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Citations for Zymo Research :
1 - 50 of 410 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... GATA-3 and RORC were generated by PCR with ZymoTaq PCR Master Mix (Zymo Research) using the primers listed in Table 1 ...
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bioRxiv - Microbiology 2024Quote: ... the PCR master mix has been treated with 0.02% (v/v) of DNAse I 1 U/mL (Zymo) at room temperature for 15 minutes ...
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bioRxiv - Genomics 2020Quote: ... After PCR amplification with a Phusion Hot-start master mix and purification with a DNA Clean & Concentrator-5 kit (Zymo Research, D4013), probes were T7 amplified with a HiScribe T7 Quick High Yield RNA Synthesis Kit (New England BioLabs ...
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bioRxiv - Genomics 2023Quote: ... 10 μL of 2X ZymoTaq PCR Mix (Zymo Research), PCR primers ...
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bioRxiv - Biochemistry 2021Quote: 96-well PCR plates with 50 µL of DH10B ‘Mix & Go Competent Cells’ (prepared using Zymo T3001) in each well were prepared beforehand so that high-throughput transfers could be done using multichannel pipettes or the Tecan Fluent ...
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bioRxiv - Immunology 2021Quote: ... Amplicons were purified directly from PCR reaction mix by using DNA Clean & Concentrator Kits (Zymo Research, #D4013) or recovered from agarose gel by using Zymoclean Gel DNA Recovery Kit (Zymo Research ...
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bioRxiv - Molecular Biology 2019Quote: ... Mix&Go transformation reagents and Mix&Go JM108 chemically competent cells were obtained from Zymo Research (Irvine ...
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bioRxiv - Bioengineering 2022Quote: ... Mix and Go competent cells (Zymo Research), QIAprep Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2022Quote: ... The ligation mix was purified (Zymo Research) and 2 μL of the purified reaction mix was used to transform into 50 μL of chemically competent E ...
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bioRxiv - Plant Biology 2021Quote: ... coli (Zymo Research Mix & Go! Competent Cells), PCR-positive colonies were used for plasmid purification before Sanger sequencing (Eurofins ...
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bioRxiv - Systems Biology 2023Quote: ... 3 μl of reaction mix were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Molecular Biology 2024Quote: ... or Mix & Go Stbl3 competent cells (Zymo Research). Following standard plasmid DNA preparation ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR product was purified by PCR purification kit (Zymo Research, D4013) and sent to Azenta Life Sciences for Sanger sequencing ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were purified using Zymo PCR purification kit (Zymo Research) and directly transformed into Stellar™ chemical competent cells (Takara Bio ...
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bioRxiv - Microbiology 2021Quote: ... coli competent cells Mix & Go Zymo 5α (Zymo research) according to product manuals ...
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bioRxiv - Microbiology 2022Quote: ... Ligations were transformed into competent Mix&Go! (Zymo Research) Top10 E ...
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bioRxiv - Molecular Biology 2023Quote: ... 5hmdCTP/dGTP/dATP/dTTP mix was purchased from Zymo Research ...
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bioRxiv - Synthetic Biology 2021Quote: ... Transformation was performed using the Mix & Go kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli Mix & Go! Competent Cells-Zymo 10B (Zymo Research, T3020). For assemblies using destination vectors possessing the R6Kγ ORI ...
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bioRxiv - Bioengineering 2024Quote: ... and bacterial transformations (Mix & Go! Competent Cells - DH5 Alpha, Zymo). DNA constructs were sequence confirmed by Sanger Sequencing (Elim Bio ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were PCR purified using the DNA Clean and Concentrator kit (Zymo Research), then transformed into One Shot TOP10 competent cells (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2020Quote: ... PCR products were pooled and cleaned up with the Zymo PCR Cleanup Kit (Zymo) before performing 2% agarose gel separation and extraction with the Zymo Gel Extraction Kit following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR amplicons were purified using the Zymo PCR purification kit® (Zymo Research©). Plasmid constructions were performed using In-fusion® HD-cloning kit (Takara© ...
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bioRxiv - Microbiology 2024Quote: ... Correct amplicons were isolated by Zymo PCR purification kit (Zymo Research). The PCR products were cloned into a PCR linearized pEcgRNA-guide plasmid to make the pEcgRNA-guide-RT plasmid using In-fusion® HD-cloning kit (Takara Bio ...
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bioRxiv - Microbiology 2022Quote: ... The second endpoint PCR amplification was subjected to a PCR DNA purification kit (Zymo Research) and the DNA was submitted for sequencing as described by Christensen and colleagues ...
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bioRxiv - Genomics 2021Quote: ... gDNA was purified prior to PCR using the Zymo OneStep PCR Inhibitor Removal Kit (Zymo), per the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: ... we used DH5alpha Mix & Go Competent Cells (Zymo Research, cat. T3007) in all the above clonings except for the his-72::GFP repair template which required ccdB resistant bacteria for which we used One Shot ccdB Survival (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... coli generated from the Mix and Go Transformation Buffer kit (ZYMO). Plasmids were sequenced for validation.
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bioRxiv - Systems Biology 2024Quote: ... 2uL was transformed into 10beta Mix-and-Go chemicompetent cells (Zymo), and cells were plated to select on LB+Carb plates ...
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bioRxiv - Genetics 2023Quote: For transformation into Zymo Mix & Go DH5α competent cells (Zymo T3007), 100 µL of cells were thawed on ice per reaction ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and potential PCR inhibitors were removed with the OneStep-96TM PCR Inhibitor Removal Kit (Zymo Research). RNA concentrations were quantified with the Qubit RNA Assay kit (Life Technologies ...
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bioRxiv - Genetics 2020Quote: ... gDNA was purified prior to PCR using the Zymo OneStep PCR Inhibitor Removal Kit (Zymo, D6030), per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... After PCR product purification (Zymo Research), the fragments of interest were inserted into linearized ZP446 using Gibson Assembly ...
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bioRxiv - Microbiology 2021Quote: ... PCR inhibitors were then removed from the extracted DNA using the OneStep PCR inhibitor removal kit (Zymo) following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2022Quote: ... 15 μl of PCR products were pooled and purified using PCR Purification Kit (D4013, Zymo Research Co.). Depending on the amplicon ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using the Zymogen Clean and Concentrator PCR-cleanup kit (Zymo Research, Irvine, CA), and the recovered DNA fragments were ligated together to form closed circular DNA using T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Genomics 2020Quote: ... 2.5 μl of 10 mM 5-Methylcytosine dNTP mix (Zymo Research, D1030), and incubated at 15 °C for one hour ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Products were transformed into Mix and Go! DH5 alpha competent cells (Zymo). Clones were selected by ampicillin resistance on Amp-LB plates (60mg/mL) ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli DH5α (Zymo Mix & Go E. coli Transformation Kit, Zymo Research #T3001). Each construct was verified by Sanger sequencing (Australian Genome Research Facility ...
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bioRxiv - Microbiology 2022Quote: ... coli XL1-Blue Mix & Go competent cells (Zymo Research, cat. no. T3002) or E ...
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bioRxiv - Neuroscience 2023Quote: ... We transformed infusion reactions into Mix N’ Go (Zymo Research catalog # T3001) chemically competent Stbl3 E ...
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bioRxiv - Biophysics 2023Quote: ... coli Mix&Go Transformation kit with Zymobroth following manufacturer’s instructions (Zymo, T3001), hereafter referred to as Home-Made Competent Cells (HMCC) ...
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bioRxiv - Molecular Biology 2023Quote: ... of 0.4–0.6 and made competent using a Mix & Go kit (Zymo). Immediately after ...
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bioRxiv - Microbiology 2024Quote: ... The three PCR fragments were purified (Zymo DNA Clean and Concentrator kit ...
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bioRxiv - Developmental Biology 2023Quote: ... Obtained sgRNA mix was purified using RNA Clean and Concentrator-5 (Zymo Research). Injections were performed as before with the exception that 60 μM sgRNA mix was combined with diluted 10 μM Cas9 protein to reach final ∼5 μM RNP complex concentration (molar ratio 6:1 ...
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bioRxiv - Microbiology 2024Quote: ... and transformed into BW25113 using a Mix and Go Transformation kit (Zymo Research) as per the manufacturer’s recommended protocol ...
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bioRxiv - Genetics 2023Quote: ... Plasmids were then transformed into Zymo Mix & Go DH5α Competent Cells (Zymo T3007) for verification and amplification before re-purification and transformation into MG1655 E ...
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bioRxiv - Bioengineering 2021Quote: Colony PCR was performed on bacterial cultures and the PCR product purified using the DNA Clean and Concentrator kit (Zymo). Purified PCR products were sequenced at Genewiz (USA).
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bioRxiv - Molecular Biology 2023Quote: ... PCR product sizes were verified on a 1% agarose gel prior to PCR clean up (DNA Clean & Concentrator-5, Zymo). A DNA fragment for transcription of lpp (lpp sequence preceded by aforementioned T7 transcription-enabling sequence ...
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bioRxiv - Molecular Biology 2023Quote: The PCR products containing the aforementioned various RNA constructs were cleaned using a PCR DNA clean up kit (Zymo Research), and used in Gibson assembly reactions (5x Gibson buffer ...