Labshake search
Citations for VWR :
701 - 750 of 771 citations for 6 chloro 1H pyrrolo 2 3 b pyridine 3 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... CO2-anethetized male or female flies that had eclosed 2 days earlier were mounted on double-sided sticky tape and wings coated in Voltalef 10S halocarbon oil (VWR). Movements of mitochondria (labeled with mito-GFP under the control of the neuronal driver appl-GAL4 ...
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bioRxiv - Microbiology 2023Quote: ... Single-species cultures were obtained by picking colonies using 1 µL sterile inoculation loops and transferred to 800 µL liquid isolation medium in 96 deep well 2 mL plates (VWR) which were incubated for at least 2 weeks on a bench top shaker (RT ...
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bioRxiv - Synthetic Biology 2023Quote: Sender strains and receiver strains were inoculated in 1 mL of rich M9 medium supplemented with the appropriate antibiotics in a 2 mL deep-well 96-well plate (VWR), covered with a “Breathe Easier” membrane (Sigma) ...
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bioRxiv - Synthetic Biology 2023Quote: Cells carrying the open-loop and closed-loop versions of the composition controller circuits were grown as monocultures in 1 mL of rich M9 medium supplemented with the appropriate antibiotics in 2 mL 96-well deep-well plates (VWR) at 30°C in a plate shaker incubator (Infors HT Multitron ...
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bioRxiv - Plant Biology 2023Quote: ... Flash chromatography was carried out using a 2 mL glass pasteur pipette loaded with 200 mg of P60 silica gel (SiliCycle) with hexane (HPLC grade; VWR) as the initial mobile phase ...
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bioRxiv - Cell Biology 2024Quote: ... This was followed by perfusion with Solution 2 consisting of DMEM supplemented with collagenase type I (0.8 mg/mL) (VWR International) for 7–8 mins at the same flow rate ...
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bioRxiv - Biochemistry 2019Quote: ... The column was held under isocratic flow for 10 mins prior to developing a linear gradient from 0 to 2 M NaCl (HPLC grade; VWR, UK) over 60 mins ...
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bioRxiv - Bioengineering 2021Quote: ... cell suspensions were prepared at a concentration of 300,000 cells/mL in 1X phosphate buffered saline (PBS) solution supplemented with 2% fetal bovine serum (FBS, VWR 89510-186) to reduce cell-cell and cell-PDMS adhesion ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then normalized to 1 ng μL-1 or 2 ng μL-1 for genomic DNA extracted from fecal and cecal samples by diluting in molecular grade water (VWR International) using a Tecan Evo Liquid Handling Robot ...
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bioRxiv - Microbiology 2020Quote: ... Both filtered and unfiltered water samples were vortexed and diluted 1:5.75 into 2% (vol/wt) trace-grade nitric acid (VWR, Radnor, PA) in acid-washed polypropylene tubes and stored overnight at ambient temperature ...
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bioRxiv - Microbiology 2020Quote: ... Pigment-expressing GBS was concentrated in 50 µL of PBS and a 2 µL droplet was aliquoted and sandwiched between two cover slides (48393-230, VWR International). Changes in pigment presence were determined by measuring the change in Raman peak amplitudes associated with conjugated double bonds located at the 1126 cm-1 and 1510 cm-1 before and after light treatment ...
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bioRxiv - Pathology 2022Quote: ... We extracted the brains and post fixed each in the same fixative overnight and then placed them in a freeze protective solution containing 2% DMSO and 20% glycerine (PB with dimethyl sulfoxide, VWR Int. LLC, Cat# 23486.297, and glycerine, VWR, Cat# 24387.292) in a refrigerator until sectioning ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to 200 µl of 50% HNO3 and heated for 2 hrs at 90°C in a loosely capped 15ml centrifuge tube (VWR, #490001), (3 ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to an empty tube and heated for 2 hr at 90°C in a loosely capped 15ml centrifuge tube (VWR, #490001).
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bioRxiv - Cancer Biology 2022Quote: ... was added to 200 µl of 50% HNO3 (prepared from trace metal grade HNO3) and heated for 2 hrs at 90°C in a loosely capped 15 ml centrifuge tube (metal free, VWR, #490001), (2 ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA was then mixed with gene-specific primers (2 μM) and Perfecta SYBR green SuperMix reagent (Cat # MP9505402K, VWR, Radnor, PA) for PCR amplification using a DNA Engine Opticon 2 system (MJ Research ...
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bioRxiv - Developmental Biology 2020Quote: ... 10% formamide and 2x saline sodium citrate (SSC) supplemented fresh each experiment with 0.1% Tween-20 and 2 μg/mL nuclease-free BSA (VWR, 0332-25G)) ...
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bioRxiv - Microbiology 2020Quote: ... The 12 lineages were evolved in 1 mL of antibiotic-free LB medium using 2 mL-deep-96-well plates in checkered pattern (VWR International) and incubated at 37°C with 700 rpm constant shaking (Microplate Shaker TiMix 5 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... each chamber was filled with 100μl food medium containing a mix of 2% agarose (VWR life science; type number: 97062-250), 2% sucrose (Merck KGaA ...
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bioRxiv - Microbiology 2019Quote: ... colonies were pooled by suspending the cells in 2 mL 0.9% NaCl per plate with a cell scraper (Corning®, VWR, Germany).
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bioRxiv - Immunology 2020Quote: ... and the filter was washed with sterile 1x phosphate buffer saline (PBS) supplemented with 2% v/v fetal bovine serum (FBS, VWR, USA). Cell density was determined through trypan blue exclusion ...
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bioRxiv - Immunology 2020Quote: ... diluted to 5 µg/ml in PBS containing 2% (w:v) Fraction V BSA bovine serum albumin (PBS-BSA) (VWR, Radnor, PA). Slides were then washed in PBS ...
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bioRxiv - Microbiology 2019Quote: ... Growth in the phylloplane was assayed by re-isolating Protomyces from inoculated leaves by placing leaves in 2 ml Eppendorf tubes with 1 ml 0.025% Silwet-L77 in water and shaking (800 rpm; VWR microplate shaker) for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... The 168 h incubation samples were sonicated for 2 minutes in a water bath sonicator (VWR® Symphony™ Ultrasonic Cleaner). Circular dichroism (CD ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were diluted in PBS with 2% FBS and samples were stored in PBS containing 1% formaldehyde (VWR Life Science AMRESCO). For intracellular staining ...
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bioRxiv - Immunology 2022Quote: ... 2-2.5 × 106 frozen or freshly isolated monocytes were seeded in 10 cm non-tissue culture treated dishes (VWR, #734-2796) in 10 mL RPMI 1640 (Biowest ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we thawed yeast RBD library and the Wuhan Hu-1 and Omicron BA.1 strains by inoculating the corresponding glycerol stocks in SDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% dextrose [VWR #90000– 904] ...
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bioRxiv - Synthetic Biology 2022Quote: ... boulardii cultures were incubated in 2 mL DELFT medium supplemented with 20 mg/L uracil in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen) ...
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bioRxiv - Synthetic Biology 2022Quote: Single and co-cultivation of (SbU-)-GFP and (SbU- + bts1Δ + thi6Δ)-mKate were cultured in 2 mL SC media YPD in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen ...
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bioRxiv - Synthetic Biology 2022Quote: Single and co-cultivation of (SbU-)-GFP and (SbU- +bts1Δ + thi6Δ)-mKate were cultured in 2 mL SC media with and without thiamine in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 mL LB or BHI medium was inoculated with 20 µL preculture and grown in a 24-well plate (VWR, USA). Furthermore ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was then neutralized using 1M HEPES Solution pH 8.0 to a neutral pH before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ~2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). Next ...
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bioRxiv - Immunology 2023Quote: ... were cultured in six-well plates at a density of 5 x 106 cells/mL in 2 mL of RPMI 1640 (cat#45000-396, VWR, USA) supplemented with 10% fetal bovine serum (Omega Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then neutralized using 1 M HEPES solution pH 8.0 before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ∼ 2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). After ...
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bioRxiv - Synthetic Biology 2022Quote: ... boulardii strains were incubated in 2 mL DELFT medium supplemented with 20 mg/L uracil in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen) ...
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bioRxiv - Microbiology 2023Quote: ... A total of 20 μg of proteins were mixed with 6x SDS loading dye containing 10% 2-mercaptoethanol (VWR Life Sciences) and boiled at 100 °C for 10 min before loading ...
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bioRxiv - Cancer Biology 2020Quote: ... 1.2 × 105 cells were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
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bioRxiv - Cell Biology 2021Quote: ... the eye cups were infiltrated with a 2:1 mixture of PBS containing 30% sucrose and OCT compound (VWR International, Radnor, PA) and frozen with dry ice ...
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bioRxiv - Biophysics 2021Quote: ... and subsequently incubated at 37 °C and 5% CO2 for 2 h before seeded and imaged on an 18 mm diameter microscope cover glass (VWR, Darmstadt, Germany) in PBS containing Ca2+ (Gibco ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Transformants were plated on SDCAA-agar (1.71 g/L YNB without amino acids and ammonium sulfate [Sigma-Aldrich #Y1251], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% dextrose [VWR #90000–904] ...
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bioRxiv - Developmental Biology 2020Quote: ... and then incubated with secondary antibodies and DAPI for 2 hr at room temperature prior to mounting in AquaPoly/Mount mounting medium (VWR, 87001-902).
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bioRxiv - Neuroscience 2019Quote: ... the flies were first sated with water and then presented with 100mM sucrose containing 2% blue dye (McCormick) filled in a graduated capillary (VWR, #53432-604). Until the flies became unresponsive to 10 serial food stimuli ...
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bioRxiv - Bioengineering 2021Quote: Plant or mammalian produced SARS-CoV-2 RBD protein were diluted in sterile 1X PBS to 2 μg/mL and coated onto a 96-well plates (VWR #62402-959) (50 μL /well ...
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bioRxiv - Microbiology 2022Quote: ... Spleen and brain were homogenized in 1 ml of sterile PBS followed by culture of 10-fold dilutions of each tissue on 2X GYE agar (2% glucose (VWR™, USA), 1% yeast extract (BD™ ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR, 75870-796) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown at 21°C in shaking culture in HL5 medium (Formedium, Norfolk, England) and on SM/5 agar plates (2 g/L glucose (VWR, Solon, OH), 2 g/L bacto peptone (Becton ...
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bioRxiv - Immunology 2023Quote: ... mice were whole body perfused through the heart with 2% paraformaldehyde (diluted down in PBS from 16%; VWR Cat. No. 15710-S), then livers were harvested and placed in 2% paraformaldehyde for 24 hours at 4°C then placed in 30% sucrose (Sigma-Aldrich Cat ...
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bioRxiv - Physiology 2023Quote: ... after overnight fasting followed by administration of a saline solution of glucose (2 g/kg or 1.5g/kg, for rats or mice, respectively; VWR Chemicals, Leuven, Belgium), by gavage ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were washed 3x for 15 minutes with PBS-T (PBS with 0.02% Tween) and incubated for 2 hours with either anti-rabbit HRP-linked (VWR, cat. no NA934) or anti-mouse HRP-linked (VWR ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Equal volumes (5 μL) of the resulting PCR product were loaded onto a 2% biotechnology grade Agarose I (VWR International, PA, USA) gel prepared with 1X TBE buffer and 0.0025% ethidium bromide ...