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Citations for Zymo Research :
1 - 47 of 47 citations for NT proBNP Protein Recombinant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... RNA clean-up and separation into small (17-200 nt) and large (>200 nt) RNA fractions was performed using RNA Clean & Concentrator columns (Zymo Research). The large RNA fraction was fragmented according to the protocol by Pfeifer-Sancar et al ...
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bioRxiv - Plant Biology 2019Quote: ... RNA greater than 17 nt was purified first (Zymo Research R1017), and then RNA smaller than 200 nt was enriched (Zymo Research R1015) ...
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bioRxiv - Microbiology 2022Quote: ... The size of 150-400 nt was cut and recovered by Zymo DNA agarose gel extraction kit (Zymo Research) ...
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bioRxiv - Plant Biology 2019Quote: ... and then RNA smaller than 200 nt was enriched (Zymo Research R1015). Next ...
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bioRxiv - Genomics 2022Quote: ... The ligated products (≥200 nt) were then purified by RNA Clean & Concentrator (Zymo Research, R1016). Subsequent steps including dU adapter cleavage with CutSmart® buffer and column purification were stated in detail above ...
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bioRxiv - Genomics 2023Quote: ... RNA > 17 nt was isolated with the RNA Clean & Concentrator-25 kit (Zymo Research, R1017) and separated on 15% urea-TBE gels (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... RNA > 17 nt was isolated with the RNA Clean & Concentrator-25 kit (Zymo Research, R1017) and separated on 15% urea-TBE gels (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... and were then size-selected to isolate RNA ≥ 200 nt (DNA Clean & Concentrator™-5, Zymo Research) before reverse transcription using All-in-One cDNA Synthesis SuperMix (Bimake ...
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bioRxiv - Plant Biology 2019Quote: ... RNA greater than 200 nt was extracted using a Zymo RNA Clean & Concentrator kit (Zymo Research R1017). The obtained RNA was checked with a Bioanalyzer (Agilent ...
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bioRxiv - Genetics 2019Quote: ... RNAs longer than 200 nt were selectively recovered using the RNA Clean & Concentrator-5 kit (Zymo Research). RNA samples were fragmented and reverse transcribed ...
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bioRxiv - Synthetic Biology 2023Quote: ... 357-nt mini-PTC RNA constructs were purified with RNA Clean and Concentrator-25 columns (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... the reaction was purified and size selected to > 200 nts using Zymo Clean and Concentrator-5 (Zymo #D4013) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... followed by clean-up and enrichment of RNA > ∼150 nt using RNA clean and concentrator (Zymo Research #R1013). DNase activity was tested beforehand to ensure DNA digestion ...
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bioRxiv - Genomics 2023Quote: ... followed by gel size selection to isolate ribosome protected fragments (26-31 nt) (Zymo Research, cat. no. R1070). Following end-repair phosphorylation of RNA molecules ...
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bioRxiv - Microbiology 2021Quote: ... and RNA >200 nt was purified using an RNA Clean & Concentrator-5 Kit per the manufacturer’s instructions (Zymo Research). Ribosomal RNA was depleted using a MICROBExpress Bacterial mRNA Enrichment Kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... The reaction was purified and size selected to >200 nts using Zymo RNA Clean and Concentrator-5 (Zymo #R1013) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... The RNA was size-selected to ≥200 nts using the Zymo RNA Clean and Concentrator-5 kit (Zymo #R1013) according to manufacturer’s protocol and eluted in 6 µl of nuclease-free water.
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bioRxiv - Plant Biology 2020Quote: ... and RNA greater than 17 nt was isolated following manufacturer’s instructions with the Zymo RNA clean and concentrator kit (Zymo Research; R1017). After checking digestion quality ...
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bioRxiv - Plant Biology 2020Quote: ... RNA less than 200 nt was isolated following manufacturer’s instructions with the Zymo RNA clean and concentrator kit (Zymo Research; R1015). Next ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were run on an UltraPure Agarose (Thermo Fischer Scientific) gel and cDNA fragments larger than 500 nt were purified using the Zymoclean Large Fragment DNA Recovery Kit (Zymo Research). The library was prepared using the ONT 1D kit (SQK-LSK108 ...
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bioRxiv - Genetics 2022Quote: ... Resulting cDNA was separated on a 4% agarose gel and the 160–190 nt long fragments were extracted and gel purified using the Zymoclean Gel DNA Recovery Kit (D4008, ZYMO RESEARCH). Small RNA Libraries were sequenced in triplicates on a NovaSeq S1 SR100 mode (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... gel fragments corresponding to 190 – 350 nts in DNA size were excised and libraries extracted using the Zymoclean Gel DNA Recovery Kit (D4007, Zymo Research). DNA concentrations were measured using the Qubit dsDNA HS Assay Kit (Q32854 ...
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bioRxiv - Genomics 2022Quote: ... The corresponding amount of polyA RNA were incubated with 1X fragmentation buffer at 95 °C for 1 minute to generate ~300 nt of RNA fragment followed by column purification (Zymo Research, R1016) as described before [23] ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting fraction was loaded on a 20% denaturing PAGE gel after which a band of approximately 55-60 nt (Supplementary figure 2A) was excised and purified from gel using the ZR small-RNA PAGE Recovery Kit (Zymo Research, USA). Small RNAs were prepared by GenXPro (GenXPro GmbH ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting fraction was loaded on a 20% denaturing PAGE gel after which a band of approximately 55-60 nt (Supplementary figure 2A) was excised and purified from gel using the ZR small-RNA PAGE Recovery Kit (Zymo Research, USA).
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bioRxiv - Molecular Biology 2021Quote: ... to block the 3’-ends from ligation and were then size-selected to 200 ≥ nt (DNA Clean & Concentrator™-5, Zymo Research). 5’ adaptors were attached using T4 RNA ligase (Ambion ...
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bioRxiv - Genomics 2023Quote: Total RNA greater than 200 nt was purified from 100 µL of the lysates described above using the RNA Clean & Concentrator-25 kit (Zymo Research, R1017) as previously described (Hsu et al. ...
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bioRxiv - Genomics 2023Quote: Total RNA greater than 200 nt was purified from 100 µL of the lysates described above using the RNA Clean & Concentrator-25 kit (Zymo Research, R1017) as previously described (Hsu et al. ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... small RNAs ranging from 17-to-25 nt were excised and recovered using the ZR small-RNA PAGE Recovery Kit (Zymo Research, #R1070). TraPR ...
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bioRxiv - Biophysics 2023Quote: ... Recombinant bacmid DNA was purified using the ZR Bac DNA Miniprep Kit (Zymo Research) and correctness of the transposed sequence was analyzed by PCR using pUC/M13 primers ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Recombinant reporter constructs were isolated using the ZymoPURE™ Plasmid Miniprep Kit (Zymo Research, #D4210), following the manufacturer’s protocol with the following minor modifications ...
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bioRxiv - Biochemistry 2020Quote: ... then loaded directly onto a Ni-NTA protein miniprep column (His Spin Protein Miniprep, Zymo Research). Protein was washed following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... coli TOP10 cells were propagated in LB medium (ampicillin, 100 µg/ml) and used for recombinant plasmid DNA isolation (Zypy Plasmid Miniprep, Zymo Research). Prior to plasmid transformation to expression host E ...
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bioRxiv - Synthetic Biology 2024Quote: ... the constructed donor DNA fragment and recombinant plasmid pYlCas9 were simultaneously transformed into the targeted strain using the Frozen-EZ yeast transformation II Kit (Zymo Research). The amounts of donor DNA and plasmid pYlCas9 used were 1 μg and 500 ng ...
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bioRxiv - Microbiology 2019Quote: ... The cells were harvested and the protein was purified using His-Spin Protein Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Protein degradation was performed by adding Proteinase K (Zymo) and Qiagen Protease (Qiagen) ...
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bioRxiv - Cell Biology 2023Quote: ... ChIP-DNA was prepared using ZymoMag Protein A beads (Zymo Research). Input samples were also prepared using 10% of chromatin samples ...
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bioRxiv - Immunology 2022Quote: pAd/S1RS09cg proteins were amplified and purified using ZymoPURE II plasmid maxiprep kit (Zymo Research). For Expi293 cell transfection ...
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bioRxiv - Biochemistry 2023Quote: ... and the DNAs were purified away from protein using a DNA Clean & Concentrator kit (Zymo Research). Both 5’ ends of 1 pmole of the CRISPR leader and array fragments were then labelled with 32P ...
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bioRxiv - Neuroscience 2022Quote: ... Then the nucleic-acid bound protein isolation was performed using buffers from the Quick-RNA Miniprep Kit (Zymo Research) and the Zymo-Spin IIICG Column (Zymo Research) ...
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bioRxiv - Cell Biology 2023Quote: ... Endogenous RNA cross-linked to bait protein was recovered from the beads using the QuickRNA Microprep Kit (Zymo Research). Complementary DNA was synthesized using the SuperScript III First-Strand Synthesis kit (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... DNA was captured on protein A/G agarose beads and purified using the ChIP DNA Clean & Concentrator kit (Zymo) and used for qPCR ...
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bioRxiv - Molecular Biology 2020Quote: ... the shuffle strain transformed with plasmid expressing mutant ribosomal protein products was grown on SDC plates containing 0.5 mg/mL 5-fluoroorotic acid (5-FOA, #F9001-5, Zymo Research) and a strain lacking URA3 was isolated.
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bioRxiv - Biochemistry 2022Quote: ... the protein solutions were applied to a spin column containing Strep-Tactin XT Superflow suspension (Zymo Research, Irvine, CA, USA). After washing the column in a buffer containing 50 mM HEPES-KOH (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... Combination of the two plasmids encoding the desired fusion proteins were transformed into Y2HGold cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Selection for growth was carried out on selective drop-out plates lacking leucine and tryptophan (SD-Leu-Trp ...
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bioRxiv - Plant Biology 2024Quote: ... was used to pull-down the protein/DNA complexes and DNA was purified using ChIP Clean-up and Concentrator kit (Zymo Research). Libraries were constructed using Ultra II DNA kit (NEB).
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bioRxiv - Bioengineering 2022Quote: Yeast competent cells were transformed with polycistronic constructs containing fluorescent proteins using the Frozen-EZ yeast transformation II kit (Zymo Research, Irvine, CA) according to the manufacturer’s protocol ...