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Citations for VWR :
1 - 50 of 322 citations for Chikungunya Virus VLP E1 E2 C protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... coli E1 strain was grown overnight to stationary phase in LB Lenox Broth (VWR) before cells were washed 2x with PBS and OD600 determined by NanoDrop 2000c (Thermo Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stored at 4°C while the incubator cooled to 18°C and then protein expression was induced with 1 mM IPTG (VWR) and expressed at 18°C overnight ...
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bioRxiv - Microbiology 2021Quote: ... the virus suspension was mixed 1:1 with peqGOLD TriFast (VWR). Following ...
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bioRxiv - Neuroscience 2021Quote: ... A virus or tracer was injected using a calibrated glass micropipette (VWR) at a rate of 0.3–0.5 μl/min ...
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bioRxiv - Biochemistry 2022Quote: ... Virus-containing supernatants were filtered (0.45 μm pore size cellulose-acetate filter (VWR)) ...
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bioRxiv - Neuroscience 2020Quote: ... rabies virus was delivered by pressure injection (Nanoject II Variable Volume Automatic Injector, VWR) in 23 nl increments over a 3 min and 10 sec interval to a final volume of 500 nl ...
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bioRxiv - Developmental Biology 2020Quote: ... The pooled virus-containing supernatant was filtered through a 0.45 μM PES membrane (VWR). The filtered supernatant was then spun in an ultracentrifuge for 2 hours at 20,000 g at 4 °C ...
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bioRxiv - Systems Biology 2020Quote: ... 4°C) and the soluble fraction was collected and protein precipitated using ice-cold acetone (25% final concentration, VWR, USA) for 1h at - 20°C followed by centrifugation (400x g ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Cells expressing opsin-membrane proteins were incubated in the dark during 1h at 4°C on a nutating mixer (VWR) to increase active rhodopsin complexes ...
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bioRxiv - Plant Biology 2023Quote: ... was added to deactivate the protein (45°C, 4 hours) and the DNA was purified through phase lock gel (VWR) and precipitated with 1/10 volume of 3 M Sodium Acetate (Invitrogen) ...
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bioRxiv - Physiology 2020Quote: ... Magnetic Drive Centrifugal Pump (300W/600W/950W @ 0°C/10°C/20°C; VWR) and a 400W aquarium heater were used to maintain the temperatures in the cold and warm bath ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Magnetic Drive Centrifugal Pump (300W/600W/950W @ 0°C/10°C/20°C; VWR) and 2×400W aquarium heaters were used to maintain the temperatures in the cold and warm bath ...
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bioRxiv - Microbiology 2023Quote: ... The virus band was extracted and desalted on a NAP column (VWR, Cytiva, Illustra NAP) by elution in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... the virus-particle-containing medium was collected and filtered using a 0.45 µm syringe filter (VWR), and then centrifuged at 25,000 × g for 90 min at 4°C ...
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bioRxiv - Synthetic Biology 2019Quote: ... the supernatant (containing secreted AAV virus) was collected and filtered through a 0.45-μm syringe filter (VWR). AAV virus was aliquoted into sterile Eppendorf tubes (0.5 mL/tube) ...
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bioRxiv - Cell Biology 2021Quote: ... P1 virus was propagated to passage 2 (P2) by infecting 50 ml of SF9 (VWR, #EM71104-3) culture and harvesting after 5–7 days as described above ...
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bioRxiv - Microbiology 2022Quote: ... Virus titers were quantified and titrated by real-time PCR (QuantaBio ToughMix®, VWR International, Radner, PA) with a TCID50/ml equivalent as previously described (34) ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
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bioRxiv - Cell Biology 2020Quote: ... virus-containing supernatant was collected 48 h later and filtered through a 0.45 μm polyethersulfone filter (VWR 28145-505). For protein-coding constructs ...
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bioRxiv - Microbiology 2023Quote: ... The virus containing band was extracted from the gradient and desalted on a NAP column (VWR, Cytiva, Illustra NAP) by elution in PBS ...
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bioRxiv - Genomics 2022Quote: ... The supernatant (which contains the majority of virus) was filtered through a 0.2 μM PES filter (VWR, cat. #97066-214). 25 mL of 40% PEG8000 (JT Baker ...
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bioRxiv - Immunology 2019Quote: ... Cell monolayers were processed for ICC staining at 24 to 72 hpi depending on virus replication kinetics (as previously described [94]) or stained with Giemsa stain (VWR; 35086). Relative plaque titre was calculated as the plaque titre of a virus stock under the indicated condition divided by its titre under a control condition ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
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bioRxiv - Microbiology 2020Quote: ... monocytogenes strains were grown at 37°C or 30°C in brain heart infusion (BHI) medium (237500; VWR) or minimal medium supplemented with glucose as the sole carbon source ...
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bioRxiv - Genomics 2022Quote: ... with a traceable digital thermometer (Accuracy: ±0.05°C, resolution: 0.001°, temperature range: –50 to 150°C, VWR, USA), and salinity with a traceable digital portable conductivity meter (Accuracy ...
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bioRxiv - Microbiology 2023Quote: ... monocytogenes strains were grown at 37°C or 30°C in brain heart infusion (BHI) medium (237500; VWR) or defined medium supplemented with glucose as the sole carbon source ...
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bioRxiv - Cancer Biology 2020Quote: ... at 56°C (VWR Seradigm, Lot 120B14). All cells were cultured in a Heracell (Thermofisher ...
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bioRxiv - Cell Biology 2023Quote: ... + 0.5% BSA-c (Aurion, VWR, Cat# 25557) + 2% fish skin gelatin (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... Protein bands were visualized using Blue BANDitTM protein stain (VWR Life Science, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227), and incubated at 100L°C for 10Lmin under reducing conditions ...
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bioRxiv - Genetics 2022Quote: ... Pre-denaturation was performed at 91°C for 3 min and then 60°C for 20 min on heat blocks (VWR). After plates were spun ...
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bioRxiv - Genetics 2022Quote: ... Predenaturation was performed at 91 °C for 3 minutes and then 60 °C for 20 minutes on heat blocks (VWR). After plates were spun ...
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bioRxiv - Neuroscience 2019Quote: ... 100 °C on block heater (VWR, Radnor, PA). 25ug of protein was loaded into homemade 4-8% gradient polyacrylamide gels ...
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bioRxiv - Microbiology 2022Quote: ... 1 volume of cold (4°C) TCA (VWR) was mixed with 4 volumes of supernatant and incubated on ice for 60 minutes ...
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bioRxiv - Physiology 2021Quote: ... incubated at 37°C for 120 min and inactivated at 85°C for 5 min in a thermal cycler (VWR UNO 96). cDNA was then stored at −20°C until further use ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were counted using C-chip disposable hemocytometer (VWR, International Ltd ...
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bioRxiv - Cell Biology 2022Quote: ... Buffer C consisted of 0.1% phosphoric acid (85%, VWR) in 60% acetonitrile (acidic wash ...
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bioRxiv - Cell Biology 2023Quote: ... and a 2x volume −20°C ethanol (VWR Chemicals). After overnight incubation at −20°C the DNA was pelleted at 17000g for 30 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 4 mL of 80% Methanol (-20°C, VWR, #85800290) was directly added to cells with the flasks on ice ...
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bioRxiv - Neuroscience 2022Quote: ... with chamber temperature of 21 ± 3°C and object temperature of 19±3°C and sections were mounted directly on Superfrost glass (631-0108, VWR, Leuven, The Netherlands) in series of 10 per each brain and stored at −80°C.
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bioRxiv - Biophysics 2023Quote: ... The concentration of total proteins was determined with the BCA Protein Assay Kit (VWR International GmbH, Darmstadt, Germany). According to the total protein amount in each sample ...
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bioRxiv - Bioengineering 2023Quote: ... and dried on a 50 °C hot plate (VWR, Germany) for 5 min in the fume hood ...
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bioRxiv - Neuroscience 2023Quote: ... at -20°C and mounted on Superfrost® slides (VWR) and allowed to dry for two hours before storage in - 80°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein was estimated using Bradford reagent (VWR).
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred to BiotraceNT membrane (VWR) and incubated with primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... and peqGOLD Protein-Marker I Unstained (VWR) were used as protein markers ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant protein extracts were recovered by centrifugation and protein concentration in samples was determined using Bradford Reagent (VWR).
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bioRxiv - Microbiology 2020Quote: ... Each fraction was resuspended in HEPES-NaCl buffer and 10 μL of each fraction was examined for protein concentration and lipid content using a Bradford protein assay (VWR) and the lipophilic FM4-64 dye (Molecular Probes) ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated at 37° C on an orbital shaker (VWR). Single strain controls were grown in parallel to ensure growth and Dox sensitivity was similar in 96 well plates compared to test tubes ...