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Citations for VWR :
1 - 50 of 52 citations for African Swine Fever Virus P30 E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... gentamycin (10 μg/ml for plasmid maintenance in E. coli; 100 μg/ml for plasmid maintenance in P. aeruginosa, VWR, Cat. No. 97062-974).
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bioRxiv - Microbiology 2022Quote: ... African green monkey kidney cells (Vero) were maintained in DMEM supplemented with 5% heat-inactivated fetal bovine essence (FBE) (VWR Life Sciences 10803-034), 1% Penicillin/Streptomycin (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... H&E routine stain was performed using Leica ST5010 Autostainer XL and Leica ST Infinity H&E Staining System kit (VWR#3801698) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the virus suspension was mixed 1:1 with peqGOLD TriFast (VWR). Following ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21(DE3) (Stratagene Europe/VWR, Darmstadt, Germany). Protein expression was induced with 0.5 mM isopropyl β-d-thiogalactopyranoside for 18 h at 20 °C ...
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bioRxiv - Neuroscience 2021Quote: ... A virus or tracer was injected using a calibrated glass micropipette (VWR) at a rate of 0.3–0.5 μl/min ...
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bioRxiv - Cell Biology 2022Quote: ... and digested with 1 mg/mL pronase E (39052, VWR) per 1 g tissue for 6-8 h at 37 °C and 5% CO2 under constant agitation ...
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bioRxiv - Biochemistry 2022Quote: ... Virus-containing supernatants were filtered (0.45 μm pore size cellulose-acetate filter (VWR)) ...
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bioRxiv - Microbiology 2023Quote: ... coli strains were grown on Luria–Bertani agar (VWR) or in Luria–Bertani broth (VWR ...
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bioRxiv - Neuroscience 2020Quote: ... rabies virus was delivered by pressure injection (Nanoject II Variable Volume Automatic Injector, VWR) in 23 nl increments over a 3 min and 10 sec interval to a final volume of 500 nl ...
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bioRxiv - Developmental Biology 2020Quote: ... The pooled virus-containing supernatant was filtered through a 0.45 μM PES membrane (VWR). The filtered supernatant was then spun in an ultracentrifuge for 2 hours at 20,000 g at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... coli DH5α was grown in Luria Bertani (LB) broth (VWR), and E ...
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bioRxiv - Bioengineering 2019Quote: ... coli loaded microbeads were cultures in Lysogeny broth (LB; VWR). For the generation of degradable alginate microbeads ...
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bioRxiv - Microbiology 2021Quote: ... coli Rossetta2 (DE3) was grown in Terrific Broth (TB, VWR) for protein purification ...
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bioRxiv - Microbiology 2023Quote: ... The virus band was extracted and desalted on a NAP column (VWR, Cytiva, Illustra NAP) by elution in PBS ...
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bioRxiv - Microbiology 2019Quote: ... coli BL21 (DE3) was grown in Terrific broth (VWR, PA, USA) for protein purification ...
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bioRxiv - Microbiology 2024Quote: ... Escherichia coli DH5α strains were grown in Luria-Bertani Broth (VWR) at 37°C and supplemented with 200 μg/mL hygromycin and 50 μg/mL kanamycin ...
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bioRxiv - Bioengineering 2023Quote: ... the virus-particle-containing medium was collected and filtered using a 0.45 µm syringe filter (VWR), and then centrifuged at 25,000 × g for 90 min at 4°C ...
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bioRxiv - Genomics 2024Quote: ... 5 mM Na3 citrate 2H2O) with ∼75u/mL Pronase E (VWR, Radnor, PA) was added to each sample ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Tygon tubing was replaced by flexible pumping tubing (Masterflex Tygon E-3603, VWR). Outflow tubing were connected to outlet ports ...
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bioRxiv - Bioengineering 2020Quote: ... coli strains were cultured in lysogeny broth (LB) (VWR International, Randor, PA) at 37 °C with rotary shaking at 250 rpm ...
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bioRxiv - Microbiology 2019Quote: ... coli DH5α was grown in Luria Bertani (LB) broth (VWR, PA, USA), and E ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21 Star (DE3) or OverExpress C43 (DE3) (Lucigen/VWR, Radnor, PA). Following induction with IPTG ...
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bioRxiv - Microbiology 2022Quote: ... coli DH5α was propagated in Luria Bertani (LB) broth (VWR, PA, USA), and E ...
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bioRxiv - Microbiology 2023Quote: ... coli were grown overnight to stationary phase in LB Lenox Broth (VWR) before cells were washed 2x with PBS and OD600 determined by NanoDrop 2000c (Thermo Scientific) ...
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bioRxiv - Synthetic Biology 2019Quote: ... the supernatant (containing secreted AAV virus) was collected and filtered through a 0.45-μm syringe filter (VWR). AAV virus was aliquoted into sterile Eppendorf tubes (0.5 mL/tube) ...
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bioRxiv - Cell Biology 2021Quote: ... P1 virus was propagated to passage 2 (P2) by infecting 50 ml of SF9 (VWR, #EM71104-3) culture and harvesting after 5–7 days as described above ...
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bioRxiv - Microbiology 2022Quote: ... Virus titers were quantified and titrated by real-time PCR (QuantaBio ToughMix®, VWR International, Radner, PA) with a TCID50/ml equivalent as previously described (34) ...
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bioRxiv - Cell Biology 2022Quote: ... coli DB (DBS0350636) in a type 25384-302 petri dish (VWR, Radnor, PA). 100 μg/ml dihydrostreptomycin and 100 μg/ml ampicillin were used to kill E ...
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bioRxiv - Microbiology 2022Quote: ... coli BL21 (DE3) was propagated in Terrific broth (TB) medium (VWR, PA, USA) for protein purification.
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bioRxiv - Cell Biology 2023Quote: ... coli DB (DBS0350636) in a type 2538-4302 petri dish (VWR, Radnor, PA). 100 μg/ml dihydrostreptomycin and 100 μg/ml ampicillin were used to kill E ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
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bioRxiv - Biophysics 2019Quote: ... The vial with the cell suspension was connected via Tygon S3 E-3603 tubing (VWR, inner diameter 1/32" ...
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bioRxiv - Bioengineering 2020Quote: All Escherichia coli strains were cultured in lysogeny broth (LB) (VWR International, Randor, PA) at 37 °C with rotary shaking at 250 rpm ...
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bioRxiv - Microbiology 2023Quote: ... coli E1 strain was grown overnight to stationary phase in LB Lenox Broth (VWR) before cells were washed 2x with PBS and OD600 determined by NanoDrop 2000c (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... coli expression constructs were grown overnight shaking at 37 °C in Terrific Broth (VWR) supplemented with 5 mM MgSO4 and kanamycin (50μg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... virus-containing supernatant was collected 48 h later and filtered through a 0.45 μm polyethersulfone filter (VWR 28145-505). For protein-coding constructs ...
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bioRxiv - Microbiology 2023Quote: ... The virus containing band was extracted from the gradient and desalted on a NAP column (VWR, Cytiva, Illustra NAP) by elution in PBS ...
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bioRxiv - Microbiology 2023Quote: ... then treated with Pronase E (100µg/ml in Tris-HCl (pH 7.2) + 0.06% NaCl) (VWR Chemicals) for 2 hours at 60C to remove proteins covalently bound to PG ...
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bioRxiv - Genomics 2022Quote: ... The supernatant (which contains the majority of virus) was filtered through a 0.2 μM PES filter (VWR, cat. #97066-214). 25 mL of 40% PEG8000 (JT Baker ...
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bioRxiv - Immunology 2019Quote: ... Cell monolayers were processed for ICC staining at 24 to 72 hpi depending on virus replication kinetics (as previously described [94]) or stained with Giemsa stain (VWR; 35086). Relative plaque titre was calculated as the plaque titre of a virus stock under the indicated condition divided by its titre under a control condition ...
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bioRxiv - Plant Biology 2023Quote: ... coli DH5α and positive clones were selected via Colony PCR using Taq polymerase (VWR, Radnor, USA). Correct insertion was checked via Sanger sequencing at Eurofins Genomics (Ebersberg ...
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bioRxiv - Microbiology 2023Quote: ... coli cells were grown at 37 °C with aeration in either LB (Luria-Bertani broth; VWR) or LA (Luria Agar ...
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bioRxiv - Cell Biology 2023Quote: ... coli Gold bacteria were cultured in Luria-Bertani (LB) medium (Broth, Miller, VWR, Radnor, PA, USA) supplemented with 150 μg/ml ampicillin for 4.5 and 6 hours ...
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bioRxiv - Biochemistry 2022Quote: ... and were treated for 2hrs at 60°C with activated Pronase E (100µg/ml final concentration, pre-treated for 2hrs at 60°C in the digestion buffer; supplier: VWR). The sacculi were incubated at 100°C for 10min to inactivate the Pronase E and were washed 3 times with 1ml of MQ water ...
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bioRxiv - Biochemistry 2022Quote: ... and were treated for 2hrs at 60°C with Pronase E (100µg/ml final concentration, pre-treated for 2hrs at 60C in the digestion buffer; supplier: VWR). The sacculi were incubated at 100°C for 10min to inactivate the Pronase E ...
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bioRxiv - Cell Biology 2019Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
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bioRxiv - Microbiology 2023Quote: ... coli (E23) CRISPRi cell lines (details below) were grown overnight to stationary phase in LB Lenox Broth (VWR) before dilution 1:50 into fresh media in the morning ± 2 µM anhydrotetracycline ...
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bioRxiv - Biophysics 2021Quote: ... The sandwich consists of a bottom layer of PAA gel (E ≈ 1800 Pa) and a top layer of a 12 mm round glass cover slip (VWR).
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bioRxiv - Developmental Biology 2023Quote: ... transgenic Hydra from strains Tg(actin1:GFP)rs3-in and Tg(tba1c:mNeonGreen)cj1-gt were dissociated using Pronase E (VWR, E629-1G) as previously described (Siebert et al. ...