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Citations for Zymo Research :
1 - 50 of 840 citations for Neuronal acetylcholine receptor subunit alpha 5 NACHRA5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... coli DH5 Alpha Competent Cells (Zymo Research) per the manufacturer’s instructions and isolated using a QIAprep Spin Miniprep Kit (QIAGEN) ...
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bioRxiv - Bioengineering 2024Quote: ... and bacterial transformations (Mix & Go! Competent Cells - DH5 Alpha, Zymo). DNA constructs were sequence confirmed by Sanger Sequencing (Elim Bio ...
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bioRxiv - Systems Biology 2019Quote: ... SD -His with uracil and 5FOA (Zymo Research) was used to analyze the phase separation effects of a toxic Ura3p activity.
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Products were transformed into Mix and Go! DH5 alpha competent cells (Zymo). Clones were selected by ampicillin resistance on Amp-LB plates (60mg/mL) ...
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bioRxiv - Genomics 2022Quote: ... were synchronized in either G1 with alpha-factor (Zymo Research, CAT. Y1001), or in late anaphase ...
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bioRxiv - Pathology 2023Quote: ... Calculations of alpha and beta diversity were done by standard methodology by Zymo Research.
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bioRxiv - Genetics 2023Quote: Neuronal cell pellets were used to isolate the total RNA using Quick-RNA MiniPrep Kit (Zymo Research #R1054) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from HEK293 and CDK9as cells using a Quick-RNA Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were propagated by transforming Escherichia Coli (Mix&Go! Competent Cells - DH5 Alpha, Zymo Research) using standard procedures ...
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bioRxiv - Bioengineering 2020Quote: ... Total RNA was extracted from mouse tissues and HEK293 cells using Quick-RNA MiniPrep Kit (ZYMO Research, Irvine, CA). Five μg of treated RNA was used as template for first-strand cDNA synthesis by using RevertAid RT Reverse Transcription Kit (Life Technologies ...
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bioRxiv - Systems Biology 2023Quote: ... 3 μl of reaction mix were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Biochemistry 2020Quote: ... then loaded directly onto a Ni-NTA protein miniprep column (His Spin Protein Miniprep, Zymo Research). Protein was washed following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 5-Fluoroorotic acid (5-FOA; ZYMO RESEARCH F9003), or doxycycline (Sigma D9891) ...
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bioRxiv - Microbiology 2019Quote: ... The cells were harvested and the protein was purified using His-Spin Protein Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... the DNMT3A KO and WT HEK293 cells were cultured and RNA samples were extracted using Direct-zol RNA kit (Zymo Research; Irvine, CA, USA). Then cDNA was synthesized according to the protocol of the RT-PCR kit (Takara ...
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bioRxiv - Bioengineering 2021Quote: ... Selection for pyrF mutations was performed with 5-fluoroorotic acid (5-FOA, Zymo Research, part number F9001-5) at a final concentration of 0.5 mg/ml ...
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bioRxiv - Zoology 2024Quote: ... These amplified products were then purified as above and restriction enzyme digested CCHa1R and CCHa2R sequences were ligated into pcDNA3.1+ and pBudCE4.1 mammalian expression vectors followed by bacterial transformation and plasmid DNA purification to obtain a large quantity of receptor constructs using ZymoPURE II Plasmid Midiprep Kit (Zymo Research, Tustin, CA, USA) following the manufacturer recommendation ...
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bioRxiv - Systems Biology 2023Quote: ... The reactions were incubated for 15 min at 50 °C and 3 μl were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Microbiology 2022Quote: The ZymoBIOMICS human fecal reference sample (Zymo D6323) was thawed at room temperature and 100 μL was taken and centrifuged at 35g for 20 minutes to separate cells from large fecal particles ...
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bioRxiv - Molecular Biology 2020Quote: ... the shuffle strain transformed with plasmid expressing mutant ribosomal protein products was grown on SDC plates containing 0.5 mg/mL 5-fluoroorotic acid (5-FOA, #F9001-5, Zymo Research) and a strain lacking URA3 was isolated.
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bioRxiv - Plant Biology 2023Quote: ... Relative quantification of 5-methylcytosine (5-mC) over time was determined using the 5-mC DNA ELISA Kit (Zymo Research), using 100 ng of genomic DNA per sample as input ...
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bioRxiv - Microbiology 2022Quote: ... After collection of TI and TS fractions 100 µl of His-affinity gel (i.e., Ni-NTA Magnetic Agarose Beads) (ZYMO Research) and 10 µl of protease inhibitor cocktail (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... Methylated and unmethylated human gDNAs (Zymo Research, Irvine, CA) were bisulfite-converted using the EZ DNA Methylation-Gold kit (Zymo Research ...
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bioRxiv - Genomics 2021Quote: ... The Human Methylated & Non-methylated DNA Set (ZYMO Research) was used to generate DNA methylation standards ...
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bioRxiv - Cancer Biology 2022Quote: ... was used for the estimation of global 5-hmC and 5-mC levels by ELISA using the Quest 5-hmC ELISA kit and the 5-mC DNA ELISA kit (Zymo research Inc, USA) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... or a combination of TRIzol and the RNA Clean & Concentrator-5 (RCC-5) kit (Zymo Research), according to the Zymo instructions for combination Trizol/Zymo-Spin-Column RNA isolation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.075% 5-fluorooritic acid (Zymo Research)) ...
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bioRxiv - Genomics 2020Quote: ... We verified the presence of high molecular weight DNA products in the samples before purifying nucleic acids by Zymo DNA Clean & Concentrator-5 (ZYMO Research).
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bioRxiv - Synthetic Biology 2020Quote: ... and DCC-5 columns (Zymo Research). The sublibraries were pooled and sequenced on a single lane of a HiSeq4000 (Illumina) ...
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bioRxiv - Immunology 2020Quote: ... whole zebrafish larvae or human biopsies using TRI Reagent (Zymo Research) or TRIzol (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... and then the fragmented DNAs were purified with DNA Clean & Concentrator-5 Kit (Zymo Research, DCC-5) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... the cell suspension was mixed with 5 µl of 5 U/μl Zymolyase (Zymo Research, catalog no. E1005) and incubated for 1 h at 37°C with agitation ...
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bioRxiv - Neuroscience 2019Quote: ... RNA Clean & Concentrator-5 (Zymo Research # R1013) was performed according to manufacturer’s protocol and RNA was eluted into 12 μL of RNase-free water ...
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bioRxiv - Genomics 2021Quote: ... RNA Clean & Concentrator-5 kit from Zymo Research (R1015 ...
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bioRxiv - Bioengineering 2021Quote: ... 5 μL of Zymolyase (Zymo Research, E1005) was added to digest yeast cell walls and incubated at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... A DCC-5 kit (Zymo Research, D4014) was used to purify DNA (20-μl elution) ...
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bioRxiv - Bioengineering 2021Quote: ... 10 mL 5-Fluoroorotic Acid (Zymo Research), 50 mg uracil (MP Biomedicals 103204) ...
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bioRxiv - Microbiology 2023Quote: ... and RNA Clean & Concentrator-5 kit (Zymo). rRNA removal was performed using the QIAseq FastSelect- 5S/16S/23S kit (Qiagen) ...
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bioRxiv - Genomics 2023Quote: ... 0.08 μL 40mM 5-hydroxymethyl-dCTP (ZYMO), 0.16 μL Q5 (NEB M0491S) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl RNA from total or immunopurified ribosomes were 3’ dephosphorylated and 5’ phosphorylated as described above 30 and was selectively recovered with RNA Clean and Concentrator-5 (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The DNA digestion efficiency was monitored by using 100 ng 5-methylcytosine and 5-hydroxymethylcytosine DNA standard sets (Zymo Research) in parallel under identical conditions ...
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bioRxiv - Synthetic Biology 2022Quote: ... and fragment mixes for homology dependent assembly (Figure 5) were cleaned and concentrated with DNA Clean & Concentrator-5 columns (Zymo), and DNA was eluted with 10 μL molecular grade water ...
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bioRxiv - Genomics 2020Quote: ... and RNA Clean and Concentrator-5 (Zymo Research). The quality of extracted RNA was checked by Agilent Bioanalyzer on Agilent RNA 6000 Pico Chips (Agilent technologies ...
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bioRxiv - Genetics 2019Quote: ... RNA Clean and ConcentratorTM −5 (R1016, Zymo Research) was applied after each step in the RNA preparation procedure to clean up and concentrate the RNA ...
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bioRxiv - Synthetic Biology 2021Quote: ... The crude DNA extracts were purified by Zymo DNA Clean & Concentrator™-5 kit (Zymo Research) for NGS sequencing.
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bioRxiv - Evolutionary Biology 2020Quote: ... purified with DNA Clean & Concentrator 5 (Zymo Research), and sequenced by Sanger technology in both directions at Macrogen Europe ...
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bioRxiv - Neuroscience 2022Quote: ... and RNA Clean & ConcentratorTM-5 (Zymo Research, #R1014). Jun expression was analyzed by qRT-PCR with the primers ...
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bioRxiv - Genomics 2021Quote: DNA was recovered with Concentrator 5 columns (Zymo) and prepared for sequencing using MicroPlex Library Preparation Kit v2 (C05010012 ...
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bioRxiv - Microbiology 2022Quote: ... using RNA Clean & Concentrator-5 (Zymo Research Inc.) for the first cleanup after the RiboFree® depletion step (according to Appendix E) ...
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bioRxiv - Microbiology 2022Quote: ... cleaned up (RNA Clean & Concentrator-5, Zymo Research), quantified (Qubit RNA BR Assay Kit ...