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Citations for Zymo Research :
1 - 50 of 132 citations for Rnase 3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: ... SD -His with uracil and 5FOA (Zymo Research) was used to analyze the phase separation effects of a toxic Ura3p activity.
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bioRxiv - Molecular Biology 2023Quote: ... 40 μg/ml RNase A (Zymo Research, USA)) and incubated for 40 min at 37 °C in the dark ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and RNase-free DNase kit (Zymo catalog #E1010) and cDNA libraries were generated using iScript cDNA synthesis kit (Biorad catalog #1708890 ...
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from HEK293 and CDK9as cells using a Quick-RNA Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 50 μl of DNase/RNase-Free Water (Zymo Research) was added directly to the column matrix which was then centrifuged ...
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bioRxiv - Microbiology 2022Quote: ... followed by treatment with RNase-free DNAse I (Zymo Research). Complementary DNA (cDNA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 0.2μL of 25mg/mL RNAse A (Zymo research #E1008-8) was used for each sample ...
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bioRxiv - Plant Biology 2023Quote: ... and treated with the RNase-free DNase Set (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... and treated with the RNase-free DNase Set (Zymo Research) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... Total RNA was extracted from mouse tissues and HEK293 cells using Quick-RNA MiniPrep Kit (ZYMO Research, Irvine, CA). Five μg of treated RNA was used as template for first-strand cDNA synthesis by using RevertAid RT Reverse Transcription Kit (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were treated with 100 μg/ml of RNase (Zymo research) for 15 mins followed by incubation with 50 μg/ml propidium iodide (PI ...
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bioRxiv - Biochemistry 2020Quote: ... then loaded directly onto a Ni-NTA protein miniprep column (His Spin Protein Miniprep, Zymo Research). Protein was washed following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was eluted in RNAse/DNAse-free water (Zymo Research, W1001-30) and submitted to the University of Chicago Genomics Facility for further analysis.
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bioRxiv - Microbiology 2020Quote: ... RNase R (Lecigen) treatment and follow-up purification (RNA Clean and Concentrator, Zymo) was performed as described in (36) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA was removed from extractions with RNAse A (E1008, Zymo Research, Irvine, CA), and extractions were further purified and concentrated with Genomic DNA Clean and Concentrate (D4011 ...
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bioRxiv - Microbiology 2019Quote: ... The cells were harvested and the protein was purified using His-Spin Protein Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... the DNMT3A KO and WT HEK293 cells were cultured and RNA samples were extracted using Direct-zol RNA kit (Zymo Research; Irvine, CA, USA). Then cDNA was synthesized according to the protocol of the RT-PCR kit (Takara ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was eventually eluted in 25 μL of DNase/RNase-free water (Zymo Research). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... we advise RNase I RPFs be first subjected to Oligo Clean and Concentrate (Zymo Research) purification before T4-PNK dephosphorylation.
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bioRxiv - Genomics 2021Quote: ... The next morning, 8µl of RNAse A (10mg/ml, resuspended in ddH-2O) (Zymo, Cat #E1008) was added ...
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bioRxiv - Microbiology 2022Quote: The ZymoBIOMICS human fecal reference sample (Zymo D6323) was thawed at room temperature and 100 μL was taken and centrifuged at 35g for 20 minutes to separate cells from large fecal particles ...
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bioRxiv - Microbiology 2022Quote: ... After collection of TI and TS fractions 100 µl of His-affinity gel (i.e., Ni-NTA Magnetic Agarose Beads) (ZYMO Research) and 10 µl of protease inhibitor cocktail (Sigma ...
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bioRxiv - Genomics 2022Quote: ... total RNA was isolated in RNase-free water using Zymoclean RNA Clean & Concentrator™ (Zymo Research Corp.). Filtered high-quality reads were aligned to C ...
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bioRxiv - Neuroscience 2023Quote: ... RNase R-treated RNAs were then re-isolated by RNA Clean & Concentrator™-5 (Zymo Research R1014) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Methylated and unmethylated human gDNAs (Zymo Research, Irvine, CA) were bisulfite-converted using the EZ DNA Methylation-Gold kit (Zymo Research ...
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bioRxiv - Genomics 2021Quote: ... The Human Methylated & Non-methylated DNA Set (ZYMO Research) was used to generate DNA methylation standards ...
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bioRxiv - Cancer Biology 2024Quote: ... PBS cell suspension was pipetted to a pre-chilled to 1.5 mL RNase-free snap lid tube (Zymo Research ...
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bioRxiv - Neuroscience 2023Quote: OE from 3 controls and 3 sFlt1 mice was dissected and immediately placed in RNA lysis buffer (Zymo Research R1051) without vortexing for 5min and further processed according to manufacturer’s guidelines ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA from six whole 20-21 dpf offspring (3 control offspring, 3 treatment offspring) was extracted using a Zymo Duet extraction kit (Zymo, NZ). The integrity of RNA samples was determined using an Agilent RNA 6000 Nano chip on an Agilent 2100 Bioanalyzer to check that the samples had an RNA Integrity Number (RIN ...
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bioRxiv - Molecular Biology 2022Quote: ... The solution was diluted to 10% RNALater by the addition of 270μL RNase-free water (Zymo Research, Irvine, CA), vortexed gently ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA pellets were resuspended in RNAse-free water and digested with DNAseI for 20 min at room-temperature (Zymo). DNAseI-treated RNA was purified with a RNA-clean&concentrator-25 kit (Zymo) ...
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bioRxiv - Bioengineering 2023Quote: ... Triplicate RNA samples from each condition were diluted to 20 ng/μl with DNase/RNase-Free Water (ZYMO RESEARCH). 15μl RNA solution of each replicate were sent to the UCLA Center for Systems Biomedicine for RNA expression assay with the Nanostring™ nCounter ...
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bioRxiv - Systems Biology 2023Quote: ... 600 µL DNA/RNA wash buffer (Zymo, #D7010-3), and 400 µL DNA/RNA wash buffer ...
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bioRxiv - Genomics 2024Quote: Neutralization Buffer (Zymo Research, cat. no. D4027-3-50)
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bioRxiv - Immunology 2020Quote: ... whole zebrafish larvae or human biopsies using TRI Reagent (Zymo Research) or TRIzol (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... total RNA was eluted from the column in 60 uL of Buffer AVE (QIAmp) or from the beads in 30 uL of DNase/RNase-free water (Zymo) and immediately used for RT-qPCR and library preparation ...
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bioRxiv - Molecular Biology 2023Quote: ... The solution was diluted to 10% RNALater by the addition of 270 µL RNase-free water (Zymo Research, Irvine, CA), vortexed gently ...
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bioRxiv - Neuroscience 2021Quote: ... RNA extraction product was diluted 20-fold in DNase/RNase free water (from Zymo Research Direct-zol™ RNA MiniPrep Kit) before recording the UV absorbance spectra on a Hewlett Packard 8452 Diode Array Spectrophotometer ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were treated with RNase for 1 hour and proteinase K for 1 hour before ChIP DNA Clean & Concentrator (Zymo Research) to extract the DNA.
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bioRxiv - Cell Biology 2023Quote: ... The RNAse treatments were carried out for 20 min at 30°C followed by RNA extraction using DirectZolTM (Zymo Research®) kits as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants for each sample were carefully transferred to RNase-free tubes for purification by the Direct-Zol RNA Miniprep Plus RNA purification kit (Zymo Research), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... but modified by inclusion of an additional RNase A treatment and a purification step using Zymo-Spin III columns (Zymo-Research). Purity of gDNA was assessed using either a Denovix DS-11 spectrophotometer or the Qubit 3 fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA from whole embryos or isolated tailbuds prepared as described above was treated with RNase-free DNase I (Zymo Research) to remove genomic DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... in 1× RNase H buffer overnight at 37 °C before immunoprecipitation and recovered using the Oligo Clean & Concentrator kit (Zymo Research). For m1A-DIP ...
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bioRxiv - Genomics 2024Quote: ... DNA was eluted in 105 µL RNAse free H2O and was further cleaned using OneStep PCR Inhibitor Removal Kit (Zymo Research). Concentrations for all DNA samples were measured using Qubit 3.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... digested with RNase and Proteinase K and the DNA cleaned and concentrated using ChIP DNA cleanup and concentrator kit (Zymo research Catalog# D5201) according to manufacturer instructions ...
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bioRxiv - Genomics 2021Quote: RNA from human RPTECs was extracted using the Direct-zol MicroPrep Plus Kit (Zymo) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Cultured or purified human cells were lysed directly in RNA lysis buffer (Zymo Research) and homogenized by pipetting ...
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bioRxiv - Genomics 2021Quote: ... The resulting pellet was resuspended in 100 uL RNAse free water and cleaned using the Zymo Research Quick-RNA Mini-Prep Kit and DNase1 treatment (Cat # R1054, Zymo Research Corporation, Irvine, CA) per manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: Total nucleic acids were extracted from 300 μL of nasopharyngeal samples and eluted with 60 μL sterile RNase-free water using a commercial kit (Zymo total nucleic acid, USA). Total NA concentration was assayed by Qubit RNA HS Assay Kit (Thermo Fisher Scientific ...