ul Source 15Q (VWR 89128-854), binding at 300 mM NaCl ...
-
bioRxiv - Microbiology 2023Quote: ... salinarum were generated by isothermal Gibson assembly (Gibson, 2011) of: 1) the PCR fragments (amplified by KAPA Biosystems DNA polymerase [VWR] and gel extracted); and 2 ...