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Citations for Zymo Research :
1 - 50 of 64 citations for Siglec 15 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Total RNA was extracted from mouse tissues and HEK293 cells using Quick-RNA MiniPrep Kit (ZYMO Research, Irvine, CA). Five μg of treated RNA was used as template for first-strand cDNA synthesis by using RevertAid RT Reverse Transcription Kit (Life Technologies ...
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bioRxiv - Systems Biology 2019Quote: ... SD -His with uracil and 5FOA (Zymo Research) was used to analyze the phase separation effects of a toxic Ura3p activity.
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from HEK293 and CDK9as cells using a Quick-RNA Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 15 µl EZ Methylation Direct conversion reagent (Zymo Research) was added ...
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bioRxiv - Biochemistry 2020Quote: ... then loaded directly onto a Ni-NTA protein miniprep column (His Spin Protein Miniprep, Zymo Research). Protein was washed following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The cells were harvested and the protein was purified using His-Spin Protein Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... the DNMT3A KO and WT HEK293 cells were cultured and RNA samples were extracted using Direct-zol RNA kit (Zymo Research; Irvine, CA, USA). Then cDNA was synthesized according to the protocol of the RT-PCR kit (Takara ...
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bioRxiv - Microbiology 2022Quote: ... After collection of TI and TS fractions 100 µl of His-affinity gel (i.e., Ni-NTA Magnetic Agarose Beads) (ZYMO Research) and 10 µl of protease inhibitor cocktail (Sigma ...
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bioRxiv - Genomics 2021Quote: ... Purified bisulfite-treated DNA was eluted in 15 mL of M-Elution Buffer (Zymo Research). DNA methylation levels were measured using Infinium Human Methylation 450 arrays (Illumina ...
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bioRxiv - Synthetic Biology 2021Quote: ... The reaction was stopped by proceeding to RNA purification with elution in 15 μL (R1013, Zymo Research). Polyadenylated RNA (1 μg ...
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bioRxiv - Synthetic Biology 2022Quote: ... 15 μl of PCR products were pooled and purified using PCR Purification Kit (D4013, Zymo Research Co.). Depending on the amplicon ...
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bioRxiv - Plant Biology 2019Quote: ... benthamiana plants was purified from upper non-inoculated leaves at 15 dpi using silica-gel columns (Zymo Research). For virus infection diagnosis ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and bodies (thorax minus legs + abdomen) were dissected and pooled from 15 individuals in DNA/RNA shield reagent (Zymo) and stored at -20°C until further processing ...
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bioRxiv - Developmental Biology 2021Quote: ... or Universal Methylated Mouse DNA Standard (Zymo Research) was bisulfite converted using EZ DNA Methylation-GoldTM Kit (Zymo Research) ...
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bioRxiv - Immunology 2021Quote: ... followed by a 15 minute treatment with DNase and subsequent purification with Direct-zol™ RNA MiniPrep Plus (Zymo Research). All shRNAs used in this study can be found in Supplementary Dataset 2.
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bioRxiv - Molecular Biology 2022Quote: ... The assembly is cleaned and concentrated to 15 μl mQ using the DNA Clean & Concentrator™-5 kit (Zymo, D4004). 3 μl Gel Loading Dye ...
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bioRxiv - Microbiology 2023Quote: ... 15 min) and total RNA was extracted and DNAseI-treated using the Direct-zol RNA miniprep plus kit (Zymo Research). RNA quality was verified by 1% agarose electrophoresis and quantified using Nanodrop One (Thermo Scientific) ...
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bioRxiv - Immunology 2024Quote: CP-KO RNA was extracted from 15 3h PBF guts with the Direct-zol RNA Mini-prep kit (Zymo Research) including on-column DNase treatment and transcribed into cDNA with the qScript cDNA Synthesis Kit (Quantabio) ...
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bioRxiv - Genomics 2020Quote: ... 5 µl reactions were purified with the ZR-96 Oligo Clean & Concentrator and 15 µl reactions were purified with the Oligo Clean & Concentrator kit (Zymo Research) and eluted with probe resuspension buffer ...
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bioRxiv - Genetics 2021Quote: DNA samples ranging from 500 ng-2 μg were digested to free nucleosides using 5-15 U of DNA Degradase Plus (Zymo Research) in 25 μl reactions incubated for 2 hrs at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... The reactions were treated with DNAse (15 min at 37°C) and remaining RNA was recovered using Direct-zol RNA mini prep kit (Zymo research) according to the protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The extracted RNA was treated with DNase I for 15 minutes at 37°C and further purified using a Quick-RNA miniprep kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2023Quote: The 2 μL of EVs were incubated at room temperature for 15 min with 1 U of DNase I (Zymo Research) and 1x DNA Digestion Buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was purified from a volume of lysate equivalent to 15 mg of tissue (525 uL) using a Quick-RNA Miniprep kit (Zymo Research). 500 ng of total RNA was used to generate libraries ...
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bioRxiv - Bioengineering 2024Quote: Total RNA was extracted from SCD or HD HSPCs (15, 24, 48, 72 hours and 6 days post-transfection) using the Quick-DNA/RNA Miniprep (ZYMO Research). RNA was treated with DNase using the DNase I kit (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: The 10 μL of eEVs were incubated at room temperature for 15 min with 1 U of DNase I (Zymo Research) and DNA Digestion Buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Universally methylated mouse DNA standard (cat. no. D5012, Zymo Research) and Mlh1−/− tissues were used as Mlh1+/+ and Mlh1−/− allele controls ...
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bioRxiv - Genomics 2021Quote: ... 500 µL of supernatant was transferred to a new 15 mL falcon tube containing 3.25 mL of ammonium bisulfite solution (Zymo Research, product #5030) and heated to 98°C for 10 minutes ...
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bioRxiv - Genomics 2021Quote: ... 500 µL of supernatant was transferred to 15 mL falcon tube containing 3.25 mL of ammonium bisulfite solution (Zymo Research, product #5030) and shaken in a thermomixer at 98°C for 10 minutes (15s on/30s off) ...
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bioRxiv - Molecular Biology 2022Quote: ... The digested codon random mRFP with singlestranded overhangs was cleaned and concentrated to 15 μl mQ using the DNA Clean & Concentrator™-5 kit by Zymo (D4004). The complete 15 μl containing the codon random mRFP library was used in a ligation reaction to generate the plasmid library.
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bioRxiv - Genetics 2019Quote: ... Small RNAs were sized selected and purified from a 15% denaturing polyacrylamide-urea gel using the ZR small-RNA™ PAGE Recovery Kit (Zymo Research). For oxidized RNA library preparations ...
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bioRxiv - Genetics 2022Quote: ... 30 ng of purified dsDNA was tagmented at 55 °C for 15 min and purified using DNA Clean & Concentrator-5 kit (Zymo Research, D4004) The tagmented DNA was PCR amplified using Nextera primers ...
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bioRxiv - Physiology 2023Quote: ... and immediately lysed with TRIzol (Fischer Scientific, 15-596-018) for RNA isolation using the Direct-zol RNA MicroPrep Kit (Zymo Research, R2062) per the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... chromatin was de-crosslinked for 15 minutes at 65°C and purified using the ChIP DNA Clean & Concentrator kit (Zymo Research, Freiburg, Germany). Binding of Msh2 ...
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bioRxiv - Systems Biology 2023Quote: ... The reactions were incubated for 15 min at 50 °C and 3 μl were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Molecular Biology 2020Quote: ... Universally methylated mouse DNA standard (cat. no. D5012, Zymo Research, Irvine, CA) and genomic DNA extracted from normal spleen was used as positive and negative controls ...
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bioRxiv - Genomics 2019Quote: 200 ng of mouse E14 genomic DNA sheared to 15 kb fragments were treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA, USA Research), or an EpiTect Bisulfite Kit (QIAGEN ...
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bioRxiv - Cell Biology 2020Quote: ... and primary mouse keratinocytes was isolated using Direct-zol RNA Microprep Kits (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: RNA from mouse lungs was extracted using RNA Miniprep Plus Kit (Zymo Research). Briefly ...
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bioRxiv - Cell Biology 2023Quote: gDNA was isolated from mouse gastrocnemius using Quick-DNA Microprep Plus Kit (Zymo D4074). gDNA was amplified using CloneAmp™ HiFi PCR Premix (Takara 639298 ...
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bioRxiv - Microbiology 2023Quote: ... RNA was isolated from 5µl of mouse serum using the RNA Clean & Concentrator -5 (Zymo) or High Pure Viral Nucleic Acid kit (Roche ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was isolated from mouse kidneys using Direct-zolTM RNA MiniPrep Plus (Zymo Research). 1 µg of RNA was reverse-transcribed using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems by Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from mouse kidneys using Direct-zol™ RNA MiniPrep Plus (Zymo Research). 2 µg of RNA was reverse-transcribed using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... and total RNA of mouse organoids was isolated using Quick-RNA Miniprep Plus Kit (Zymo Research), and all of them were assessed for purity and quantity using a Nanodrop 1000 spectrophotometer ...
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bioRxiv - Molecular Biology 2021Quote: ... pLXSP-16E7 or control vector infected primary mouse keratinocytes with the Quick-RNA Miniprep kit (Zymo Research). cDNA was synthesized with the Quantinova Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Genomics 2020Quote: Total RNA was isolated from mouse cochlea sensory epithelia using the Direct-zolTM RNA MiniPrep (Zymo Research). The RNA was quantified using a Nano-drop® (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from mouse hearts using either the Direct-zol RNA Miniprep kit (Zymo, cat# R2051) or the RNeasy Fibrous Tissue Mini Kit (QIAGEN ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was extracted from DIV12 infected mouse cortical neurons using the Direct-zol RNA microprep (Zymo). Ten µg were reverse-transcribed into cDNA by Maxima First Strand cDNA Synthesis Kit (Thermofischer) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from mouse hippocampus with the Quick-RNA Miniprep Plus Kit (Zymo Research, R1058) and RNA integrity was determined using Agilent Bioanalyzer ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA (400 ng) from mouse tail was bisulphite converted with the EZ DNA Methylation-Lightning kit (Zymo Research ...