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Citations for Zymo Research :
1 - 50 of 543 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... and single colonies miniprepped and used for transfection(Zymo Research, Cat. No. D4208T).
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bioRxiv - Molecular Biology 2020Quote: ... Bulk controls were sorted into 75 μl DNA/RNA Shield (Zymo Research, cat. R1100-50). Following sorting ...
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bioRxiv - Microbiology 2023Quote: ... Controls samples included an 8-member positive controls (Zymo Research), as well as an extraction negative and a PCR negative controls ...
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bioRxiv - Microbiology 2023Quote: ... Negative extraction controls (NECs) as well as mock-community positive controls (Zymo Research) were included.
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bioRxiv - Neuroscience 2024Quote: ... A negative control with the control from DNA extraction and a positive control of ZymoBIOMICS™ Microbial Community Standard (ZYMO Research, Irvine, CA) were set during amplicon library preparation ...
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bioRxiv - Genomics 2021Quote: ... The negative control (ZYMO-UM) DNA in the set comes from a Human HCT116 DKO Non-methylated DNA that contains genetic knockouts of both DNA methyltransferases DNMT1 (-/- ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids used for transient transfections in HEK293T cells were prepared and purified using the Zymopure Midiprep Kit (Zymo Research, cat #D4201). Mutations to create the CFTR ΔPRF constructs were chosen using RNA structure predictions performed by the ViennaRNA RNAfold Web Server.
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bioRxiv - Genomics 2022Quote: ... separating 22 or 10 (control wells) single nuclei per well in a 96-well plate containing 2.5 μL of M-digestion buffer (Zymo, Cat. D5020-9), 0.25 μL of Proteinase K (Zymo ...
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bioRxiv - Genomics 2023Quote: ... ∼15 ng of genomic DNA from each sample was first spiked in with in vitro methylated lambda phage genomic DNA (0.2%) as controls and was then subjected to bisulfite conversion (EZ DNA Methylation-Direct Kit, Zymo Research Cat# D5020). Half of the bisulfite converted DNA was used for low input WGBS analysis (for 5mC+5hmC profiling) ...
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bioRxiv - Cell Biology 2021Quote: ... Transfections were performed with PEI and 2-5 µg of transfection grade DNA (ZymoPure Plasmid MaxiPrep Kit, Zymo) per dish as described in above ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... One negative extraction control (no sample added) and one positive extraction control (ZymoBIOMICS Microbial Community Standard; Zymo Research) were processed in parallel during the DNA extraction procedures and subsequently sequenced ...
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bioRxiv - Microbiology 2020Quote: ... a Zymo positive control community (Zymo Research) and E ...
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bioRxiv - Plant Biology 2019Quote: ... One negative water control and one mock community DNA control (ZymoBIOMICS Microbial Community Standard, Zymo Research, Irvine, CA, USA) were included on each plate ...
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bioRxiv - Microbiology 2024Quote: ... Every extraction batch of 96 samples included one water blank as a negative control and one mock community aliquot (Zymo Research; Cat. No. D6300) as a positive control ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial DNA was extracted from the fecal and intestinal samples and from two negative extraction controls and two ZymoBIOMICS Microbial Community Standard positive extraction controls (D6300 Zymo Research) with the DNeasy PowerSoil Pro Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... containing a known concentration and taxonomy of 8 bacterial and 2 fungal species was also included as an internal control (Zymo Research Corp, Cat No. D6310). Extracted nucleic acids from each sample were randomized into a 96-well rack (Figure 1C ...
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bioRxiv - Microbiology 2023Quote: ... negative controls (empty tubes) and positive controls (D6300 ZymoBIOMICS Microbial Community standard and D6305 ZymoBIOMICS Microbial Community DNA standard, Zymo Research, USA) were included ...
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bioRxiv - Microbiology 2022Quote: ... four positive controls (Zymobiomics microbial community standard (Zymo Research)) were amplified with this protocol ...
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bioRxiv - Immunology 2023Quote: ... Negative and positive controls (ZymoBIOMICS D6300 & D6305, Zymo, CA) were included in each round of extraction and PCR ...
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bioRxiv - Bioengineering 2024Quote: ... Blank controls and mock communities (Zymo Research Corporation, USA) were included and carried through all downstream processing steps as quality control ...
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bioRxiv - Microbiology 2022Quote: ... Reagent blanks were included as negative controls and cells from a microbial community of known composition were included as positive controls (ZymoBiomics Microbial Community Standards; Zymo Research, Irvine, CA). The V4 region of the 16S rRNA gene was amplified from approximately 5 ng of extracted DNA in 25μl reactions using Q5 HS High-Fidelity polymerase (New England BioLabs ...
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bioRxiv - Cell Biology 2020Quote: ... DNA was purified for transfection using Zymopure midiprep kits (Zymo Research). Plasmid transfections were accomplished using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... 48 hours post-transfection cells were collected in Tri-reagent (Zymo) or RIPA buffer supplemented with protease inhibitors and 96-well plates were fixed with 4% paraformaldehyde.
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bioRxiv - Neuroscience 2020Quote: ... TUNEL positive control experiment was performed using DNase LJ (ZYMO RESEARCH, #E1010)-treated brains.
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bioRxiv - Systems Biology 2023Quote: ... We also included seven negative controls containing only Shield™ (Zymo Research) in the extractions ...
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bioRxiv - Molecular Biology 2023Quote: ... Colonies were scraped from the bioassay dishes and midi-prepped for transfections (Zymo).
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bioRxiv - Microbiology 2019Quote: ... DNA extraction kit controls and ZymoBIOMICS mock communities (Zymo Research, Irvine, CA, USA) were included alongside the soil DNA and sequenced ...
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bioRxiv - Genomics 2021Quote: cfDNA and nucleic acid controls were bisulfite treated according to manufacturer recommendations (Zymo Methylation Lightning Kit ...
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bioRxiv - Genomics 2020Quote: ... We prepared the library plasmid for transfection using the ZymoPURE Plasmid Maxiprep Kit (Zymo).
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bioRxiv - Genomics 2022Quote: ... DNA suitable for transfection was prepped using the ZymoPURE maxiprep kit (Zymo Research D4203) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: ... The mock bacterial community control yielded the expected community composition as specified by Zymo Research.
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bioRxiv - Microbiology 2021Quote: ... Two positive sequencing controls (ZymoBiomics Microbial Community DNA Standards; Zymo Research, Irvine, California, USA) and two negative sequencing controls (sterile water ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... Total RNA was harvested at various times post transfection/treatment with RNA-extraction kit (Zymo) and reverse transcribed with MLV-RT (Invitrogen ...
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bioRxiv - Genomics 2021Quote: Plasmids used for transfections were purified using ZymoPURE II Plasmid Midiprep Kit (Zymo Research, D4201). Plasmids were linearized using PvuI-HF (NEB ...
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bioRxiv - Genomics 2023Quote: DNA suitable for transfection was prepped using the Zymo-PURE maxiprep kit (Zymo Research D4203) according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2022Quote: ... DNA from pre-sort control and sorted cells was extracted with Microprep DNA kits (Zymo Research) and triple-eluted with water ...
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bioRxiv - Genomics 2020Quote: ... Endo-free plasmids used for mammalian transfection were prepped using ZymoPURE II Plasmid Midiprep (Zymo Research Corporation) from 50 mL Mach1 (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA was isolated 72 hr post-transfection using the Quick-DNA Miniprep Plus kit (Zymo Research) and eluted into 25 μl 10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... pLXSP-16E7 or control vector infected primary mouse keratinocytes with the Quick-RNA Miniprep kit (Zymo Research). cDNA was synthesized with the Quantinova Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... we applied the same procedures to a positive control (eight species Zymo mock community, Zymo Research, USA) and six negative controls (two for each DNA extraction kits ...
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bioRxiv - Microbiology 2020Quote: ... Positive controls were provided by extracts of a ZymoBIOMICS Microbial Community Standard (D6300; Zymo Research, Irvine, CA). Bacteria were characterized using PCR that targeted the V3 and V4 portion of the 16S rRNA gene to amplify that gene segment for subsequent Illumina MiSeq analysis ...
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bioRxiv - Microbiology 2022Quote: ... we applied the same procedures to a positive control (eight species Zymo mock community, Zymo Research, USA) and six negative controls (two for each DNA extraction kits ...
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bioRxiv - Genetics 2020Quote: ... and total RNA was extracted 36 hours post-transfection using the Quick-RNA MiniPrep Plus kit (ZYMO Research). Using a primer specific to the 3′ native exon of the pET01 vector ...
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bioRxiv - Cancer Biology 2019Quote: RNA samples were extracted from cells three days post DsiRNA transfection using Quick-RNA Microprep Kit (Zymo Research), treated with DNase I (Zymo Research) ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from test and control mosquito samples using the Direct-zol RNA Miniprep Kit (Zymo Research). The positive and negative controls for the RT-PCR were RNA extracted from an FFA-confirmed EILV-eGFP positive mosquito and an FFA-confirmed EILV-eGFP negative mosquito (EILV-eGFP challenged) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs of cells expressing CRISPRi or other transfection were performed using Quick-RNA Miniprep Kit (Zymo Research, 11-328). Reverse transcription was conducted by using Superscript™ IV first strand synthesis kit (Thermo Fisher ...
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bioRxiv - Genetics 2020Quote: ... Cells were harvested 36h after transfection and total RNA was extracted using Quick-RNA MiniPrep Plus kit (ZYMO Research). cDNA was transcribed using 750ng of isolated RNA SuperScript™ III Reverse Transcriptase (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA was harvested from cells 24-hours post-transfection using the Direct-zol RNA Miniprep kits (Zymo Research). Each in vivo splicing assay was performed a minimum of three times.
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bioRxiv - Microbiology 2023Quote: ... Plasmid DNA was purified for transfection into mammalian cell lines using a ZymoPURE II Plasmid Maxiprep Kit (Zymo Research).
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bioRxiv - Systems Biology 2022Quote: ... A no-template control and a ZymoBIOMICS D6305 microbial community DNA standard were included (Zymo Research, Irvine, CA USA). Paired-end 300 bp sequencing was performed on an Illumina MiSeq PE300 (v3 mode ...