Labshake search
Citations for VWR :
1101 - 1150 of 1181 citations for 7 ACETOXY 2 CHLORO 1 HEPTENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and 1 mL of supernatant was transferred to a borosilicate glass culture tube pre-cleaned with HPLC-grade methanol (VWR International). Then 1mL of EA was added to the remaining sample (to maximize extraction efficiency) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cells were then washed with 25 mL of ice cold STM buffer (pH = 7.5, 270 mM sucrose, Millipore Sigma, 1.07651.5000, 10 mM Tris HCl, Alfa Aesar, J67233, 1 mM MgCl2, VWR, E525-100ML) and spun down again at 4°C for 5 minutes at 1500 x g ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were visualised on a 1% agarose gel (run for 30 minutes at 135 V) with GelGreen (VWR, 730-1535) using an ImageQuant™ LAS 500 (GE Lifesciences) ...
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bioRxiv - Biophysics 2023Quote: ... and 1%(v/v) ammonium persulfate (APS) for 60 minutes at RT on plasma cleaned glass cover slips (No.1, 25mm Ø, VWR) that were pre-treated with 3-aminoproyltriethoxysiliane (APTES ...
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bioRxiv - Genetics 2023Quote: ... hTERT RPE-1 cells (ATCC, CRL-4000) were maintained in DMEM:F-12 media (ATCC, 30-2006) supplemented with 10% FBS (VWR, 97068-091) and 1% Pen-Strep (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... then incubated on rollers at room temperature for 1 hour with an anti-mouse secondary antibody conjugated to horseradish peroxidase (VWR, NXA931). After four further washes for 10 min each (2 x blocking buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... for 50 ms at 1 s intervals using electrodes (diameter 3 mm; Sonidel CUY650P3) and ECM-830 BTX square wave electroporator (VWR international). After electroporation ...
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bioRxiv - Microbiology 2024Quote: ... aureus strains were generated as previously described 101 and transformed with ∼5 µg of the pIMAY construct using the BioRad Gene Pulser Xcell electroporation system and 1 mm disposable electroporation cuvettes (VWR Signature). The pulse was 21kV/cm ...
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bioRxiv - Immunology 2024Quote: ... Gametes were washed with PBS and incubated with mAbs dilutions in PBS containing 0.5% PBS and 0.05% NaN3 (SIFA buffer) for 1 hr at 4 °C in sterile V bottom plates (VWR#736-0223). After incubation ...
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Synaptonemal Complex dimerization regulates chromosome alignment and crossover patterning in meiosisbioRxiv - Cell Biology 2020Quote: ... pSUN-1::TIR-1::mRuby him-8(tm611) spo-11-aid IV (ROG212) adult hermaphrodites were grown on 1 mM auxin (indole-3-acetic acid, VWR AAA10556-36) and therefore lacked SPO-11 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and was subsequently propagated in a well of an unshaken flat-bottom polypropylene 96-well plate in one of three conditions: YPD (1% Bacto yeast extract (VWR #90000–726), 2% Bacto peptone (VWR #90000–368) ...
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Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... we plated a 150 μL of 1:100 diluted saturated culture of a sensitive strain (YAN563) on a single-well (VWR #46600-638) methylene blue agar plate (20 g/L peptone ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated with chemiluminesence substrate (Perkin-Elmer: NEL105001EA) for 1 min and signal was developed on GE Amersham Hyperfilm ECL (VWR: 95017-661). Membranes were stripped by 2 x 7 min incubations in stripping buffer (6 M guanidine hydrochloride (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... The films were rinsed in dH2O and covered with large coverslips (80 × 120 mm, thickness no.1, VWR International AS, Oslo, Norway). Imaging was performed with an epi-fluorescence microscope (Olympus BX51) ...
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bioRxiv - Biophysics 2022Quote: ... the surface of the glass coverslip serving as chamber bottom was incubated with 1% (10 mg/ml) bovine serum albumin (BSA; VWR, Radnor, PA) in phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2019Quote: ... 2-3 × 105 single cells EpiSCs were seeded on 5 μg/10 cm2 fibronectin-coated 6-well plates in media containing (1 μl/ml) ROCK inhibitor (InSolution™ Y-276320, Cat.#688001-500UG, VWR Calbiochem). In the morning of transfection ...
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bioRxiv - Microbiology 2020Quote: ... each well was washed three times with warm PBS and then treated with 500ul PBS with 1% Triton X-100 (AMRESCO, VWR, Randor PA) for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: Polar metabolites were first extracted from the frozen SHIME-samples by means of ultra pure water (0,055 μS cm-1) obtained via a purified water system (VWR International, Merck, Germany). For this purpose ...
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bioRxiv - Microbiology 2021Quote: ... enterocolitica samples were eluted by washing the wells 3 times with warm PBS followed by treatment with 500 μl PBS with 1% Triton X-100 (AMRESCO, VWR, Randor, PA) for 5 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... and 1/5th of the lysate was added into 40 µL lysis buffer and sheared in a sonicator (VWR Aquasonic 250D, 35Khz) for 3 min ...
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bioRxiv - Molecular Biology 2022Quote: ... the medium was changed and the incubation continued for another 24 hours before addition of 1-5 µg/mL puromycin (VWR 97064-280). Incubation with puromycin continued for several days until untransfected control wells were depleted of cells ...
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bioRxiv - Neuroscience 2021Quote: ... After which cells were washed once with 1x PBS with 1% BSA before being filtered through 40um FlowMi Tip Strainers (VWR, 10032-802). Cells were then diluted with 1x PBS with 1% BSA and loaded onto the Chromium 10x Controller according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl of each reaction were mixed with loading dye and analyzed on a 1% (w/v) agarose gel containing GelRed (VWR, Darmstadt, Germany). The remaining PCR mix was purified using a QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... the tissue was first blocked for 30 min using a blocking buffer (TBS with 1% BSA (VWR, 22013, Bovine Serum Albumin (BSA), fraction V ...
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bioRxiv - Immunology 2021Quote: ... Whole blood cell lysates or lysates of PBMCs from IL-1-Ra-Ab positive patients were treated with alkaline phosphatase as previously described using FastAP thermosensitive alkaline phosphatase (Fermentas/VWR, Darmstadt, Germany).(40 ...
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bioRxiv - Microbiology 2019Quote: ... Reactions were stopped by incubating on ice for 5 min and digesting with 1 µg of Proteinase K Solution (VWR, PA, USA) for 15 min at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... both supplemented with 10 μg/mL chloramphenicol (Cm) and 10 μM (mild induction) or 100 μM (strong induction) of isopropyl β-D-1-thiogalactopyranoside (IPTG) (43714 5X, VWR Chemicals) when necessary.
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bioRxiv - Bioengineering 2020Quote: ... and particles were removed from PDMS chip and glass substrate (76 x 26 x 1 mm microscope slides, VWR International GmbH, Germany) via isopropanol washing and drying with pressurized air ...
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bioRxiv - Cancer Biology 2021Quote: ... was achieved using an adapted protocol from Ly et al.22 FFPE TMAs were first washed twice for 1 minute in a xylene bath (mixture of isomers; ≥98.5%, AnalaR NORMAPUR®, VWR, Leicestershire, UK). Residual xylene was removed ...
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bioRxiv - Neuroscience 2019Quote: ... the aggregates were washed 3× in a 15-ml tube by sedimentation in 3:1 medium and then maintained in suspension on sterile petri dishes (VWR, #391‒2016) in 10 – 15 ml 3:1 medium with media changes every other day ...
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bioRxiv - Microbiology 2019Quote: ... One single colony was picked and incubated for 8 to 10 h at 30°C in either 4.5 ml or 1 ml BHI containing 154mM acetate in reaction tubes or Deepwell-plates (VWR International, Pennsylvania, USA), respectively ...
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bioRxiv - Microbiology 2019Quote: ... 2 µL of 1:10 diluted purified anti-SpB antibody1 and 2µL of Cy3 conjugated Goat Anti Rabbit IgG Polyclonal Antibody (VWR R611-104-122) were added to 40 µL of 0.4 O.D ...
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bioRxiv - Immunology 2020Quote: One mL of BMDM from lipin-1mKO and littermate control mice at a concentration of 1×106/mL was placed into falcon tubes (VWR-60818-500). Cells were either treated with or without IL-4 (40ng/mL ...
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bioRxiv - Immunology 2020Quote: ... Unbound protein was washed away with PBST (1x PBS with 0.5% Tween) followed by blocking with 1% casein (w/v) in PBS (VWR International, Radnor, PA) for 2 h at room temperature with mild shaking (55 rpm) ...
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bioRxiv - Microbiology 2022Quote: ... 1 cell line was cultured at 37°C with 5% CO2 in DMEM with high glucose and L-glutamine (VWR 16777-129) supplemented with 10% heat inactivated FBS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The reaction mixture was incubated at room temperature for 60 mins before quenched by adding 12 µL 1% formic acid (VWR, Radnor, PA).
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bioRxiv - Bioengineering 2024Quote: ... AB_11125142) or an anti-mouse secondary antibody (1:3000, Cat# 170-6516, RRID: AB_11125547) and enhanced chemiluminescence reagent (VWR, Cat # 490005-018). The signal was visualized using a C-DiGit Blot Scanner (Li-Cor) ...
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bioRxiv - Cell Biology 2023Quote: ... washed cells were suspended at a concentration of 1 × 106 cells/mL in the growth medium and filtered using a 40 µm cell strainer (VWR: 10199- 655). Cells were then sorted into 15 mL collection tubes using a sterile 100 µm chip (Sony ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each fin bud was first homogenized in 50 μL of Buffer RLT (supplemented with β-mercaptoethanol per kit recommendation) for 1 min at RT in a 1.5 mL centrifuge tube using a cordless motor (VWR Catalog # 47747-370) and pestle (USA Scientific Catalog # 1415-5390) ...
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bioRxiv - Plant Biology 2023Quote: ... from accessions grown in UC Davis or UC Riverside greenhouses and floated overnight in 1 mL deionized water in a 24-well tissue culture plate (VWR #10062-896) with a plastic lid to prevent evaporation of the water ...
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bioRxiv - Bioengineering 2023Quote: ... the strips were immersed in a blocking buffer (1% BSA and 0.05% Tween 20) for one hour on an orbital shaker (no. 57018-754VWR; VWR International, Brisbane, CA) set at 150 rpm and thereafter dried overnight at 4° Celsius ...
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bioRxiv - Cancer Biology 2023Quote: ... Each well is washed with 1 mL of phosphate buffered saline (PBS) prior to fixation with 500 µL of 10% buffered formalin (VWR, 89379-094). After at least 24 hours of fixation ...
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bioRxiv - Cell Biology 2023Quote: ... flies were then transferred to new vials containing a 1cm by 5cm strip of filter paper saturated with 300µl of 30% H2O2 (VWR, Cat# BDH7690-1) and placed at 25°C for 24 hours ...
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bioRxiv - Immunology 2023Quote: ... Primary CD4+ T cells were seeded at 1×106 cells/well in 24-well plates and maintained in X-VIVO-15™ media (CA12001-988, VWR, USA). Four treatment groups were used ...
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bioRxiv - Molecular Biology 2024Quote: ... The resin was equilibrated with wash buffer (50 mM Tris-HCl [Sigma-Aldrich], pH 7.5, 250 mM NaCl [Sigma-Aldrich], 1 mM DTT [VWR Chemicals]) and bound proteins were eluted in wash buffer supplemented with 20 mM glutathione ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM Tris-HCl, pH 7.2, 5 mM EGTA, 100 mM KCl, 1% Triton-X100; all chemicals from VWR; Radnor, PA, USA). Samples were homogenized using tight-fitting pestles and centrifuged at 3,000 g for 30 minutes at 4ºC ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was lysed using lysis buffer (50 mM Tris-HCl [Sigma-Aldrich], pH 7.5, 250 mM NaCl [Sigma-Aldrich], 1 mM dithiotreitol (DTT; [VWR Chemicals])) supplemented with protease inhibitor [cOmplete ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was lysed using lysis buffer (50 mM Tris-HCl [Sigma-Aldrich], pH 7.5, 250 mM NaCl [Sigma-Aldrich], 1 mM DTT [VWR Chemicals]) supplemented with protease inhibitor [cOmplete ...
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bioRxiv - Cell Biology 2024Quote: ... the coverslips were PEGylated by incubating in a 1:100 mixture of 1% biotin-PEG-silane in ethanol (Laysan Bio Biotin-PEG-SIL-2K-1g) and PEG-silane (85%, VWR 77035-498) for one hour in the dark at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Solutions were mixed by shaking the compound plate for 1 min at room temperature at 1200 rpm using a microplate shaker (cat. 12620-926, VWR, West Chester, PA). A typical plate layout map used for screening experiments is shown in Figure 1 ...