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Citations for VWR :
451 - 500 of 637 citations for Rat Butyrophilin Like Protein 2 BTNL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... an LD50 culture was prepared by pipetting 100 μL of the diluted expression culture into each cavity of a 96-deep well plate (96 square well, 2 mL, VWR) containing 400 μL of expression media (final OD600 = 0.02) ...
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bioRxiv - Genomics 2022Quote: ... multimodal slides with leaf sections attached to the capture areas were incubated for 2 minutes at 37°C followed by a 40-minute fixation in methanol (VWR) at -20°C ...
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bioRxiv - Immunology 2022Quote: ... Biotin+ or total DCs were sorted into U bottom 96well plate containing supplemented RPMI and 2% of T-stim media (VWR). 750 naïve CD4+ OT-II CFSE-labeled T cells from the spleen were sorted into the corresponding wells ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL of each rescuer strain was inoculated into 500 μL of SC -his media in 96 well 2 mL deep well plate format (VWR) and shaken at 900 rpm at 30°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Resistance selection using fixed concentrations of COV-siRNA1 or COV-siRNA1+2 was conducted in Vero E6 cells cultured in DMEM supplemented with 10% FBS (VWR) and 1x Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Formic acid (FA), 2-proponol, acetonitrile (ACN), and methanol (all HiPerSolv CHROMANORM, HPLC grade, BDH Prolabo) were purchased from VWR International (Helsinki ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Plant Biology 2023Quote: ... Flash chromatography was carried out using a 2 mL glass pasteur pipette loaded with 200 mg of P60 silica gel (SiliCycle) with hexane (HPLC grade; VWR) as the initial mobile phase ...
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bioRxiv - Developmental Biology 2023Quote: ... All PCR products were separated by electrophoresis on a 2% agarose gel in TBS stained with ethidium bromide (VWR, E3050), and genotypes determined based on sizes of DNA products.
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: The brain and lung tissue were transferred to a cold room and subjected to homogenization using a 2 ml Dounce homogeniser (VWR, Tissue grinders ...
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bioRxiv - Biochemistry 2023Quote: ... were obtained from MilliporeSigma. Tris(2-carboxyethyl)phosphine (TCEP) hydrochloride (Cat. # K831, CAS: 51805-45-9) was purchased from VWR. Tris[(1-benzyl-1H-1,2,3-triazol-4- yl)methyl]amine (TBTA ...
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bioRxiv - Microbiology 2024Quote: ... smegmatis WT and ΔmftG strains were grown in 320 mL HdB-Tyl medium supplemented with 1 g L−1 ethanol as the sole carbon source in 2 L flasks covered by breathable rayon film (VWR) in triplicates and incubated at 37 °C and 210 rpm with a starting OD600 of 0.1 for WT and 2.0 for ΔmftG for a period of 48 h.
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bioRxiv - Microbiology 2024Quote: ... The main culture was cultivated as 10x 500 mL cultures in 2 L shake flasks sealed with breathable rayon film (VWR) for reproducible aeration for 72 h ...
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bioRxiv - Cell Biology 2024Quote: ... 1:1000 LIF) and were pre-plated for 1-2 h to remove fibroblasts before they are seeded on matrigel (VWR) coated dishes ...
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bioRxiv - Synthetic Biology 2023Quote: ... Routine culture of E.coli for cloning purposes was performed in Miller’s LB Broth (Fisher BP1426-2) in 14mL Falcon tubes (VWR 60819-761) at 37°C on a rotating drum ...
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bioRxiv - Microbiology 2023Quote: ... Lungs were transferred to a 2 mL tube pre-filled with 2.8 mm ceramic beads (VWR, Cat. No. 10158-612), flash frozen on dry ice ...
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bioRxiv - Cell Biology 2023Quote: ... CO2-anethetized male or female flies that had eclosed 2 days earlier were mounted on double-sided sticky tape and wings coated in Voltalef 10S halocarbon oil (VWR). Movements of mitochondria (labeled with mito-GFP under the control of the neuronal driver appl-GAL4 ...
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bioRxiv - Immunology 2023Quote: MDCK.2 cells (ATCC) were seeded at a density of 1 × 106 cells/well in 6-well plates (VWR International) and incubated overnight at 37℃ 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... Single-species cultures were obtained by picking colonies using 1 µL sterile inoculation loops and transferred to 800 µL liquid isolation medium in 96 deep well 2 mL plates (VWR) which were incubated for at least 2 weeks on a bench top shaker (RT ...
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bioRxiv - Biochemistry 2022Quote: ... 50 µL of each was added to 2 mL cultures of attached cells in 6-well plates (#10861-554, VWR) containing 0.2 mM CaCl2 ...
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bioRxiv - Biochemistry 2022Quote: Each <3 kDa FBS extracted material was added to >30 kDa FBS fraction in 2 mL FBS-free media in the well of a 6-well plate (#10861-554, VWR) at roughly 10X the concentration they would exist in the standard growth media ...
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bioRxiv - Synthetic Biology 2023Quote: Sender strains and receiver strains were inoculated in 1 mL of rich M9 medium supplemented with the appropriate antibiotics in a 2 mL deep-well 96-well plate (VWR), covered with a “Breathe Easier” membrane (Sigma) ...
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bioRxiv - Synthetic Biology 2023Quote: Cells carrying the open-loop and closed-loop versions of the composition controller circuits were grown as monocultures in 1 mL of rich M9 medium supplemented with the appropriate antibiotics in 2 mL 96-well deep-well plates (VWR) at 30°C in a plate shaker incubator (Infors HT Multitron ...
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bioRxiv - Microbiology 2023Quote: ... 4 °C) and the cells were resuspended in 5 mL of methanol/dichlormethane/ethylacetate 10:2:3 (dichlormethane: SupraSolv, VWR Chemicals ...
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bioRxiv - Cell Biology 2024Quote: ... This was followed by perfusion with Solution 2 consisting of DMEM supplemented with collagenase type I (0.8 mg/mL) (VWR International) for 7–8 mins at the same flow rate ...
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bioRxiv - Neuroscience 2022Quote: RNA was isolated using TriFast RNA extraction kit (30-2030, VWR) or RNA-Solv reagent (Omega Bio-tek) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Total RNA Kit I (Omega BIO-TEK; VWR catalog no. 101319) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: DNA was purified using the yeast genomic DNA purification kit (VWR). Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... The COD was determined using the Spectroquant COD kit (VWR, Germany) according to the manufacturer’s guidelines ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we first extracted yeast genomic DNA using DNA purification kit (VWR). Genomic library preparation using Illumina DNA Prep kit and sequencing using Illumina NovaSeq 6000 and NovaSeq X Plus sequencer with 150-bp paired-end reads were performed by the Microbial Sequencing Center at University of Pittsburgh ...
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bioRxiv - Cell Biology 2019Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue sections were co-stained overnight at 4°C with a primary chicken antibody against the astrocyte marker Glial Fibrillary Acidic Protein (GFAP) (1:1000 in blocking solution; Cat No. 200-901-D60, VWR, Radnor, PA, USA) and a primary rat antibody against myelin basic protein (MBP ...
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bioRxiv - Biochemistry 2019Quote: ... The column was held under isocratic flow for 10 mins prior to developing a linear gradient from 0 to 2 M NaCl (HPLC grade; VWR, UK) over 60 mins ...
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bioRxiv - Bioengineering 2021Quote: ... cell suspensions were prepared at a concentration of 300,000 cells/mL in 1X phosphate buffered saline (PBS) solution supplemented with 2% fetal bovine serum (FBS, VWR 89510-186) to reduce cell-cell and cell-PDMS adhesion ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then normalized to 1 ng μL-1 or 2 ng μL-1 for genomic DNA extracted from fecal and cecal samples by diluting in molecular grade water (VWR International) using a Tecan Evo Liquid Handling Robot ...
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bioRxiv - Microbiology 2020Quote: ... Both filtered and unfiltered water samples were vortexed and diluted 1:5.75 into 2% (vol/wt) trace-grade nitric acid (VWR, Radnor, PA) in acid-washed polypropylene tubes and stored overnight at ambient temperature ...
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bioRxiv - Microbiology 2020Quote: ... Pigment-expressing GBS was concentrated in 50 µL of PBS and a 2 µL droplet was aliquoted and sandwiched between two cover slides (48393-230, VWR International). Changes in pigment presence were determined by measuring the change in Raman peak amplitudes associated with conjugated double bonds located at the 1126 cm-1 and 1510 cm-1 before and after light treatment ...
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bioRxiv - Pathology 2022Quote: ... We extracted the brains and post fixed each in the same fixative overnight and then placed them in a freeze protective solution containing 2% DMSO and 20% glycerine (PB with dimethyl sulfoxide, VWR Int. LLC, Cat# 23486.297, and glycerine, VWR, Cat# 24387.292) in a refrigerator until sectioning ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were plated in 6-well plates at 1 million cells per well and the following day were transfected with 2 μg DNA using 6 μl of JetPrime (VWR International) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to 200 µl of 50% HNO3 and heated for 2 hrs at 90°C in a loosely capped 15ml centrifuge tube (VWR, #490001), (3 ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to an empty tube and heated for 2 hr at 90°C in a loosely capped 15ml centrifuge tube (VWR, #490001).
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bioRxiv - Cancer Biology 2022Quote: ... was added to 200 µl of 50% HNO3 (prepared from trace metal grade HNO3) and heated for 2 hrs at 90°C in a loosely capped 15 ml centrifuge tube (metal free, VWR, #490001), (2 ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA was then mixed with gene-specific primers (2 μM) and Perfecta SYBR green SuperMix reagent (Cat # MP9505402K, VWR, Radnor, PA) for PCR amplification using a DNA Engine Opticon 2 system (MJ Research ...
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bioRxiv - Developmental Biology 2020Quote: ... 10% formamide and 2x saline sodium citrate (SSC) supplemented fresh each experiment with 0.1% Tween-20 and 2 μg/mL nuclease-free BSA (VWR, 0332-25G)) ...
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bioRxiv - Microbiology 2020Quote: ... The 12 lineages were evolved in 1 mL of antibiotic-free LB medium using 2 mL-deep-96-well plates in checkered pattern (VWR International) and incubated at 37°C with 700 rpm constant shaking (Microplate Shaker TiMix 5 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... each chamber was filled with 100μl food medium containing a mix of 2% agarose (VWR life science; type number: 97062-250), 2% sucrose (Merck KGaA ...
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bioRxiv - Microbiology 2019Quote: ... colonies were pooled by suspending the cells in 2 mL 0.9% NaCl per plate with a cell scraper (Corning®, VWR, Germany).
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bioRxiv - Immunology 2020Quote: ... and the filter was washed with sterile 1x phosphate buffer saline (PBS) supplemented with 2% v/v fetal bovine serum (FBS, VWR, USA). Cell density was determined through trypan blue exclusion ...
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bioRxiv - Immunology 2020Quote: ... diluted to 5 µg/ml in PBS containing 2% (w:v) Fraction V BSA bovine serum albumin (PBS-BSA) (VWR, Radnor, PA). Slides were then washed in PBS ...