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Citations for VWR :
1 - 50 of 1821 citations for 8 BROMO 4 METHYLTHIO 7 PHENYLPYRAZOLO 1 5 A 1 3 5 TRIAZIN 2 AMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... with 100 μg/mL 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal, VWR), or with 0.1 μM CdCl2 (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... animals were soaked in 20 mg/mL 5-bromo-2’-deoxyuridine (VWR) with 3% DMSO in a 6 g/L Instant Ocean salt solution for 2 hours followed by a 4-hour chase prior to processing using the rapid protocol ...
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bioRxiv - Microbiology 2019Quote: ... The membranes were washed 3 times in TBST for 15 minutes each and blots were developed with BCIP/NBT (5-bromo-4-chloro-3-indoyl-phosphate/nitro blue tetrazolium) color development substrate (VWR).
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bioRxiv - Cell Biology 2022Quote: ... were grown to O.D 0.3-0.6 and incubated with non-fluorescent Halo tag ligand 7-bromo-1-heptanol (7BRO, 100μM, VWR #AAH54762) for 10 min to irreversibly mask all the pre-existing ribosomal proteins ...
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bioRxiv - Microbiology 2021Quote: ... and 300μL of XTT solution was added to each well (0.5mg/mL XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] (VWR) with 25μM menadione in PBS) ...
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bioRxiv - Microbiology 2023Quote: ... 4 °C) and the cells were resuspended in 5 mL of methanol/dichlormethane/ethylacetate 10:2:3 (dichlormethane: SupraSolv, VWR Chemicals ...
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bioRxiv - Cell Biology 2023Quote: ... diluted 1:50 in 5 % BSA/TBST overnight at 4°C (BSA: VWR; #422361V). As a negative control ...
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bioRxiv - Microbiology 2023Quote: ... The cells were washed off the filter using 5 mL ice-cold methanol/ethanol/chloroform 1:3:1 (methanol: HiPerSolv Chromanorm, VWR Chemicals ...
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bioRxiv - Molecular Biology 2023Quote: ... then neutralized with 1 μL 5 M HCl (VWR BDH7419-1). The remainder of the library preparation was performed according to the xGen protocol except that post-ligation ...
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bioRxiv - Genomics 2022Quote: ... 30 μl of chloroform (1:5, VWR International) was added and the samples were incubated for further 3 min at RT before centrifuged at 13,000g ...
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bioRxiv - Bioengineering 2020Quote: ... 4 μL of NiSO4 (5 mM, VWR) was added for further 5-min incubation ...
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bioRxiv - Immunology 2020Quote: RNA was extracted using a modified phenol-chloroform protocol (Chomczynski and Sacchi, 2006) with 1-Bromo-3-chloropropane and Isopropanol (Sigma and VWR, respectively). Total RNA was quantified and assessed for quality and integrity by Bioanalyser (RNA Pico 6000 Chip ...
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bioRxiv - Synthetic Biology 2023Quote: ... then plated onto YPM supplemented with 60 µg mL-1 5-fluorocytosine (5-FC) (TCF0321, VWR). Colonies that emerged were then transferred onto a new YPM 5-FC agar plate for clonal isolation ...
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bioRxiv - Microbiology 2021Quote: ... 200µg/ml 5’-fluoro-2’-deoxyuridine (VWR), 100ng/ml anhydrotetracycline (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... 5-Flouro 2’Deoxyuridine was from VWR; 5-Methyluridine was from Alfa Aesar ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we thawed yeast RBD library and the Wuhan Hu-1 and Omicron BA.1 strains by inoculating the corresponding glycerol stocks in SDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% dextrose [VWR #90000– 904] ...
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bioRxiv - Cancer Biology 2023Quote: ... which were then blocked in a 5% BSA (VWR, 9048-46-8) solution made in 1x TBST buffer (20 mM Tris base ...
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bioRxiv - Biochemistry 2020Quote: ... HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (VWR, Radnor, PA), NaCl (VWR) ...
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bioRxiv - Genomics 2024Quote: ... and vortexed at speed 3 (∼600rpm) for 5 minutes (VWR Analog Vortex Mixer ...
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bioRxiv - Cell Biology 2023Quote: ... were dried to a 5 mm coverslip (Mentzel Glasser #1, VWR) and mounted on the sample holder using vacuum grease (Dow Corning high vacuum grease ...
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bioRxiv - Immunology 2023Quote: ... Gametes were washed with PBS and incubated with mAbs dilutions in PBS containing 0.5% PBS and 0.05% NaN3 (SIFA buffer) for 1 hr at 4 °C in sterile V bottom plates (VWR#736-0223). After incubation ...
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bioRxiv - Biochemistry 2023Quote: ... with 5% 2-mercaptoethanol (VWR Life Science #M131-100ml) and then ran on 4-20% Criterion TGX pre-cast gels (Bio-Rad #5671093) ...
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bioRxiv - Neuroscience 2022Quote: ... incubated for 5 minutes with fluorescent nuclear DAPI stain (VWR; 1:5000), and mounted with DAKO immunofluorescence mounting media (Life Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... incubated for 5 minutes with fluorescent nuclear DAPI stain (VWR; 1:10000), and mounted with Slow fade gold antifade reagent (Life Technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... The samples were washed three times with PBST and then incubated with 4′,6-diamidino-2-phenylindole (DAPI, 5 µg/mL in PBS) (VWR) and phalloidin (1.5 µM in DMSO ...
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bioRxiv - Developmental Biology 2021Quote: ... 4 ml of pre-chilled (−20°C) methanol/acetic acid (3:1, VWR chemicals) was added dropwise and flicking to homogenize ...
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bioRxiv - Biophysics 2021Quote: ... the coverslips/slides were placed in a 1% aminosilane solution (94 mL methanol, 1 mL 3-(2-aminoethylamino)propyltrimethoxysilane (VWR, CAS# 1760-24-3), 5 mL glacial acetic acid ...
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bioRxiv - Biochemistry 2024Quote: ... Fiberlite F21-8×50y rotor) and applied to a 5 ml HisTrap Column (VWR, Cytiva) mounted on an Aekta Purifier 100 FPLC system.
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bioRxiv - Biochemistry 2020Quote: ... samples were washed for 3×5 minutes in PBSM and permeabilized for 10 minutes in PBSM + 5 mg/mL BSA (VWR VWRV0332-25G) + 0.1% Triton-X100 (Sigma-Aldrich T8787-100mL) ...
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bioRxiv - Biophysics 2020Quote: VLPs suspended in PBS buffer were mixed at 4°C with KIF5B-spycatcher in (buffer) for 7-8 hours on a nutating mixer (VWR, Radnor, PA) to ensure Spy Tag-Spy Catcher binding ...
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bioRxiv - Molecular Biology 2023Quote: ... TGIRT was dissociated from the RNA-DNA duplexes by addition of 1 μL 5 M NaOH (VWR BDH7225-1) with incubation at 95 °C for 3 minutes ...
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bioRxiv - Microbiology 2023Quote: ... and the cells were resuspended in 5 mL of methanol/dichlormethane/ethylacetate 10:2:3 (dichlormethane: SupraSolv, VWR Chemicals, Dresden, Germany; ethylacetate, HiPerSolv Chromanorm, VWR Chemicals, Dresden, Germany). The suspensions were sonicated (5 min ...
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bioRxiv - Microbiology 2020Quote: ... were co-transfected into cells at a ratio of 3:2:1 using PEI (VWR). After 16 h incubation ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1% Tween20) and were fixed in egg buffer with 1% paraformaldehyde for 5 minutes on a Superfrost Plus slide (VWR). Gonads were then flash frozen in liquid N2 and the cover slip was removed ...
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bioRxiv - Synthetic Biology 2023Quote: ... ribosome was purified using hydrophobic chromatography (3×5 mL HiTrap Butyl HP column (VWR)) with Fast Performance Liquid Chromatography (FPLC ...
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bioRxiv - Molecular Biology 2019Quote: ... 5% non-fat milk and 1% BSA filtered through a paper filter (qualitative, 415, VWR). Blots were rinsed 1-2 times with Wash Buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... 10^5 human SHSY-5Y cells seeded on 18 mm #1 round cover glass (VWR) in a 12 well culture plate ...
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bioRxiv - Microbiology 2023Quote: ... 1 mL of swollen conidia was aliquoted into a 5 mL screwcap centrifuge tube (VWR). To each 1 mL aliquot ...
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bioRxiv - Microbiology 2021Quote: ... The grid was stained 3x with 5 μL of 2% uranyl acetate (VWR) with blotting immediately after application for the first two rounds of staining ...
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bioRxiv - Immunology 2021Quote: ... BSA 5% (VWR) and fluorescently labelled antibodies at proper concentration ...
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bioRxiv - Evolutionary Biology 2022Quote: ... EBY100 cells were thawed by inoculating 5 mL YPD (1% Bacto yeast extract (VWR #90000-726), 2% Bacto peptone (VWR #90000-368) ...
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bioRxiv - Cell Biology 2023Quote: ... cells grown in 2- or 4-well chamber slides (model 1 German borosilicate; Lab-Tek; VWR) were fixed with freshly prepared 4% formaldehyde in PBS solution (using 16% Methanol-free Formaldehyde ...
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Synaptonemal Complex dimerization regulates chromosome alignment and crossover patterning in meiosisbioRxiv - Cell Biology 2020Quote: ... pSUN-1::TIR-1::mRuby him-8(tm611) spo-11-aid IV (ROG212) adult hermaphrodites were grown on 1 mM auxin (indole-3-acetic acid, VWR AAA10556-36) and therefore lacked SPO-11 ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in PBS (3 x 5 min) and mounted on slides (Superfrost Plus, VWR) with mounting medium (Fluoromount ...
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bioRxiv - Cell Biology 2020Quote: ... with a 1/8” tapered microtip (VWR International, 33996-163). The coverslips were then moved immediately into unroofing buffer containing 2% glutaraldehyde (Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... 5 Prime heavy 2 mL-phase lock gels (#2302830) were from VWR (Radnor, PA). High Capacity cDNA Reverse Transcription Kit (#4368814 ...
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bioRxiv - Microbiology 2023Quote: ... every 2 days or a cocktail of PGEs inhibitor (CAY10526 5 mg/kg VWR) and prostanoid receptor antagonist 2 (AH6809 5mg/kg Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... 4-cell stage embryos were mounted on a thin (∼1-2x lab tape thickness) 2% agar pad on a glass slide (VWR VistaVision, 3 inches x 1 inch x 1 mm) using a hand-pulled glass pipette (VWR Pasteur Pipette ...
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bioRxiv - Genomics 2021Quote: ... 5% CO2 at a density between 0.3-1 x 106cells/mL in RPMI medium (VWR 45000-396) topped up with 10% Fetal Bovine Serum (Genesee Scientific ...
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bioRxiv - Microbiology 2022Quote: ... prepared as a 1:5 dilution in buffer (pH=7.2) made using Gurr buffered tablets (VWR #331942F) and filtered ...